scholarly journals Chromatographic Studies of Protein-Based Chiral Separations

Separations ◽  
2016 ◽  
Vol 3 (3) ◽  
pp. 27 ◽  
Author(s):  
Cong Bi ◽  
Xiwei Zheng ◽  
Shiden Azaria ◽  
Sandya Beeram ◽  
Zhao Li ◽  
...  
Keyword(s):  
Chiral Separations

Recent Trends in Chiral Separations by 2D-HPLC

2021 ◽  
Author(s):  
Imran Ali ◽  
Mohd. Suhail ◽  
Hassan Y. Aboul-Enein ◽  
Tatiana Kon’kova
Keyword(s):  
Chiral Separations ◽  
Recent Trends

scholarly journals Recent Advances in Chiral Analysis of Proteins and Peptides

Separations ◽  
2021 ◽  
Vol 8 (8) ◽  
pp. 112
Author(s):  
Marine Morvan ◽  
Ivan Mikšík
Keyword(s):  
Amino Acids ◽  
Stationary Phases ◽  
Enzymatic Digestion ◽  
Chiral Separations ◽  
Biological Compounds ◽  
Health And Disease ◽  
Electrophoretic Techniques ◽  
A Site ◽  
Future Work ◽  
Proteins And Peptides

Like many biological compounds, proteins are found primarily in their homochiral form. However, homochirality is not guaranteed throughout life. Determining their chiral proteinogenic sequence is a complex analytical challenge. This is because certain D-amino acids contained in proteins play a role in human health and disease. This is the case, for example, with D-Asp in elastin, β-amyloid and α-crystallin which, respectively, have an action on arteriosclerosis, Alzheimer's disease and cataracts. Sequence-dependent and sequence-independent are the two strategies for detecting the presence and position of D-amino acids in proteins. These methods rely on enzymatic digestion by a site-specific enzyme and acid hydrolysis in a deuterium or tritium environment to limit the natural racemization of amino acids. In this review, chromatographic and electrophoretic techniques, such as LC, SFC, GC and CE, will be recently developed (2018–2020) for the enantioseparation of amino acids and peptides. For future work, the discovery and development of new chiral stationary phases and derivatization reagents could increase the resolution of chiral separations.

scholarly journals Chiral Selectors in Capillary Electrophoresis: Trends During 2017–2018

Molecules ◽  
2019 ◽  
Vol 24 (6) ◽  
pp. 1135 ◽  
Author(s):  
Raymond B. Yu ◽  
Joselito P. Quirino
Keyword(s):  
Capillary Electrophoresis ◽  
Molecular Mass ◽  
Chiral Separation ◽  
Chiral Separations ◽  
Water Soluble ◽  
Resonance Spectroscopy ◽  
Electrokinetic Chromatography ◽  
Chiral Selectors ◽  
Analytical Technique ◽  
Pharmaceutical Industries

Chiral separation is an important process in the chemical and pharmaceutical industries. From the analytical chemistry perspective, chiral separation is required for assessing the fit-for-purpose and the safety of chemical products. Capillary electrophoresis, in the electrokinetic chromatography mode is an established analytical technique for chiral separations. A water-soluble chiral selector is typically used. This review therefore examines the use of various chiral selectors in electrokinetic chromatography during 2017–2018. The chiral selectors were both low and high (macromolecules) molecular mass molecules as well as molecular aggregates (supramolecules). There were 58 papers found by search in Scopus, indicating continuous and active activity in this research area. The macromolecules were sugar-, amino acid-, and nucleic acid-based polymers. The supramolecules were bile salt micelles. The low molecular mass selectors were mainly ionic liquids and complexes with a central ion. A majority of the papers were on the use or preparation of sugar-based macromolecules, e.g., native or derivatised cyclodextrins. Studies to explain chiral recognition of macromolecular and supramolecular chiral selectors were mainly done by molecular modelling and nuclear magnetic resonance spectroscopy. Demonstrations were predominantly on drug analysis for the separation of racemates.

Optimum operating conditions for chiral separations in liquid chromatography

The Analyst ◽  
1999 ◽  
Vol 124 (5) ◽  
pp. 713-719 ◽  
Author(s):  
R. P. W. Scott ◽  
Thomas E. Beesley
Keyword(s):  
Liquid Chromatography ◽  
Chiral Separations ◽  
Operating Conditions ◽  
Optimum Operating

Chiral separations of β-blocking drug substances using the Pirkle-type α-Burke 1 chiral stationary phase

1997 ◽  
Vol 757 (1-2) ◽  
pp. 65-71 ◽  
Author(s):  
Poul Vibholm Petersen ◽  
Jens Ekelund ◽  
Lars Olsen ◽  
Sanna Vinding Ovesen
Keyword(s):  
Stationary Phase ◽  
Chiral Stationary Phase ◽  
Chiral Separations ◽  
Blocking Drug ◽  
Drug Substances
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