scholarly journals Combination of On-line pH and Oxygen Transfer Rate Measurement in Shake Flasks by Fiber Optical Technique and Respiration Activity MOnitoring System (RAMOS)

Sensors ◽  
2007 ◽  
Vol 7 (12) ◽  
pp. 3472-3480 ◽  
Author(s):  
Marco Scheidle ◽  
Johannes Klinger ◽  
Jochen Büchs
Keyword(s):  
Monitoring System ◽  
Oxygen Transfer ◽  
Transfer Rate ◽  
Oxygen Transfer Rate ◽  
Optical Technique ◽  
Rate Measurement ◽  
Respiration Activity ◽  
Fiber Optical ◽  
On Line ◽  
Shake Flasks

scholarly journals Time-Resolved Monitoring of the Oxygen Transfer Rate of Chinese Hamster Ovary Cells Provides Insights Into Culture Behavior in Shake Flasks

2021 ◽  
Vol 9 ◽  
Author(s):  
Nina Ihling ◽  
Lara Pauline Munkler ◽  
Christoph Berg ◽  
Britta Reichenbächer ◽  
Johannes Wirth ◽  
...  
Keyword(s):  
Oxygen Transfer ◽  
Transfer Rate ◽  
Shake Flask ◽  
Oxygen Transfer Rate ◽  
Carbon Sources ◽  
Chinese Hamster ◽  
Respiration Activity ◽  
Time Resolved ◽  
Non Invasive ◽  
Shake Flasks

Cultivations of mammalian cells are routinely conducted in shake flasks. In contrast to instrumented bioreactors, reliable options for non-invasive, time-resolved monitoring of the culture status in shake flasks are lacking. The Respiration Activity Monitoring Respiration Activity Monitoring System system was used to determine the oxygen transfer rate (OTR) in shake flasks. It was proven that the OTR could be regarded as equal to the oxygen uptake rate as the change of the dissolved oxygen concentration in the liquid phase over time was negligibly small. Thus, monitoring the oxygen transfer rate (OTR) was used to increase the information content from shake flask experiments. The OTR of a Chinese hamster ovary cell line was monitored by applying electrochemical sensors. Glass flasks stoppered with cotton plugs and polycarbonate flasks stoppered with vent-caps were compared in terms of mass transfer characteristics and culture behavior. Similar mass transfer resistances were determined for both sterile closures. The OTR was found to be well reproducible within one experiment (standard deviation <10%). It correlated with changes in cell viability and depletion of carbon sources, thus, giving more profound insights into the cultivation process. Culture behavior in glass and polycarbonate flasks was identical. Monitoring of the OTR was applied to a second culture medium. Media differed in the maximum OTR reached during cultivation and in the time when all carbon sources were depleted. By applying non-invasive, parallelized, time-resolved monitoring of the OTR, the information content and amount of data from shake flask experiments was significantly increased compared to manual sampling and offline analysis. The potential of the technology for early-stage process development was demonstrated.

scholarly journals On-Line Control of Glucose Concentration in High-Yielding Mammalian Cell Cultures Enabled Through Oxygen Transfer Rate Measurements

2018 ◽  
Vol 13 (4) ◽  
pp. 1700607 ◽  
Author(s):  
Stephen Goldrick ◽  
Kenneth Lee ◽  
Christopher Spencer ◽  
William Holmes ◽  
Marcel Kuiper ◽  
...  
Keyword(s):  
Mammalian Cell ◽  
Cell Cultures ◽  
Glucose Concentration ◽  
Oxygen Transfer ◽  
Transfer Rate ◽  
Oxygen Transfer Rate ◽  
Mammalian Cell Cultures ◽  
On Line

scholarly journals The metabolic switch can be activated in a recombinant strain of Streptomyces lividans by a low oxygen transfer rate in shake flasks

2018 ◽  
Vol 17 (1) ◽  
Author(s):  
Ramsés A. Gamboa-Suasnavart ◽  
Norma A. Valdez-Cruz ◽  
Gerardo Gaytan-Ortega ◽  
Greta I. Reynoso-Cereceda ◽  
Daniel Cabrera-Santos ◽  
...  
Keyword(s):  
Oxygen Transfer ◽  
Transfer Rate ◽  
Recombinant Strain ◽  
Oxygen Transfer Rate ◽  
Streptomyces Lividans ◽  
Low Oxygen ◽  
Metabolic Switch ◽  
Shake Flasks

Oxygen supply in disposable shake-flasks: prediction of oxygen transfer rate, oxygen saturation and maximum cell concentration during aerobic growth

2013 ◽  
Vol 35 (8) ◽  
pp. 1223-1230 ◽  
Author(s):  
Sarah Schiefelbein ◽  
Alexander Fröhlich ◽  
Gernot T. John ◽  
Falco Beutler ◽  
Christoph Wittmann ◽  
...  
Keyword(s):  
Oxygen Saturation ◽  
Oxygen Transfer ◽  
Transfer Rate ◽  
Oxygen Supply ◽  
Cell Concentration ◽  
Oxygen Transfer Rate ◽  
Aerobic Growth ◽  
Shake Flasks ◽  
Maximum Cell ◽  
Maximum Cell Concentration

scholarly journals Investigation of poly(γ-glutamic acid) production via online determination of viscosity and oxygen transfer rate in shake flasks

2017 ◽  
Vol 11 (1) ◽  
Author(s):  
Lena Regestein née Meissner ◽  
Julia Arndt ◽  
Thomas G. Palmen ◽  
Tim Jestel ◽  
Hitoshi Mitsunaga ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Oxygen Transfer ◽  
Acid Production ◽  
Transfer Rate ◽  
Oxygen Transfer Rate ◽  
Shake Flasks ◽  
Glutamic Acid Production
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