scholarly journals Electrochemical Sensor Based on Poly(Azure B)-DNA Composite for Doxorubicin Determination

Sensors ◽  
2019 ◽  
Vol 19 (9) ◽  
pp. 2085 ◽  
Author(s):  
Anna Porfireva ◽  
Vyatseslav Vorobev ◽  
Sofya Babkina ◽  
Gennady Evtugyn
Keyword(s):  
Glassy Carbon ◽  
Limit Of Detection ◽  
Redox Properties ◽  
Carbon Electrode ◽  
Dna Sensor ◽  
Optimal Conditions ◽  
Cathodic Peak ◽  
Dna Concentration ◽  
Azure B ◽  
Application Mode

A new voltammetric DNA sensor has been developed for doxorubicin determination on the platform of a glassy carbon electrode (GCE) covered with electropolymerized Azure B film and physically adsorbed native DNA. The redox properties of polymeric Azure B were monitored at various pH and scan rates. DNA application decreased the peak currents related to polymeric and monomeric forms of the dye, whereas incubation in doxorubicin solution partially restored the peaks in accordance with the drug and DNA concentration. The relative shift of the cathodic peak current caused by doxorubicin depended on the nominal DNA concentration and its application mode. In optimal conditions, the DNA sensor makes it possible to determine between 0.1 μM to 0.1 nM doxorubicin (limit of detection 7 × 10−11 M). The DNA sensor was tested on commercial doxorubicin formulations and on artificial samples the mimicked electrolyte content of human serum.

Electrocatalytic redox behavior of graphene films towards acebutolol hydrochloride determination in real samples

2016 ◽  
Vol 40 (4) ◽  
pp. 3763-3772 ◽  
Author(s):  
Atmanand M. Bagoji ◽  
Sharanappa T. Nandibewoor
Keyword(s):  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Limit Of Detection ◽  
Graphene Film ◽  
Carbon Electrode ◽  
Redox Behavior ◽  
Optimal Conditions ◽  
Modified Glassy Carbon Electrode ◽  
Real Samples ◽  
Graphene Films

Under optimal conditions, a graphene film modified glassy carbon electrode exhibited a longer linearity range from 3.36 to 57.12 ng mL−1 of acebutolol with a limit of detection of 0.131 ng mL−1.

scholarly journals Method validation in quantitative electrochemical analysis of colchicine using glassy carbon electrode

2007 ◽  
Vol 5 (3) ◽  
pp. 766-778 ◽  
Author(s):  
Ede Bodoki ◽  
Robert Săndulescu ◽  
Liviu Roman
Keyword(s):  
Detection Limit ◽  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Limit Of Detection ◽  
Electrochemical Analysis ◽  
Carbon Electrode ◽  
Cathodic Peak ◽  
Limit Of Quantification ◽  
Validation Parameters

AbstractA cathodic differential pulse voltammetric determination of colchicine was validated using a glassy carbon electrode in HClO4/H3PO4 0.01 M. Colchicine gives an irreversible, diffusion-controlled peak at −862 mV vs. Ag/AgCl reference electrode. The cathodic peak is strongly influenced by a more alkaline environment with a shift towards more negative potentials. Method optimization was carried out in parallel for three types of electrodes (glassy carbon, mercury film and bismuth film coated glassy carbon). The cathodic peak current is higher using film-coated electrodes, but shows poorer intra-day reproducibility and a longer analysis time due to film renewal. Thus, a bare glassy carbon electrode was used to determine colchicine in the concentration range of 2.4 − 50 μg mL−1 (R 2 = 0.9998, n = 5), with a calculated detection limit of 0.80 μg mL−1. The proposed method was characterized according to ICH Harmonized Tripartite Guidance Q2(R1) by validation parameters (selectivity, linearity, accuracy, fidelity, limit of detection, limit of quantification) and it was successfully applied for the determination of colchicine from tablets, without the interference of the excipients. The method’s performances were evaluated and compared with both a known polarographic method and the official quantitative spectrophotometric determination from the Romanian Pharmacopoeia, Xth edition, respectively.

scholarly journals Simultaneous electrochemical sensing of dihydroxy benzene isomers at cost-effective allura red polymeric film modified glassy carbon electrode

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Pattan-Siddappa Ganesh ◽  
Ganesh Shimoga ◽  
Seok-Han Lee ◽  
Sang-Youn Kim ◽  
Eno E. Ebenso
Keyword(s):  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Limit Of Detection ◽  
Tap Water ◽  
Differential Pulse ◽  
Oxidation Potential ◽  
Electrochemical Sensing ◽  
Carbon Electrode ◽  
Buffer Solution ◽  
Allura Red

Abstract Background A simple and simultaneous electrochemical sensing platform was fabricated by electropolymerization of allura red on glassy carbon electrode (GCE) for the interference-free detection of dihydroxy benzene isomers. Methods The modified working electrode was characterized by electrochemical and field emission scanning electron microscopy methods. The modified electrode showed excellent electrocatalytic activity for the electrooxidation of catechol (CC) and hydroquinone (HQ) at physiological pH of 7.4 by cyclic voltammetric (CV) and differential pulse voltammetric (DPV) techniques. Results The effective split in the overlapped oxidation signal of CC and HQ was achieved in a binary mixture with peak to peak separation of 0.102 V and 0.103 V by CV and DPV techniques. The electrode kinetics was found to be adsorption-controlled. The oxidation potential directly depends on the pH of the buffer solution, and it witnessed the transfer of equal number of protons and electrons in the redox phenomenon. Conclusions The limit of detection (LOD) for CC and HQ was calculated to be 0.126 μM and 0.132 μM in the linear range of 0 to 80.0 μM and 0 to 110.0 μM, respectively, by ultra-sensitive DPV technique. The practical applicability of the proposed sensor was evaluated for tap water sample analysis, and good recovery rates were observed. Graphical abstract Electrocatalytic interaction of ALR/GCE with dihydroxy benzene isomers.

scholarly journals Electrochemical Detection of Endosulfan Using an AONP-PANI-SWCNT Modified Glassy Carbon Electrode

Materials ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 723
Author(s):  
Kgotla K. Masibi ◽  
Omolola E. Fayemi ◽  
Abolanle S. Adekunle ◽  
Amal M. Al-Mohaimeed ◽  
Asmaa M. Fahim ◽  
...  
Keyword(s):  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Limit Of Detection ◽  
Modified Electrodes ◽  
Antimony Oxide ◽  
Current Response ◽  
Carbon Electrode ◽  
Trace Detection ◽  
Limit Of Quantification ◽  
Walled Carbon Nanotubes

This report narrates the successful application of a fabricated novel sensor for the trace detection of endosulfan (EDS). The sensor was made by modifying a glassy-carbon electrode (GCE) with polyaniline (PANI), chemically synthesized antimony oxide nanoparticles (AONPs), acid-functionalized, single-walled carbon nanotubes (fSWCNTs), and finally, the AONP-PANI-SWCNT nanocomposite. The electrochemical properties of the modified electrodes regarding endosulfan detection were investigated via cyclic voltammetry (CV) and square-wave voltammetry. The current response of the electrodes to EDS followed the trend GCE-AONP-PANI-SWCNT (−510 µA) > GCE-PANI (−59 µA) > GCE-AONPs (−11.4 µA) > GCE (−5.52 µA) > GCE-fSWCNTs (−0.168 µA). The obtained results indicated that the current response obtained at the AONP-PANI-SWCNT/GCE was higher with relatively low overpotential compared to those from the other electrodes investigated. This demonstrated the superiority of the AONP-PANI-SWCNT-modified GCE. The AONP-PANI-SWCNT/GCE demonstrated good electrocatalytic activities for the electrochemical reduction of EDS. The results obtained in this study are comparable with those in other reports. The sensitivity, limit of detection (LoD), and limit of quantification (LoQ) of AONP-PANI-SWCNT/GCE towards EDS was estimated to be 0.0623 µA/µM, 6.8 µM, and 20.6 µM, respectively. Selectivity, as well as the practical application of the fabricated sensor, were explored, and the results indicated that the EDS-reduction current was reduced by only 2.0% when interfering species were present, whilst average recoveries of EDS in real samples were above 97%.

The influence of gold nanoparticles on the electroactivity of nickel tetrasulfonated phthalocyanine

2014 ◽  
Vol 18 (08n09) ◽  
pp. 642-651 ◽  
Author(s):  
Audacity Maringa ◽  
Tebello Nyokong
Keyword(s):  
Gold Nanoparticles ◽  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Glassy Carbon ◽  
Electrochemical Impedance ◽  
Modified Electrodes ◽  
Enhancement Effect ◽  
Carbon Electrode ◽  
Cathodic Peak ◽  
Peak Separation

We report on the electrodeposition of gold nanoparticles ( AuNPs ) on a glassy carbon electrode (GCE) followed by deposition of nickel tetrasulfonated phthalocyanine ( NiTSPc ) film by electropolymerization (poly- NiTSPc -GCE) to form Poly- NiTSPc / AuNPs -GCE. The presence of the gold nanoparticles caused a lowering of the anodic and cathodic peak separation (ΔE p ) of ferricyanide from 126 mV on poly- NiTSPc to 110 mV on poly- NiTSPc / AuNPs . The electrooxidation of nitrite improved on modified electrodes compared to GCE, with the latter giving E p = 0.78 V and the modified electrodes gave E p = 0.62 V or 0.61 V. Poly- NiTSPc / AuNPs -GCE had higher currents compared to poly- NiTSPc -GCE. This indicates the enhancement effect caused by the AuNPs . Electrochemical impedance spectroscopy and chronoamperometric studies also showed that poly- NiTSPc / AuNPs -GCE was a better electrocatalyst than poly- NiTSPc -GCE or AuNPs -GCE.

scholarly journals Stripping Voltammetric Determination of Analgesics in Their Pharmaceuticals Using Nano-Riboflavin-Modified Glassy Carbon Electrode

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Gopalakrishnan Gopu ◽  
Paramasivam Manisankar ◽  
Baladhandapani Muralidharan ◽  
Chinnapiyan Vedhi
Keyword(s):  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Limit Of Detection ◽  
Pharmaceutical Preparations ◽  
Sem Analysis ◽  
Carbon Electrode ◽  
Modified Glassy Carbon Electrode ◽  
Diffusion Controlled ◽  
Influence Of Ph

Cyclic voltammetric behaviors of three analgesics, acetaminophen (AAP), acetylsalicylic acid (ASA), and dipyrone (DP), were studied using nano-riboflavin-modified glassy carbon electrode. One well-defined oxidation peak each for AAP and ASA and three oxidation peaks for DP were observed. The influence of pH, scan rate, and concentration reveals irreversible diffusion controlled reaction. The SEM analysis confirmed good accumulation of the drugs on the electrode surface. Calibration was made under the maximum peak current conditions. The concentration range studied for the determination of drugs was 0.02 to 0.4 μg mL−1for AAP and ASA and 0.025 to 0.4 μg mL−1for DP. The lower limit of detection observed for AAP, ASA, and DP was 0.016, 0.007 μg mL−1, and 0.013 μg mL−1, respectively. The suitability of the method for the determination of these analgesics in pharmaceutical preparations and urine samples was also ascertained.

An Amperometric Biosensor Based on a Multi-Nanoporous SnO2/HRP/Chitosan for Detection of Hydrogen Peroxide in Human Urine Samples

2019 ◽  
Vol 17 (10) ◽  
pp. 769-776
Author(s):  
Samiul Alim ◽  
Rajan Jose ◽  
Mashitah M. Yusoff ◽  
A. K. M. Kafi
Keyword(s):  
Hydrogen Peroxide ◽  
Horseradish Peroxidase ◽  
Glassy Carbon ◽  
Human Urine ◽  
Limit Of Detection ◽  
Amperometric Biosensor ◽  
Carbon Electrode ◽  
Sample Analysis ◽  
Real Sample Analysis ◽  
Effects Of Ph

A new amperometric biosensor fabricated from a multi-nanoporous SnO2 and HRP was trialled. The multiporous SnO2 nanofiber was fabricated in the research lab. Horseradish peroxidase was co-immobilized on a glassy carbon electrode with the multiporous SnO2 nanofiber and chitosan. The FESEM and EDX analysis were used to study the nanostructure and composition details of the nanofiber. Electrochemical results established that the immobilized HRP exhibited direct electrochemical behavior toward hydrogen peroxide (H2O2). The effects of pH, and applied potentials on the biosensor were also investigated. The limit of detection which was found to be 5 × 10–7 M with the linear range being between 5 × 10–6 M to 1.2 × 10–4 M of H2O2. For real sample analysis, this biosensor showed that the H2O2 concentration in the human urine can be monitored conveniently, where the interference is noted to be negligible.

scholarly journals Voltammetric determination of dopamine in the presence of ascorbic and uric acids using partial least squares regression: determination of dopamine in human urine and plasma

2009 ◽  
Vol 7 (3) ◽  
pp. 524-531 ◽  
Author(s):  
Morteza Bahram ◽  
Khalil Farhadi ◽  
Farzin Arjmand
Keyword(s):  
Least Squares ◽  
Partial Least Squares ◽  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Limit Of Detection ◽  
Relative Standard Error ◽  
Carbon Electrode ◽  
Least Squares Regression ◽  
Relative Standard

AbstractA new differential pulse voltammetric method for dopamine determination at a bare glassy carbon electrode has been developed. Dopamine, ascorbic acid (AA) and uric acid (UA) usually coexist in physiological samples. Because AA and UA can be oxidized at potentials close to that of DA it is difficult to determine dopamine electrochemically, although resolution can be achieved using modified electrodes. Additionally, oxidized dopamine mediates AA oxidation and the electrode surface can be easily fouled by the AA oxidation product. In this work a chemometrics strategy, partial least squares (PLS) regression, has been applied to determine dopamine in the presence of AA and UA without electrode modification. The method is based on the electrooxidation of dopamine at a glassy carbon electrode in pH 7 phosphate buffer. The dopamine calibration curve was linear over the range of 1–313 μM and the limit of detection was 0.25 μM. The relative standard error (RSE %) was 5.28%. The method has been successfully applied to the measurement of dopamine in human plasma and urine.

scholarly journals Electrochemical determination of acetaminophen at a carbon electrode modified in the presence of β-cyclodextrin: role of the activated glassy carbon and the electropolymerised β-cyclodextrin

2021 ◽  
Author(s):  
Bronach Healy ◽  
Francesco Rizzuto ◽  
Marida de Rose ◽  
Tian Yu ◽  
Carmel B. Breslin
Keyword(s):  
Glassy Carbon ◽  
Electrochemical Sensors ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
Electrochemical Determination ◽  
Phosphate Solution ◽  
Carbon Electrode ◽  
Provide Pain Relief ◽  
High Concentrations

AbstractAcetaminophen is a well-known drug commonly used to provide pain relief, but it can also lead to acute liver failure at high concentrations. Therefore, there is considerable interest in monitoring its concentrations. Sensitive and selective acetaminophen electrochemical sensors were designed by cycling a glassy carbon electrode (GCE) to high potentials in the presence of β-CD in a phosphate electrolyte, or by simply activating the GCE electrode in the phosphate solution. Using cyclic voltammetry, adsorption-like voltammograms were recorded. The acetaminophen oxidation product, N-acetyl benzoquinone imine, was protected from hydrolysis, and this was attributed to the adsorption of acetaminophen at the modified GCE. The rate constants for the oxidation of acetaminophen were estimated as 4.3 × 10–3 cm2 s–1 and 3.4 × 10–3 cm2 s–1 for the β-CD-modified and -activated electrodes, respectively. Using differential pulse voltammetry, the limit of detection was calculated as 9.7 × 10–8 M with a linear concentration range extending from 0.1 to 80 μM. Furthermore, good selectivity was achieved in the presence of caffeine, ascorbic acid and aspirin, enabling the determination of acetaminophen in a commercial tablet. Similar electrochemical data were obtained for both the β-CD-modified and activated GCE surfaces, suggesting that the enhanced detection of acetaminophen is connected mainly to the activation and oxidation of the GCE. Using SEM, EDX and FTIR, no evidence was obtained to indicate that the β-CD was electropolymerised at the GCE.

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