scholarly journals Phytosynthesis of Copper Nanoparticles Using Extracts of Spices and Their Antibacterial Properties

Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1341
Author(s):  
Gayathri Vijayakumar ◽  
Hindhuja Kesavan ◽  
Anisha Kannan ◽  
Dhanalakshmi Arulanandam ◽  
Jeong Hee Kim ◽  
...  
Keyword(s):  
Functional Groups ◽  
Copper Nanoparticles ◽  
Antibacterial Properties ◽  
Growth Zone ◽  
Antibacterial Activities ◽  
Sem Analysis ◽  
Microbial Diseases ◽  
Star Anise ◽  
Myristica Fragrans ◽  
Vis Spectroscopy

To prevent microbial growth, chemical solvents are typically utilized. However, chemical solvents are hazardous to human health with low antimicrobial effects. Metallic-element (such as copper, silver, and gold) nanoparticles have many applications in biotechnology and biomedicine. Copper nanoparticles (CuNPs) are efficient owing to their antimicrobial, anti-inflammatory, and anti-proliferative properties. The objective of this study was to perform biogenic synthesis of copper nanoparticles using three different spices (star anise, seed of Illicium verum; nutmeg, seed of Myristica fragrans; and mace, membrane covering the seed of Myristica fragrans) and determine their antibacterial properties. CuNPs of spices were prepared by dissolving copper sulfate in the respective plant extract. They were then characterized by UV-Vis spectroscopy, FTIR, GC-MS, EDAX, and SEM analysis. Results of UV-Vis spectroscopy showed the maximum absorbance peak at 350 nm. SEM analysis showed that the sizes of these CuNPs were in the range of 150–200 nm. EDAX analysis confirmed the presence of copper and oxygen and revealed that copper existed in an oxidized form. FTIR spectroscopy showed the presence of different functional groups in these synthesized nanoparticles. GC-MS analysis revealed compounds such as Anethole D-limonene, heptadecanoic acid, 16-methyl-, methyl ester, myristene, methyl eugenol, and methyl stearate, indicating the presence of functional groups. The antibacterial activities of the three extracts from spices were analyzed using growth zone inhibition and TLC-bioautography methods. The results showed that star anise spice extract had the highest antibacterial activity. These results indicate that such CuNPs phyto-formulated with spice extracts having antibacterial properties could be used as potential therapeutics for microbial diseases.

scholarly journals Interaction of Silver Nanoparticles with Serum Proteins Affects Their Antimicrobial ActivityIn Vivo

2013 ◽  
Vol 57 (10) ◽  
pp. 4945-4955 ◽  
Author(s):  
Divya Prakash Gnanadhas ◽  
Midhun Ben Thomas ◽  
Rony Thomas ◽  
Ashok M. Raichur ◽  
Dipshikha Chakravortty
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Serum Albumin ◽  
Serum Proteins ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Resistant Bacteria ◽  
Human Society ◽  
Vis Spectroscopy

ABSTRACTThe emergence of multidrug-resistant bacteria is a global threat for human society. There exist recorded data that silver was used as an antimicrobial agent by the ancient Greeks and Romans during the 8th century. Silver nanoparticles (AgNPs) are of potential interest because of their effective antibacterial and antiviral activities, with minimal cytotoxic effects on the cells. However, very few reports have shown the usage of AgNPs for antibacterial therapyin vivo. In this study, we deciphered the importance of the chosen methods for synthesis and capping of AgNPs for their improved activityin vivo. The interaction of AgNPs with serum albumin has a significant effect on their antibacterial activity. It was observed that uncapped AgNPs exhibited no antibacterial activity in the presence of serum proteins, due to the interaction with bovine serum albumin (BSA), which was confirmed by UV-Vis spectroscopy. However, capped AgNPs [with citrate or poly(vinylpyrrolidone)] exhibited antibacterial properties due to minimized interactions with serum proteins. The damage in the bacterial membrane was assessed by flow cytometry, which also showed that only capped AgNPs exhibited antibacterial properties, even in the presence of BSA. In order to understand thein vivorelevance of the antibacterial activities of different AgNPs, a murine salmonellosis model was used. It was conclusively proved that AgNPs capped with citrate or PVP exhibited significant antibacterial activitiesin vivoagainstSalmonellainfection compared to uncapped AgNPs. These results clearly demonstrate the importance of capping agents and the synthesis method for AgNPs in their use as antimicrobial agents for therapeutic purposes.

scholarly journals Starch-Capped Silver Nanoparticles Impregnated into Propylamine-Substituted PVA Films with Improved Antibacterial and Mechanical Properties for Wound-Bandage Applications

Polymers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2112 ◽  
Author(s):  
Mudassir Iqbal ◽  
Hadia Zafar ◽  
Azhar Mahmood ◽  
Muhammad Bilal Khan Niazi ◽  
Muhammad Waqar Aslam
Keyword(s):  
Silver Nanoparticles ◽  
Wound Dressing ◽  
Antibacterial Properties ◽  
Antimicrobial Activities ◽  
Sem Analysis ◽  
Ag Nps ◽  
Blend Film ◽  
Physical Strength ◽  
Vis Spectroscopy ◽  
Average Size

This research endeavor aims to develop polyvinyl alcohol (PVA) based films capable of blends with silver nanoparticles (Ag–NPs) for improved antibacterial properties and good mechanical strength to widen its scope in the field of wound dressing and bandages. This study reports synthesis of propylamine-substituted PVA (PA–PVA), Ag–NPs via chemical and green methods (starch capping) and their blended films in various proportions. Employment of starch-capped Ag–NPs as nanofillers into PVA films has substantially improved the above-mentioned properties in the ensuing nanocomposites. Synthesis of PA–PVA, starch-capped Ag–NPs and blended films were well corroborated with UV/Vis spectroscopy, FTIR, NMR, XRD and SEM analysis. Synthesized Ag–NPs were of particle shape and have an average size 20 nm and 40 nm via green and chemical synthesis, respectively. The successful blending of Ag–NPs was yielded up to five weight per weight into PA–PVA film as beyond this self-agglomeration of Ag–NPs was observed. Antibacterial assay has shown good antimicrobial activities by five weight per weight Ag–NPs(G)-encapsulated into PA–PVA blended film, i.e., 13 mm zone inhibition against Escherichia coli and 11 mm zone inhibition against Staphylococcus aureus. Physical strength was measured in the terms of young’s modulus via tensile stress–strain curves of blended films. The five weight per weight Ag–NPs(G)/PA–PVA blend film showed maximum tensile strength 168.2 MPa while three weight per weight Ag–NPs(G)/PVA blend film showed highest values for ultimate strain 297.0%. Ag–NPs embedment into PA–PVA was resulted in strong and ductile film blend than pristine PA–PVA film due to an increase in hydrogen bonding. These good results of five weight per weight Ag–NPs(G)/PA–PVA product make it a potent candidate for wound dressing application in physically active body areas.

Photocatalytic and antibacterial activities study of prepared self-cleaning nanostructure surfaces using synthesized and coated ZnO nanoparticles with Curcumin nanodispersion

2019 ◽  
Vol 234 (5) ◽  
pp. 307-328 ◽  
Author(s):  
Zahra Sayyar ◽  
Hoda Jafarizadeh Malmiri
Keyword(s):  
Photocatalytic Activity ◽  
Specific Surface ◽  
Band Gap ◽  
Surface Area ◽  
Specific Surface Area ◽  
Urbach Energy ◽  
Subcritical Water ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Vis Spectroscopy

Abstract Zinc oxide nanoparticles had been synthesized and encapsulated using Curcumin nanoemulsions, Zn(Cur)O NPs, under subcritical water conditions. The effects of temperature (120, 140 and 160 °C) and pH values of the reaction solution (4, 7 and 10) on the particle size, grain size, cristallinity, specific surface area, band gap, Urbach energy, morphology, photocatalytic activity and antibacterial properties of the prepared Zn(Cur)O NPs were evaluated using XRD, FT-IR, SEM, EDX and UV-Vis spectroscopy analysis. The obtained results indicate that the prepared spherical and rod shapes Zn(Cur)O NPs had a crystallite size distribution of 10–100 nm. Furthermore, the results reveal that most uniform Zn(Cur)O NPs with highest photocatalytic activity, quantum yield (0.161 mol·m−2 s−1), specific surface area (242 m2/g), minimum band gap (2.62 eV) and Urbach energy (0.125 meV) were formed at 160 °C and natural pH. The highest antibacterial activities against both Gram positive and negative bacteria strains, were achieved using the synthesized Zn(Cur)O at 160 °C and basic pH(10).

scholarly journals Study on Bactericidal Effect of Biosynthesized Silver Nanoparticles in Combination with Gentamicin and Ampicillin on Pseudomonas aeruginosa

Nano Hybrids ◽  
2013 ◽  
Vol 3 ◽  
pp. 37-49 ◽  
Author(s):  
Shweta Rajawat ◽  
M.S. Qureshi
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Antibacterial Properties ◽  
Direct Interaction ◽  
Antibacterial Activities ◽  
Bactericidal Effect ◽  
Bactericidal Properties ◽  
Vis Spectroscopy ◽  
Tea Extract

Silver nanoparticles are the most promising nanomaterial with antibacterial properties. Recent study of resistance to most potential antibiotics promotes research in the bactericidal activity of the silver nanoparticles. In this work, the effect of biosynthesized silver nanoparticles, in combination with gentamicin and ampicillin, on Pseudomonas Aeruginosa bacteria has been studied. Pseudomonas Aeruginosa is a common bacterium that can cause infections which are generalized as inflammation and sepsis. The results show that the bactericidal properties of the nanoparticles depends on the size of the as-synthesized silver nanoparticles as nanoparticles of diameter ~120 nm only have a direct interaction with the bacteria. It is observed that the antibacterial activities of antibiotics increase in the presence of AgNPs against test strains. Silver nanoparticles were synthesized elctrolytically using silver wire of 99% purity as anode and carbon rod wrapped with LDPE as cathode. 0.01 N Silver nitrate was used as an electrolyte. The process is termed as biosynthesis, because tea extract was used used as the capping agent which is also a very mild reducing agent. The polyphenols theaflavins and thearubigins, present in tea perform the role of stabilizing or capping agents due to their bulky and steric nature. A brown colored colloidal solution of silver nanoparticles is obtained. The as-synthesized silver nanoparticles were characterized using XRD, TEM and UV-Vis spectroscopy.

scholarly journals Biodegradable Polymer Films with a Natural Antibacterial Extract as Novel Periodontal Barrier Membranes

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Zehra Betul Ahi ◽  
Nergis Zeynep Renkler ◽  
Mine Gul Seker ◽  
Kadriye Tuzlakoglu
Keyword(s):  
Low Cost ◽  
Composite Films ◽  
Antibacterial Properties ◽  
Composite Membranes ◽  
Antibacterial Activities ◽  
Sem Analysis ◽  
Ease Of Use ◽  
Casting Method ◽  
Barrier Membranes ◽  
Biodegradable Composite

Biodegradable composite membranes containing propolis were produced from PCL/PLLA blends using a simple and low-cost solvent casting method, and subsequently their physicochemical, mechanical, and antibacterial properties were characterized. SEM analysis revealed that the addition of propolis has created honeycomb-like structures on the film surfaces. The flexibility of the films increased in the presence of propolis, which may provide ease of use during application. Propolis disrupted the organized structure of both polymers at the molecular level and caused decreases in the melting points. The films with propolis showed faster degradation in physiological conditions due to this molecular disruption. Moreover, the PLLA/PCL/propolis composite films exhibited remarkable antibacterial activities againstS. aureus. Collectively, the data suggest that the produced films might be used as an alternative to exiting barrier membranes in guided tissue regeneration.

scholarly journals GREEN SYNTHESIS OF SILVER NANOPARTICLES FROM FRUIT RIND EXTRACT OF GARCINIA MANGOSTANA L. AND EVALUATION OF ANTIBACTERIAL PROPERTIES

2021 ◽  
Vol 21 (2) ◽  
Author(s):  
Manju Madhavan ◽  
Elsa Rose P.J. ◽  
Arunima Francis ◽  
Grace Maria Benny ◽  
Anila Wilson
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Wave Length ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Toxic Substances ◽  
Garcinia Mangostana ◽  
Vis Spectroscopy ◽  
Fruit Rind

Nanotechnology is an emerging area of science that involves the engineering of nano sized particles of various materials. Among the various nanoparticles, the silver nanoparticles are used in various applications due to their unique characters. The production of silver nanoparticles using a chemical method is harmful and produces toxic substances as by-products, so the aim of our study was to green synthesis silver nanoparticles using the fruit rind of Garcinia mangostana L. which is being discarded as a waste material. Aqueous rind extract of G. mangostana was prepared and synthesis of silver nanoparticles was analysed by UV-Vis spectrophotometer by optimizing various parameters like pH, time and concentration of extract. The nanoparticles were characterized using UV-Vis spectroscopy and the peak was obtained between the wave length of 410 - 433 nm in various treatments. The antibacterial activities of synthesized silver nanoparticles were tested against both gram negative (Pseudomonas) and gram positive (Staphylococcus) bacteria using the well diffusion method. The aqueous extract shows the remarkable zone of inhibition against Pseudomonas and Staphylococcus.

Geraniol and cinnamaldehyde as natural antibacterial additives for poly(lactic acid) and their plasticizing effects

2019 ◽  
Vol 40 (1) ◽  
pp. 38-48
Author(s):  
Mert Akgün ◽  
İhsan Başaran ◽  
Salih C. Suner ◽  
Ayhan Oral
Keyword(s):  
Lactic Acid ◽  
Antibacterial Activities ◽  
Mechanical Tests ◽  
Sem Analysis ◽  
Diffusion Method ◽  
Poly Lactic Acid ◽  
Scanning Calorimetry ◽  
Disk Diffusion Method ◽  
Vis Spectroscopy ◽  
The Matrix

Abstract The main goal of this study is to prepare antibacterial poly(lactic acid) (PLA) containing cinnamaldehyde and geraniol and to evaluate the antibacterial activity and assess the changes of physical properties of the PLA films. Cinnamaldehyde- and geraniol-incorporated (10%, 20%, 30%, and 50% v/w) PLA films were prepared via solution-casting. While preparing these films, plasticizers were not added to the matrix. Antibacterial activities of these films against Escherichia coli and Staphylococcus aureus were investigated by the disk diffusion method. Thermal degradation characteristics were analyzed via thermogravimetric analysis (TGA), glass transition, crystallization, and melting temperatures, and enthalpies of the films were determined from differential scanning calorimetry (DSC) scans. Tensile strength and elongation-at-break values of neat PLA and antibacterial-compound-containing films were evaluated and compared after the mechanical tests. Moreover, the changes in the polymer morphology were observed by SEM analysis, and opacity of the films was determined by UV-vis spectroscopy. Our results showed that both compounds provided antibacterial effect to the PLA, with cinnamaldehyde being more effective than geraniol. Moreover, plasticization effects of the compounds were confirmed by DSC analysis.

Effect of doping on structural and optical properties of ZnO nanoparticles: study of antibacterial properties

2014 ◽  
Vol 32 (2) ◽  
pp. 130-135 ◽  
Author(s):  
P. Maddahi ◽  
N. Shahtahmasebi ◽  
A. Kompany ◽  
M. Mashreghi ◽  
S. Safaee ◽  
...  
Keyword(s):  
Optical Properties ◽  
Band Gap ◽  
Zno Nanoparticles ◽  
Sol Gel ◽  
Antibacterial Properties ◽  
Phase Segregation ◽  
Hexagonal Wurtzite Structure ◽  
Antibacterial Activities ◽  
Structure Morphology ◽  
Vis Spectroscopy

AbstractSol-gel method was successfully used for synthesis of ZnO nanoparticles doped with 10 % Mg or Cu. The structure, morphology and optical properties of the prepared nanoparticles were studied as a function of doping content. The synthesized ZnO:(Mg/Cu) samples were characterized using XRD, TEM, FTIR and UV-Vis spectroscopy techniques. The samples show hexagonal wurtzite structure, and the phase segregation takes place for Cu doping. Optical studies revealed that Mg doping increases the energy band gap while Cu incorporation results in decrease of the band gap. The antibacterial activities of the nanoparticles were tested against Escherichia coli (Gram negative bacteria) cultures. It was found that both pure and doped ZnO nanosuspensions show good antibacterial activity which increases with copper doping, and slightly decreases with adding Mg.

Medicinal Plants: Antibacterial Effects and Chemical Composition of Essential Oil of Foeniculum vulgare

2017 ◽  
Vol 8 (01) ◽  
Author(s):  
Azadeh Foroughi ◽  
Pouya Pournaghi ◽  
Fariba Najafi ◽  
Akram Zangeneh ◽  
Mohammad Mahdi Zangeneh ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Medicinal Plants ◽  
Essential Oil ◽  
Antibacterial Properties ◽  
Screen Test ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Resistant Bacteria ◽  
Antibacterial Effects ◽  
Foeniculum Vulgare

Medicinal plants are considered modern resources for producing agents that could act as alternatives to antibiotics in demeanor of antibiotic-resistant bacteria. The aim of the study was to evaluate the chemical composition and antibacterial activities of essential oil of Foeniculum vulgare (FV) against Pseudomonas aeruginosa and Bacillus subtilis. Gas chromatography mass spectrometry was done to specify chemical composion. As a screen test to detect antibacterial properties of the essential oil, agar disk and agar well diffusion methods were employed. Macrobroth tube test was performed to determinate MIC. The results indicated that the most substance found in FV essential oil was Trans-anethole (47.41 %), also the essential oil of FV with 0.007 g/ml concentration has prevented P. aeruginosa and with 0.002 g/ml concentration has prevented B. subtilis from the growth. Thus, the research represents the antibacterial effects of the medical herb on test P. aeruginosa and B. subtilis. We believe that the article provide support to the antibacterial properties of the essential oil. The results indicate the fact that the essential oil from the plant can be useful as medicinal or preservatives composition.

scholarly journals In Vitro Anti-Biofilm and Antibacterial Properties of Streptococcus downii sp. nov.

2021 ◽  
Vol 9 (2) ◽  
pp. 450
Author(s):  
Maigualida Cuenca ◽  
María Carmen Sánchez ◽  
Pedro Diz ◽  
Lucía Martínez-Lamas ◽  
Maximiliano Álvarez ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Quantitative Polymerase Chain Reaction ◽  
Bacterial Load ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Oral Bacteria ◽  
Periodontal Pathogens ◽  
Biofilm Model ◽  
Biofilm Development

The aim of this study was to evaluate the potential anti-biofilm and antibacterial activities of Streptococcus downii sp. nov. To test anti-biofilm properties, Streptococcus mutans, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans were grown in a biofilm model in the presence or not of S. downii sp. nov. for up to 120 h. For the potential antibacterial activity, 24 h-biofilms were exposed to S. downii sp. nov for 24 and 48 h. Biofilms structures and bacterial viability were studied by microscopy, and the effect in bacterial load by quantitative polymerase chain reaction. A generalized linear model was constructed, and results were considered as statistically significant at p < 0.05. The presence of S. downii sp. nov. during biofilm development did not affect the structure of the community, but an anti-biofilm effect against S. mutans was observed (p < 0.001, after 96 and 120 h). For antibacterial activity, after 24 h of exposure to S. downii sp. nov., counts of S. mutans (p = 0.019) and A. actinomycetemcomitans (p = 0.020) were significantly reduced in well-structured biofilms. Although moderate, anti-biofilm and antibacterial activities of S. downii sp. nov. against oral bacteria, including some periodontal pathogens, were demonstrated in an in vitro biofilm model.

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