scholarly journals Electrochemical DNA Sensor Based on Carbon Black—Poly(Neutral Red) Composite for Detection of Oxidative DNA Damage

Sensors ◽  
2018 ◽  
Vol 18 (10) ◽  
pp. 3489 ◽  
Author(s):  
Yurii Kuzin ◽  
Dominika Kappo ◽  
Anna Porfireva ◽  
Dmitry Shurpik ◽  
Ivan Stoikov ◽  
...  
Keyword(s):  
Carbon Black ◽  
Oxidative Dna Damage ◽  
Electrochemical Impedance ◽  
Neutral Red ◽  
Redox Activity ◽  
Antitumor Drugs ◽  
Oxygen Species ◽  
Dna Sensor ◽  
Electrochemical Dna Sensor ◽  
Polymeric Dye

Voltammetric DNA sensor has been proposed on the platform of glassy carbon electrode covered with carbon black with adsorbed pillar[5]arene molecules. Electropolymerization of Neutral Red performed in the presence of native or oxidatively damaged DNA resulted in formation of hybrid material which activity depended on the DNA conditions. The assembling of the surface layer was confirmed by scanning electron microscopy and electrochemical impedance spectroscopy. The influence of DNA and pillar[5]arene on redox activity of polymeric dye was investigated and a significant increase of the peak currents was found for DNA damaged by reactive oxygen species generated by Cu2+/H2O2 mixture. Pillar[5]arene improves the electron exchange conditions and increases the response and its reproducibility. The applicability of the DNA sensor developed was shown on the example of ascorbic acid as antioxidant. It decreases the current in the concentration range from 1.0 μM to 1.0 mM. The possibility to detect antioxidant activity was qualitatively confirmed by testing tera infusion. The DNA sensor developed can find application in testing of carcinogenic species and searching for new antitumor drugs.

scholarly journals Electrochemical DNA Sensor Based on Poly(Azure A) Obtained from the Buffer Saturated with Chloroform

Sensors ◽  
2021 ◽  
Vol 21 (9) ◽  
pp. 2949
Author(s):  
Anna Porfireva ◽  
Kseniya Plastinina ◽  
Vladimir Evtugyn ◽  
Yurii Kuzin ◽  
Gennady Evtugyn
Keyword(s):  
Dna Damage ◽  
Cyclic Voltammetry ◽  
Quartz Crystal ◽  
Electrochemical Impedance ◽  
Polymer Surface ◽  
Charge Transfer Resistance ◽  
Dna Sensor ◽  
Transfer Resistance ◽  
Electrochemical Dna Sensor ◽  
Azure A

Electropolymerized redox polymers offer broad opportunities in detection of biospecific interactions of DNA. In this work, Azure A was electrochemically polymerized by multiple cycling of the potential in phosphate buffer saturated with chloroform and applied for discrimination of the DNA damage. The influence of organic solvent on electrochemical properties of the coating was quantified and conditions for implementation of DNA in the growing polymer film were assessed using cyclic voltammetry, quartz crystal microbalance, and electrochemical impedance spectroscopy. As shown, both chloroform and DNA affected the morphology of the polymer surface and electropolymerization efficiency. The electrochemical DNA sensor developed made it possible to distinguish native and thermally and chemically damaged DNA by changes in the charge transfer resistance and capacitance.

Multi-walled Carbon Nanotubes/Manganese Dioxide Nano- flowers-like/Polyaniline Nanowires Nanocomposite Modified Electrode: A New Platform for a Highly Sensitive Electrochemical Impedance DNA Sensor

2021 ◽  
Author(s):  
Tuan Chu ◽  
Luyen Thi Tran ◽  
Hoang Vinh Tran ◽  
Trung Tran ◽  
Nghia Trong Nguyen ◽  
...  
Keyword(s):  
Electrochemical Impedance ◽  
Dna Sensor ◽  
Label Free ◽  
Dna Sensors ◽  
Electrochemical Dna Sensor ◽  
Target Dna ◽  
Highly Sensitive ◽  
Polyaniline Nanowires ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

Abstract We describe in this report a development of label-free electrochemical DNA sensor based on a novel nanostructured electrode of multi-walled carbon nanotubes (MWCNTs)/ nano-flowers-like manganese dioxide (MnO2)/polyaniline nanowires (PANi NWs) nanocomposite. The nanocomposite was synthesized in-situ onto an interdigitated platinum microelectrode (Pt) using a combination of chemical and electrochemical synthesis methods: chemical preparation of MWCNTs/MnO2 and electropolymerization of PANi NWs. The fabricated MWCNTs/MnO2/PANi NWs was then used to develop a label-free electrochemical DNA sensor for a specific gene of Escherichia coli (E.coli) O157:H7 detection. The MWCNTs/MnO2/PANi NWs modified Pt electrode’s surface can facilitate for probe DNA strands immobilization and, therefore the electrochemical signal of the DNA sensors has been improved. The electrochemical impedance spectroscopy (EIS) measurements were conducted to investigate the output signals generated by the specific binding of probe and target DNA sequences. Obtained results indicated that the developed electrochemical biosensor can detect the target DNA in the linear range of 5 pM to 500 nM with a low limit of detection (LOD) at 4.42 × 10 –13 M. The research results demonstrated that the MWCNTs/MnO2/PANi NWs nanocomposite-based electrochemical DNA sensor has a great potential application to the development of highly sensitive and selective electrochemical DNA sensors to detect pathogenic agents.

Silver Nanoparticles Decorated Polyaniline Nanowires-Based Electrochemical DNA Sensor: Two-step Electrochemical Synthesis

2020 ◽  
Vol 167 (8) ◽  
pp. 087508 ◽  
Author(s):  
Luyen Thi Tran ◽  
Hoang Vinh Tran ◽  
Hue Thi Minh Dang ◽  
Chinh Dang Huynh ◽  
Tuan Anh Mai
Keyword(s):  
Silver Nanoparticles ◽  
Electrochemical Synthesis ◽  
Dna Sensor ◽  
Electrochemical Dna Sensor ◽  
Polyaniline Nanowires

scholarly journals Sensitive electrochemical DNA sensor for the detection of HIV based on a polyaniline/graphene nanocomposite

2019 ◽  
Vol 5 (2) ◽  
pp. 313-319 ◽  
Author(s):  
Qiaojuan Gong ◽  
Haixia Han ◽  
Haiying Yang ◽  
Meiling Zhang ◽  
Xiaoling Sun ◽  
...  
Keyword(s):  
Dna Sensor ◽  
Electrochemical Dna Sensor ◽  
Graphene Nanocomposite

ELECTROCHEMICAL DNA SENSOR TECHNOLOGY FOR MONITORING OF DRUG–DNA INTERACTIONS

NANO ◽  
2008 ◽  
Vol 03 (04) ◽  
pp. 229-232 ◽  
Author(s):  
A. ERDEM ◽  
H. KARADENIZ ◽  
A. CALISKAN ◽  
A. VASEASHTA
Keyword(s):  
Drug Delivery ◽  
Small Molecules ◽  
Anticancer Drugs ◽  
Point Of Care ◽  
Sensor Technology ◽  
Electrochemical Biosensors ◽  
Dna Sensor ◽  
Dna Interactions ◽  
Electrochemical Dna Sensor

The objective of this investigation is to understand the nature and dynamics of binding small molecules to bio-macromolecules using electrochemical methods. The investigation pertaining to the design of site- and conformation-specific reagents provides a rationale for new studies of drug delivery design. Some anticancer drugs and DNA interactions have been undertaken by using a variety of techniques. Determination of interaction between DNA and DNA-targeted molecules would be valuable in the design of molecule-specific electrochemical biosensors for applications in diagnostics, development of drugs for chemotherapy, and as a biotechnological tool for DNA-based point-of-care diagnosis.

Sign in / Sign up

Export Citation Format

Share Document