scholarly journals Multivariate Analysis of Difference Raman Spectra of the Irradiated Nucleus and Cytoplasm Region of SH-SY5Y Human Neuroblastoma Cells

Sensors ◽  
2019 ◽  
Vol 19 (18) ◽  
pp. 3971 ◽  
Author(s):  
Ines Delfino ◽  
Valerio Ricciardi ◽  
Lorenzo Manti ◽  
Maria Lasalvia ◽  
Maria Lepore
Keyword(s):  
Raman Spectra ◽  
Single Cells ◽  
Neuroblastoma Cells ◽  
Principal Component ◽  
X Rays ◽  
Human Neuroblastoma Cells ◽  
Difference Spectra ◽  
Human Neuroblastoma ◽  
X Ray

Previous works showed that spatially resolved Raman spectra of cytoplasm and nucleus region of single cells exposed to X-rays evidence different features. The present work aims to introduce a new approach to profit from these differences to deeper investigate X-ray irradiation effects on single SH-SY5Y human neuroblastoma cells. For this aim, Raman micro-spectroscopy was performed in vitro on single cells after irradiation by graded X-ray doses (2, 4, 6, 8 Gy). Spectra from nucleus and cytoplasm regions were selectively acquired. The examination by interval Principal Component Analysis (i-PCA) of the difference spectra obtained by subtracting each cytoplasm-related spectrum from the corresponding one detected at the nucleus enabled us to reveal the subtle modifications of Raman features specific of different spatial cell regions. They were discussed in terms of effects induced by X-ray irradiation on DNA/RNA, lipids, and proteins. The proposed approach enabled us to evidence some features not outlined in previous investigations.

scholarly journals An FTIR Microspectroscopy Ratiometric Approach for Monitoring X-ray Irradiation Effects on SH-SY5Y Human Neuroblastoma Cells

2020 ◽  
Vol 10 (8) ◽  
pp. 2974 ◽  
Author(s):  
Valerio Ricciardi ◽  
Marianna Portaccio ◽  
Lorenzo Manti ◽  
Maria Lepore
Keyword(s):  
Infrared Spectra ◽  
Neuroblastoma Cells ◽  
X Rays ◽  
Human Neuroblastoma ◽  
X Ray ◽  
Innovative Strategies ◽  
Recovery Processes ◽  
T24 Cells ◽  
Protein Nucleic Acid

The ability of Fourier transform infrared (FTIR) spectroscopy in analyzing cells at a molecular level was exploited for investigating the biochemical changes induced in protein, nucleic acid, lipid, and carbohydrate content of cells after irradiation by graded X-ray doses. Infrared spectra from in vitro SH-SY5Y neuroblastoma cells following exposure to X-rays (0, 2, 4, 6, 8, 10 Gy) were analyzed using a ratiometric approach by evaluating the ratios between the absorbance of significant peaks. The spectroscopic investigation was performed on cells fixed immediately (t0 cells) and 24 h (t24 cells) after irradiation to study both the initial radiation-induced damage and the effect of the ensuing cellular repair processes. The analysis of infrared spectra allowed us to detect changes in proteins, lipids, and nucleic acids attributable to X-ray exposure. The ratiometric analysis was able to quantify changes for the protein, lipid, and DNA components and to suggest the occurrence of apoptosis processes. The ratiometric study of Amide I band indicated also that the secondary structure of proteins was significantly modified. The comparison between the results from t0 and t24 cells indicated the occurrence of cellular recovery processes. The adopted approach can provide a very direct way to monitor changes for specific cellular components and can represent a valuable tool for developing innovative strategies to monitor cancer radiotherapy outcome.

scholarly journals In vitro targeting and specific transfection of human neuroblastoma cells by chCE7 antibody-mediated gene transfer

Gene Therapy ◽  
1997 ◽  
Vol 4 (2) ◽  
pp. 156-161 ◽  
Author(s):  
J-L Coll ◽  
E Wagner ◽  
V Combaret ◽  
K Metchler ◽  
H Amstutz ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Neuroblastoma Cells ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma

Raman micro-spectroscopy investigation on the effects of x-rays and polyphenols in human neuroblastoma cells

2019 ◽  
Author(s):  
Valerio Ricciardi ◽  
Giuseppe Perna ◽  
Maria Lasalvia ◽  
Ines Delfino ◽  
Lorenzo Manti ◽  
...  
Keyword(s):  
Neuroblastoma Cells ◽  
X Rays ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma

scholarly journals Human neuroblastoma cells acquire regulated secretory properties and different sensitivity to Ca2+ and alpha-latrotoxin after exposure to differentiating agents.

1989 ◽  
Vol 108 (6) ◽  
pp. 2291-2300 ◽  
Author(s):  
E Sher ◽  
S Denis-Donini ◽  
A Zanini ◽  
C Bisiani ◽  
F Clementi
Keyword(s):  
Electron Microscopy ◽  
De Novo ◽  
Neuroblastoma Cells ◽  
Calcium Ionophore ◽  
Membrane Receptors ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
Adequate Model ◽  
Human Neurons

IMR-32 human neuroblastoma cells are unable to release [3H]dopamine in response to secretagogues. However, they express a normal complement of membrane receptors and ion channels which are efficiently coupled to second messenger production. In the present study we took advantage of the ability of this cell line to differentiate in vitro in the presence of either dibutyrryl-cAMP or 5-bromodeoxyuridine, to analyze any developmentally regulated changes in its secretory properties. Uptake, storage, and release of [3H]dopamine were studied biochemically and by autoradiography. The calcium ionophore ionomycin, phorbol 12-myristate 13-acetate and the presynaptic acting neurotoxin alpha-latrotoxin were used in both control and differentiated cells as secretagogue agents. The presence of secretory organelles was investigated by electron microscopy; the expression of secretory organelle markers, such as chromogranin/secretogranin proteins (secretory proteins) and synaptophysin (membrane protein), was detected by Western blotting and immunofluorescence. The results obtained indicate that IMR-32 cells acquire regulated secretory properties after in vitro drug-induced differentiation: (a) they assemble "de novo" secretory organelles, as revealed by electron microscopy and detection of secretory organelle markers, and (b) they are able to store [3H]dopamine and to release the neurotransmitter in response to secretagogue stimuli. Furthermore, secretagogue sensitivity was found to be different, depending on the differentiating agent. In fact, dibutyrryl-cAMP treated cells release [3H]dopamine in response to alpha-latrotoxin, but not in response to ionomycin, whereas 5-bromodeoxyuridine treated cells release the neurotransmitter in response to both secretagogues. All together these results suggest that IMR-32 cells represent an adequate model for studying the development of the secretory apparatus in cultured human neurons.

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