scholarly journals Quantification of Neuropeptide Y with Picomolar Sensitivity Enabled by Guided-Mode Resonance Biosensors

Sensors ◽  
2019 ◽  
Vol 20 (1) ◽  
pp. 126 ◽  
Author(s):  
Mohammad G. Abdallah ◽  
Joseph A. Buchanan-Vega ◽  
Kyu J. Lee ◽  
Brett R. Wenner ◽  
Jeffery W. Allen ◽  
...  
Keyword(s):  
Neuropeptide Y ◽  
Near Infrared ◽  
Single Point ◽  
High Sensitivity ◽  
Quantitative Detection ◽  
Label Free ◽  
Sensor Surface ◽  
Detection Range ◽  
Guided Mode ◽  
Guided Mode Resonance

Assessing levels of neuropeptide Y (NPY) in the human body has many medical uses. Accordingly, we report the quantitative detection of NPY biomarkers applying guided-mode resonance (GMR) biosensor methodology. The label-free sensor operates in the near-infrared spectral region exhibiting distinctive resonance signatures. The interaction of NPY with bioselective molecules on the sensor surface causes spectral shifts that directly identify the binding event without additional processing. In the experiments described here, NPY antibodies are attached to the sensor surface to impart specificity during operation. For the low concentrations of NPY of interest, we apply a sandwich NPY assay in which the sensor-linked anti-NPY molecule binds with NPY that subsequently binds with anti-NPY to close the sandwich. The sandwich assay achieves a detection limit of ~0.1 pM NPY. The photonic sensor methodology applied here enables expeditious high-throughput data acquisition with high sensitivity and specificity. The entire bioreaction is recorded as a function of time, in contrast to label-based methods with single-point detection. The convenient methodology and results reported are significant, as the NPY detection range of 0.1–10 pM demonstrated is useful in important medical circumstances.

scholarly journals A Compact Detection Platform Based on Gradient Guided-Mode Resonance for Colorimetric and Fluorescence Liquid Assay Detection

Sensors ◽  
2021 ◽  
Vol 21 (8) ◽  
pp. 2797
Author(s):  
Jing-Jhong Gao ◽  
Ching-Wei Chiu ◽  
Kuo-Hsing Wen ◽  
Cheng-Sheng Huang
Keyword(s):  
Limit Of Detection ◽  
Detection System ◽  
Fluorescence Emission ◽  
Assay Sensitivity ◽  
Detection Range ◽  
Current Form ◽  
Guided Mode ◽  
Spectral Detection ◽  
Guided Mode Resonance ◽  
Colorimetric Assays

This paper presents a compact spectral detection system for common fluorescent and colorimetric assays. This system includes a gradient grating period guided-mode resonance (GGP-GMR) filter and charge-coupled device. In its current form, the GGP-GMR filter, which has a size of less than 2.5 mm, can achieve a spectral detection range of 500–700 nm. Through the direct measurement of the fluorescence emission, the proposed system was demonstrated to detect both the peak wavelength and its corresponding intensity. One fluorescent assay (albumin) and two colorimetric assays (albumin and creatinine) were performed to demonstrate the practical application of the proposed system for quantifying common liquid assays. The results of our system exhibited suitable agreement with those of a commercial spectrometer in terms of the assay sensitivity and limit of detection (LOD). With the proposed system, the fluorescent albumin, colorimetric albumin, and colorimetric creatinine assays achieved LODs of 40.99 and 398 and 25.49 mg/L, respectively. For a wide selection of biomolecules in point-of-care applications, the spectral detection range achieved by the GGP-GMR filter can be further extended and the simple and compact optical path configuration can be integrated with a lab-on-a-chip system.

Fabrication of Precise Asymmetric Nanoshells Array with Nanogaps for a Label-Free Immunoassay Based on NIR-Light Responsive LSPR

2012 ◽  
Vol 523-524 ◽  
pp. 680-685
Author(s):  
Shuhei Uchida ◽  
Kazuya Yamamura ◽  
Nobuyuki Zettsu
Keyword(s):  
Near Infrared ◽  
Atmospheric Pressure Plasma ◽  
High Sensitivity ◽  
Medical Diagnostics ◽  
Localized Surface Plasmon ◽  
Label Free ◽  
Fabrication Technique ◽  
Alternative Structures ◽  
Wide Range ◽  
Surrounding Refractive Index

Localized surface plasmon resonance (LSPR) based sensors are a well established technology utilized for label-free biochemical sensing in immunoassay, medical diagnostics and environmental monitoring. The understanding of asymmetric metal nanoparticles, new object for complex, coupled plasmon systems providing localized significantly enhanced E-field, is central to a wide range of novel applications and processes in science of higher sensitive sensing systems. However, few methods are available for actual characterization of such nanostructures at the single particle level. Here we propose a precise and large sized scale fabrication technique for asymmetric nanoshells array with nanogaps of several tens of nanometers for LSPR sensor through atmospheric pressure plasma etching processes. A nanoshell was simply constructed by laminating thin Au films on periodic isolated polymer nanoparticles template. This nanoshells array was expected to exhibit specific near-infrared plasmonic properties. When measuring the sensitivity, nanoshells array exhibited a high sensitivity to changes of surrounding refractive index and showed a higher sensor figure of merit than the alternative structures. This indicated that the enhanced plasmon E-field in the asymmetric nanostructures improved sensor performance. Our fabrication technique and the optical properties of the arrays will provide useful information for developing new plasmonic applications.

scholarly journals Compact Inner-Wall Grating Slot Microring Resonator for Label-Free Sensing

Sensors ◽  
2019 ◽  
Vol 19 (22) ◽  
pp. 5038 ◽  
Author(s):  
Hongjun Gu ◽  
He Gong ◽  
Chunxue Wang ◽  
Xiaoqiang Sun ◽  
Xibin Wang ◽  
...  
Keyword(s):  
Refractive Index ◽  
Sodium Chloride ◽  
High Sensitivity ◽  
Microring Resonator ◽  
Silicon On Insulator ◽  
Label Free ◽  
Detection Range ◽  
Sodium Chloride Solutions ◽  
Sensing Application ◽  
Index Unit

In this paper, we present and analyze a compact inner-wall grating slot microring resonator (IG-SMRR) with the footprint of less than 13 μm × 13 μm on the silicon-on-insulator (SOI) platform for label-free sensing, which comprises a slot microring resonator (SMRR) and inner-wall grating (IG). Its detection range is significantly enhanced without the limitation of the free spectral region (FSR) owing to the combination of SMRR and IG. The IG-SMRR has an ultra-large quasi-FSR of 84.5 nm as the detection range, and enlarged factor is up to over 3 compared with the conventional SMRR. The concentration sensitivities of sodium chloride solutions and D-glucose solutions are 996.91 pm/% and 968.05 pm/%, respectively, and the corresponding refractive index (RI) sensitivities are 559.5 nm/RIU (refractive index unit) and 558.3 nm/RIU, respectively. The investigation on the combination of SMRR and IG is a valuable exploration of label-free sensing application for ultra-large detection range and ultra-high sensitivity in future.

Sensitive metal layer-assisted guided-mode resonance SU8 nanopillar array for label-free optical biosensing

2016 ◽  
Vol 226 ◽  
pp. 204-210 ◽  
Author(s):  
Víctor Canalejas-Tejero ◽  
Ana López ◽  
Rafael Casquel ◽  
Miguel Holgado ◽  
Carlos Angulo Barrios
Keyword(s):  
Metal Layer ◽  
Label Free ◽  
Optical Biosensing ◽  
Guided Mode ◽  
Guided Mode Resonance

Detection of Sodium Ions by SPR Sensor Using Modified Self-Assembled Calix[4]Arene Deravative Monolayer

2015 ◽  
Vol 799-800 ◽  
pp. 915-918
Author(s):  
M. Benounis ◽  
Nicole Jaffrezic ◽  
Isabelle Bonnamour ◽  
Nadhir Messai
Keyword(s):  
Impedance Spectroscopy ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Gold Surface ◽  
Sodium Ions ◽  
Sensor Surface ◽  
Detection Range ◽  
Spr Sensor ◽  
Linear Detection ◽  
Influence Of Ph

A new SPR sensor surface based onself-assembled-Calix [4] arene-derivative-monolayer was proposed for the detection of sodium in water. The immobisation of claixarene onto the gold surface was confirmed by impedance spectroscopy (EIS). Three alkaliions were used K+, Na+and Ca2+and the influence of pH on ions detection was studied and optimized. The Calix [4] arene-gold SPR sensor developed was characterized by low limit of detection (LOD) for about 10-10M, high sensitivity and wide linear detection range between 10-6M and 10-14M.

scholarly journals A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging

Sensors ◽  
2020 ◽  
Vol 20 (17) ◽  
pp. 4768 ◽  
Author(s):  
Zongcheng Wang ◽  
Huihuang Zheng ◽  
Chengliang Zhang ◽  
Dongfang Tang ◽  
Qiyao Wu ◽  
...  
Keyword(s):  
Fluorescent Sensor ◽  
High Sensitivity ◽  
Living Cells ◽  
Fluorescent Sensors ◽  
Quantitative Detection ◽  
Fast Reaction ◽  
Detection Range ◽  
Long Wavelength ◽  
Turn On

The content of selenocysteine in cells has an important effect on a variety of human diseases, and the detection of selenocysteine by fluorescent sensors in vivo has shown many advantages. In order to further develop fast-reaction-time, good-selectivity, and high-sensitivity long-wavelength selenocysteine fluorescent sensors, we designed and synthesized the compound YZ-A4 as a turn-on fluorescent sensor to detect the content of selenocysteine. The quantitative detection range of the sensor YZ-A4 to selenocysteine was from 0 to 32 μM, and the detection limit was as low as 11.2 nM. The sensor displayed a rapid turn-on response, good selectivity, and high sensitivity to selenocysteine. Finally, we have demonstrated that YZ-A4 could be used for fluorescence imaging of selenocysteine in living cells.

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