scholarly journals Colorimetric Analysis of Glucose Oxidase-Magnetic Cellulose Nanocrystals (CNCs) for Glucose Detection

Sensors ◽  
2019 ◽  
Vol 19 (11) ◽  
pp. 2511 ◽  
Author(s):  
Ying Chuin Yee ◽  
Rokiah Hashim ◽  
Ahmad Ramli Mohd Yahya ◽  
Yazmin Bustami

Glucose oxidase (EC 1.1.3.4) sensors that have been developed and widely used for glucose monitoring have generally relied on electrochemical principle. In this study, the potential use of colorimetric method for glucose detection utilizing glucose oxidase-magnetic cellulose nanocrystals (CNCs) is explored. Magnetic cellulose nanocrystals (magnetic CNCs) were fabricated using iron oxide nanoparticles (IONPs) and cellulose nanocrystals (CNCs) via electrostatic self-assembly technique. Glucose oxidase was successfully immobilized on magnetic CNCs using carbodiimide-coupling reaction. About 33% of GOx was successfully attached on magnetic CNCs, and the affinity of GOx-magnetic CNCs to glucose molecules was slightly higher than free enzymes. Furthermore, immobilization does not affect the specificity of GOx-magnetic CNCs towards glucose and can detect glucose from 0.25 mM to 2.5 mM. Apart from that, GOx-magnetic CNCs stored at 4 °C for 4 weeks retained 70% of its initial activity and can be recycled for at least ten consecutive cycles.

2009 ◽  
Vol 54 (7) ◽  
pp. 1176-1181 ◽  
Author(s):  
XianXiang Wang ◽  
Shuo Huang ◽  
Zhi Shan ◽  
WanShen Yang

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Katalin Halász ◽  
Yanin Hosakun ◽  
Levente Csóka

Layer-by-layer electrostatic self-assembly technique was applied to improve the barrier properties of poly(lactic acid) (PLA) films and bottles. The LbL process was carried out by the alternate adsorption of chitosan (CH) (polycation) and cellulose nanocrystals (CNC) produced via ultrasonic treatment. Four bilayers (on each side) of chitosan and cellulose nanocrystals caused 29 and 26% improvement in barrier properties in case of films and bottles, respectively. According to the results the LbL process with CH and CNC offered a transparent “green” barrier coating on PLA substrates.


The Analyst ◽  
2019 ◽  
Vol 144 (5) ◽  
pp. 1831-1839 ◽  
Author(s):  
Chengke Wang ◽  
Jiangyu Li ◽  
Rong Tan ◽  
Qingqing Wang ◽  
Zexiang Zhang

Preparation of peroxidase-mimic ZnFe2O4–carbon nanotube (CNT)–glucose oxidase (GOD) composite material for sensitive detection of glucose.


2013 ◽  
Vol 737 ◽  
pp. 133-136 ◽  
Author(s):  
Miftakhul Huda ◽  
Jing Liu ◽  
Zulfakri bin Mohamad ◽  
You Yin ◽  
Sumio Hosaka

The self-assembly of block copolymer (BCP) has demonstrated as promising alternative technology to overcome the limitation of conventional lithography owing to its ability in forming nanostructure with size 3-100 nm. In this study, we investigated a technique to transfer self-assembled nanodots of Poly(styrene-b-dimethyl siloxane) (PS-PDMS) BCP to Si. The pattern transfer of PS-PDMS nanodots with the pitch of 33 nm and the diameter of 23 nm using CF4 etching with Carbon Hard Mask (CHM) as Mask is demonstrated. Si nanopillar with height of 51 nm was fabricated. This result improves the potential use of PS-PDMS BCP self-assembly technique for fabrication nano-electronic devices, such as quantum dot solar cell and ultrahigh density of magnetic recording.


Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1893
Author(s):  
Sónia O. Pereira ◽  
Nuno F. Santos ◽  
Alexandre F. Carvalho ◽  
António J. S. Fernandes ◽  
Florinda M. Costa

Carbon-based electrodes have demonstrated great promise as electrochemical transducers in the development of biosensors. More recently, laser-induced graphene (LIG), a graphene derivative, appears as a great candidate due to its superior electron transfer characteristics, high surface area and simplicity in its synthesis. The continuous interest in the development of cost-effective, more stable and reliable biosensors for glucose detection make them the most studied and explored within the academic and industry community. In this work, the electrochemistry of glucose oxidase (GOx) adsorbed on LIG electrodes is studied in detail. In addition to the well-known electroactivity of free flavin adenine dinucleotide (FAD), the cofactor of GOx, at the expected half-wave potential of −0.490 V vs. Ag/AgCl (1 M KCl), a new well-defined redox pair at 0.155 V is observed and shown to be related to LIG/GOx interaction. A systematic study was undertaken in order to understand the origin of this activity, including scan rate and pH dependence, along with glucose detection tests. Two protons and two electrons are involved in this reaction, which is shown to be sensitive to the concentration of glucose, restraining its origin to the electron transfer from FAD in the active site of GOx to the electrode via direct or mediated by quinone derivatives acting as mediators.


Author(s):  
Georgina Fabregat ◽  
Sonia Lanzalaco ◽  
Jules Aït Saïd ◽  
Xavier Muñoz-Pascual ◽  
Jordi Llorca ◽  
...  

2021 ◽  
pp. 138050
Author(s):  
Hani Farhat ◽  
Joel Célier ◽  
Claude Forano ◽  
Christine Mousty

RSC Advances ◽  
2014 ◽  
Vol 4 (46) ◽  
pp. 24369-24376 ◽  
Author(s):  
Jiemin Zhao ◽  
Xiaoping Wang ◽  
Yanshen Kuang ◽  
Yufeng Zhang ◽  
Xiaowen Shi ◽  
...  

Alginate (ALG)–lysozyme (LZ) beads were fabricated by a cross-linking process. Negatively charged ALG and positively charged LZ were alternately deposited on the positively charged ALG–LZ beads via a layer-by-layer (LBL) self-assembly technique.


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