scholarly journals Error Analysis of the Combined-Scan High-Speed Atomic Force Microscopy

Sensors ◽  
2021 ◽  
Vol 21 (18) ◽  
pp. 6139
Author(s):  
Lu Liu ◽  
Ming Kong ◽  
Sen Wu ◽  
Xinke Xu ◽  
Daodang Wang

A combined tip-sample scanning architecture can improve the imaging speed of atomic force microscopy (AFM). However, the nonorthogonality between the three scanners and the nonideal response of each scanner cause measurement errors. In this article, the authors systematically analyze the influence of the installation and response errors of the combined scanning architecture. The experimental results show that when the probe in the homemade high-speed AFM moves with the Z-scanner, the spot position on the four-quadrant detector changes, thus introducing measurement error. Comparing the experimental results with the numerical and theoretical results shows that the undesired motion of the Z-scanner introduces a large error. The authors believe that this significant error occurs because the piezoelectric actuator not only stretches along the polarization direction but also swings under nonuniform multifield coupling. This article proposes a direction for further optimizing the instrument and provides design ideas for similar high-speed atomic force microscopes.

Author(s):  
Jingren Wang ◽  
Qingze Zou

In this paper, the problem of rapid probe engagement and withdrawal in atomic force microscopy (AFM) is addressed. Probe engagement to and withdrawal from the sample, respectively, are fundamental steps in all AFM operations, ranging from imaging to nanomanipulation. However, due to the highly nonlinear force-distance relation and the rapid transition between the attractive and the repulsive force dominance, a quick “snap-in” of the probe and excessively large repulsive force during the engagement, and a large adhesive force during the withdrawal are induced, resulting in sample deformation and damage, and measurement errors. Such adverse effects become more severe when the engagement and withdrawal is at high speeds, and the sample is soft (such as the live biological samples). Rapid engagement and withdrawal is needed to achieve high-speed AFM operations, particularly, to capture and interrogate dynamic evolutions of the sample. We propose a learning-based online optimization technique to minimize the probe-sample interaction force in high-speed engagement and withdrawal. Specifically, the desired force and probe position trajectory profile is online designed by using the optimal trajectory design technique, and tracked by using iterative learning control technique. Then the designed force-trajectory profile is online optimized to minimize the engagement force and the adhesive force. The proposed rapid engagement and withdrawal technique is illustrated through experimental implementation on a Polydimethylsiloxane (PDMS) sample.


2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Rina Hirano ◽  
Yasuhiro Arimura ◽  
Tomoya Kujirai ◽  
Mikihiro Shibata ◽  
Aya Okuda ◽  
...  

AbstractH2A.B is an evolutionarily distant histone H2A variant that accumulates on DNA repair sites, DNA replication sites, and actively transcribing regions in genomes. In cells, H2A.B exchanges rapidly in chromatin, but the mechanism has remained enigmatic. In the present study, we found that the H2A.B-H2B dimer incorporated within the nucleosome exchanges with the canonical H2A-H2B dimer without assistance from additional factors, such as histone chaperones and nucleosome remodelers. High-speed atomic force microscopy revealed that the H2A.B nucleosome, but not the canonical H2A nucleosome, transiently forms an intermediate “open conformation”, in which two H2A.B-H2B dimers may be detached from the H3-H4 tetramer and bind to the DNA regions near the entry/exit sites. Mutational analyses revealed that the H2A.B C-terminal region is responsible for the adoption of the open conformation and the H2A.B-H2B exchange in the nucleosome. These findings provide mechanistic insights into the histone exchange of the H2A.B nucleosome.


2006 ◽  
Vol 45 (3B) ◽  
pp. 1897-1903 ◽  
Author(s):  
Toshio Ando ◽  
Takayuki Uchihashi ◽  
Noriyuki Kodera ◽  
Atsushi Miyagi ◽  
Ryo Nakakita ◽  
...  

2011 ◽  
Vol 47 (17) ◽  
pp. 4974 ◽  
Author(s):  
Shigeto Inoue ◽  
Takayuki Uchihashi ◽  
Daisuke Yamamoto ◽  
Toshio Ando

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