scholarly journals Hand-held Colorimetry Sensor Platform for Determining Salivary α-Amylase Activity and Its Applications for Stress Assessment

Sensors ◽  
2019 ◽  
Vol 19 (7) ◽  
pp. 1571 ◽  
Author(s):  
Hsien-Yi Hsiao ◽  
Richie Chen ◽  
Chih-Chi Chou ◽  
Tzong-Jih Cheng

This study develops a hand-held stress assessment meter with a chemically colorimetric strip for determining salivary α-amylase activity, using a 3,5 dinitrosalicylic acid (DNS) assay to quantify the reducing sugar released from soluble starch via α-amylase hydrolysis. The colorimetric reaction is produced by heating the strip with a mini polyester heater plate at boiling temperature to form a brick red colored product, which measured at 525 nm wavelength. This investigation describes in detail the design, construction, and performance evaluation of a hand-held α-amylase activity colorimeter with a light emitted diode (LED) and photo-detector with built-in filters. The dimensions and mass of the proposed prototype are only 120 × 60 × 60 mm3 and 200 g, respectively. This prototype has an excellent correlation coefficient (>0.995), comparable with a commercial ultraviolet–visible spectroscope, and has a measurable α-amylase activity range of 0.1–1.0 U mL−1. The hand-held device can measure the salivary α-amylase activity with only 5 μL of saliva within 12 min of testing. This sensor platform effectively demonstrates that the level of salivary α-amylase activity increases more significantly than serum cortisol, the other physiological stressor biomarker, under physiologically stressful exercise conditions. Thus, this work demonstrates that the hand-held α-amylase activity meter is an easy to use and cost-effective stress assessment tool for psychoneuroendocrinology research.

2021 ◽  
pp. bjsports-2020-103696
Author(s):  
Richard Weiler ◽  
Cheri Blauwet ◽  
David Clarke ◽  
Kristine Dalton ◽  
Wayne Derman ◽  
...  

Concussion is a frequent injury in many sports and is also common in para athletes. However, there is a paucity of concussion research related to para sport, and prior International Concussion in Sport (CIS) consensus papers have not substantively addressed this population. To remedy this and to improve concussion care provided to para athletes, the concussion in para sport (CIPS) multidisciplinary expert group was created. This group analysed and discussed in-depth para athlete-specific issues within the established key clinical domains of the current (2017) consensus statement on CIS. Due to the onset of the COVID-19 pandemic, the group held all meetings by video conferencing. The existing Sport Concussion Assessment Tool 5 (SCAT5) for the immediate on-field and office-based off-field assessment of concussion was evaluated as part of this process, to identify any para athlete-specific concerns. Regular preparticipation and periodic health examinations are essential to determine a baseline reference point for concussion symptoms but pose additional challenges for the interpreting clinician. Further considerations for concussion management for the para athlete are required within the remove, rest, reconsider and refer consensus statement framework. Considering return to sport (RTS), the 2017 CIS consensus statement has limitations when considering the RTS of the para athlete. Case-by-case decision making related to RTS following concussion is imperative for para athletes. Additional challenges exist for the evaluation and management of concussion in para athletes. There is a need for greater understanding of existing knowledge gaps and attitudes towards concussion among athlete medical staff, coaches and para athletes. Future research should investigate the use and performance of common assessment tools in the para athlete population to better guide their clinical application and inform potential modifications. Concussion prevention strategies and sport-specific rule changes, such as in Para Alpine Skiing and Cerebral Palsy Football, also should be carefully considered to reduce the occurrence of concussion in para athletes.


Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1437
Author(s):  
Jing Yi Ong ◽  
Andrew Pike ◽  
Ling Ling Tan

The presence of mycotoxins in foodstuffs and feedstuffs is a serious concern for human health. The detection of mycotoxins is therefore necessary as a preventive action to avoid the harmful contamination of foodstuffs and animal feed. In comparison with the considerable expense of treating contaminated foodstuffs, early detection is a cost-effective way to ensure food safety. The high affinity of bio-recognition molecules to mycotoxins has led to the development of affinity columns for sample pre-treatment and the development of biosensors for the quantitative analysis of mycotoxins. Aptamers are a very attractive class of biological receptors that are currently in great demand for the development of new biosensors. In this review, the improvement in the materials and methodology, and the working principles and performance of both conventional and recently developed methods are discussed. The key features and applications of the fundamental recognition elements, such as antibodies and aptamers are addressed. Recent advances in aptasensors that are based on different electrochemical (EC) transducers are reviewed in detail, especially from the perspective of the diagnostic mechanism; in addition, a brief introduction of some commercially available mycotoxin detection kits is provided.


Sensors ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1342
Author(s):  
Borja Nogales ◽  
Miguel Silva ◽  
Ivan Vidal ◽  
Miguel Luís ◽  
Francisco Valera ◽  
...  

5G communications have become an enabler for the creation of new and more complex networking scenarios, bringing together different vertical ecosystems. Such behavior has been fostered by the network function virtualization (NFV) concept, where the orchestration and virtualization capabilities allow the possibility of dynamically supplying network resources according to its needs. Nevertheless, the integration and performance of heterogeneous network environments, each one supported by a different provider, and with specific characteristics and requirements, in a single NFV framework is not straightforward. In this work we propose an NFV-based framework capable of supporting the flexible, cost-effective deployment of vertical services, through the integration of two distinguished mobile environments and their networks: small sized unmanned aerial vehicles (SUAVs), supporting a flying ad hoc network (FANET) and vehicles, promoting a vehicular ad hoc network (VANET). In this context, a use case involving the public safety vertical will be used as an illustrative example to showcase the potential of this framework. This work also includes the technical implementation details of the framework proposed, allowing to analyse and discuss the delays on the network services deployment process. The results show that the deployment times can be significantly reduced through a distributed VNF configuration function based on the publish–subscribe model.


2018 ◽  
Vol 6 (5) ◽  
pp. 972-979 ◽  
Author(s):  
Tae-Yeon Hwang ◽  
Yomin Choi ◽  
YoSeb Song ◽  
Nu Si A. Eom ◽  
Seil Kim ◽  
...  

Monodispersed nano Pt catalyzed linear dense assembly of SWCNTs bundles on a noble multi-layered electrode system for cost-effective hydrogen sensor.


Author(s):  
Kala Meah ◽  
Steven Fletcher ◽  
Yu Wan ◽  
Sadrul Ula

Many parts of the western US is rural in nature and consequently do not have electrical distribution lines in many parts of farms and ranches. Distribution line extension costs can run from $15,000 to $25,000 per mile, thereby making availability of electricity to small water pumping projects economically unattractive. Solar photo-voltaic (PV) powered water pumping is more cost effective in these small scale applications. Many western states including Wyoming are passing through fifth year of drought with the consequent shortages of water for many applications. Wyoming State Climatologist is predicting a possible 5–10 years of drought. Drought impacts the surface water right away, while it takes much longer to impact the underground aquifers. To mitigate the effect on the livestock and wildlife, Wyoming Governor Dave Freudenthal initiated a solar water pumping initiative in cooperation with the University of Wyoming, County Conservation Districts, Rural Electric Cooperatives, and ranching organizations. Solar water pumping has several advantages over traditional systems; for example, diesel or propane engines require not only expensive fuels, they also create noise and air pollution in many remote pristine areas. Solar systems are environment friendly, low maintenance and have no fuel cost. In this paper the design, installation and performance monitoring of the solar system for small scale remote water pumping will be presented.


Author(s):  
Will Judge ◽  
Georges Kipouros

The production of aluminum alloys through powder metallurgy (PM) processes allows for the manufacture of net- or near-net-shape components in a cost-effective and sustainable manner. The high reactivity of aluminum metal, however, complicates PM processing, and special attention must be given to certain steps during production, particularly sintering. PM processing conditions strongly affect the structure and porosity of aluminum PM alloys, which ultimately determine their material properties and performance. In this article, the fundamental aspects of the commercial production of aluminum PM alloys are presented, along with the effects of production conditions on the structure and porosity of aluminum PM alloys. The properties and performance of aluminum PM alloys are then analyzed and interpreted with respect to their structure and porosity.


2018 ◽  
Vol 8 (4) ◽  
pp. 688-697 ◽  
Author(s):  
Anu Rachel Thomas ◽  
Martin Kranert ◽  
Ligy Philip

Abstract Septage management is a challenging task across India as more than 44% of the population depends on septic tanks for human waste management. Septage collected from Chennai city, India, was found to be rich in nutrients even though the total solids content (<2%) and C/N are low. The current practice of septage disposal in Chennai city is co-treatment in existing sewage treatment plants. The main drawback of this method is that treatment efficiency could deteriorate if the organic load increased much beyond the design load of the treatment plant and would result in poor treated-water quality. In this context, the present study focused on the potential of in-vessel co-composting as a sustainable treatment option. In order to enhance the co-composting process, suitable co-substrates were identified for making the process efficient and cost-effective. The area under the temperature profile during composting was used as an assessment tool for the identification of the proper mix. Addition of mixed organic waste and coir pith waste (bulking agent) to the dewatered septage gave the highest area among the different combinations. Different waste combinations were investigated in order to assess the suitability for field scale application.


Author(s):  
Nancy Rodrigues ◽  
Maureen Kelly ◽  
Tobi Henderson

IntroductionThree Canadian clinical-administrative hospital databases were linked to the Canadian Vital Statistics Death Database (CVSD) to provide information about patients who died following discharge from hospital as well as supplementary information about patients that died in-hospital. Quality was assessed using a guided approach and through feedback from initial users. Objectives and ApproachThe linked datasets were created to develop and validate health care indicators and performance measures and perform outcome analyses. It is therefore imperative to evaluate the data’s fitness for use. Quality was assessed by calculating coverage of deaths for all linked contributors, creating a profile of the linked dataset and analyzing issues that were identified by users. These analyses were guided by an existing Data Source Assessment Tool, which provides a set of criteria that allow for assessment across five dimensions of quality, thus allowing for appropriate determination of a given set of data’s fitness for use. ResultsDeterministic linkage of the datasets resulted in linkage rates that ranged from 66.9% to 90.9% depending on the dataset or data year. Linkage rates also varied by Canadian jurisdictions and patient cohort. Variables had good data availability with rates of 95% or higher. Initial users identified a significant number of duplicate records that were flagged to and corrected by the data supplier. 1.4\% of acute hospital deaths had discrepancies in the death date captured in the two linked sources; the vast majority had a difference of only one day. A user group and issue tracking process were created to share information about the linked data and guarantee that issues are triaged to the appropriate party and allow for timely follow up with the data supplier. Conclusion/ImplicationsDocumentation provided by the data supplier was vital to understanding the linkage methodology and its impact on linkage rates. A guided data assessment ensured that strengths and limitations were identified and shared to support appropriate use. Feedback to the data supplier is supporting ongoing improvements to the linkage methodology.


2004 ◽  
Vol 17 (1) ◽  
pp. 61-70 ◽  
Author(s):  
Daniel Denison ◽  
Colleen Lief ◽  
John L. Ward

Through years of consulting experience and culture research, a fuller picture of family firms began to emerge. It became increasingly clear that family business sustainability and accomplishment were rooted in something deeper, something beyond superficial explanation. Belief in the innate value and uniqueness of family business culture drove collaboration on this project between the disciplines of family business and organizational behavior. The goal was to critically examine family business culture and performance relative to nonfamily firms. The Denison Organizational Culture Survey, a cultural assessment tool that has linked corporate culture to financial performance, was administered to a sample of 20 family businesses and 389 nonfamily businesses, allowing us to compare their cultures. The results showed that the corporate cultures of family enterprises were more positive than the culture of firms without a family affiliation. Family enterprises scored higher on all 12 dimensions of the assessment tool. Despite the small sample, several of these differences were statistically significant. This suggests that family firms perform better because of who they are. In addition, recent research that shows they also perform better because of what they do strategically. Their histories and shared identities provide a connectedness to time-tested core values and standards of behavior that lead to bottom-line success.


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