scholarly journals Potential Use of Lactic Acid Bacteria with Pathogen Inhibitory Capacity as a Biopreservative Agent for Chorizo

Processes ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1582
Author(s):  
Ruby Stella Lucumi-Banguero ◽  
Cristina Ramírez-Toro ◽  
German A. Bolívar

The biopreservation of meat products is of great interest due to the demand for products with low or minimal chemical additives. Lactic acid bacteria (LAB) have been used as protective cultures for many centuries. The objective of this work was to characterize 10 native LAB isolated from meat masses with biopreservative potential for meat products. The isolates were subjected to viability tests with different concentrations of NaCl, nitrite, and nitrate salts, pHs, and temperature conditions. Antibiotic resistance and type of lactic acid isomer were tested. In addition, the isolates were tested against seven pathogens, and inhibitory substances were identified by diffusion in agar wells. Finally, two isolates, Lb. plantarum (SB17) and Lb. sakei (SB3) were tested as protective cultures of chorizo in a model. As a result, the viability at different concentrations of NaCl and nitrate and nitrate salts were obtained. pH and temperature exerted a negative effect on the growth of some of the isolates. Pathogens were inhibited mainly by the presence of organic acids; P. aurius was the most susceptible, and S. typhimurium and S. marcescens were the most resistant. The strains SB17 and SB3 had similar effects on chorizo, and time exerted a deleterious effect on microbiological quality and pH. The results indicated that the 10 isolates show promising characteristics for the preservation of cooked meat products, with the strain Lb. plantarum (SB17) being the most promising.

1990 ◽  
Vol 53 (9) ◽  
pp. 793-794 ◽  
Author(s):  
HANNU J. KORKEALA ◽  
PIA M. MÄKELÄ ◽  
HANNU L. SUOMINEN

The minimum, optimum, and maximum growth temperatures of ropy slime-producing lactic acid bacteria able to spoil vacuum-packed cooked meat products were determined on MRS-agar with temperature-gradient incubator GradiplateR W10. The minimum growth temperatures of slime-producing lactobacilli and Leuconostoc mesenteroides strain D1 were below −1°C and 4°C, respectively. The low minimum growth temperature allows these bacteria to compete with other bacteria in meat processing plants and in meat products causing ropiness problems. The maximum growth temperatures varied between 36.6–39.8°C. The maximum growth temperature of lactobacilli seemed to be an unstable character. Single lactobacilli colonies were able to grow above the actual maximum growth temperature, which is determined as the edge of continuous growth of the bacteria. The significance of this phenomenon needs further study.


1997 ◽  
Vol 44 (12) ◽  
pp. 855-861 ◽  
Author(s):  
Takashi SAMESHIMA ◽  
Kazuko TAKESHITA ◽  
Masanobu AKIMOTO ◽  
Hiroyuki YAMANAKA ◽  
Tameo MIKI ◽  
...  

1998 ◽  
Vol 4 (3) ◽  
pp. 141-158 ◽  
Author(s):  
M.T. Aymerich ◽  
M. Hugas ◽  
J.M. Monfort

Meat consumption is of great economical importance. Several lactic acid bacteria associated with meat products are important natural bacteriocin producers. Bacteriocins are proteinaceous antag onistic substances considered to be important in the control of spoilage and pathogenic microor ganisms. This review aims to present the current state of the art in terms of bacteriocinogenic lactic acid bacteria associated with fresh and fermented meat products, describe the biochemical and genetic characteristics of their bacteriocins and the potential use of bacteriocins production of meat products.


Nacameh ◽  
2007 ◽  
Vol 1 (2) ◽  
pp. 87-96
Author(s):  
Maria de Lourdes Perez-Chabela ◽  
Norma Leticia Ramirez-Chavarin ◽  

2021 ◽  
Vol 27 (5) ◽  
pp. 759-768
Author(s):  
Shiro Takeda ◽  
Jumpei Uchiyama ◽  
Kazutoshi Sugita ◽  
Hirofumi Enomoto ◽  
Abdulatef M Ahhmed ◽  
...  

2020 ◽  
Vol 1 (2) ◽  
pp. 72-82
Author(s):  
Dyah Triasih ◽  
Y. O. Linata ◽  
M. Hilmi ◽  
A. U. Prastujati ◽  
S. Ton

Abstrak Objectif. Salami adalah salah satu produk asal daging yang dibuat dengan penambahan kultur bakteri yang kemudian difermentasi selama ±48 jam. Penelitian ini bertujuan untuk mengetahui kosentrasi penggunaan  tepung umbi uwi (Dioscorea spp.) sebagai prebiotik terhadap kualitas kimia dan kualitas mikrobiologi yang meliputi uji kadar pati, uji pH, uji TAT, dan uji BAL salami daging ayam broiler. Materi dan Metode. Bahan yang digunakan dalam penelitian ini adalah ayam broiler yang ditambahkan tepung uwi dengan kosentrasi yang berbeda. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan pola searah yang terdiri dari 4 perlakuan yaitu dengan konsentasi 0%, 5%, 15%, dan 25%, masing-masing perlakuan terdiri dari 3 ulangan sehingga diperoleh 12 kali ulangan atau 12 unit percobaan. Hasil. Hasil penelitian menunjukkan konsentrasi 5% (P1) sampai 25% (P3) mampu meningkatkan viabilitas bakteri asam laktat sebesar 6,58% sampai 6,94% log CFU/g serta dapat meningkatkan nilai TAT. Penambahan tepung umbi uwi dengan konsentrasi 5% (P1) sampai 25% (P3) juga dapat menurunkan kadar pati dan pH dari salami. Kesimpulan. Kesimpulannya penambahan tepung uwi dengan kosentrasi yang berbeda mampu mempengaruhi kualitas salami daging ayam broiler. Conclusion. The conclusion is the addition of uwi flour with different concentrations can affect the quality of broiler chicken salami. Abstrack Objectif. Salami is one of the meat products made with the addition of bacterial culture, fermenting for ± 48 hours. This study aims to determine the concentration of the use of Uwi tuber flour (Dioscorea spp.) As a prebiotic on chemical quality and microbiological quality, which includes starch test, pH test, TAT test, and BAL test for broiler chicken salami. Material and Methods. The material used in this research is broiler chicken, which added with uwi flour with different concentrations. This study uses a completely randomized design (CRD) with a unidirectional pattern consisting of 4 treatments, with a concentration of 0%, 5%, 15%, and 25%. Each treatment consists of 3 replications so that 12 replicas or 12 units of trials obtained. Results. The results showed a concentration of 5% (P1) to 25% (P3) could increase the viability of lactic acid bacteria by 6.58% to 6.94% log CFU / g and could increase the TAT value. The addition of Uwi bulbs with a 5% (P1) to 25% (P3) can also reduce starch levels and pH from salami. Conclusion. The conclusion is the addition of uwi flour with different concentrations can affect the quality of broiler chicken salami.


2003 ◽  
Vol 69 (6) ◽  
pp. 3668-3671 ◽  
Author(s):  
Yoshikatsu Hamasaki ◽  
Mitsuko Ayaki ◽  
Hidetaka Fuchu ◽  
Masaaki Sugiyama ◽  
Hidetoshi Morita

ABSTRACT Three kinds of lactic acid bacteria were isolated from spoiling cooked meat products stored below 10�C. They were identified as Leuconostoc mesenteroides subsp. mesenteroides, Lactococcus lactis subsp. lactis, and Leuconostoc citreum. All three strains grew well in MRS broth at 10�C. In particular, L. mesenteroides subsp. mesenteroides and L. citreum grew even at 4�C, and their doubling times were 23.6 and 51.5 h, respectively. On the other hand, although the bacteria were initially below the detection limit (<10 CFU/g) in model cooked meat products, the bacterial counts increased to 108 CFU/g at 10�C after 7 to 12 days.


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