scholarly journals Optimized Detection of Plasmodium falciparum Topoisomerase I Enzyme Activity in a Complex Biological Sample by the Use of Molecular Beacons

Sensors ◽  
2016 ◽  
Vol 16 (11) ◽  
pp. 1916 ◽  
Author(s):  
Asger Givskov ◽  
Emil Kristoffersen ◽  
Kamilla Vandsø ◽  
Yi-Ping Ho ◽  
Magnus Stougaard ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Enzyme Activity ◽  
Biological Sample ◽  
Topoisomerase I ◽  
Molecular Beacons ◽  
Complex Biological Sample

A comparative study of phosphopeptide-selective techniques for a sub-proteome of a complex biological sample

2016 ◽  
Vol 408 (9) ◽  
pp. 2347-2356 ◽  
Author(s):  
Malin Källsten ◽  
Jonas Bergquist ◽  
Hongxing Zhao ◽  
Anne Konzer ◽  
Sara Bergström Lind
Keyword(s):  
Comparative Study ◽  
Biological Sample ◽  
Complex Biological Sample

scholarly journals The enzymes of the glycolytic pathway in erythrocytes infected with Plasmodium falciparum malaria parasites

Blood ◽  
1988 ◽  
Vol 72 (6) ◽  
pp. 1922-1925 ◽  
Author(s):  
EF Jr Roth ◽  
MC Calvin ◽  
I Max-Audit ◽  
J Rosa ◽  
R Rosa
Keyword(s):  
Plasmodium Falciparum ◽  
Enzyme Activity ◽  
Adenylate Kinase ◽  
Genetic Basis ◽  
Glucose Consumption ◽  
Fold Increase ◽  
Plasmodium Falciparum Malaria ◽  
Red Cells ◽  
Glycolytic Pathway ◽  
Glucose 6 Phosphate Dehydrogenase

Abstract Enzymes of the glycolytic pathway as well as some ancillary enzymes were studied in normal red cells parasitized with Plasmodium falciparum in culture at varying parasitemias as well as in isolated parasites. The levels of all enzymes except diphosphoglycerate mutase, glucose-6- phosphate dehydrogenase, and adenylate kinase were elevated. Extreme elevations of hexokinase, aldolase, enolase, pyruvate kinase, and adenosine deaminase concentrations were noted. In most cases, electrophoretically distinct bands of enzyme activity were also seen. These findings partly explain the previously noted 50- to 100-fold increase in glucose consumption of infected red cells and suggest that further knowledge of these parasite enzymes and their genetic basis may aid both in designing new chemotherapy and in understanding the evolution of these parasites.

scholarly journals Rapid Detection of a Molecular Marker for Chloroquine-Resistant Falciparum Malaria

2002 ◽  
Vol 46 (8) ◽  
pp. 2684-2686 ◽  
Author(s):  
Rémy Durand ◽  
Virginie Huart ◽  
Sayeh Jafari ◽  
Jacques Le Bras
Keyword(s):  
Plasmodium Falciparum ◽  
Restriction Fragment Length Polymorphism ◽  
Molecular Marker ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Rapid Detection ◽  
Fragment Length Polymorphism ◽  
Molecular Beacons ◽  
Chloroquine Resistance ◽  
Restriction Fragment Length

ABSTRACT A PCR-based technique using molecular beacons was developed to detect the chloroquine resistance-associated pfcrt K76T point mutation in Plasmodium falciparum. One hundred thirty African clinical isolates were tested by the new method in comparison with the PCR-restriction fragment length polymorphism method. This rapid and inexpensive genomic assay could expand the possibilities for monitoring chloroquine resistance.

scholarly journals Theoretical and Experimental Studies of New Modified Isoflavonoids as Potential Inhibitors of Topoisomerase I from Plasmodium falciparum

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e91191 ◽  
Author(s):  
Wilian A. Cortopassi ◽  
Julia Penna-Coutinho ◽  
Anna C. C. Aguiar ◽  
André S. Pimentel ◽  
Camilla D. Buarque ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Topoisomerase I ◽  
Experimental Studies ◽  
Potential Inhibitors

scholarly journals DNA Topoisomerase I Domain Interactions Impact Enzyme Activity and Sensitivity to Camptothecin

2015 ◽  
Vol 290 (19) ◽  
pp. 12068-12078 ◽  
Author(s):  
Christine M. Wright ◽  
Marié van der Merwe ◽  
Amanda H. DeBrot ◽  
Mary-Ann Bjornsti
Keyword(s):  
Enzyme Activity ◽  
Topoisomerase I ◽  
Dna Topoisomerase I ◽  
Dna Topoisomerase ◽  
Domain Interactions
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