scholarly journals A Surface Plasmon Resonance Plastic Optical Fiber Biosensor for the Detection of Pancreatic Amylase in Surgically-Placed Drain Effluent

Sensors ◽  
2021 ◽  
Vol 21 (10) ◽  
pp. 3443
Author(s):  
Laura Pasquardini ◽  
Nunzio Cennamo ◽  
Giuseppe Malleo ◽  
Lia Vanzetti ◽  
Luigi Zeni ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Optical Fiber ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Limit Of Detection ◽  
Optical Biosensor ◽  
Plastic Optical Fiber ◽  
Epithelial Surface ◽  
Cheap Method ◽  
Log Linear

Postoperative pancreatic fistula (POPF), the major driver of morbidity and mortality following pancreatectomy, is caused by an abnormal communication between the pancreatic ductal epithelium and another epithelial surface containing pancreas-derived, enzyme-rich fluid. There is a strong correlation between the amylase content in surgically-placed drains early in the postoperative course and the development of POPF. A simple and cheap method to determine the amylase content from the drain effluent has been eagerly advocated. Here, we developed an amylase optical biosensor, based on a surface plasmon resonance (SPR) plastic optical fiber (POF), metallized with a 60 nm layer of gold and interrogated with white light. The sensor was made specific by coupling it with an anti-amylase antibody. Each surface derivatization step was optimized and studied by XPS, contact angle, and fluorescence. The POF-biosensor was tested for its response to amylase in diluted drain effluents. The volume of sample required was 50 µL and the measurement time was 8 min. The POF-biosensor showed selectivity for amylase, a calibration curve log-linear in the range of 0.8–25.8 U/L and a limit of detection (LOD) of ~0.5 U/L. In preliminary tests, the POF-biosensor allowed for the measurement of the amylase content of diluted surgically-placed drain effluents with an accuracy of >92% with respect to the gold standard. The POF-biosensor allows for reliable measurement and could be implemented to allow for a rapid bedside assessment of amylase value in drains following pancreatectomy.

scholarly journals An Enhanced Plastic Optical Fiber-Based Surface Plasmon Resonance Sensor with a Double-Sided Polished Structure

Sensors ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1516
Author(s):  
Lian Liu ◽  
Shijie Deng ◽  
Jie Zheng ◽  
Libo Yuan ◽  
Hongchang Deng ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Optical Fiber ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Low Cost ◽  
Fiber Axis ◽  
Plastic Optical Fiber ◽  
Au Film ◽  
Spr Sensor ◽  
Resonance Sensor

An enhanced plastic optical fiber (POF)-based surface plasmon resonance (SPR) sensor is proposed by employing a double-sided polished structure. The sensor is fabricated by polishing two sides of the POF symmetrically along with the fiber axis, and a layer of Au film is deposited on each side of the polished region. The SPR can be excited on both polished surfaces with Au film coating, and the number of light reflections will be increased by using this structure. The simulation and experimental results show that the proposed sensor has an enhanced SPR effect. The visibility and full width at half maximum (FWHM) of spectrum can be improved for the high measured refractive index (RI). A sensitivity of 4284.8 nm/RIU is obtained for the double-sided POF-based SPR sensor when the measured liquid RI is 1.42. The proposed SPR sensor is easy fabrication and low cost, which can provide a larger measurement range and action area to the measured samples, and it has potential application prospects in the oil industry and biochemical sensing fields.

Construction aspects of a plastic optical fiber-based surface plasmon resonance biochip

2015 ◽  
Author(s):  
M. F. S. Santiago ◽  
T. B. Silva ◽  
M. H. Mozzini ◽  
I. B. G. Coutinho ◽  
E. S. Medeiros ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Optical Fiber ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Plastic Optical Fiber

scholarly journals Comparison between one layer and bilayer surface Plasmon resonance optical fiber chemical sensor

2020 ◽  
Vol 12 (02) ◽  
pp. 52-60
Author(s):  
Soudad S. Ahmed ◽  
Keyword(s):  
Surface Plasmon Resonance ◽  
Optical Fiber ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Chemical Sensor ◽  
Salt Water ◽  
Water Solution ◽  
Single Layer ◽  
Resonance Wavelength ◽  
Plastic Optical Fiber

Surface Plasmon Resonance (SPR) - based plastic optical fiber has been provided as a sensor to estimating the refractive index and then the concentration of specific chemical samples. Two configurations were suggested for a design. The first was through using a single layer of gold with a thickness of about 40nm deposited on a 10mm portion in the middle of plastic optical fiber. In the second configuration, a bilayer deposited on the fiber. This bilayer consisted of a gold layer with a thickness of about 30 nm and an aluminum layer with a thickness of about 30 nm. Both of these configurations utilized as a chemical sensor. The resonance wavelength for the bilayer-based sensor was higher than that of the single-layer sensor for all studied chemical samples. The highest resonance wavelength was for the salt-water solution with a concentration of 30%. For the salt-water solution with a concentration of 30%, the resonance wavelength with the bilayer-based sensor was 568nm while it was 540nm with the single-layer sensor.

scholarly journals Localized Surface Plasmon Resonance with Five-Branched Gold Nanostars in a Plastic Optical Fiber for Bio-Chemical Sensor Implementation

Sensors ◽  
2013 ◽  
Vol 13 (11) ◽  
pp. 14676-14686 ◽  
Author(s):  
Nunzio Cennamo ◽  
Girolamo D'Agostino ◽  
Alice Donà ◽  
Giacomo Dacarro ◽  
Piersandro Pallavicini ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Optical Fiber ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Chemical Sensor ◽  
Localized Surface Plasmon ◽  
Plastic Optical Fiber ◽  
Gold Nanostars

scholarly journals Fabrication of a Chemical Sensor Based on Surface Plasmon Resonance via Plastic Optical Fiber

2020 ◽  
pp. 765-771
Author(s):  
Ghufran Mohammed Jassam ◽  
Soudad S. Al–Bassam ◽  
Murtadha F. Sultan
Keyword(s):  
Surface Plasmon Resonance ◽  
Optical Fiber ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Figure Of Merit ◽  
Chemical Sensor ◽  
Optical Fiber Sensor ◽  
Numerical Aperture ◽  
Plastic Optical Fiber ◽  
Core Diameter

In this work, a chemical optical fiber sensor based on Surface Plasmon Resonance (SPR) was designed and implemented using plastic optical fiber. The sensor is used for estimating refractive indices and concentrations of various chemical materials (methanol, distilled water, ethanol, kerosene) as well as for evaluating the performance parameters such as sensitivity, signal to noise ratio, resolution and the figure of merit of the fabricated sensor. It was found that the value of the sensitivity of the optical fiber-based SPR sensor, with 40 nm thick and 10 mm long Au metal film of exposed sensing region, was 3μm/RIU, while the SNR was 0.24, the figure of merit was 20, and the resolution was 0.00066. The sort of optical fiber utilized in this work is plastic optical fiber with a core diameter of 980 μm, a fluorinated polymer cladding of 20μm and a numerical aperture of 0.51.

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