scholarly journals Continuous-Wave Stimulated Emission Depletion Microscope for Imaging Actin Cytoskeleton in Fixed and Live Cells

Sensors ◽  
2015 ◽  
Vol 15 (9) ◽  
pp. 24178-24190 ◽  
Author(s):  
Bhanu Neupane ◽  
Tao Jin ◽  
Liliana Mellor ◽  
Elizabeth Loboa ◽  
Frances Ligler ◽  
...  
Keyword(s):  
Actin Cytoskeleton ◽  
Continuous Wave ◽  
Stimulated Emission ◽  
Live Cells ◽  
Stimulated Emission Depletion

Superresolution imaging of telomeres with continuous wave stimulated emission depletion (STED) microscope

2016 ◽  
Vol 59 (11) ◽  
pp. 1519-1524 ◽  
Author(s):  
Shaopeng Wang ◽  
Suhui Deng ◽  
Xiaoqing Cai ◽  
Shangguo Hou ◽  
Jiajun Li ◽  
...  
Keyword(s):  
Continuous Wave ◽  
Stimulated Emission ◽  
Superresolution Imaging ◽  
Stimulated Emission Depletion

scholarly journals Three-Dimensional Stimulated Emission Depletion Microscopy of Nitrogen-Vacancy Centers in Diamond Using Continuous-Wave Light

Nano Letters ◽  
2009 ◽  
Vol 9 (9) ◽  
pp. 3323-3329 ◽  
Author(s):  
Kyu Young Han ◽  
Katrin I. Willig ◽  
Eva Rittweger ◽  
Fedor Jelezko ◽  
Christian Eggeling ◽  
...  
Keyword(s):  
Continuous Wave ◽  
Three Dimensional ◽  
Stimulated Emission ◽  
Nitrogen Vacancy ◽  
Nitrogen Vacancy Centers ◽  
Stimulated Emission Depletion ◽  
Continuous Wave Light

scholarly journals Low-Power Two-Color Stimulated Emission Depletion Microscopy for Live Cell Imaging

Biosensors ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 330
Author(s):  
Jia Zhang ◽  
Xinwei Gao ◽  
Luwei Wang ◽  
Yong Guo ◽  
Yinru Zhu ◽  
...  
Keyword(s):  
Low Power ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Super Resolution ◽  
Live Cell ◽  
Stimulated Emission ◽  
Live Cells ◽  
Sted Microscopy ◽  
Resolution Imaging ◽  
Stimulated Emission Depletion

Stimulated emission depletion (STED) microscopy is a typical laser-scanning super-resolution imaging technology, the emergence of which has opened a new research window for studying the dynamic processes of live biological samples on a nanometer scale. According to the characteristics of STED, a high depletion power is required to obtain a high resolution. However, a high laser power can induce severe phototoxicity and photobleaching, which limits the applications for live cell imaging, especially in two-color STED super-resolution imaging. Therefore, we developed a low-power two-color STED super-resolution microscope with a single supercontinuum white-light laser. Using this system, we achieved low-power two-color super-resolution imaging based on digital enhancement technology. Lateral resolutions of 109 and 78 nm were obtained for mitochondria and microtubules in live cells, respectively, with 0.8 mW depletion power. These results highlight the great potential of the novel digitally enhanced two-color STED microscopy for long-term dynamic imaging of live cells.

scholarly journals Localization STED (LocSTED) microscopy with 15 nm resolution

Nanophotonics ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 783-792 ◽  
Author(s):  
Sujitha Puthukodan ◽  
Eljesa Murtezi ◽  
Jaroslaw Jacak ◽  
Thomas A. Klar
Keyword(s):  
Single Molecule ◽  
Continuous Wave ◽  
Single Molecules ◽  
Cost Effective ◽  
Stimulated Emission ◽  
Localization Microscopy ◽  
Stimulated Emission Depletion ◽  
Single Molecule Localization Microscopy

AbstractWe present localization with stimulated emission depletion (LocSTED) microscopy, a combination of STED and single-molecule localization microscopy (SMLM). We use the simplest form of a STED microscope that is cost effective and synchronization free, comprising continuous wave (CW) lasers for both excitation and depletion. By utilizing the reversible blinking of fluorophores, single molecules of Alexa 555 are localized down to ~5 nm. Imaging fluorescently labeled proteins attached to nanoanchors structured by STED lithography shows that LocSTED microscopy can resolve molecules with a resolution of at least 15 nm, substantially improving the classical resolution of a CW STED microscope of about 60 nm. LocSTED microscopy also allows estimating the total number of proteins attached on a single nanoanchor.

scholarly journals A shape-switch-block method for confocal light-sheet microscopy with sectioned Bessel beams and stimulated emission depletion

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Luis Köbele ◽  
Alexander Rohrbach
Keyword(s):  
Continuous Wave ◽  
Imaging Techniques ◽  
Optical Resolution ◽  
Sheet Thickness ◽  
Light Sheet ◽  
Stimulated Emission ◽  
Bessel Beams ◽  
Isotropic Resolution ◽  
Light Sheet Microscopy ◽  
Stimulated Emission Depletion

Abstract Microscopy seeks to simultaneously maximize optical resolution, contrast, speed, volume size, and probe tolerability, which is possible by combining different complementary imaging techniques with their specific strengths. Here, we show how to combine three physical concepts to increase resolution and contrast in light-sheet microscopy by making the effective light-sheet thinner through phase shaping, fluorophores-switching, and dynamic blocking of fluorescence. This shape-switch-block principle is realized by illumination with two holographically shaped, sectioned Bessel beams. Second, by switching off fluorophores in the proximity of the excitation center using continuous-wave stimulated emission depletion (STED). And third, by blocking fluorescence outside the switching region by confocal line detection. Thereby, we reduce the light-sheet thickness by 35%, achieving an isotropic resolution with beads in a 300 × 70 × 50 µm³ volume. Without STED, we obtain 0.37 µm resolution in cell clusters at improved sectioning and penetration depth. The shape-switch-block concept promises high potential, also for other microscopy techniques.

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