scholarly journals Association between Transcription Factor 7-like-2 Polymorphisms and Type 2 Diabetes Mellitus in a Ghanaian Population

Sci ◽  
2021 ◽  
Vol 3 (4) ◽  
pp. 40
Author(s):  
Christian Obirikorang ◽  
Evans Asamoah Adu ◽  
Enoch Odame Anto ◽  
Emmanuel Acheampong ◽  
Lawrence Quaye ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Lipid Analysis ◽  
Additive Model ◽  
Nucleotide Polymorphisms ◽  
Tcf7l2 Gene ◽  
Salting Out ◽  
Blood Samples

Type 2 diabetes mellitus (T2DM) has been strongly associated with single nucleotide polymorphisms (SNPs) in the TCF7L2 gene. This study investigated the association between rs12255372, rs7903146 in the TCF7L2 gene and T2DM in a Ghanaian population. A case-control study design was used for this study. A total of 106 T2DM patients and 110 control participants were selected. Basic data collected included body mass index, blood pressure and socio-demographics. Fasting blood samples were collected and processed for: serum lipid analysis, plasma glucose estimation and plasma HbA1c estimation. Parts of the whole blood samples were used for DNA extraction using a modified salting-out method. Common and allele-specific primers were designed for genotyping using the Modified Tetra-Primer Amplification assay. Associations were evaluated using logistic regression models. The rs7903146 risk variant was significantly associated with 2.16 vs. 4.06 increased odds for T2DM in patients <60 years vs. ≥60 years. Both rs7903146 and rs12255372 were significantly associated with increased odds of T2DM in women, overweight/obese, T2DM negative family history (T2DM-NFH) and low-HDL-C. In a multivariate model, rs7903146 but not rs12255372 was significantly associated with 2.18, 5.01 and 2.25 increased odds of T2DM, under the codominant, recessive and additive model, respectively (p < 0.05). The association between rs7903146 and rs12255372 with T2DM is more highly associated in a subgroup—women and those with T2DM-NFH, yet who have cardiometabolic risk.

scholarly journals Interplay of Transcription Factor 7-Like-2 Polymorphisms with Type 2 Diabetes Mellitus in a Ghanaian Population

2021 ◽  
Author(s):  
Christian Obirikorang ◽  
Evans Asamoah Adu ◽  
Emmanuel Acheampong ◽  
Enoch Odame Anto ◽  
Lawrence Quaye ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Family History ◽  
Lipid Analysis ◽  
Additive Model ◽  
Nucleotide Polymorphisms ◽  
Logistic Regression Models ◽  
Glucose Estimation

Abstract Background Type-2 diabetes mellitus (T2DM) have been strongly associated with single nucleotide polymorphisms (SNPs) in the TCF7L2 gene. This study investigated the interplay between rs12255372 and rs7903146 with T2DM in a Ghanaian population. Methods A case-control design was used for this study. A total of 106 T2DM patients and 110 apparently healthy control participants were selected. Basic data collected included body mass index, blood pressure and socio-demographics. Fasting blood samples were collected and used for serum lipid analysis, HbA1c, glucose estimation and DNA extraction. Common and allele-specific primers were designed for genotyping using the Modified Tetra-Primer Amplification assay. Associations were evaluated using logistic regression models. Results The rs7903146 risk variant was significantly associated with 2.16 vs 4.06 increased odds for T2DM in patients < 60 years vs ≥ 60 years. Both rs7903146 and rs12255372 were significantly associated with increased odds of T2DM in women, overweight/obese; negative T2DM family history and low-HDL-C. In a multivariate model, rs7903146 but not rs12255372 was significantly associated with 2.18, 5.01 and 2.25 increased odds of T2DM, under the codominant, recessive and additive model, respectively (p < 0.05). Haplotype analysis showed that rs7903146 and rs12255372 SNPs are weakly associated (D’=0.021; r2 = 0.0001). Conclusion The association between rs7903146 and rs12255372 with T2DM looks to be more highly associated in a subgroup- women and those without T2DM family history, yet have cardiometabolic risk.

scholarly journals Association Between Transcription Factor 7-Like-2 Polymorphisms and Type 2 Diabetes Mellitus in a Ghanaian Population

2021 ◽  
Author(s):  
Christian Obirikorang ◽  
Evans Asamoah Adu ◽  
Enoch Odame Odame ◽  
Emmanuel Acheampong ◽  
Lawrence Quaye ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Lipid Analysis ◽  
Nucleotide Polymorphisms ◽  
Logistic Regression Models ◽  
Allele Specific ◽  
Amplification Assay ◽  
Glucose Estimation

Type-2 diabetes mellitus (T2DM) have been strongly associated with single nucleotide polymorphisms (SNPs) in the TCF7L2 gene. This study investigated the association between rs12255372, rs7903146 and T2DM in a Ghanaian population. A case-control study design was used for this study. A total of 106 T2DM patients and 110 control participants were selected. Basic data collected included body mass index, blood pressure and socio-demographics. Fasting blood samples were collected and used for serum lipid analysis, HbA1c, plasma glucose estimation and DNA extraction. Common and allele-specific primers were designed for genotyping using the Modified Tetra-Primer Amplification assay. Associations were evaluated using logistic regression models. The rs7903146 risk variant was significantly associated with 2.16 vs 4.06 increased odds for T2DM in patients

scholarly journals Association between RBMS1 gene rs7593730 and BCAR1 gene rs7202877 and Type 2 Diabetes Mellitus in a Chinese Han Population

2018 ◽  
Vol 65 (3) ◽  
Author(s):  
Elena Kazakova ◽  
Meijun Chen ◽  
Esma Jamaspishvili ◽  
Zhang Lin ◽  
Jingling Yu ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Chinese Population ◽  
Additive Model ◽  
Recessive Model ◽  
Nucleotide Polymorphisms ◽  
Chinese Han ◽  
Single Nucleotide

Two recent studies found that RBMS1 gene rs7593730 and BCAR1 gene rs7202877 had relationships with type 2 diabetes. However, the association of these loci with type 2 diabetes mellitus (T2DM) has not been examined in Chinese. We performed a replication study to investigate the association of 2 susceptibility loci with T2DM in the Chinese population. We genotyped 1961 Chinese participants (991 with T2DM and 970controls) for each of the 2 single nucleotide polymorphisms (SNPs) rs7593730 in RBMS1 and rs7202877 near BCAR1 using SNPscan and examined the association of them with T2DM using logistic regression analysis. We also analyzed the correlation of the SNP allele and clinical phenotypes. In additive model, genotype association analysis of BCAR1 rs7202877 loci revealed that the homozygous of rs7202877 GG carriers had a significant decreased T2DM risk compared to homozygous carriers of TT (p= 0.038, OR 0.44, 95 % CI 0.20-0.96). In the recessive model, the genotype GG carriers had a significant decreased T2DM risk compared to GT+TT (p= 0.043, OR 0.67, 95% CI 0.46-0.99). Allele G was statistically significantly correlated with TC (mmol/L) (P=0.036) and LDL-C (mmol/L) (P=0.007). As for rs7593730, the carriers of genotype CT and TT had significantly decreased T2DM risk compared with the carriers of genotype CC (CT: CC P=0.038, OR 0.71, 95%CI 0.51-0.98; TT: CC P=0.010, OR 0.32, 95%CI 0.13-0.76). In a dominant model, TT+CT: CC (p= 0.013, OR 0.673, 95 % CI 0.49-0.92) and in a recessive model, TT: CT+CC (p= 0.019, OR 0.59, 95% CI 0.39-0.92). The T allele carriers had a significant decreased T2DM risk compared to carriers of C (p= 0.002, OR 0.65, 95 % CI 0.50-0.86). Allele T was statistically correlated with FINS (P=0.010). In conclusion, our study showed that RBMS1 gene rs7593730 and BCAR1 gene rs7202877 were significantly associated with type 2 diabetes in the Chinese population.

scholarly journals TGF-β1 Gene Polymorphism in Codon 10 +869*C/T and Codon 25 +915*G/C Positions in Iraqi Patients with Type 2 Diabetes Mellitus

2016 ◽  
Vol 10 (2) ◽  
pp. 47-51
Author(s):  
Ihsan A. Hussein
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Protective Effect ◽  
Control Sample ◽  
Amplification Refractory Mutation System ◽  
Blood Samples ◽  
Increased Risk ◽  
Tgf Β1

This study included 50 blood samples that were collected from patients with age ranged between 35-65 years. Thirty samples were collected from patients with Type 2 Diabetes Mellitus (T2DM), while 20 blood samples were collected from healthy individuals as a control sample. The polymorphism results of TGF-β1 gene in codon 10: +869*C/T position by using amplification refractory mutation system (ARMS-PCR) showed that the T allele was suggested to have a protective effect, while C allele was associated with an increased risk of T2DM. The TT and CT were suggested to have a protective effect, while CC genotype was associated with an increased risk of T2DM. The polymorphism results of TGF-β1 gene in codon 25: +915*G/C position in samples showed that the G allele was suggested to have a protective effect, while C allele was associated with an increased risk of T2DM. The GC genotype was suggested to have a protective effect, while GG and CC genotypes were associated with an increased risk of T2DM.

Single and multi-locus association study of TCF7L2 gene variants with susceptibility to type 2 diabetes mellitus in an Iranian population

Gene ◽  
2019 ◽  
Vol 696 ◽  
pp. 88-94 ◽  
Author(s):  
Saeed Kalantari ◽  
Alireza Sharafshah ◽  
Parvaneh Keshavarz ◽  
Arash Davoudi ◽  
Razie Habibipour
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Association Study ◽  
Iranian Population ◽  
Gene Variants ◽  
Tcf7l2 Gene

Association of a common variant in TCF7L2 gene with type 2 diabetes mellitus in a Persian population

2011 ◽  
Vol 12 (2) ◽  
pp. 115-119 ◽  
Author(s):  
Abbasali Palizban ◽  
Mahnaz Nikpour ◽  
Rasoul Salehi ◽  
Mohammad-Reza Maracy
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Common Variant ◽  
Tcf7l2 Gene
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