scholarly journals Optical Thickness-Encoded Suspension Array for High-Throughput Multiplexed Gene Detection

Sensors ◽  
2019 ◽  
Vol 19 (24) ◽  
pp. 5425
Author(s):  
Huiying Ma ◽  
Xuejing Chen ◽  
Bangrong Lu ◽  
Yanhong Ji
Keyword(s):  
Optical Thickness ◽  
Dna Detection ◽  
High Specificity ◽  
Analyte Concentration ◽  
Gene Detection ◽  
Suspension Array ◽  
Dna Molecule ◽  
Target Dna ◽  
Good Repeatability ◽  
Encoding Method

We proposed a coding and decoding method of suspension array (SA) based on micro-quartz pieces (MQPs) with different optical thicknesses. The capture probes (cDNA) were grafted onto the surfaces of MQPs and specifically recognized and combined with the partial sequence of the target DNA (tDNA) to form a MQP-cDNA-tDNA complex. Quantum dot-labeled signal probes were then used to specifically recognize and bind another portion of the tDNA in the complex to form a double-probe sandwich structure. This optical thickness-encoded SA can be decoded and detected by a dual-wavelength digital holographic phase fluorescence microscope system. We conducted a series of DNA molecule detection experiments by using this encoding method. Control experiments confirmed the specificity of optical thickness-encoded SA in DNA detection. The concentration gradient experiments then demonstrated the response of the MQPs based SA to analyte concentration. Finally, we used the encoding method to detect three types of DNA in a single sample and confirmed the feasibility of the proposed optical thickness-encoded SA in multiplexed DNA detection. The detection results are stable, and the detection exhibits high specificity and good repeatability.

Ion-chelation based digital barcodes for multiplexing of a suspension array

The Analyst ◽  
2019 ◽  
Vol 144 (13) ◽  
pp. 4093-4099 ◽  
Author(s):  
Guangxia Feng ◽  
Tian Guan ◽  
Qinghua He ◽  
Bangrong Lu ◽  
Xuejing Chen ◽  
...  
Keyword(s):  
Metal Ion ◽  
Suspension Array ◽  
Encoding Method ◽  
Coding Capacity

Our LIB-based metal ion encoding method can considerably expand coding capacity and ensure the accuracy of detection.

The Fabrication of 2D Cu-Based MOF Nanosheets for DNA Detection

2019 ◽  
Vol 72 (12) ◽  
pp. 939
Author(s):  
Xuan Qi ◽  
Lingyu Xia ◽  
Yunong Li ◽  
Tieqiang Wang ◽  
Xuemin Zhang ◽  
...  
Keyword(s):  
Detection Limit ◽  
Dna Detection ◽  
Metal Organic Framework ◽  
Linear Range ◽  
Spray Method ◽  
Target Dna ◽  
Metal Organic ◽  
Dna Mixture ◽  
2D Structure ◽  
Application Scope

The Cu-based metal–organic framework (MOF) analogues, copper 1,4-benzenedicarboxylate (CuBDC), copper 2,6-naphthalenedicarboxylate (Cu(2,6-NDC)), and copper 1,4-naphthalenedicarboxylate (Cu(1,4-NDC)) MOF nanosheets, are prepared as biosensor nanoplatforms for DNA detection by a spray method. With the ultrathin 2D structure, the fabricated MOF nanosheets exhibited better detection of target DNA, in particular when compared with the corresponding 3D MOF bulky crystals, when used as a DNA biosensor platform. The Cu(1,4-NDC) nanosheets display a distinct sensitivity with a detection limit of 0.3nM and linear range of 0–20nM, and selectivity for the target DNA or target DNA mixture. The feasible biosensor nanoplatform composed of 2D MOF nanosheets broadens the application scope of MOF nanosheets.

Simulation of Gold Nanoparticles Aggravating MEMS Cantilever Optical Static Detection Biochip

2013 ◽  
Vol 694-697 ◽  
pp. 966-970 ◽  
Author(s):  
Yue Tao Ge ◽  
Xiao Tong Yin
Keyword(s):  
Gold Nanoparticles ◽  
Low Cost ◽  
Mechanical Systems ◽  
Side Wall ◽  
High Sensitivity ◽  
Detection Sensitivity ◽  
Gene Detection ◽  
Micro Electro Mechanical Systems ◽  
Target Dna ◽  
Static Detection

A kind of gene detection biochip model based on biological micro electro mechanical systems (BioMEMS) technology and micro optical electro mechanical systems (MOEMS) technology is designed and simulated. In order to detect whether there are nucleic acid components in the testing samples, the biochip in this study issues horizontal light by laser, then receives and reads the deformation signals of MEMS cantilever by optical detector. The MEMS optical reflecting system can amplify MEMS cantilever deformation signal 22 times by micro reflectors which are set on the side wall of the cantilever free end. In order to improve optical detection sensitivity, gold nanoparticles (GNPs) which are combined with hybridization information is taken to aggravate MEMS cantilever, and employ Au - S chemical bond of GNPs and dithiol HS(CH2)6SH to combine and fix DNA probe, and then employ target DNA which is marked with biotin to combine GNPs by Biotin - Streptavidin combining. The simulation results show that this biochip can detect biological samples fast, high throughput, low cost, high sensitivity and reliably.

A dual-cycling biosensor for target DNA detection based on the toehold-mediated strand displacement reaction and exonuclease III assisted amplification

2018 ◽  
Vol 42 (6) ◽  
pp. 4714-4718 ◽  
Author(s):  
Yu Ling ◽  
Xiao Fang Zhang ◽  
Xiao Hui Chen ◽  
Li Liu ◽  
Xiao Hu Wang ◽  
...  
Keyword(s):  
Dna Detection ◽  
Dna Biosensor ◽  
Displacement Reaction ◽  
Strand Displacement ◽  
Exonuclease Iii ◽  
Target Dna ◽  
Strand Displacement Reaction

Based on the toehold-mediated strand displacement reaction and exonuclease III assisted amplification, a sensitive and simple target DNA biosensor was established.

scholarly journals Genetic evidence against intramolecular rejoining of the donor DNA molecule following IS10 transposition.

Genetics ◽  
1991 ◽  
Vol 128 (4) ◽  
pp. 687-694
Author(s):  
J Bender ◽  
J Kuo ◽  
N Kleckner
Keyword(s):  
Donor Site ◽  
Bacterial Genome ◽  
Strand Break ◽  
Donor Molecule ◽  
Dna Molecule ◽  
Target Dna ◽  
Donor Locus ◽  
Is10 Element ◽  
Original Donor ◽  
Original Information

Abstract Tn10 and IS10 transpose by a nonreplicative mechanism in which the transposon is excised from the donor molecule and integrated into a target DNA site, leaving behind a break at the original donor site. The fate of this broken donor DNA molecule is not known. We describe here two experiments that address this issue. One experiment demonstrates that a polar IS10 element gives rise to polarity-relief revertants at less than 1% the frequency of transposition of the same element in the same culture. In a second experiment, transpositions of an IS10 element from one site in the bacterial genome to another are selected and the resulting isolates examined for alterations at the donor site; none of 1088 such isolates exhibited a detectable change at the donor locus. These results are compatible with two possible fates of the transposon donor molecule: degradation ("donor suicide"), or restoration of the original information at the donor site by a recombinational repair mechanism analogous to double-strand break repair. These results argue against the possibility that the donor molecule gap is simply resealed by intramolecular rejoining.

scholarly journals The stepwise endonuclease activity of a thermophilic Argonaute protein

2019 ◽  
Author(s):  
Guanhua Xun ◽  
Qian Liu ◽  
Yuesheng Chong ◽  
Zhonglei Li ◽  
Xiang Guo ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Dna Cleavage ◽  
Pyrococcus Furiosus ◽  
Dna Amplification ◽  
Endonuclease Activity ◽  
Gene Detection ◽  
Complementary Sequences ◽  
Argonaute Proteins ◽  
Target Dna ◽  
Single Reaction

AbstractThermophilic Argonaute proteins (Agos) can function as endonucleases via specific guide-target base-pairing cleavage for host defense. The ability to cleave target DNA sequences at any arbitrary sites endows them with reprogramed DNA capacity. Here, we identify that an Ago from the hyperthermophilic archaeon Pyrococcus furiosus (PfAgo) shows a stepwise endonuclease activity, which is demonstrated by the double strand DNA cleavage directed by a single guide DNA rather than canonical one pair of guide DNAs. We reveal that the cleavage products with 5’-phosphorylated ends can used as the renewed guide which is capable to induce next round of cleavage to complementary sequences of target DNA. By combining the PfAgo stepwise endonuclease activity followed by target DNA amplification, we establish a rapid and specific platform for the unambiguously multiplex gene detection, termed RADAR (Renewed-gDNA Assisted DNA-cleavage by Argonaute). In the end, RADAR was applied to distinguish of human papillomavirus of serotypes in patient samples in a single reaction, suggesting that our technique would be adopted for diagnosing application.

Fabrication of fluorescence enhancement of quantum dots on a gold colloid formed film for oligonucleotide DNA detection

2017 ◽  
Vol 9 (3) ◽  
pp. 434-442 ◽  
Author(s):  
Jie Yang ◽  
Ji-Tao Song ◽  
Wei Hu ◽  
Yan Huang ◽  
Li-Juan Deng ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Quantum Dot ◽  
Fluorescence Enhancement ◽  
Dna Detection ◽  
Gold Colloid ◽  
Target Dna

This paper reported a method based on metal-enhanced quantum dot fluorescence on a gold colloid formed film for target DNA detection.

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