scholarly journals Current Development and Applications of Super-Resolution Ultrasound Imaging

Sensors ◽  
2021 ◽  
Vol 21 (7) ◽  
pp. 2417
Author(s):  
Qiyang Chen ◽  
Hyeju Song ◽  
Jaesok Yu ◽  
Kang Kim
Keyword(s):  
Kidney Disease ◽  
Ultrasound Imaging ◽  
Atherosclerotic Plaque ◽  
Super Resolution ◽  
Emerging Technology ◽  
Diffraction Limit ◽  
Disease Models ◽  
Current Development ◽  
Future Directions ◽  
Acoustic Diffraction

Abnormal changes of the microvasculature are reported to be key evidence of the development of several critical diseases, including cancer, progressive kidney disease, and atherosclerotic plaque. Super-resolution ultrasound imaging is an emerging technology that can identify the microvasculature noninvasively, with unprecedented spatial resolution beyond the acoustic diffraction limit. Therefore, it is a promising approach for diagnosing and monitoring the development of diseases. In this review, we introduce current super-resolution ultrasound imaging approaches and their preclinical applications on different animals and disease models. Future directions and challenges to overcome for clinical translations are also discussed.

scholarly journals Acousto optic imaging beyond the acoustic diffraction limit using speckle decorrelation

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Daniel Doktofsky ◽  
Moriya Rosenfeld ◽  
Ori Katz
Keyword(s):  
Scattered Light ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Resolution Limit ◽  
Contrast Imaging ◽  
Optical Contrast ◽  
Ultrasound Modulation ◽  
Wavefront Shaping ◽  
Acoustic Diffraction ◽  
Natural Dynamics

AbstractAcousto-optic imaging (AOI) enables optical-contrast imaging deep inside scattering samples via localized ultrasound modulation of scattered light. However, the resolution in AOI is inherently limited by the ultrasound focus size, prohibiting microscopic investigations. In recent years advances in the field of digital wavefront-shaping allowed the development of novel approaches for overcoming AOI’s acoustic resolution limit. However, these approaches require thousands of wavefront measurements within the sample speckle decorrelation time, limiting their application to static samples. Here, we show that it is possible to surpass the acoustic resolution-limit with a conventional AOI system by exploiting the natural dynamics of speckle decorrelations rather than trying to overcome them. We achieve this by adapting the principles of super-resolution optical fluctuations imaging (SOFI) to AOI. We show that naturally fluctuating optical speckle grains can serve in AOI as the analogues of blinking fluorophores in SOFI, enabling super-resolution by statistical analysis of fluctuating acousto-optic signals.

scholarly journals Super-resolution Ultrasound Imaging of the Renal Microvasculature in Rats with Metabolic syndrome

2020 ◽  
Author(s):  
Stinne Byrholdt Sogaard ◽  
Sofie Bech Andersen ◽  
Iman Taghavi ◽  
Carlos Armando Villagomez Hoyos ◽  
Kristoffer Lindskov Hansen ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Ultrasound Imaging ◽  
Super Resolution ◽  
Renal Microvasculature

scholarly journals MINSTED fluorescence localization and nanoscopy

2021 ◽  
Author(s):  
Michael Weber ◽  
Marcel Leutenegger ◽  
Stefan Stoldt ◽  
Stefan Jakobs ◽  
Tiberiu S. Mihaila ◽  
...  
Keyword(s):  
Standard Deviation ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Stimulated Emission ◽  
Far Field ◽  
Inner Membrane ◽  
Mitochondrial Inner Membrane ◽  
Molecular Scale ◽  
Localization Precision ◽  
Super Resolution Microscopy

AbstractWe introduce MINSTED, a fluorophore localization and super-resolution microscopy concept based on stimulated emission depletion (STED) that provides spatial precision and resolution down to the molecular scale. In MINSTED, the intensity minimum of the STED doughnut, and hence the point of minimal STED, serves as a movable reference coordinate for fluorophore localization. As the STED rate, the background and the required number of fluorescence detections are low compared with most other STED microscopy and localization methods, MINSTED entails substantially less fluorophore bleaching. In our implementation, 200–1,000 detections per fluorophore provide a localization precision of 1–3 nm in standard deviation, which in conjunction with independent single fluorophore switching translates to a ~100-fold improvement in far-field microscopy resolution over the diffraction limit. The performance of MINSTED nanoscopy is demonstrated by imaging the distribution of Mic60 proteins in the mitochondrial inner membrane of human cells.

Sign in / Sign up

Export Citation Format

Share Document