scholarly journals Effects and Risk Assessment of the Polycyclic Musk Compounds Galaxolide® and Tonalide® on Marine Microalgae, Invertebrates, and Fish

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 371
Author(s):  
Friday Ojie Ehiguese ◽  
M. Judit González-Delgado ◽  
Carmen Garrido-Perez ◽  
Cristiano V. M. Araújo ◽  
M. Laura Martin-Diaz
Keyword(s):  
Environmental Risk ◽  
Sparus Aurata ◽  
Isochrysis Galbana ◽  
Aquatic Organisms ◽  
Paracentrotus Lividus ◽  
Marine Microalgae ◽  
Toxicity Tests ◽  
Marine Environments ◽  
Polycyclic Musk ◽  
Inhibition Concentration

The current research investigated the potential environmental risk of the polycyclic musk compounds, Galaxolide® (HHCB) and Tonalide® (AHTN), in the marine environments. These substances are lipophilic, bioaccumulated, and potentially biomagnified in aquatic organisms. To understand the toxicity of HHCB and AHTN, acute toxicity tests were performed by exposing marine microalgae (Phaeodactylum tricornutum, Tretraselmis chuii, and Isochrysis galbana), crustaceans (Artemia franciscana), echinoderms (Paracentrotus lividus), bivalves (Mytilus galloprovincialis), fish (Sparus aurata), and a candidate freshwater microalga (Raphidocelis subcapitata) to environmentally relevant concentrations (0.005–5 µg/L) following standardized protocols (US EPA, Environment Canada and OECD). P. tricornutum and I. galbana were sensitive to both substances and for P. tricornutum exposed to HHCB and AHTN, the IC10 values (the inhibition concentration at which 10% microalgae growth inhibition was observed) were 0.127 and 0.002 µg/L, respectively, while IC10 values calculated for I. galbana were 5.22 µg/L (a little higher than the highest concentration) and 0.328 µg/L, for HHCB and AHTN, respectively. Significant (p < 0.01) concentration dependent responses were measured in P. lividus and M. galloprovincialis larvae developments, as well as S. aurata mortality tested with HHCB. The effect of HHCB on P. lividus larvae development was the most sensitive endpoint recorded, producing an EC50 value (the effect concentration at which 50% effect was observed) of 4.063 µg/L. Considering the risk quotients both substances seem to represent high environmental risk to P. tricornutum and M. galloprovincialis in marine environments.

scholarly journals Effects and Risk Assessment of the Polycyclic Musk Compounds Galaxolide® and Tonalide® on Early Life Stages of Marine Organisms

2021 ◽  
Author(s):  
Friday Ojie Ehiguese ◽  
M. Judit González-Delgado ◽  
Carmen Garrido-Perez ◽  
Cristiano V.M. Araujo ◽  
Laura Martin-Diaz
Keyword(s):  
Early Life ◽  
Sparus Aurata ◽  
Isochrysis Galbana ◽  
Paracentrotus Lividus ◽  
Toxicity Tests ◽  
Life Stages ◽  
Marine Environments ◽  
Early Life Stages ◽  
Polycyclic Musk ◽  
Larvae Development

The current research investigated the environmental risk of the polycyclic musk compounds, Galaxolide&reg; (HHCB) and Tonalide&reg; (AHTN), in the marine environments. These substances are lipophilic, bioaccumulated and potentially biomagnified in aquatic organisms. To understand the toxicity of HHCB and AHTN, we performed acute toxicity tests by exposing marine microalgae (Phaeodactylum tricornutum, Tretraselmis chuii and Isochrysis galbana), crustaceans (Artemia franciscana), echinoderms (Paracentrotus lividus), bivalves (Mytilus galloprovincialis), fish (Sparus aurata) and a candidate freshwater microalga (Raphidocelis subcapitata) to environmentally relevant concentrations (0.005 - 5 &micro;g/L) following standardized protocols. The effects of both substances on microalgae growth were incipient and only I. galbana was sensitive to HHCB and AHTN, with IC10 values of 5.22 &micro;g/L and 0.328 &micro;g/L, respectively. Significant (p &lt; 0.01) concentration dependent responses were measured in P. lividus and M. galloprovincialis larvae developments as well as S. aurata mortality tested with HHCB. The effect of HHCB on P. lividus larvae development was the most sensitive endpoint recorded, producing an EC50 value of 4.07 &micro;g/L. Our results show that HHCB represents a high risk to P. lividus larvae development for early life stages in marine environments.

Acute and chronic effect of cyaniade on survival rate, behavior, and reproduction of Daphnia sp.

2013 ◽  
Vol 1 (1) ◽  
pp. 52 ◽  
Author(s):  
Melky R Pattiwael ◽  
Remy E. P Mangindaan ◽  
Rudi Prabowo ◽  
Inneke F. M Rumengan
Keyword(s):  
Government Regulation ◽  
Aquatic Organisms ◽  
Standard Test ◽  
Toxicity Tests ◽  
Zooplankton Organism ◽  
Rate Behavior ◽  
Negative Impacts ◽  
Reproduction Pattern ◽  
Acute And Chronic Effects ◽  
Ministerial Decree

Cyanide (CN) through leaching process in the gold processing could produce tailings that have negative impacts on the aquatic environment. To determine the acute and chronic effects of cyanide on aquatic organisms, toxicity tests have been conducted in the laboratory using Daphnia sp.This zooplankton organism is recommended by APHA as standard test animals. In this study the test concentrations refer to the Ministerial Decree No. 202 of 2004 and Government Regulation No. 82 of 2001. Cyanide was analyzed as free CN and WADS CN. The result of acute toxicity test using cyanide solution showed that Daphnia could survive a maximum concentration of 0.2 ppm. LC50 values for 24 and 72 hours were 0,59 mg/L and 0,10 mg/l, respectively. The LT50 was found at 42 hours. Daphnia sp. produced different numbers of offspring at different CN concentrations, and changed their reproduction pattern from parthenogenesis to sexual reproduction after exposure to a cyanide concentration of 0.1 ppm for 24 hours©  Sianida (CN) melalui proses leaching dalam proses pengolahan emas menghasilkan limbah yang dapat memberi dampak negatif bagi lingkungan perairan. Untuk mengetahui sampai seberapa jauh CN dapat memberi efek akut dan kronis terhadap biota air, telah dilakukan uji toksisitas di laboratorium dengan menggunakan Daphnia sp. Zooplankton ini direkomendasikan oleh APHA sebagai salah satu hewan uji standar. Dalam penelitian ini konsentrasi uji yang dipakai mengacu pada peraturan yang berlaku, yaitu Keputusan Menteri No. 202, Tahun 2004, dan Peraturan Pemerintah No. 82, Tahun 2001. Analisis sianida yang dihitung adalah nilai free CN dan WADS CN. Hasil penelitian uji toksisitas akut dengan menggunakan larutan sianida didapati Daphnia mampu bertahan hidup sampai pada konsentrasi 0,2 ppm dan nilai LC50 berada pada konsentarsi 0,1 ppm serta LT50 pada jam ke 42. Hasil uji efek kronis, Daphnia sp. menghasilkan jumlah anakan yang berbeda pada konsentrasi CN yang berlainan, dan mengalami perubahan pola reproduksi dari partenogenesis menjadi seksual setelah dipaparkan pada kosentrasi sianida 0,1 ppm selama 24 jam©

Metallic Elements (Cu, Zn, Ni and Mn) Toxicity Effects Determination on a Fresh Water Fish Cyprinus Carpio (Common Carp) Laboratory Acclimatized

2017 ◽  
Vol 68 (8) ◽  
pp. 1711-1715
Author(s):  
Stefania Gheorghe ◽  
Gabriela Geanina Vasile ◽  
Cristina Gligor ◽  
Irina Eugenia Lucaciu ◽  
Mihai Nita Lazar
Keyword(s):  
Cyprinus Carpio ◽  
Aquatic Organisms ◽  
Control Fish ◽  
Toxicity Tests ◽  
Fresh Water Fish ◽  
Metallic Elements ◽  
Mn Toxicity ◽  
Vitro Effect

Metallic elements copper (Cu), zinc (Zn), nickel (Ni) and manganese (Mn) are some of the most commonly found in water and sediment samples collected from the Danube - Danube Delta. These elements are important as essential micronutrients, being normally present at low concentrations in biological organisms, but in high concentrations they become toxic with immediate and delayed effects. The role of this metals is still controversial, that�s why bioconcentration potential is so important. In this non-clinical study, we tested in vitro effect of heavy metals on carp, Cyprinus carpio, reproducing in vivo presence of Cu, Zn, Ni and Mn in the Romanian�s surface water. The toxicity tests were performed according to OECD 203 by detecting the average (50%) lethal concentration - LC50 on aquatic organisms (freshwater fish) at 96h. The results pointed out that, copper value for LC 50 at 96h was estimated as 3.4 mg/L (concentrations tested in the range of 0.1 - 4.75 mg/L). Zinc value for LC 50 at 96h was estimated as 20.8 mg/L (concentrations tested in the range of 0.028 � 29.6 mg/L). Nickel value for LC 50 at 96h was estimated as 40.1 mg/L (concentrations tested in the range of 0.008 - 84.5 mg/L). For manganese the mortality effects has recorded at LC 50 at 96h at estimated value higher than 53 mg/L (concentrations tested in the range of 0.04 - 53.9 mg/L). The accuracy of the testing metals concentration was insured by the screening of the dilution water, as well as food and control fish, acclimated in laboratory conditions.

scholarly journals Environmental Risk Assessment Resulting from Sediment Contamination with Perfluoroalkyl Substances

Molecules ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 116
Author(s):  
Grażyna Gałęzowska ◽  
Justyna Rogowska ◽  
Ewa Olkowska ◽  
Wojciech Ratajczyk ◽  
Lidia Wolska
Keyword(s):  
Risk Assessment ◽  
Environmental Risk ◽  
Environmental Risk Assessment ◽  
Treatment Plant ◽  
River Mouth ◽  
Ultra Performance Liquid Chromatography ◽  
Perfluoroalkyl Substances ◽  
Toxicity Tests ◽  
Aquatic Life ◽  
Vistula River

Due to wide use of perfluoroalkyl substances (PFASs) (e.g., in metal-plating, in fire-fighting foam, lubricants) and their resistance to degradation, they occur widely in the environment. The aim of this study was to estimate the environmental risk resulting from the presence of PFASs in the Gulf of Gdansk. Therefore, 17 PFASs concentrations were determined using ultra performance liquid chromatography with tandem mass spectrometry detection (UPLC-MS/MS). Additionally, sediment ecotoxicity was investigated. The results of the chemical analysis were used to asses environmental risk of PFASs. In samples collected around discharge collectors from a wastewater treatment plant and the Vistula mouth, Σ17PFASs values were 0.00403 ÷ 40.6 and 0.509 ÷ 614 ng/g d.w., respectively. In samples collected around discharge collectors, PFHxA, PFPeA, PFHpA, and PFOA were dominating, while at the Vistula River mouth, PFHxS, PFDS, and PFBS were prevalent. For most sediments, no toxic effect was observed in the toxicity tests with Heterocypris inconguens and Aliivibrio ficsheri. There was no observed correlation between the PFASs level and their ecotoxicity. Generally, the results of environmental risk assessment indicate that the PFASs would not generate high impact on the aquatic life (five water samples have shown medium risk related to PFBS and PFDoA).

scholarly journals Risk Assessment of Malakite with the Active Substances Dithianon and Pyrimenthanil

2019 ◽  
pp. 312-314
Author(s):  
Torsten Källqvist ◽  
Merete Grung ◽  
Katrine Borgå ◽  
Hubert Dirven ◽  
Ole Martin Eklo ◽  
...  
Keyword(s):  
Risk Assessment ◽  
Surface Waters ◽  
Plant Protection ◽  
Aquatic Organisms ◽  
Toxicity Tests ◽  
Concentration Addition ◽  
Active Ingredients ◽  
Active Substances ◽  
Assessment Factor

The plant protection product Malakite (BAS 669 01 F), containing the active substances dithianon and pyrimethanil, is a fungicide against scab in pome fruits. Products containing these active plant protection substances are approved in Norway, but not with both substances in the same product. The Swedish Chemicals Agency (KemI) has as zonal Rapporteur Member State (zRMS) of the Northern Zone evaluated the product Malakite and decided on non-approval due to the observation of unacceptable effects in exposed birds, aquatic organisms, non-target arthropods and earthworms. On request from The Norwegian Food Safety Authority, the VKM Panel on Plant Protection Products has discussed the available data and the report prepared by KemI, and has concluded as follows on the questions raised: On the refinement of DT50 in long term risk assessment for birds: It is the view of the VKM panel that the refinement is not acceptable because the analysis using first order kinetics seems not in line with a realistic and sufficiently conservative approach for the data provided. Furthermore, field studies from more sites are required. On the long term cumulative effects of the active substances on birds: VKM shares the view of KemI, that the combined sub-lethal and reproduction effects should be assessed because the mode of action of the two ingredients has only been shown in fungi, and since the mechanisms in birds could be different. On the reduction of assessment factor for fish: VKM opposes to the reduction of assessment factor for dithianon in fish because the data from acute toxicity tests cannot be extrapolated to chronic toxicity, and because the factor should reflect not only the variation in interspecies sensitivity, but also the uncertainty involved in extrapolation from laboratory tests to the field situation. On the choice of end point in risk assessment for fish: The VKM panel considers the NOEC of dithianon for fish determined from the study at pH 7.9 not to be adequate for the more acidic Norwegian surface waters, and recommends using the data from the test performed at pH 6.5. On the formulation studies for aquatic organisms: It is the opinion of the VKM panel that the formulation studies may be used together with corresponding studies with the active ingredients as long as the studies compared are performed and evaluated according to the same principles. However, VKM notes that the formulation tests as well as the tests of the active ingredients have been performed at high pH values, which are not representative to most Norwegian surface waters. Thus, the toxic effect of dithianon shown in these tests are likely to be lower than expected under typical conditions in Norway. On the assessment factors for concentration addition in fish: It is the opinion of the VKM panel that a reduction in assessment factor for one component in a mixture cannot be used for a formulation containing components for which a similar reduction has not been accepted. On effect studies of active substances and formulations on non-target arthropods: The VKM panel shares the view of KemI that the risk assessment should be based on all available information, including the studies presented for the active substances. On the endpoint in earthworm risk assessment: VKM supports the view of KemI that the observed effects of pyrimethanil on reproduction of earthworms should be considered in the risk assessment of Malakite.

scholarly journals Taking Full Advantage of Modelling to Better Assess Environmental Risk Due to Xenobiotics

2021 ◽  
Author(s):  
Sandrine Charles ◽  
Aude Ratier ◽  
Virgile Baudrot ◽  
Gauthier Multari ◽  
Aurélie Siberchicot ◽  
...  
Keyword(s):  
Environmental Risk ◽  
Aquatic Organisms ◽  
The European Union ◽  
Time Dimension ◽  
New Era ◽  
Individual Level ◽  
Regulatory Bodies ◽  
On Line ◽  
The Individual ◽  
User Friendly

Abstract In the European Union, more than 100,000 man-made chemical substances are awaiting an environmental risk assessment (ERA). Simultaneously, ERA of chemicals has now entered a new era. Indeed, recent recommendations from regulatory bodies underline a crucial need for the use of mechanistic effect models, allowing assessments that are not only ecologically relevant, but also more integrative, consistent and efficient. At the individual level, toxicokinetic-toxicodynamic (TKTD) models are particularly encouraged for the regulatory assessment of pesticide-related risks on aquatic organisms. In this paper, we first propose a brief review of classical dose-response models to put into light the on-line MOSAIC tool offering all necessary services in a turnkey web platform whatever the type of data to analyze. Then, we focus on the necessity to account for the time-dimension of the exposure by illustrating how MOSAIC can support a robust calculation of bioaccumulation factors. At last, we show how MOSAIC can be of valuable help to fully complete the EFSA workflow regarding the use of TKTD models, especially with GUTS models, providing a user-friendly interface for calibrating, validating and predicting survival over time under any time-variable exposure scenario of interest. Our conclusion proposes a few lines of thought for an even easier use of modelling in ERA.

Effect of TiO 2 nanoparticle aggregation on marine microalgae Isochrysis galbana

2018 ◽  
Vol 66 ◽  
pp. 208-215 ◽  
Author(s):  
Ji Hu ◽  
Jianmin Wang ◽  
Shuxia Liu ◽  
Zhechao Zhang ◽  
Haifeng Zhang ◽  
...  
Keyword(s):  
Isochrysis Galbana ◽  
Marine Microalgae ◽  
Nanoparticle Aggregation

Influence of calcium and EDTA on copper ion bioavailability in copper nanoparticle toxicity tests improves understanding of nano-specific effects

2020 ◽  
Vol 36 (7) ◽  
pp. 467-476
Author(s):  
Halis Boran
Keyword(s):  
Acute Toxicity ◽  
Metal Ions ◽  
Ethylenediaminetetraacetic Acid ◽  
Scale Effects ◽  
Copper Ion ◽  
Chelating Agent ◽  
Aquatic Organisms ◽  
Toxicity Tests ◽  
Cu Toxicity ◽  
Cu Ions

Metal-based nanoparticles (NPs) can release metal ions that are toxic to aquatic organisms; however, whether the toxicity is from metal ions rather than unique “nano-scale” effects of the NPs is unresolved. The present study aimed to compare the toxicity of Cu2+ and Cu-NPs in larval zebrafish ( Danio rerio) to clarify whether toxic effects are attributable to release of Cu ions and to determine the effect of the chelating agent ethylenediaminetetraacetic acid (EDTA) and calcium hardness (as CaCO3) on the Cu toxicity. First, the acute toxicity (96-h lethality) of Cu-NPs was determined in comparison to aqueous Cu in larvae exposed to CuSO4, and subsequently, sublethal tests with Cu-NPs and CuSO4 were conducted with additions of EDTA or calcium ions to evaluate alterations in expression of metallothionein-2 ( MT2) gene transcripts (quantitative real-time polymerase chain reaction). Acute toxicity of Cu in larvae exposed to CuSO4 was greater (LC50 = 226 µg Cu/L) than for larvae exposed to Cu-NPs (LC50 = 648 µg Cu/L). The expression of MT2 increased with Cu concentration ( p < 0.05), and the slope of the linear regression was significantly greater in fish exposed to CuSO4 (slope = 0.090) compared to Cu-NPs (slope = 0.011). Cu2+ was 2.9-fold more toxic than Cu-NPs. The presence of 5 mg/L EDTA and 220 mg/L CaCO3 significantly reduced the expression of MT2 (1.8-fold for EDTA, 2.3-fold for CaCO3) in larvae exposed to CuSO4. For larvae exposed to Cu-NPs, the presence of EDTA reduced the expression of MT2 (1.7-fold) relative to Cu-NP concentration. While Cu-NPs induced MT2 expression, the differences in concentration relationships of MT2 expression between Cu-NPs and CuSO4 indicated that factors other than release of Cu ions from Cu-NPs influenced acute toxicity of Cu-NPs. The conclusion drawn from this ecotoxicological risk assessment was that EDTA and calcium significantly decreased Cu toxicity in freshwater fish.

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