scholarly journals Design of High Performance Scroll Microcoils for Nuclear Magnetic Resonance Spectroscopy of Nanoliter and Subnanoliter Samples

Sensors ◽  
2020 ◽  
Vol 21 (1) ◽  
pp. 170
Author(s):  
Meriam Khelifa ◽  
Denis Mounier ◽  
Nourdin Yaakoubi
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
High Performance ◽  
Signal To Noise Ratio ◽  
Electromagnetic Properties ◽  
Resonance Spectroscopy ◽  
Intrinsic Signal ◽  
Close Proximity ◽  
Nuclear Magnetic

The electromagnetic properties of scroll microcoils are investigated with finite element modelling (FEM) and the design of experiment (DOE) approach. The design of scroll microcoils was optimized for nuclear magnetic resonance (NMR) spectroscopy of nanoliter and subnanoliter sample volumes. The unusual proximity effect favours optimised scroll microcoils with a large number of turns rolled up in close proximity. Scroll microcoils have many advantages over microsolenoids: such as ease of fabrication and better B1-homogeneity for comparable intrinsic signal-to-noise ratio (SNR). Scroll coils are suitable for broadband multinuclei NMR spectroscopy of subnanoliter sample.

scholarly journals Recent Advances in Solid-State Nuclear Magnetic Resonance Spectroscopy

2018 ◽  
Vol 11 (1) ◽  
pp. 485-508 ◽  
Author(s):  
Sharon E. Ashbrook ◽  
John M. Griffin ◽  
Karen E. Johnston
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Solid State ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
Solid State Nmr ◽  
Spectral Lines ◽  
Atomic Scale ◽  
Resonance Spectroscopy ◽  
Diverse Range ◽  
Nuclear Magnetic

The sensitivity of nuclear magnetic resonance (NMR) spectroscopy to the local atomic-scale environment offers great potential for the characterization of a diverse range of solid materials. Despite offering more information than its solution-state counterpart, solid-state NMR has not yet achieved a similar level of recognition, owing to the anisotropic interactions that broaden the spectral lines and hinder the extraction of structural information. Here, we describe the methods available to improve the resolution of solid-state NMR spectra and the continuing research in this area. We also highlight areas of exciting new and future development, including recent interest in combining experiment with theoretical calculations, the rise of a range of polarization transfer techniques that provide significant sensitivity enhancements, and the progress of in situ measurements. We demonstrate the detailed information available when studying dynamic and disordered solids and discuss the future applications of solid-state NMR spectroscopy across the chemical sciences.

scholarly journals Composition dependent transport diffusion in non-ideal mixtures from spatially resolved nuclear magnetic resonance spectroscopy

2018 ◽  
Vol 20 (44) ◽  
pp. 28185-28192 ◽  
Author(s):  
Christian F. Pantoja ◽  
Y. Mauricio Muñoz-Muñoz ◽  
Lorraine Guastar ◽  
Jadran Vrabec ◽  
Julien Wist
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Diffusion Coefficient ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Resonance Spectroscopy ◽  
Spatially Resolved ◽  
Transport Diffusion ◽  
Dependent Transport ◽  
Nuclear Magnetic

Nuclear magnetic resonance (NMR) spectroscopy can also be used for the measurement of the Fick diffusion coefficient.

Use of high-resolution proton nuclear magnetic resonance spectroscopy for rapid multi-component analysis of urine.

1984 ◽  
Vol 30 (3) ◽  
pp. 426-432 ◽  
Author(s):  
J R Bales ◽  
D P Higham ◽  
I Howe ◽  
J K Nicholson ◽  
P J Sadler
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
High Resolution ◽  
1H Nmr ◽  
1H Nmr Spectroscopy ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
Simple Method ◽  
Nuclear Magnetic

Abstract Numerous low-Mr metabolites--including creatinine, citrate, hippurate, glucose, ketone bodies, and various amino acids--have been identified in 400- and 500-MHz proton nuclear magnetic resonance (1H NMR) spectra of intact human urine. The presence of many of these was related to the specific condition of the donors: humans in different physiological states (resting, fasting, or post-exercise) and pathological conditions (e.g., diabetes mellitus, cadmium-induced renal dysfunction). We have also monitored the metabolism of simple nonendogenous compounds (methanol and ethanol) and of acetaminophen. The pH-dependencies of the NMR chemical shifts of some urine components are reported. Our studies show that high-resolution 1H NMR spectroscopy provides a fast, simple method for "fingerprint" identification of urinary compounds. In some cases, analytes can be quantified by standard additions or by comparing integrated peak areas for the metabolites with those for creatinine. Determinations of creatinine by 1H NMR spectroscopy compared well with those by an independent chemical assay based on the Jaffé reaction.

scholarly journals Nuclear Magnetic Resonance Spectroscopy for Medical and Dental Applications: A Comprehensive Review

2019 ◽  
Vol 13 (01) ◽  
pp. 124-128 ◽  
Author(s):  
Komal Zia ◽  
Talal Siddiqui ◽  
Saqib Ali ◽  
Imran Farooq ◽  
Muhammad Sohail Zafar ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
Analytical Techniques ◽  
Resonance Spectroscopy ◽  
Advantages And Disadvantages ◽  
The Past ◽  
Dental Applications ◽  
Insight Into ◽  
Nuclear Magnetic

AbstractNuclear magnetic resonance (NMR) spectroscopy is one of the most significant analytical techniques that has been developed in the past few decades. A broad range of biological and nonbiological applications ranging from an individual cell to organs and tissues has been investigated through NMR. Various aspects of this technique are still under research, and many functions of the NMR are still pending a better understanding and acknowledgment. Therefore, this review is aimed at providing a general overview of the main principles, types of this technique, and the advantages and disadvantages of NMR spectroscopy. In addition, an insight into the current uses of NMR in the field of medicine and dentistry and ongoing developments of NMR spectroscopy for future applications has been discussed.

scholarly journals A Fast Self-Learning Subspace Reconstruction Method for Non-Uniformly Sampled Nuclear Magnetic Resonance Spectroscopy

2020 ◽  
Vol 10 (11) ◽  
pp. 3939 ◽  
Author(s):  
Zhangren Tu ◽  
Huiting Liu ◽  
Jiaying Zhan ◽  
Di Guo
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
The Self ◽  
Resonance Spectroscopy ◽  
Reconstruction Method ◽  
Subspace Method ◽  
High Quality ◽  
Self Learning ◽  
Nuclear Magnetic

Multidimensional nuclear magnetic resonance (NMR) spectroscopy is one of the most crucial detection tools for molecular structure analysis and has been widely used in biomedicine and chemistry. However, the development of NMR spectroscopy is hampered by long data collection time. Non-uniform sampling empowers rapid signal acquisition by collecting a small subset of data. Since the sampling rate is lower than that of the Nyquist sampling ratio, undersampling artifacts arise in reconstructed spectra. To obtain a high-quality spectrum, it is necessary to apply reasonable prior constraints in spectrum reconstruction models. The self-learning subspace method has been shown to possess superior advantages than that of the state-of-the-art low-rank Hankel matrix method when adopting high acceleration in data sampling. However, the self-learning subspace method is time-consuming due to the singular value decomposition in iterations. In this paper, we propose a fast self-learning subspace method to enable fast and high-quality reconstructions. Aided by parallel computing, the experiment results show that the proposed method can reconstruct high-fidelity spectra but spend less than 10% of the time required by the non-parallel self-learning subspace method.

Ascorbic acid, a vitamin, is observed by in vivo13C nuclear magnetic resonance spectroscopy of rat liver

1998 ◽  
Vol 274 (1) ◽  
pp. E65-E71 ◽  
Author(s):  
Ekkehard Küstermann ◽  
Joachim Seelig ◽  
Basil Künnecke
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Ascorbic Acid ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
Rat Liver ◽  
Whole Body ◽  
Resonance Spectroscopy ◽  
C Nmr ◽  
Nuclear Magnetic

The first in vivo detection of a vitamin with nuclear magnetic resonance (NMR) is reported for mammalian liver. Vitamin C, also known as ascorbic acid, was monitored noninvasively in rat liver by “whole body”13C NMR spectroscopy at high field after infusion of [1,2-13C2]glucose into anesthetized rats. Generally, the carbon resonances of ascorbic acid overlap with those of other highly abundant cellular metabolites, thus precluding their observation in situ. This problem was resolved by taking advantage of the13C-13C spin couplings introduced by the two covalently bound13C nuclei in [1,2-13C2]glucose. During glucose metabolism, [5,6-13C2]ascorbic acid was synthesized, which also exhibited characteristic13C homonuclear spin couplings. This feature enabled the spectral discrimination of ascorbic acid from overlapping singlet resonances of other metabolites. Quantitative analysis of the spin-coupling patterns provided an estimate of the turnover rate of hepatic ascorbic acid in vivo (1.9 ± 0.4 nmol ⋅ min−1 ⋅ g−1) and a novel approach toward a better understanding of optimal ascorbic acid requirements in humans. The results obtained in vivo were confirmed with high-resolution proton and13C NMR spectroscopy of liver extracts.

scholarly journals Metabolic Profiling of Saponin-Rich Ophiopogon japonicus Roots Based on 1H NMR and HPTLC Platforms

Planta Medica ◽  
2019 ◽  
Vol 85 (11/12) ◽  
pp. 917-924 ◽  
Author(s):  
Yanhui Ge ◽  
Xiaojia Chen ◽  
Dejan Gođevac ◽  
Paula C. P. Bueno ◽  
Luis F. Salomé Abarca ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Nuclear Magnetic Resonance Spectroscopy ◽  
High Performance ◽  
Resonance Spectroscopy ◽  
Layer Chromatography ◽  
Nuclear Magnetic

AbstractIdeally, metabolomics should deal with all the metabolites that are found within cells and biological systems. The most common technologies for metabolomics include mass spectrometry, and in most cases, hyphenated to chromatographic separations (liquid chromatography- or gas chromatography-mass spectrometry) and nuclear magnetic resonance spectroscopy. However, limitations such as low sensitivity and highly congested spectra in nuclear magnetic resonance spectroscopy and relatively low signal reproducibility in mass spectrometry impede the progression of these techniques from being universal metabolomics tools. These disadvantages are more notorious in studies of certain plant secondary metabolites, such as saponins, which are difficult to analyse, but have a great biological importance in organisms. In this study, high-performance thin-layer chromatography was used as a supplementary tool for metabolomics. A method consisting of coupling 1H nuclear magnetic resonance spectroscopy and high-performance thin-layer chromatography was applied to distinguish between Ophiopogon japonicus roots that were collected from two growth locations and were of different ages. The results allowed the root samples from the two growth locations to be clearly distinguished. The difficulties encountered in the identification of the marker compounds by 1H nuclear magnetic resonance spectroscopy was overcome using high-performance thin-layer chromatography to separate and isolate the compounds. The saponins, ophiojaponin C or ophiopogonin D, were found to be marker metabolites in the root samples and proved to be greatly influenced by plant growth location, but barely by age variation. The procedure used in this study is fully described with the purpose of making a valuable contribution to the quality control of saponin-rich herbal drugs using high-performance thin-layer chromatography as a supplementary analytical tool for metabolomics research.

Uses of Nuclear Magnetic Resonance Spectroscopy Technique in Pharmaceutical Analysis: A Review

2017 ◽  
Vol 8 (02) ◽  
Author(s):  
Imad Hadi Zohra ◽  
Abeer Fauzi Al-Rubaye ◽  
Mohanad Jawad Kadhim
Keyword(s):  
Magnetic Field ◽  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Nuclear Magnetic Resonance Spectroscopy ◽  
Ratio Method ◽  
Resonance Spectroscopy ◽  
Spectroscopic Techniques ◽  
Nuclear Magnetic

Nuclear magnetic resonance (NMR) is a physical phenomenon in which nuclei in a magnetic field absorb and re-emit electromagnetic radiation. Many scientific techniques exploit NMR phenomena to study molecular physics, crystals, and non-crystalline materials through nuclear magnetic resonance spectroscopy. NMR phenomena are also utilized in low-field NMR, NMR spectroscopy and MRI in the Earth's magnetic field (referred to as Earth's field NMR), and in several types of magnetometers. Modern NMR spectroscopy has been emphasizing the application in biomolecular systems and plays an important role in structural biology. NMR spectroscopy is very important to identify a drug or an excipient, evaluate the level of impurities (and to elucidate the structure), observe the course of a decomposition, to evaluate residual solvents, determine the isomeric composition, i.e. the ratio of diastereomers and the enantiomeric excess by means of chiral additive, assess a single drug or drug composition, characterize a polymer mostly being a mixture and used as excipients, identify counter ions (if of organic origin and having protons), characterize an entire formulation, e.g. a tablet. Fundamentals of quantitative NMR spectroscopy NMR spectroscopy can be considered as a primary ratio method of measurement being characterized by the fact that the ratio of substances can be determined directly from the physical context of the measurement without referencing to another substance. NMR has become one of the most powerful and versatile spectroscopic techniques for the analysis of biomacromolecules, allowing characterization of biomacromolecules and their complexes up to 100 kDa. Together with X-ray crystallography.

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