scholarly journals A Novel Thiophene-Based Fluorescent Chemosensor for the Detection of Zn2+ and CN−: Imaging Applications in Live Cells and Zebrafish

Sensors ◽  
2019 ◽  
Vol 19 (24) ◽  
pp. 5458 ◽  
Author(s):  
Min Seon Kim ◽  
Dongju Yun ◽  
Ju Byeong Chae ◽  
Haeri So ◽  
Hyojin Lee ◽  
...  
Keyword(s):  
Hela Cells ◽  
Fluorescence Enhancement ◽  
Limit Of Detection ◽  
Zinc Ion ◽  
World Health ◽  
Live Cells ◽  
Real Samples ◽  
Turn On ◽  
Health Organization ◽  
Nmr Titrations

A novel fluorescent turn-on chemosensor DHADC ((E)-3-((4-(diethylamino)-2-hydroxybenzylidene)amino)-2,3-dihydrothiophene-2-carboxamide) has been developed and used to detect Zn2+ and CN−. Compound DHADC displayed a notable fluorescence increase with Zn2+. The limit of detection (2.55 ± 0.05 μM) for zinc ion was far below the standard (76 μM) of the WHO (World Health Organization). In particular, compound DHADC could be applied to determine Zn2+ in real samples, and to image Zn2+ in both HeLa cells and zebrafish. Additionally, DHADC could detect CN− through a fluorescence enhancement with little inhibition with the existence of other types of anions. The detection processes of compound DHADC for Zn2+ and CN− were demonstrated with various analytical methods like Job plots, 1H NMR titrations, and ESI-Mass analyses.

scholarly journals A Chelation-Enhanced Fluorescence Assay Using Thiourea Capped Carbonaceous Fluorescent Nanoparticles For As (III) Detection In Water Samples

2021 ◽  
Author(s):  
Susan Mohammadi ◽  
Somayeh Mohammadi ◽  
Abdollah Salimi ◽  
Rezgar Ahmadi
Keyword(s):  
Water Samples ◽  
Limit Of Detection ◽  
Threshold Value ◽  
Water Soluble ◽  
World Health ◽  
Fluorescent Nanoparticles ◽  
Detection Range ◽  
Turn On ◽  
Efficient Detection ◽  
Health Organization

Abstract Herein, we designed a sensitive and selective “Turn-On” fluorescence nanosensor using water-soluble carbonaceous fluorescent nanomaterials (CFNs) functionalized with thiourea (CFNs-thiourea) for efficient detection of trace amounts of arsenic (III) in aqueous samples. The CFNs and CFNs-Thiourea were characterized by transmission electron microscopy (TEM), UV–Visible spectroscopy (UV-vis) and Fourier transformed infrared spectroscopy (FTIR). The emission peak intensity of proposed nanosensor at 425 nm was gradually enhanced on arsenite addition in wide detection range (3.3–828.5 µg L-1) attributed to the binding of arsenite species with sulfur groups of CFNs-thiourea. The limit of detection (LOD) was 0.48 µg L-1 being much lower than the World Health Organization (WHO) recommended threshold value of 10 µg L-1. Furthermore, the as prepared thiourea -CFNs exhibited an superb selectivity for As (III) compared various cations and anions such as; NO3−, NO2−, F−, Ni2+, Fe3+, Cu2+, Ca2+, Mg2+, Zn2+, Fe2+, Hg2+, Pb2+, F-, Cl-, Mn2+, Cr3+, Co2+, Cd2+, Bi3+, Al3+ and As (V) at 100 folds concentration of As (III). The turn on fluorescence nanosensor was successfully exploited for quantification of arsenic in spiked water samples with acceptable efficiencies.

scholarly journals Water Quality of the Trstionica River (Bosnia and Herzegovina)

2020 ◽  
Vol 69 (7-8) ◽  
pp. 371-376
Author(s):  
Šuhreta Delibašić ◽  
Jasna Huremović ◽  
Sabina Žero ◽  
Sabina Gojak-Salimović
Keyword(s):  
Water Quality ◽  
Bosnia And Herzegovina ◽  
Limit Of Detection ◽  
Weather Conditions ◽  
Ion Exchange Resin ◽  
World Health ◽  
Mean Values ◽  
Significant Difference ◽  
Health Organization

The present study was conducted to investigate the water quality of the Trstionica River, Bosnia and Herzegovina. The physicochemical properties (temperature, pH, conductivity, total solids after evaporation at 105 °C), content of metals (calcium (Ca), cadmium (Cd), chromium (Cr), copper (Cu), iron (Fe), magnesium (Mg), manganese (Mn), sodium (Na), nickel (Ni), lead (Pb) and zinc (Zn)), and anions (chloride (Cl–), and phosphate (PO43–)) were determined in water samples collected at seven locations during two sampling periods: unstable weather conditions (precipitation), and stable weather conditions (without precipitation). There was a significant difference in the content of individual parameters in the river water depending on the sampling time. For determination of metals concentrations, which were below the limit of detection, a preconcentration method using an ion-exchange resin was applied. The metals concentrations during the rainy day were in the order Ca > Mg > Na > Fe > Cu > Zn > Pb > Mn with mean values of 343, 6.03, 1.94, 0.18, 0.20, 0.03, 0.02, 0.01 mg dm–3, respectively, and during stable weather conditions: Ca > Mg > Na > Cu > Fe > Mn > Zn with mean values of 288, 7.62, 2.38, 0.11, 0.10, 0.01, 0.01 mg dm–3, respectively. Cd, Cr, and Ni concentrations were below limit of detection in both cases. Obtained values were compared with World Health Organization (WHO) regulations. The results showed that the Trstionica River in the investigated part of the stream meets most of the parameters required by the regulations. The correlation between analysed parameters was assessed, as well. Based on the calculated water quality index values, the water of Trstionica River falls into the category of excellent water.

scholarly journals A 7-Hydroxybenzoxazinone-Containing Fluorescence Turn-On Probe for Biothiols and Its Bioimaging Applications

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3102 ◽  
Author(s):  
Bin Li ◽  
Datong Zhang ◽  
Ruibing An ◽  
Yaling Zhu
Keyword(s):  
Amino Acids ◽  
Detection Limit ◽  
High Resistance ◽  
Hela Cells ◽  
Fluorescence Enhancement ◽  
Stokes Shift ◽  
Potential Utility ◽  
Strong Fluorescence ◽  
Turn On ◽  
Fluorescence Turn On

In this work, a novel 7-hydroxybenzoxazinone-based fluorescent probe (PBD) for the selective sensing of biothiols is reported. Upon treatment with biothiols, PBD shows a strong fluorescence enhancement (up to 70-fold) and a large Stokes shift (155 nm). Meanwhile, this probe exhibits high resistance to interference from other amino acids and competing species. PBD features good linearity ranges with a low detection limit of 14.5 nM for glutathione (GSH), 17.5 nM for cysteine (Cys), and 80.0 nM for homocysteine (Hcy), respectively. Finally, the potential utility of this probe for biothiol sensing in living HeLa cells is demonstrated.

A novel turn-on fluorescent probe for selective sensing and imaging of glutathione in live cells and organisms

The Analyst ◽  
2019 ◽  
Vol 144 (10) ◽  
pp. 3260-3266 ◽  
Author(s):  
Xiaodong Zhang ◽  
Ziqian Wang ◽  
Zongwei Guo ◽  
Nianzhe He ◽  
Peng Liu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Hela Cells ◽  
K562 Cells ◽  
Live Cells ◽  
Zebrafish Embryos ◽  
Turn On ◽  
Imatinib Resistant

A novel 1-oxo-1H-phenalene-2,3-dicarbonitrile (OPD)-based fluorescent probe was developed to sense and image GSH in HeLa cells, different imatinib-resistant K562 cells, D. magna and zebrafish embryos.

scholarly journals Guidance for Studies Evaluating the Accuracy of Rapid Tuberculosis Drug-Susceptibility Tests

2019 ◽  
Vol 220 (Supplement_3) ◽  
pp. S126-S135 ◽  
Author(s):  
Sophia B Georghiou ◽  
Samuel G Schumacher ◽  
Timothy C Rodwell ◽  
Rebecca E Colman ◽  
Paolo Miotto ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Evaluation Studies ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
World Health ◽  
Policy Recommendations ◽  
Operational Characteristics ◽  
Susceptibility Tests ◽  
Health Organization ◽  
Intended Use

Abstract The development and implementation of rapid molecular diagnostics for tuberculosis (TB) drug-susceptibility testing is critical to inform treatment of patients and to prevent the emergence and spread of resistance. Optimal trial planning for existing tests and those in development will be critical to rapidly gather the evidence necessary to inform World Health Organization review and to support potential policy recommendations. The evidence necessary includes an assessment of the performance for TB and resistance detection as well as an assessment of the operational characteristics of these platforms. The performance assessment should include analytical studies to confirm the limit of detection and assay ability to detect mutations conferring resistance across globally representative strains. The analytical evaluation is typically followed by multisite clinical evaluation studies to confirm diagnostic performance in sites and populations of intended use. This paper summarizes the considerations for the design of these analytical and clinical studies.

scholarly journals Coplanarity driven fluorescence turn-on sensor for chromium(iii) and its application for bio-imaging

2018 ◽  
Vol 17 (2) ◽  
pp. 239-244 ◽  
Author(s):  
G. Balamurugan ◽  
S. Velmathi
Keyword(s):  
Hela Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescence Enhancement ◽  
Live Cell ◽  
Turn On ◽  
Bio Imaging ◽  
Heteroaromatic Aldehyde ◽  
Fluorescence Turn On

The sensing behaviour of benzimidazole and thiozole derivatives from heteroaromatic aldehyde was studied towards various cations and anions. S1 showed selectivity towards Cr3+ with fluorescence enhancement and live cell imaging of Hela cells was successfully demonstrated.

Constructing a FRET-based molecular chemodosimeter for cysteine over homocysteine and glutathione by naphthalimide and phenazine derivatives

The Analyst ◽  
2015 ◽  
Vol 140 (1) ◽  
pp. 182-189 ◽  
Author(s):  
Lin Yang ◽  
Weisong Qu ◽  
Xiao Zhang ◽  
Yandi Hang ◽  
Jianli Hua
Keyword(s):  
Hela Cells ◽  
Fluorescence Enhancement ◽  
Turn On

A chemodosimeter PHSN achieved turn-on fluorescence enhancement at 540 nm and discrimination of cysteine from homocysteine and glutathione in Hela cells.

scholarly journals Analytical evaluation of thirty-two SARS-CoV-2 lateral flow antigen tests demonstrates sensitivity remains with the SARS-CoV-2 Gamma lineage

2021 ◽  
Author(s):  
Konstantina Kontogianni ◽  
Daisy Bengey ◽  
Dominic Wooding ◽  
Kate Buist ◽  
Caitlin Greenland-Bews ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Lateral Flow ◽  
World Health ◽  
Genome Copy ◽  
Copy Numbers ◽  
Alpha Variant ◽  
And Performance ◽  
Antigen Tests ◽  
Health Organization ◽  
Lateral Flow Tests

AbstractThe limit of detection (LOD) of thirty-two antigen lateral flow tests (Ag-RDT) were evaluated with the SARS-CoV-2 Gamma variant. Twenty-eight of thirty-two Ag-RDTs exceeded the World Health Organization criteria of an LOD of 1.0×106 genome copy numbers/ml and performance was equivalent as with the 2020 B.1 lineage and Alpha variant.

scholarly journals A 2-Hydroxy-1-Naphthaldehyde Schiff Base for Turn-on Fluorescence Detection of Zn2+ based on PET Mechanism

2021 ◽  
Author(s):  
Xinyue Mu ◽  
Liping Shi ◽  
Liqiang Yan ◽  
Ningli Tang
Keyword(s):  
Schiff Base ◽  
Detection Limit ◽  
Human Body ◽  
Rapid Detection ◽  
Zinc Ion ◽  
Living Cells ◽  
Quantitative Detection ◽  
Real Samples ◽  
Turn On ◽  
Low Cytotoxicity

Abstract Zinc ion is closely related to human health. Its content in human body is small, while the effect is large. However, it is not the more the better, must be in a scientific balance. Therefore, it is significant to the rapid detection of Zn2+ in the environment and organism. Herein, a fluorescent probe based on 2-hydroxy-1-naphthalene formaldehyde and furan-2-carbohydrazide was conveniently synthesized via Schiff base reaction. And this probe has been successfully applied to the accurate and quantitative detection of Zn2+ in real samples, showing turn on fluorescence, good selectivity, very low detection limit, real time response and reusability. In addition, this probe has the potential application to trace Zn2+ in living cells with low cytotoxicity.

scholarly journals Limit of detection in different matrices of nineteen commercially available rapid antigen tests for the detection of SARS-CoV-2

2021 ◽  
Author(s):  
Ana I. Cubas-Atienzar ◽  
Konstantina Kontogianni ◽  
Thomas Edwards ◽  
Dominic Wooding ◽  
Kate Buist ◽  
...  
Keyword(s):  
Culture Supernatant ◽  
Limit Of Detection ◽  
Culture Media ◽  
Performance Criteria ◽  
World Health ◽  
Analytical Sensitivity ◽  
Sample Type ◽  
Sensitivity Data ◽  
Antigen Tests ◽  
Health Organization

AbstractIn the context of the coronavirus disease 2019 (COVID-19) pandemic there has been an increase of the use of antigen-detection rapid diagnostic tests (Ag-RDT). The performance of Ag-RDT vary greatly between manufacturers and evaluating their analytical limit of detection (LOD) has become high priority. Here we describe a manufacturer-independent evaluation of the LOD of 19 marketed Ag-RDT using live SARS-CoV-2 spiked in different matrices: direct culture supernatant, a dry swab, and a swab in Amies. Additionally, the LOD using dry swab was investigated after 7 days’ storage at −80°C of the SARS-CoV-2 serial dilutions. An LOD of ≈ 5.0 × 102 pfu/ml (1.0 × 106 genome copies/ml) in culture media is defined as acceptable by the World Health Organization. Fourteen of nineteen Ag-RDTs (ActiveXpress, Espline, Excalibur, Innova, Joysbio, Mologic, NowCheck, Orient, PanBio, RespiStrip, Roche, Standard-F, Standard-Q and Sure-Status) exceeded this performance criteria using direct culture supernatant applied to the Ag-RDT. Six Ag-RDT were not compatible with Amies media and a decreased sensitivity of 2 to 20-fold was observed for eleven tests on the stored dilutions at −80°C for 7 days. Here, we provide analytical sensitivity data to guide appropriate test and sample type selection for use and for future Ag-RDT evaluations.

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