scholarly journals Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements

Sensors ◽  
2020 ◽  
Vol 20 (11) ◽  
pp. 3237
Author(s):  
Jakub Zajíc ◽  
Steven Ripp ◽  
Josef Trögl ◽  
Gabriela Kuncová ◽  
Marie Pospíšilová
Keyword(s):  
Aromatic Hydrocarbon ◽  
Optical Fiber ◽  
Pseudomonas Putida ◽  
Nutrient Medium ◽  
Cell Layer ◽  
Optical Probe ◽  
E Coli ◽  
Toluene Concentration ◽  
Tapered Optical Fiber ◽  
Fiber Element

In this study, we show the repetitive detection of toluene on a tapered optical fiber element (OFE) with an attached layer of Pseudomonas putida TVA8 bioluminescent bioreporters. The bioluminescent cell layer was attached on polished quartz modified with (3-aminopropyl)triethoxysilane (APTES). The repeatability of the preparation of the optical probe and its use was demonstrated with five differently shaped OFEs. The intensity of measured bioluminescence was minimally influenced by the OFE shape, possessing transmittances between 1.41% and 5.00%. OFE probes layered with P. putida TVA8 were used to monitor liquid toluene over a two-week period. It was demonstrated that OFE probes layered with positively induced P. putida TVA8 bioreporters were reliable detectors of toluene. A toluene concentration of 26.5 mg/L was detected after <30 min after immersion of the probe in the toluene solution. Additional experiments also immobilized constitutively bioluminescent cells of E. coli 652T7, on OFEs with polyethyleneimine (PEI). These OFEs were repetitively induced with Lauria-Bertani (LB) nutrient medium. Bioluminescence appeared 15 minutes after immersion of the OFE in LB. A change in pH from 7 to 6 resulted in a decrease in bioluminescence that was not restored following additional nutrient inductions at pH 7. The E. coli 652T7 OFE probe was therefore sensitive to negative influences but could not be repetitively used.

scholarly journals Isolation of a Gene Responsible for the Oxidation oftrans-Anethole topara-Anisaldehyde by Pseudomonas putida JYR-1 and Its Expression in Escherichia coli

2012 ◽  
Vol 78 (15) ◽  
pp. 5238-5246 ◽  
Author(s):  
Dongfei Han ◽  
Ji-Young Ryu ◽  
Robert A. Kanaly ◽  
Hor-Gil Hur
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Putida ◽  
Hypothetical Protein ◽  
Aldehyde Group ◽  
Agrobacterium Vitis ◽  
T7 Promoter ◽  
Content Type ◽  
E Coli ◽  
Cell Extracts ◽  
Isoeugenol Monooxygenase

ABSTRACTA plasmid, pTA163, inEscherichia colicontained an approximately 34-kb gene fragment fromPseudomonas putidaJYR-1 that included the genes responsible for the metabolism oftrans-anethole to protocatechuic acid. Three Tn5-disrupted open reading frame 10 (ORF 10) mutants of plasmid pTA163 lost their abilities to catalyzetrans-anethole. Heterologously expressed ORF 10 (1,047 nucleotides [nt]) under a T7 promoter inE. colicatalyzed oxidative cleavage of a propenyl group oftrans-anethole to an aldehyde group, resulting in the production ofpara-anisaldehyde, and this gene was designatedtao(trans-anetholeoxygenase). The deduced amino acid sequence of TAO had the highest identity (34%) to a hypothetical protein ofAgrobacterium vitisS4 and likely contained a flavin-binding site. Preferred incorporation of an oxygen molecule from water intop-anisaldehyde using18O-labeling experiments indicated stereo preference of TAO for hydrolysis of the epoxide group. Interestingly, unlike the narrow substrate range of isoeugenol monooxygenase fromPseudomonas putidaIE27 andPseudomonas nitroreducensJin1, TAO fromP. putidaJYR-1 catalyzed isoeugenol,O-methyl isoeugenol, and isosafrole, all of which contain the 2-propenyl functional group on the aromatic ring structure. Addition of NAD(P)H to the ultrafiltered cell extracts ofE. coli(pTA163) increased the activity of TAO. Due to the relaxed substrate range of TAO, it may be utilized for the production of various fragrance compounds from plant phenylpropanoids in the future.

scholarly journals In-Process Diameter Measurement Technique for Micro-Optical Fiber with Standing Wave Illumination

2021 ◽  
Author(s):  
Masaki Michihata ◽  
Zhao Zheng ◽  
Daiki Funaiwa ◽  
Sojiro Murakami ◽  
Shotaro Kadoya ◽  
...  
Keyword(s):  
Optical Fiber ◽  
Standing Wave ◽  
Dynamic Range ◽  
Scattered Light ◽  
High Dynamic Range ◽  
Measurement Range ◽  
Diameter Distribution ◽  
Process Measurement ◽  
Air Turbulence ◽  
Tapered Optical Fiber

AbstractIn this paper, we propose an in-process measurement method of the diameter of micro-optical fiber such as a tapered optical fiber. The proposed technique is based on analyzing optically scattered light generated by standing wave illumination. The proposed method is significant in that it requires an only limited measurement range and does not require a high dynamic range sensor. These properties are suitable for in-process measurement. This experiment verified that the proposed method could measure a fiber diameter as stable as ± 0.01 μm under an air turbulence environment. As a result of comparing the measured diameter distribution with those by scanning electron microscopy, it was confirmed that the proposed method has a measurement accuracy better than several hundred nanometers.

scholarly journals Massive photothermal trapping and migration of particles by a tapered optical fiber

2011 ◽  
Vol 19 (18) ◽  
pp. 17065 ◽  
Author(s):  
Hongbao Xin ◽  
Xingmin Li ◽  
Baojun Li
Keyword(s):  
Optical Fiber ◽  
Tapered Optical Fiber ◽  
And Migration

scholarly journals LSPR-based cholesterol biosensor using a tapered optical fiber structure

2019 ◽  
Vol 10 (5) ◽  
pp. 2150 ◽  
Author(s):  
Santosh Kumar ◽  
Brajesh Kumar Kaushik ◽  
Ragini Singh ◽  
Nan-Kuang Chen ◽  
Qing Shan Yang ◽  
...  
Keyword(s):  
Optical Fiber ◽  
Fiber Structure ◽  
Tapered Optical Fiber ◽  
Cholesterol Biosensor

scholarly journals Fabrication and application of a non-contact double-tapered optical fiber tweezers

2017 ◽  
Vol 25 (19) ◽  
pp. 22480 ◽  
Author(s):  
Z.L. Liu ◽  
Y.X. Liu ◽  
Y. Tang ◽  
N. Zhang ◽  
F.P. Wu ◽  
...  
Keyword(s):  
Optical Fiber ◽  
Tapered Optical Fiber

scholarly journals Tapered Optical Fiber Sensor for Label-Free Detection of Biomolecules

Sensors ◽  
2011 ◽  
Vol 11 (4) ◽  
pp. 3780-3790 ◽  
Author(s):  
Ye Tian ◽  
Wenhui Wang ◽  
Nan Wu ◽  
Xiaotian Zou ◽  
Xingwei Wang
Keyword(s):  
Optical Fiber ◽  
Optical Fiber Sensor ◽  
Fiber Sensor ◽  
Label Free ◽  
Label Free Detection ◽  
Tapered Optical Fiber ◽  
Detection Of Biomolecules

Research on the Technique of Optical Trapping Semiconductor Quantum-Dots Based on the Evanescent Field of Tapered Optical Fiber

2015 ◽  
Vol 42 (2) ◽  
pp. 0205006
Author(s):  
商娅娜 Shang Yana ◽  
石庆鹏 Shi Qingpeng ◽  
庞拂飞 Pang Fufei ◽  
倪晴燕 Ni Qingyan ◽  
陈振宜 Chen Zhenyi ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Optical Fiber ◽  
Optical Trapping ◽  
Evanescent Field ◽  
Semiconductor Quantum Dots ◽  
Tapered Optical Fiber

Sensitivity improved tapered optical fiber sensor for dissolved oxygen detection

2020 ◽  
Vol 49 (1) ◽  
pp. 32-44
Author(s):  
Zhuo-Hui Wu ◽  
Qi Wang ◽  
Jia-Xing Duan ◽  
Hong-Zhi Sun ◽  
Qiang Zhao
Keyword(s):  
Optical Fiber ◽  
Dissolved Oxygen ◽  
Optical Fiber Sensor ◽  
Fiber Sensor ◽  
Oxygen Detection ◽  
Tapered Optical Fiber
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