scholarly journals Characterization of Phenolics in Rejected Kiwifruit and Their Antioxidant Potential

Processes ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 781
Author(s):  
Chen Zhu ◽  
Osbert Chou ◽  
Fung Ying Lee ◽  
Zening Wang ◽  
Colin J. Barrow ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Economic Value ◽  
Antioxidant Potential ◽  
Actinidia Deliciosa ◽  
Phenolic Profile ◽  
Diverse Range ◽  
Three Samples ◽  
And Storage

Kiwifruit hold significant nutritional value and are a good source of antioxidants due to their diverse range of bioactive compounds. Kiwifruit waste is generated throughout the food supply chain, particularly during transportation and storage. Kiwifruit rejected from the retail market due to unfavorable appearance still possess potential economic value as kiwifruit are abundant in phenolic compounds. The present work studied the phenolic profile and antioxidant potential of rejected kiwifruit, including SunGold (Actinidia chinensis), Hayward (Actinidia deliciosa), and round organic Hayward (Actinidia deliciosa). Regarding phenolics estimation, SunGold possessed the highest TPC (0.72 ± 0.01 mg GAE/g), while Hayward exhibited the highest TFC (0.05 ± 0.09 mg QE/g). In antioxidant assays, SunGold showed the highest antioxidant activities in DPPH (0.31 ± 0.35 mg AAE/g), FRAP (0.48 ± 0.04 mg AAE/g), ABTS (0.69 ± 0.07 mg AAE/g), •OH-RSA (0.07 ± 0.03 mg AAE/g) assays, and FICA (0.19 ± 0.07 mg EDTA/g), whereas Hayward showed the highest RPA (0.09 ± 0.02 mg AAE/g) and TAC (0.57 ± 0.04 mg AAE/g). Separation and characterization of phenolics were conducted using LC-ESI-QTOF-MS/MS. A total of 97 phenolics were tentatively characterized from rejected SunGold (71 phenolics), Hayward (55 phenolics), and round organic Hayward (9 phenolics). Hydroxycinnamic acids and flavonols were the most common phenolics characterized in the three samples. The quantitative analysis was conducted by HPLC-PDA and found that chlorogenic acid (23.98 ± 0.95 mg/g), catechin (23.24 ± 1.16 mg/g), and quercetin (24.59 ± 1.23 mg/g) were the most abundant phenolics present in the rejected kiwifruit samples. The notable presence of phenolic compounds and their corresponding antioxidant capacities indicate the potential value of rescuing rejected kiwifruit for further utilization and commercial exploitation.

scholarly journals Screening and Characterization of Phenolic Compounds and Their Antioxidant Capacity in Different Fruit Peels

Foods ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1206 ◽  
Author(s):  
Hafiz A. R. Suleria ◽  
Colin J. Barrow ◽  
Frank R. Dunshea
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Phenolic Acids ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Antioxidant Potential ◽  
Fruit Peel ◽  
Diverse Range ◽  
Mango Peel

Fruit peels have a diverse range of phytochemicals including carotenoids, vitamins, dietary fibres, and phenolic compounds, some with remarkable antioxidant properties. Nevertheless, the comprehensive screening and characterization of the complex array of phenolic compounds in different fruit peels is limited. This study aimed to determine the polyphenol content and their antioxidant potential in twenty different fruit peel samples in an ethanolic extraction, including their comprehensive characterization and quantification using the LC-MS/MS and HPLC. The obtained results showed that the mango peel exhibited the highest phenolic content for TPC (27.51 ± 0.63 mg GAE/g) and TFC (1.75 ± 0.08 mg QE/g), while the TTC (9.01 ± 0.20 mg CE/g) was slightly higher in the avocado peel than mango peel (8.99 ± 0.13 mg CE/g). In terms of antioxidant potential, the grapefruit peel had the highest radical scavenging capacities for the DPPH (9.17 ± 0.19 mg AAE/g), ABTS (10.79 ± 0.56 mg AAE/g), ferric reducing capacity in FRAP (9.22 ± 0.25 mg AA/g), and total antioxidant capacity, TAC (8.77 ± 0.34 mg AAE/g) compared to other fruit peel samples. The application of LC-ESI-QTOF-MS/MS tentatively identified and characterized a total of 176 phenolics, including phenolic acids (49), flavonoids (86), lignans (11), stilbene (5) and other polyphenols (25) in all twenty peel samples. From HPLC-PDA quantification, the mango peel sample showed significantly higher phenolic content, particularly for phenolic acids (gallic acid, 14.5 ± 0.4 mg/g) and flavonoids (quercetin, 11.9 ± 0.4 mg/g), as compared to other fruit peel samples. These results highlight the importance of fruit peels as a potential source of polyphenols. This study provides supportive information for the utilization of different phenolic rich fruit peels as ingredients in food, feed, and nutraceutical products.

scholarly journals Phenolic Profile and Bioactive Potential of Stems and Seed Kernels of Sweet Cherry Fruit

Antioxidants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1295 ◽  
Author(s):  
Sílvia Afonso ◽  
Ivo Vaz Oliveira ◽  
Anne S. Meyer ◽  
Alfredo Aires ◽  
Maria José Saavedra ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Sweet Cherry ◽  
Antioxidant Activities ◽  
Antibacterial Activities ◽  
Gram Positive ◽  
Phenolic Profile ◽  
Bioactive Potential ◽  
Sweet Cherry Cultivars

Every year, large quantities of stems and pits are generated during sweet cherry processing, without any substantial use. Although stems are widely recognized by traditional medicine, detailed and feasible information about their bioactive composition or biological value is still scarce, as well as the characterization of kernels. Therefore, we conducted a study in which bioactivity potential of extracts from stems and kernels of four sweet cherry cultivars (Early Bigi (grown under net cover (C) and without net cover (NC)), Burlat, Lapins, and Van) were examined. The assays included antioxidant (by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene-linoleic acid bleaching assays), and antibacterial activities against important Gram negative and Gram positive bacterial human isolates. Profile and individual phenolic composition of each extract were determined by High-performance liquid chromatography (HPLC) analysis. Extracts from stems of cv. Lapins and kernels of Early Bigi NC presented high levels of total phenolics, flavonoids, ortho-diphenols and saponins. Excepting for cv. Early Bigi NC, major phenolic compounds identified in stems and kernels were sakuranetin and catechin, respectively. In cv. Early Bigi NC the most abundant compounds were ellagic acid for stems and protocatechuic acid for kernels. In all extracts, antioxidant activities showed a positive correlation with the increments in phenolic compounds. Antimicrobial activity assays showed that only stem’s extracts were capable of inhibiting the growth of Gram positive isolates. This new data is intended to provide new possibilities of valorization of these by-products and their valuable properties.

scholarly journals An Environmentally Friendly Practice Used in Olive Cultivation Capable of Increasing Commercial Interest in Waste Products from Oil Processing

Antioxidants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 466
Author(s):  
Irene Dini ◽  
Giulia Graziani ◽  
Francalisa Luisa Fedele ◽  
Andrea Sicari ◽  
Francesco Vinale ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
High Performance ◽  
Mevalonate Pathway ◽  
Total Concentration ◽  
Economic Value ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Waste Products ◽  
Vegetation Water

In the Rural Development Plan (2014–2020), the European Commission encouraged the conversion and supported the maintenance of organic farming. Organic olive oil (bioEVOO) production involves the use of environmentally sustainable fertilizers and the recycling of olive pomace (Pom) and olive vegetation waters (VW) to reduce the environmental impact of these wastes. An ecofriendly way to recycle olive wastes is to reuse them to extract bioactive compounds. In this study, the total phenolic compounds content, their profile and dosage, the antioxidant action in oil, pomace, and vegetation water was evaluated when the Trichoderma harzianum M10 was used as a biostimulant in agriculture. Two spectrophotometric tests (2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic) acid (ABTS)) evaluated the antioxidant potential of samples, a spectrophotometric method estimated total phenolic content, and an Ultra-High-Performance Liquid Chromatography (UHPLC)–Orbitrap method evaluated the phenolics profile. Our results showed that the biostimulation improved the antioxidant potential and the total concentration of phenolics in the bioEVOO and bio-pomace (bioPom) samples and mainly enhanced, among all classes of phenolic compounds, the production of the flavonoids and the secoiridoids. Moreover, they demonstrated the Trichoderma action in the mevalonate pathway to produce phenols for the first time. The decisive action of the Thricoderma on the production of phenolic compounds increases the economic value of the waste materials as a source of bioactive compounds useful for the pharmaceutical, cosmetic, and food industries.

scholarly journals In-depth phenolic characterization of iron gall inks by deconstructing representative Iberian recipes

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Natércia Teixeira ◽  
Paula Nabais ◽  
Victor de Freitas ◽  
João A. Lopes ◽  
Maria J. Melo
Keyword(s):  
Phenolic Compounds ◽  
Gum Arabic ◽  
White Wine ◽  
Molecular Structures ◽  
Phenolic Profile ◽  
Essential Information ◽  
History Of ◽  
Iron Gall ◽  
History Of Western Civilization

AbstractIron-gall ink is one of the most important inks in the history of western civilization. The deep black colour results from Fe3+ complexes with phenolic compounds available in gall extracts. Unfortunately, it induces the degradation of both ink and support over time. Furthermore, our knowledge of these complex molecular structures is limited. This work aims to overcome this gap, revealing essential information about the complex structures of these pigments and dyes that will create a breakthrough in the next generation of conservation treatments. It presents the first in-depth phenolic identification and quantification of extracts and inks, prepared with and without gum arabic (an essential additive in medieval recipes). Five representative Iberian recipes were selected and prepared. Their phenolic profile was analysed by HPLC–DAD and HPLC–ESI–MS, which revealed that the phenolic compounds present in higher concentration, in the gall extracts, are pentagalloylglucose and hexagalloylglucose (0.15 ± 0.01–32 ± 3 mg/mL), except for one recipe, in which gallic acid is the main phenolic. The influence of the ingredients is also discussed by deconstructing the recipes: extracts of additives as pomegranate peel and solvents used in the extraction of the galls (vinegar and white wine) were characterized.

scholarly journals Antioxidant Activities of Sonchus oleraceus L.

2021 ◽  
Author(s):  
◽  
Sundara Mudiyanselage Maheshini Rangika Mawalagedera
Keyword(s):  
Phenolic Compounds ◽  
Chlorogenic Acid ◽  
Hepg2 Cells ◽  
Antioxidant Activities ◽  
Antioxidant Potential ◽  
Gastrointestinal Digestion ◽  
Sonchus Oleraceus ◽  
In Vitro Gastrointestinal Digestion ◽  
Abiotic Stressors

<p>Supernumerary free radicals and other reactive species can cause oxidative damage in animal cells, potentially leading to non-infectious diseases. Diets rich in low molecular weight antioxidants (LMWAs) may prevent or arrest the pathogenesis of these diseases. Leaves of Sonchus oleraceus L. may be an excellent dietary LMWA source for humans given their apparent strong antioxidant activities in vitro. However, different S. oleraceus plants vary in their antioxidant capacity. Nothing is known of possible environmental effects on antioxidant potential. Equally, the effects of cooking and gastrointestinal digestion are unknown. The goals of this research were: (i) to study the effects of plant age, locality, and abiotic stressors on antioxidant potential; (ii) to study the effects of cooking and in vitro gastrointestinal digestion on antioxidant activity and uptake in human cells; and (iii) to study extractable antioxidant activities of S. oleraceus cell suspension cultures in relation to abiotic stressors.   Antioxidant activities and levels of total phenolics, hydroxycinnamic acids and ascorbate increased as plants aged. An ecotype from Acacia Bay had a higher phenolic content and antioxidant activities than one from Oamaru; these differences were maintained across generations as well as in calli from in vitro cultures. This indicates heritability and genetic fidelity of antioxidant potential.   Chilling and salinity had variable effects on concentrations of phenolics and antioxidant activities in plants, and the combination of the two stressors was not synergistic. This indicates that these two stressors share signalling and response pathways. Stressor-induced increases in antioxidant activities of leaf extracts correlated with improved cellular antioxidant activities (CAA) inside HepG2 cells. Antioxidants were released from leaves following in vitro gastrointestinal digestion, which were then subsequently uptaken by Caco2 and HepG2 cells wherein they displayed CAAs. Thus, elevated levels of antioxidants in stressor-imposed plants provide potentially more antioxidant protection to live human cells.  Caftaric, chlorogenic and chicoric acids accounted for 92% of the phenolic compounds in S. oleraceus leaves. Of these, only chlorogenic acid was inducible by stressors, both in intact plants and in calli. In young stressor-applied plants, chlorogenic acid was enhanced to the levels achievable with plant ageing.   Boiling leaves prior to digestion did not diminish the caftaric and chlorogenic acid levels released through digestion, but chicoric acid levels were. Out of the nine phenolic compounds in leaves, only chicoric, chlorogenic and caftaric acids were released into the medium during in vitro gastrointestinal digestion. Digestion of leaves resulted in effective release of caftaric and chlorogenic acids from leaves but the levels of released chicoric acid were diminished by digestion.  This study offers insights into the factors that influence the antioxidant potential of S. oleraceus L. in vivo, in vitro, during cooking and in vitro gastrointestinal digestion. These results provide the foundation for: (1) encouraging the consumption of its fresh shoots as an antioxidant rich food; (2) further improving its antioxidant activities through manipulation of agronomy, ecotype and breeding; (3) developing its cell cultures as a commercial platform for phyto-antioxidant production aimed at formulating dietary supplements or food additives in biopharmaceutical industry.</p>

scholarly journals Purification and Partial Characterization of Melanoidins Fractions from Toasted Oak Heartwood, Comparison with Melanoidins from Roasted Coffee

2018 ◽  
Vol 7 (6) ◽  
pp. 37 ◽  
Author(s):  
M. F. Nonier ◽  
N. Vivas ◽  
N. Vivas de Gaulejac ◽  
C. Mouche ◽  
C. Rossy Huguet ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Degradation Products ◽  
Partial Characterization ◽  
Maillard Reaction Products ◽  
Reaction Products ◽  
Roasted Coffee ◽  
Oak Wood ◽  
Processing And Storage ◽  
And Storage

During the cooking, processing, and storage of food products, a whole range of browning reactions occurs, initiated by the reaction of a carbohydrate with a compound possessing a free amino group. Melanoidins formed, influence food quality, mainly their colour, their flavour, and their antioxidant activities. Melanoidins are complex Maillard reaction products. We developed a method to isolate coffee melanoidins and melanoidins from toasted oak wood. We noted that coffee is richer in melanoidin compounds than oak wood. We presented a partial characterization of melanoidins fractions from toasted oak heartwood, and a comparison with melanoidins from roasted coffee. Mass spectra of the fractions isolated from toasted oak wood indicate the presence of pentose and hexose-based oligosaccharides with different degrees of polymerisation. The presence of the oligosaccharide moieties, as well as their degradation products found in the oak wood melanoidins, supports the postulated carbohydrate-based origin of melanoidins.

scholarly journals Domestic processing and storage on the physical-chemical characteristics of acerola juice (Malpighia glabra L.).

2019 ◽  
Vol 43 ◽  
Author(s):  
Bruno Martins Dala-Paula ◽  
Taís Pereira dos Santos ◽  
Laura de Souza Araújo ◽  
Renata Rosane Andrade Bastos ◽  
Juliana de Oliveira Moraes ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Titratable Acidity ◽  
Antioxidant Potential ◽  
Total Flavonoids ◽  
Tropical Fruit ◽  
Lack Of Information ◽  
Domestic Processing ◽  
Processing And Storage ◽  
And Storage ◽  
Seed Extracts

ABSTRACT Acerola is a tropical fruit that stands out for its high content of ascorbic acid and phenolic compounds. However, there is currently a lack of information on the effects of the preparation and domestic storage of fruit juices, especially that of acerola. The objective of this work was to evaluate the effect of different liquefying times (10, 30 and 50 s) and cold storage at 4 °C for 0, 24, 48 and 72 h in domestic acerola juice. In relation to such, physicochemical determinations were performed, including pH, total titratable acidity, antioxidant potential, contents of phenolic compounds and total flavonoids in juices produced from the whole fruit, the pulp and acerola seed extracts. Whole fruit juice, liquefied for 10 s, had the highest pH and lowest acidity averages (P≤0.05). The contents of phenolic and flavonoid compounds ranged from 26.06±1.18 to 168.34±24.63 mg of gallic acid equivalents (GAE)/100 mL and 11.17±0.96 to 49.45±1.43 mg of catechin equivalents (CE)/100 mL, respectively. Total phenolics were higher in whole acerola juices and total flavonoids in seed extracts, both when liquefied for 50 s. The antioxidant potential ranged from 0.12±0.01 to 4.26±0.78 mmol of Trolox equivalents (TE)/100 mL, and was also higher in whole acerola juice, liquefied for 50 s (P≤0.05). The results showed that acerola pulp has higher phenolic content and antioxidant potential, while seeds have more flavonoids. The liquefying time of the whole fruit for 50 s and consumption during the first 24 h, were the best conditions tested in this study, for obtaining a juice with a high content of phenolic and antioxidant activity.

scholarly journals Infusions of Herbal Blends as Promising Sources of Phenolic Compounds and Bioactive Properties

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2151 ◽  
Author(s):  
Tiane C. Finimundy ◽  
Carla Pereira ◽  
Maria Inês Dias ◽  
Cristina Caleja ◽  
Ricardo C. Calhelha ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Synergistic Effects ◽  
Sugar Content ◽  
Antimicrobial Activities ◽  
Phenolic Profile ◽  
Anti Inflammatory ◽  
The Individual ◽  
Bioactive Potential ◽  
Nutraceutical Products

Several plants have been used for medicinal applications and have been traditionally consumed as decoctions and infusions. Although some herbs are used alone as a beverage, they are often blended in mixtures to maximize their effects. Herein, the nutritional characterization of six infusions from herbal blends was evaluated using the official methods of analysis (AOAC international). A further characterization of the individual phenolic profile was also performed by HPLC-DAD/ESI-MSn, and finally bioactive potential was determined by evaluating the antioxidant, cytotoxic, anti-inflammatory, and antimicrobial activities of each blend. The wide variety of plants in each sample led to variability in the results for all analyzed parameters. However, blends containing 15% Laurus nobilis L. and 15% Juglan regia L. in their composition showed higher sugar content and energy contribution; higher concentration of phenolic compounds (phenolic acids and flavonoids); greater antioxidant, cytotoxic, and anti-inflammatory capacity; and also better antimicrobial effects against all the tested bacterial and fungal strains. Further studies will be necessary to evaluate the real synergistic effects that these two species show in the presence of other plants, and to evaluate their potential for application in various food, pharmaceutical, and nutraceutical products as infusion preparations.

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