scholarly journals A Surface Plasmon Resonance Biosensor Based on Directly Immobilized Hemoglobin and Myoglobin

Sensors ◽  
2020 ◽  
Vol 20 (19) ◽  
pp. 5572
Author(s):  
Georgi Dyankov ◽  
Ekaterina Borisova ◽  
Evdokia Belina ◽  
Hristo Kisov ◽  
Ivan Angelov ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Structural Integrity ◽  
Complex Structure ◽  
Gold Surface ◽  
Laser Evaporation ◽  
The Matrix ◽  
Direct Immobilization ◽  
The Impact

Immobilization of proteins on a surface plasmon resonance (SPR) transducer is a delicate procedure since loss of protein bioactivity can occur upon contact with the untreated metal surface. Solution to the problem is the use of an immobilization matrix having a complex structure. However, this is at the expense of biosensor selectivity and sensitivity. It has been shown that the matrix-assisted pulsed laser evaporation (MAPLE) method has been successfully applied for direct immobilization (without a built-in matrix) of proteins, preserving their bioactivity. So far, MAPLE deposition has not been performed on a gold surface as required for SPR biosensors. In this paper we study the impact of direct immobilization of heme proteins (hemoglobin (Hb) and myoglobin (Mb)) on their bioactivity. For the purpose, Hb and Mb were directly immobilized by MAPLE technique on a SPR transducer. The bioactivity of the ligands immobilized in the above-mentioned way was assessed by SPR registration of the molecular reactions of various Hb/Mb functional groups. By SPR we studied the reaction between the beta chain of the Hb molecule and glucose, which shows the structural integrity of the immobilized Hb. A supplementary study of films deposited by FTIR and AFM was provided. The experimental facts showed that direct immobilization of an intact molecule was achieved.

The development of a matrix metalloproteinase-1 biosensor based on the surface plasmon resonance imaging technique

2016 ◽  
Vol 8 (34) ◽  
pp. 6428-6435 ◽  
Author(s):  
Anna Tokarzewicz ◽  
Lech Romanowicz ◽  
Iosif Sveklo ◽  
Ewa Gorodkiewicz
Keyword(s):  
Surface Plasmon Resonance ◽  
Matrix Metalloproteinase ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Detection Method ◽  
Imaging Technique ◽  
Surface Plasmon Resonance Imaging ◽  
Matrix Metalloproteinase 1 ◽  
The Matrix

The purpose of this study was to create a new, highly selective biosensor, which uses surface plasmon resonance as a detection method for the quantitative determination of the matrix metalloproteinase-1.

Localized and Propagating Surface Plasmon Resonance Sensors: A Study Using Carbohydrate Binding Protein

2005 ◽  
Vol 876 ◽  
Author(s):  
Chanda Yonzon ◽  
Richard P. Van Duyne
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Specific Binding ◽  
Gold Surface ◽  
Optical Biosensor ◽  
Localized Surface Plasmon ◽  
Carbohydrate Binding ◽  
Binding Studies ◽  
Assembled Monolayers

AbstractThis work encompasses a comparative analysis of the properties of two optical biosensor platforms: (1) the propagating surface plasmon resonance (SPR) sensor based on a planar, thin film gold surface and (2) the localized surface plasmon resonance (LSPR) sensor based on surface confined Ag nanoparticles fabricated by nanosphere lithography. The binding of Concanavalin A (ConA) to mannose-functionalized self-assembled monolayers (SAMs) is chosen to illustrate the similarities and the differences of these sensors. A comprehensive set of non-specific binding studies demonstrate that the single transduction mechanism is due to the specific binding of ConA to the mannose-functionalized surface. Finally, an elementary (2x1) multiplexed version of a LSPR carbohydrate sensing chip to probe the simultaneous binding of ConA to mannose and galactose-functionalized SAMs is also demonstrated.

scholarly journals Surface Plasmon Resonance Enhanced Spontaneous Upconversion and Stimulated Emissions in Glass Ceramics Containing Ba2LaF7 Nanocrystals

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Sai Li ◽  
Wei Lu ◽  
Qihua Yang ◽  
Dacheng Zhou ◽  
Jianbei Qiu ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Glass Ceramic ◽  
Visible Region ◽  
Glass Ceramics ◽  
Random Lasing ◽  
Ag Nps ◽  
The Matrix ◽  
Quenching Method

Glass ceramics containing Yb3+, Er3+ codoped Ba2LaF7 nanocrystals were fabricated via melt quenching method and the subsequent heating treatment. The formation of Ba2LaF7 nanocrystals in the glass ceramics was confirmed by X-ray diffraction (XRD) and transmission electron microscope (TEM). The spontaneous upconversion (UC) emission and the stimulated counterpart as a random lasing action of Er3+, which were related to the characteristic transitions of Er3+ ions, were achieved in the Yb3+, Er3+-doped Ba2LaF7 nanocrystals embedded glass ceramic hybrid. Furthermore, the absorption spectra verified the surface plasmon resonance (SPR) band of Ag, which precipitated from the matrix glasses as Ag nanoparticles (NPs). By incorporating Ag NPs in the glass ceramic hybrid, spontaneous UC emission intensity of Er3+ in visible region was significantly enhanced, while the threshold of the random lasing was decreased from 480 to 350 nJ/cm2.

Grafting Hyaluronic Acid onto Gold Surface to Achieve Low Protein Fouling in Surface Plasmon Resonance Biosensors

2014 ◽  
Vol 6 (15) ◽  
pp. 13034-13042 ◽  
Author(s):  
Xia Liu ◽  
Renliang Huang ◽  
Rongxin Su ◽  
Wei Qi ◽  
Libing Wang ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Gold Surface ◽  
Low Protein ◽  
Protein Fouling
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