scholarly journals SPR Biosensors in Direct Molecular Fishing: Implications for Protein Interactomics

Sensors ◽  
2018 ◽  
Vol 18 (5) ◽  
pp. 1616 ◽  
Author(s):  
Anna Florinskaya ◽  
Pavel Ershov ◽  
Yuri Mezentsev ◽  
Leonid Kaluzhskiy ◽  
Evgeniy Yablokov ◽  
...  
Keyword(s):  
Molecular Fishing

Direct Molecular Fishing of New Protein Partners for Human Thromboxane Synthase

Acta Naturae ◽  
2017 ◽  
Vol 9 (4) ◽  
pp. 92-100 ◽  
Author(s):  
A. V. Svirid ◽  
◽  
P. V. Ershov ◽  
E. O. Yablokov ◽  
L. A. Kaluzhskiy ◽  
...  
Keyword(s):  
Thromboxane Synthase ◽  
Protein Partners ◽  
Molecular Fishing ◽  
New Protein

Protein interactomics based on direct molecular fishing on paramagnetic particles: Experimental simulation and SPR validation

PROTEOMICS ◽  
2012 ◽  
Vol 12 (22) ◽  
pp. 3295-3298 ◽  
Author(s):  
Pavel Ershov ◽  
Yury Mezentsev ◽  
Oksana Gnedenko ◽  
Dmitry Mukha ◽  
Aleksei Yantsevich ◽  
...  
Keyword(s):  
Experimental Simulation ◽  
Molecular Fishing

Synthesizing molecular fishing nets

Physics Today ◽  
2018 ◽  
Vol 71 (5) ◽  
pp. 40-47 ◽  
Author(s):  
Marco Servalli ◽  
Hans Christian Öttinger ◽  
A. Dieter Schlüter
Keyword(s):  
Molecular Fishing

Direct Molecular Fishing of Zinc-Dependent Protein Partners of Amyloid-beta 1–16 with the Taiwan (D7H) Mutation and Phosphorylated Ser8 Residue

2020 ◽  
Vol 54 (6) ◽  
pp. 904-910
Author(s):  
P. V. Ershov ◽  
Yu. V. Mezentsev ◽  
E. O. Yablokov ◽  
L. A. Kaluzgskiy ◽  
A. S. Ivanov ◽  
...  
Keyword(s):  
Amyloid Beta ◽  
Protein Partners ◽  
Molecular Fishing ◽  
Dependent Protein

A coordinated molecular ‘fishing’ mechanism in heterodimeric kinesin

2010 ◽  
Vol 7 (3) ◽  
pp. 036003 ◽  
Author(s):  
Ruizheng Hou ◽  
Zhisong Wang
Keyword(s):  
Molecular Fishing

Direct molecular fishing in molecular partners investigation in protein–protein and protein–peptide interactions

2016 ◽  
Vol 42 (1) ◽  
pp. 14-21 ◽  
Author(s):  
A. S. Ivanov ◽  
P. V. Ershov ◽  
A. A. Molnar ◽  
Yu. V. Mezentsev ◽  
L. A. Kaluzhskiy ◽  
...  
Keyword(s):  
Peptide Interactions ◽  
Molecular Fishing

scholarly journals Defining Elastic Fiber Interactions by Molecular Fishing

2009 ◽  
Vol 8 (12) ◽  
pp. 2715-2732 ◽  
Author(s):  
Stuart A. Cain ◽  
Amanda McGovern ◽  
Elaine Small ◽  
Lyle J. Ward ◽  
Clair Baldock ◽  
...  
Keyword(s):  
Elastic Fiber ◽  
Molecular Fishing

Direct Molecular Fishing of New Protein Partners for Human Thromboxane Synthase

Acta Naturae ◽  
2017 ◽  
Vol 9 (4) ◽  
pp. 92-100 ◽  
Author(s):  
A. V. Svirid ◽  
◽  
P. V. Ershov ◽  
E. O. Yablokov ◽  
L. A. Kaluzhskiy ◽  
...  
Keyword(s):  
Thromboxane Synthase ◽  
Protein Partners ◽  
Molecular Fishing ◽  
New Protein

Optical surface plasmon resonance biosensors in molecular fishing

2015 ◽  
Vol 61 (2) ◽  
pp. 231-238 ◽  
Author(s):  
A.S. Ivanov ◽  
A.E. Medvedev
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Protein Identification ◽  
Optical Biosensor ◽  
Coupled Processes ◽  
Affinity Separation ◽  
Spr Biosensor ◽  
Biological Mixtures ◽  
Molecular Fishing

An optical biosensor employing surface plasmon resonance is a highly efficient instrument applicable for direct real time registration of molecular interactions without additional use of any labels or coupled processes. As an independent approach it is especially effective in analysis of various ligand receptor interactions. SPR-biosensors are used for validation of studies on intermolecular interactions in complex biological systems (affinity profiling of various groups of proteins, etc.). Recently, potential application of the SPR-biosensor for molecular fishing (direct affinity binding of target molecules from complex biological mixtures on the optical biosensor surface followed by their elution for identification by LC-MS/MS) has been demonstrated. Using SPR-biosensors in such studies it is possible to solve the following tasks: (a) SPR-based selection of immobilization conditions required for the most effective affinity separation of a particular biological sample; (b) SPR-based molecular fishing for subsequent protein identification by mass spectrometry; (c) SPR-based validation of the interaction of identified proteins with immobilized ligand. This review considers practical application of the SPR technology in the context of recent studies performed in the Institute of Biomedical Chemistry on molecular fishing of real biological objects.

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