scholarly journals A Label-Free Fluorescent Array Sensor Utilizing Liposome Encapsulating Calcein for Discriminating Target Proteins by Principal Component Analysis

Sensors ◽  
2017 ◽  
Vol 17 (7) ◽  
pp. 1630 ◽  
Author(s):  
Ryota Imamura ◽  
Naoki Murata ◽  
Toshinori Shimanouchi ◽  
Kaoru Yamashita ◽  
Masayuki Fukuzawa ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Principal Component ◽  
Component Analysis ◽  
Label Free ◽  
Target Proteins ◽  
Array Sensor

Label-free detection of biotoxins via a photo-induced force infrared spectrum at the single-molecular level

The Analyst ◽  
2019 ◽  
Vol 144 (20) ◽  
pp. 6108-6117 ◽  
Author(s):  
Bin Ji ◽  
Ahmad Kenaan ◽  
Shan Gao ◽  
Jin Cheng ◽  
Daxiang Cui ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Infrared Spectrum ◽  
Single Molecule ◽  
Molecular Level ◽  
Principal Component ◽  
Component Analysis ◽  
Label Free ◽  
Force Microscopy ◽  
Label Free Detection

Schematic illustration of photo-induced force microscopy combine principal component analysis detected and distinguish single molecule particles of biotoxins AT, RT/ETX with label-free.

Characteristic of Five Subpopulation Leukocytes in Single-Cell Levels Based on Partial Principal Component Analysis Coupled with Raman Spectroscopy

2020 ◽  
Vol 74 (12) ◽  
pp. 1463-1472
Author(s):  
Wenxue Li ◽  
Liu Wang ◽  
Chuan Luo ◽  
Zhiqiang Zhu ◽  
Jianlong Ji ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Raman Spectroscopy ◽  
Wavelet Transform ◽  
Single Cell ◽  
Low Frequency ◽  
Principal Component ◽  
Component Analysis ◽  
Label Free ◽  
Source Of Infection ◽  
Biochemical Features

Characteristics of five subpopulation leukocytes in single-cell levels based on partial principal component analysis coupled with Raman spectroscopy were proposed to recognize the biochemical features of five subpopulation leukocytes. Using wavelet transform, the reconstructed spectra of the low-frequency wavelet coefficients were used to perform multiple principal component analysis based on segmented spectral data wreathing cover at 720–800 cm–1, 840–994 cm–1, and 1010–1070 cm–1 wavenumbers, respectively. Our approach is promising since it enables to establish a better understanding of the underlying molecular difference between the subtypes of leukocytes in a label-free manner and to estimate the source of infection.

A German version of the Intermittent Claudication Questionnaire (ICQ): cultural adaptation and validation

VASA ◽  
2012 ◽  
Vol 41 (5) ◽  
pp. 333-342 ◽  
Author(s):  
Kirchberger ◽  
Finger ◽  
Müller-Bühl
Keyword(s):  
Principal Component Analysis ◽  
Intermittent Claudication ◽  
Completion Time ◽  
Short Form ◽  
Principal Component ◽  
Component Analysis ◽  
German Version ◽  
Average Completion Time ◽  
Sf 36 ◽  
Related Quality

Background: The Intermittent Claudication Questionnaire (ICQ) is a short questionnaire for the assessment of health-related quality of life (HRQOL) in patients with intermittent claudication (IC). The objective of this study was to translate the ICQ into German and to investigate the psychometric properties of the German ICQ version in patients with IC. Patients and methods: The original English version was translated using a forward-backward method. The resulting German version was reviewed by the author of the original version and an experienced clinician. Finally, it was tested for clarity with 5 German patients with IC. A sample of 81 patients were administered the German ICQ. The sample consisted of 58.0 % male patients with a median age of 71 years and a median IC duration of 36 months. Test of feasibility included completeness of questionnaires, completion time, and ratings of clarity, length and relevance. Reliability was assessed through a retest in 13 patients at 14 days, and analysis of Cronbach’s alpha for internal consistency. Construct validity was investigated using principal component analysis. Concurrent validity was assessed by correlating the ICQ scores with the Short Form 36 Health Survey (SF-36) as well as clinical measures. Results: The ICQ was completely filled in by 73 subjects (90.1 %) with an average completion time of 6.3 minutes. Cronbach’s alpha coefficient reached 0.75. Intra-class correlation for test-retest reliability was r = 0.88. Principal component analysis resulted in a 3 factor solution. The first factor explained 51.5 of the total variation and all items had loadings of at least 0.65 on it. The ICQ was significantly associated with the SF-36 and treadmill-walking distances whereas no association was found for resting ABPI. Conclusions: The German version of the ICQ demonstrated good feasibility, satisfactory reliability and good validity. Responsiveness should be investigated in further validation studies.

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