scholarly journals Electrochemical Paper-Based Biosensor Devices for Rapid Detection of Biomarkers

Sensors ◽  
2020 ◽  
Vol 20 (4) ◽  
pp. 967 ◽  
Author(s):  
Manuel Gutiérrez-Capitán ◽  
Antonio Baldi ◽  
César Fernández-Sánchez
Keyword(s):  
Electrochemical Cell ◽  
Point Of Care ◽  
Device Fabrication ◽  
Diagnostic Tools ◽  
Future Prospects ◽  
High Demand ◽  
Paper Substrate ◽  
Speed Advantage ◽  
Detection Of Biomarkers

In healthcare, new diagnostic tools that help in the diagnosis, prognosis, and monitoring of diseases rapidly and accurately are in high demand. For in-situ measurement of disease or infection biomarkers, point-of-care devices provide a dramatic speed advantage over conventional techniques, thus aiding clinicians in decision-making. During the last decade, paper-based analytical devices, combining paper substrates and electrochemical detection components, have emerged as important point-of-need diagnostic tools. This review highlights significant works on this topic over the last five years, from 2015 to 2019. The most relevant articles published in 2018 and 2019 are examined in detail, focusing on device fabrication techniques and materials applied to the production of paper fluidic and electrochemical cell architectures as well as on the final device assembly. Two main approaches were identified, that are, on one hand, those ones where the fabrication of the electrochemical cell is done on the paper substrate, where the fluidic structures are also defined, and, on the other hand, the fabrication of those ones where the electrochemical cell and liquid-driving paper component are defined on different substrates and then heterogeneously assembled. The main limitations of the current technologies are outlined and an outlook on the current technology status and future prospects is given.

Fabrication Technologies and Applications of Graphene-based co-polymeric Nano-composites: its challenges and Future works

2019 ◽  
Vol 09 ◽  
Author(s):  
Pratik Chhapia ◽  
Harshad Patel
Keyword(s):  
Review Paper ◽  
Polymeric Coating ◽  
Nano Composites ◽  
Future Prospects ◽  
Electrical And Optical Properties ◽  
In Situ Techniques ◽  
Surface Areas ◽  
Ink Jet ◽  
Enhanced Conductivity

: Graphene based co-polymeric Nano-composites explored and trending in various applications as ascribing to its enhanced conductivity and controlled modification with wide specific surface areas. With the number of advantages of co-polymeric coating on Graphene or Graphene sheets and their derivatives, Graphene based co-polymeric Nano-composites fabricated by various techniques (deposition, ink jet, electro spinning, spin coating, in-situ techniques, etc.) and different conducting co-polymers show its exceptional chemical, mechanical, electrical and optical properties. Therefore, in the today’s world with greater quantities of various properties of co-polymer with Graphene based Nano-composites with enhanced stability, selectivity and sensitivity have been formed. In this review paper, we have particularly focused on recent advancing in fabrication of different technologies with the help of Graphene based co-polymeric Nano-composites and its various trending and future applications. Finally, on the personal standpoint; the key challenges of Graphene based co-polymeric Nano-composites are mentioned in hope to shed a light on their potential future prospects.

A Thread/Fabric-Based Band as A Flexible and Wearable Microfluidic Device for Sweat Sensing and Monitoring

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Zhiqi Zhao ◽  
Qiujin Li ◽  
Linna Chen ◽  
Yu Zhao ◽  
Jixian Gong ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Point Of Care ◽  
Monitoring Systems ◽  
Biological Analytes

Flexible biosensors for monitoring systems have emerged as a promising portable diagnostics platform due to their potential for in situ point-of-care (POC) analytic devices. Assessment of biological analytes in sweat...

scholarly journals Flow Control in Porous Media: From Numerical Analysis to Quantitative μPAD for Ionic Strength Measurements

Sensors ◽  
2021 ◽  
Vol 21 (10) ◽  
pp. 3328
Author(s):  
Pouya Mehrdel ◽  
Hamid Khosravi ◽  
Shadi Karimi ◽  
Joan Antoni López Martínez ◽  
Jasmina Casals-Terré
Keyword(s):  
Porous Media ◽  
Limit Of Detection ◽  
Fluid Dynamic ◽  
Porous Membrane ◽  
External Pressure ◽  
Paper Substrate ◽  
Sensor Performance ◽  
Food Applications ◽  
Substrate Nature

Microfluidic paper-based analytical devices (µPADs) are a promising technology to enable accurate and quantitative in situ assays. Paper’s inherent hydrophilicity drives the fluids without the need for external pressure sources. However, controlling the flow in the porous medium has remained a challenge. This study addresses this problem from the nature of the paper substrate and its design. A computational fluid dynamic model has been developed, which couples the characteristics of the porous media (fiber length, fiber diameter and porosity) to the fluidic performance of the diffusion-based µPAD sensor. The numerical results showed that for a given porous membrane, the diffusion, and therefore the sensor performance is affected not only by the substrate nature but also by the inlets’ orientation. Given a porous substrate, the optimum performance is achieved by the lowest inlets’ angle. A diffusion-based self-referencing colorimetric sensor was built and validated according to the design. The device is able to quantify the hydronium concentration in wines by comparison to 0.1–1.0 M tartaric acid solutions with a 41.3 mM limit of detection. This research showed that by proper adjustments even the simplest µPADs can be used in quantitative assays for agri-food applications.

The AMPIX electrochemical cell: a versatile apparatus forin situX-ray scattering and spectroscopic measurements

2012 ◽  
Vol 45 (6) ◽  
pp. 1261-1269 ◽  
Author(s):  
Olaf J. Borkiewicz ◽  
Badri Shyam ◽  
Kamila M. Wiaderek ◽  
Charles Kurtz ◽  
Peter J. Chupas ◽  
...  
Keyword(s):  
Electrochemical Cell ◽  
Function Analysis ◽  
Kinetic Studies ◽  
Spectroscopic Measurements ◽  
Battery Materials ◽  
X Ray ◽  
Complex Processes ◽  
Electrochemical Cycling ◽  
Ray Scattering

This article presents a versatile easy-to-use electrochemical cell suitable forin operando,in situmeasurements of battery materials during electrochemical cycling using a variety of X-ray techniques. Argonne's multi-purposein situX-ray (AMPIX) cell provides reliable electrochemical cycling over extended periods owing to the uniform stack pressure applied by rigid X-ray windows and the formation of a high-fidelity hermetic seal. The suitability of the AMPIX cell for a broad range of synchrotron-based X-ray scattering and spectroscopic measurements has been demonstrated with studies at eight Advanced Photon Source beamlines to date. Compatible techniques include pair distribution function analysis, high-resolution powder diffraction, small-angle scattering and X-ray absorption spectroscopy. These techniques probe a broad range of electronic, structural and morphological features relevant to battery materials. The AMPIX cell enables experiments providing greater insight into the complex processes that occur in operating batteries by allowing the electrochemical reactions to be probed at fine reaction intervals with greater consistency (within the charge–discharge cycle and between different methodologies) with potential for new time-dependent kinetic studies or studies of transient species. Representative X-ray and electrochemical data to demonstrate the functionality of the AMPIX cell are presented.

scholarly journals Comparative Analysis of the Euroimmun CXCL13 Enzyme-Linked Immunosorbent Assay and the ReaScan Lateral Flow Immunoassay for Diagnosis of Lyme Neuroborreliosis

2020 ◽  
Vol 58 (9) ◽  
Author(s):  
Katharina Ziegler ◽  
Anca Rath ◽  
Christoph Schoerner ◽  
Renate Meyer ◽  
Thomas Bertsch ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Roc Curve ◽  
Point Of Care ◽  
Lateral Flow ◽  
Lyme Neuroborreliosis ◽  
Diagnostic Tools ◽  
Lateral Flow Immunoassay ◽  
Enzyme Linked Immunosorbent Assay ◽  
European Federation ◽  
Point Of Care Test

ABSTRACT Diagnosis of Lyme neuroborreliosis (LNB) is challenging, as long as Borrelia-specific intrathecal antibodies are not yet detectable. The chemokine CXCL13 is elevated in the cerebrospinal fluid (CSF) of LNB patients. Here, we compared the performances of the Euroimmun CXCL13 enzyme-linked immunosorbent assay (CXCL13 ELISA) and the ReaScan CXCL13 lateral flow immunoassay (CXCL13 LFA), a rapid point-of-care test, to support the diagnosis of LNB. In a dual-center case-control study, CSF samples from 90 patients (34 with definite LNB, 10 with possible LNB, and 46 with other central nervous system [CNS] diseases [non-LNB group]) were analyzed with the CXCL13 ELISA and the CXCL13 LFA. Classification of patients followed the European Federation of Neurological Societies (EFNS) guidelines on LNB. The CXCL13 ELISA detected elevated CXCL13 levels in all patients with definite LNB (median, 1,409 pg/ml) compared to the non-LNB controls (median, 20.7 pg/ml; P < 0.0001), with a sensitivity of 100% and a specificity of 84.8% (cutoff value, 78.6 pg/ml; area under the receiver operating characteristic [ROC] curve, 0.93). Similarly, the CXCL13 LFA yielded elevated CXCL13 levels in 31 patients with definite LNB (median arbitrary value, 223.5) compared to the non-LNB control patients (median arbitrary value, 0; P < 0.0001) and had a sensitivity and specificity of 91.2% and 93.5%, respectively (cutoff arbitrary value, 22.5; area under the ROC curve, 0.94). The correlation between the CXCL13 levels obtained by ELISA and LFA was strong (Spearman correlation coefficient r = 0.89; P < 0.0001). The CXCL13 ELISA and the CXCL13 LFA are comparable diagnostic tools for the detection of CXCL13 in the CSF of patients with definite LNB. The advantage of the CXCL13 LFA is the shorter time to result.

Simulation at the point of care: Reduced-cost, in situ training via a mobile cart

2009 ◽  
Vol 10 (2) ◽  
pp. 176-181 ◽  
Author(s):  
Peter H. Weinstock ◽  
Liana J. Kappus ◽  
Alexander Garden ◽  
Jeffrey P. Burns
Keyword(s):  
Point Of Care ◽  
In Situ Training

scholarly journals In-Situ Diagnostics at High Pressures: Ellipsometric and Rheed Studies of the Growth of Yba2Cu3O7

1997 ◽  
Vol 502 ◽  
Author(s):  
Dave H. A. Blank ◽  
Horst Rogalla
Keyword(s):  
Thin Films ◽  
High Pressures ◽  
High Vacuum ◽  
Pulsed Laser ◽  
Complex Oxide ◽  
High Energy ◽  
Diagnostic Tools ◽  
Heteroepitaxial Growth ◽  
Deposition Parameters

ABSTRACTPulsed Laser and Sputter Deposition are used for the fabrication of complex oxide thin films at relatively high oxygen pressures (up to 0.5 mBar). This high pressure hampers the application of a number of in-situ diagnostic tools. One of the exceptions is ellipsometry. Using this technique we studied in-situ the growth of off-axis sputtered Yba2Cu3O6+x thin films on (001) SrTiO3 as a function of the deposition parameters. Furthermore, the oxidation process from O(6) to O(7) has been studied by performing spectroscopic ellipsometry during isobaric cooling procedures.Another suitable in-situ monitoring technique for the growth of thin films is Reflection High Energy Electron Diffraction (RHEED). In general this is a (high) vacuum technique. Here, we present an RHEED-system in which we can observe clear diffraction patterns up to a deposition pressure of 0.5 mBar. The system has been used for in-situ monitoring of the heteroepitaxial growth of YBa2Cu3 06+x on SrTiO3 by pulsed laser deposition.

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