scholarly journals A Cost-Effective In Situ Zooplankton Monitoring System Based on Novel Illumination Optimization

Sensors ◽  
2020 ◽  
Vol 20 (12) ◽  
pp. 3471
Author(s):  
Zhiqiang Du ◽  
Chunlei Xia ◽  
Longwen Fu ◽  
Nan Zhang ◽  
Bowei Li ◽  
...  
Keyword(s):  
High Resolution ◽  
Imaging System ◽  
Physiological State ◽  
Cost Effective ◽  
In Situ Monitoring ◽  
Measuring System ◽  
Target Area ◽  
Image Processing Algorithm ◽  
Led Array

A cost-effective and low-power-consumption underwater microscopic imaging system was developed to capture high-resolution zooplankton images in real-time. In this work, dark-field imaging was adopted to reduce backscattering and background noise. To produce an accurate illumination, a novel illumination optimization scheme for the light-emitting diode (LED) array was proposed and applied to design a lighting system for the underwater optical imaging of zooplankton. A multiple objective genetic algorithm was utilized to find the best location of the LED array, which resulted in the specific illumination level and most homogeneous irradiance in the target area. The zooplankton imaging system developed with the optimal configuration of LEDs was tested with Daphnia magna under laboratory conditions. The maximal field of view was 16 mm × 13 mm and the optical resolution was 15 μm. The experimental results showed that the imaging system developed could capture high-resolution and high-definition images of Daphnia. Subsequently, Daphnia individuals were accurately segmented and their geometrical characters were measured by using a classical image processing algorithm. This work provides a cost-effective zooplankton measuring system based on an optimization illumination configuration of an LED array, which has a great potential for minimizing the investment and operating costs associated with long-term in situ monitoring of the physiological state and population conditions of zooplankton.

Development of a measuring system for on-line in situ monitoring of composite materials manufacturing

2016 ◽  
Vol 90 ◽  
pp. 760-770 ◽  
Author(s):  
R. Polanský ◽  
J. Pihera ◽  
J. Komárek ◽  
R. Pavlica ◽  
P. Prosr ◽  
...  
Keyword(s):  
Composite Materials ◽  
In Situ Monitoring ◽  
Measuring System ◽  
On Line

scholarly journals Comparison of high resolution hydrodynamic model outputs with in-situ Argo profiles in the Ionian Sea

2017 ◽  
Vol 18 (1) ◽  
pp. 22 ◽  
Author(s):  
D. KASSIS ◽  
G. KORRES ◽  
A. KONSTANTINIDOU ◽  
L. PERIVOLIOTIS
Keyword(s):  
High Resolution ◽  
Hydrodynamic Model ◽  
Numerical Models ◽  
Regional Scale ◽  
Model Performance ◽  
Temporal Analysis ◽  
In Situ Monitoring ◽  
Ionian Sea ◽  
Nested Models

In-situ monitoring is an essential component for the development of hydrodynamic numerical models. Argo expansion into marginal seas has enabled the advancement of high resolution regional nested models through initialization, assimilation and validation processes. The SANI (Southern Adriatic-Northern Ionian) hydrodynamic model is a regional nested model producing high resolution outputs for the period 2008-2012. For the corresponding time period, 21 free drifting Argo floats recorded Temperature –Salinity (T/S) profiles throughout the region. This study presents the inter-comparison of the two data sets whilst noting interesting aspects of the model performance regarding the representation of the major water masses characteristics of the SANI area. Aside from the inter-comparison in a basin’s scale, a spatio-temporal analysis is also performed. The results indicate an adequate response of the model simulations regarding the basic hydrographic features of the region. Nevertheless, important differences are highlighted mainly in the upper and deep layers of the study area. At a regional scale, inter-annual variability of the model’s performance is observed reflecting the hydrographic changes occurred in the wider area during the study period. Overall, the results present the strong points but also highlight the weaknesses of the model. They also confirm the challenging task of producing high resolution numerical simulations in transitional areas such as the Ionian Sea.

scholarly journals An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites

2016 ◽  
Author(s):  
Peter J. Skene ◽  
Steven Henikoff
Keyword(s):  
Transcription Factor ◽  
High Resolution ◽  
Cost Effective ◽  
Specific Protein ◽  
Micrococcal Nuclease ◽  
Attractive Alternative ◽  
Dna Binding Sites ◽  
Resolution Mapping ◽  
Chromatin Profiling

AbstractWe describe Cleavage Under Targets and Release Using Nuclease (CUT&RUN), a chromatin profiling strategy in which antibody-targeted controlled cleavage by micrococcal nuclease releases specific protein-DNA complexes into the supernatant for paired-end DNA sequencing. Unlike Chromatin Immunoprecipitation (ChIP), which fragments and solubilizes total chromatin, CUT&RUN is performed in situ, allowing for both quantitative high-resolution chromatin mapping and probing of the local chromatin environment. When applied to yeast and human nuclei, CUT&RUN yielded precise transcription factor profiles while avoiding cross-linking and solubilization issues. CUT&RUN is simple to perform and is inherently robust, with extremely low backgrounds requiring only ~1/10th the sequencing depth as ChIP, making CUT&RUN especially cost-effective for transcription factor and chromatin profiling. When used in conjunction with native ChIP-seq and applied to human CTCF, CUT&RUN mapped directional long range contacts at high resolution. We conclude that in situ mapping of protein-DNA interactions by CUT&RUN is an attractive alternative to ChIP-seq.

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