scholarly journals Biofilm Degradation of Nontuberculous Mycobacteria Formed on Stainless Steel Following Treatment with Immortelle (Helichrysum italicum) and Common Juniper (Juniperus communis) Essential Oils

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 362
Author(s):  
Dolores Peruč ◽  
Dalibor Broznić ◽  
Željka Maglica ◽  
Zvonimir Marijanović ◽  
Ljerka Karleuša ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Essential Oils ◽  
Laser Scanning ◽  
Nontuberculous Mycobacteria ◽  
Bacterial Count ◽  
Water Disinfection ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Juniperus Communis ◽  
Common Juniper

Nontuberculous mycobacteria, like other opportunistic premise plumbing pathogens, produce resistant biofilms on various surfaces in the plumbing system including pipes, tanks, and fittings. Since standard methods of water disinfection are ineffective in eradicating biofilms, research into new agents is necessary. Essential oils (EOs) have great potential as anti-biofilm agents. Therefore, the purpose of this research was to investigate the potential anti-biofilm effect of common juniper (Juniperus communis) and immortelle (Helichrysum italicum) EOs. Minimum inhibitory concentrations (MIC), minimum bactericidal concentrations (MBC), and minimum effective concentrations of EOs on Mycobacterium avium, M. intracellulare, and M. gordonae were tested. Additionally, biofilms on the surface of a stainless steel disc were treated with single or mixed concentration of EOs, in order to investigate their degeneration via the bacterial count and confocal laser scanning microscopy (CLSM). H. italicum EO showed the strongest biofilm degradation ability against all Mycobacteria strains that were tested. The strongest effect in the biofilm degradation after the single or mixed applications of EOs was observed against M. gordonae, followed by M. avium. The most resistant was the M. intracellulare biofilm. Synergistic combinations of J. communis and H. italicum EOs therefore seem to be an effective substance in biofilm degradation for use in small water systems such as baths or hot tubs.

scholarly journals Inhibitive Properties of Benzyldimethyldodecylammonium Chloride on Microbial Corrosion of 304 Stainless Steel in a Desulfovibrio desulfuricans-Inoculated Medium

Materials ◽  
2019 ◽  
Vol 12 (2) ◽  
pp. 307 ◽  
Author(s):  
Chung-Wen Hsu ◽  
Tzu-En Chen ◽  
Kai-Yin Lo ◽  
Yueh-Lien Lee
Keyword(s):  
Stainless Steel ◽  
Laser Scanning ◽  
Steel Substrate ◽  
Desulfovibrio Desulfuricans ◽  
Corrosion Current ◽  
Laser Scanning Microscopy ◽  
304 Stainless Steel ◽  
Confocal Laser ◽  
Microbial Corrosion ◽  
Sulfate Reducing

Biocides are frequently used to control sulfate-reducing bacteria (SRB) in biofouling. The increasing restrictions of environmental regulations and growing safety concerns on the use of biocides result in efforts to minimize the amount of biocide use and develop environmentally friendly biocides. In this study, the antimicrobial activity and corrosion inhibition effect of a low-toxic alternative biocide, benzyldimethyldodecylammonium chloride (BDMDAC), on a 304 stainless steel substrate immersed in a Desulfovibrio desulfuricans (D. desulfuricans)-inoculated medium was examined. Potentiodynamic polarization curves were used to analyze corrosion behavior. Biofilm formation and corrosion products on the surfaces of 304 stainless steel coupons were examined using scanning electron microscopy (SEM), energy-dispersive X-ray spectrum, and confocal laser scanning microscopy (CLSM). Results demonstrated that this compound exhibited satisfactory results against microbial corrosion by D. desulfuricans. The corrosion current density and current densities in the anodic region were lower in the presence of BDMDAC in the D. desulfuricans-inoculated medium. SEM and CLSM analyses revealed that the presence of BDMDAC mitigated formation of biofilm by D. desulfuricans.

scholarly journals Effect of Clove and Thyme Essential Oils on Candida Biofilm Formation and the Oil Distribution in Yeast Cells

Molecules ◽  
2019 ◽  
Vol 24 (10) ◽  
pp. 1954 ◽  
Author(s):  
Katarzyna Rajkowska ◽  
Paulina Nowicka-Krawczyk ◽  
Alina Kunicka-Styczyńska
Keyword(s):  
Essential Oils ◽  
Biofilm Formation ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Yeast Cells ◽  
Confocal Laser ◽  
Minimal Inhibitory Concentrations ◽  
Candida Sp ◽  
A Cell ◽  
Candida Biofilm

Candida biofilm structure is particularly difficult to eradicate, since biofilm is much more resistant to antifungal agents than planktonic cells. In this context, a more effective strategy seems to be the prevention of biofilm formation than its eradication. The aim of the study was to examine whether the process of initial colonization of materials (glass, polyethylene terephthalate, polypropylene) by food-borne Candida sp. can be impeded by clove and thyme essential oils, used at their minimal inhibitory concentrations. In the presence of clove oil, 68.4–84.2% of the yeast tested showed a statistically significant reduction in biofilm formation, depending on the material. After treatment with thyme oil, statistically significant decrease in biofilm cell numbers was observed for 63.2–73.7% of yeasts. Confocal laser scanning microscopy showed diverse compounds of clove and thyme oils that were disparately located in C. albicans cell, on a cell wall and a cell membrane, in cytoplasm, and in vacuoles, depicting the multidirectional action of essential oils. However, essential oils that were used in sub-inhibitory concentration were sequestrated in the yeast vacuoles, which indicate the activation of Candida defense mechanisms by cell detoxification. Clove and thyme essential oils due to their anti-biofilm activity can be efficiently used in the prevention of the tested abiotic surfaces colonization by Candida sp.

Culture and Detection of Campylobacter jejuni within Mixed Microbial Populations of Biofilms on Stainless Steel

2007 ◽  
Vol 70 (6) ◽  
pp. 1379-1385 ◽  
Author(s):  
SHERIASE Q. SANDERS ◽  
DOROTHY H. BOOTHE ◽  
JOSEPH F. FRANK ◽  
JUDY W. ARNOLD
Keyword(s):  
Stainless Steel ◽  
Campylobacter Jejuni ◽  
Laser Scanning ◽  
The United States ◽  
Laser Scanning Microscopy ◽  
Epifluorescence Microscopy ◽  
Confocal Laser ◽  
Atmospheric Conditions ◽  
Culture Methods ◽  
Bacterial Populations

Campylobacter jejuni is the most frequently reported cause of foodborne illness in the United States, but its survival outside the host is poor. The objective of this research was to examine the formation and composition of biofilms by C. jejuni alone and within mixed bacterial populations from the poultry-processing environment. C. jejuni growth was assessed with four media, two temperatures, and two atmospheric conditions to develop culture methods for liquid media that would allow growth within the biofilms. Growth kinetics was followed at four cell densities to determine temporal compatibility within biofilm mixtures. Analysis of the biofilms by confocal laser scanning microscopy showed that C. jejuni formed a biofilm when incubated without other bacteria. The average surface area of stainless steel covered by C. jejuni increased by 50% from 24 to 48 h, remained level to 96 h, and then decreased by 88% by 168 h. C. jejuni and mixed bacterial populations formed biofilms during incubation periods of up to 7 days. The area of the mixture was significantly greater than for C. jejuni alone at 24 h, was approximately the same at 48 h, and was significantly less by 168 h. When incubated with either of two initial inoculum densities of other bacteria, the number of C. jejuni was enhanced after 24 h. The intensity of fluorescence and cell viability were monitored by epifluorescence microscopy. This study provides the basis for studying interactions of Campylobacter spp. with other bacteria in the environment, which will aid in the design of effective intervention strategies.

scholarly journals Antimicrobial and Anticorrosion Activity of a Novel Composite Biocide against Mixed Bacterial Strains in Taiwanese Marine Environments

Materials ◽  
2021 ◽  
Vol 14 (20) ◽  
pp. 6156
Author(s):  
Soul-Yi Chang ◽  
Shih-Yen Huang ◽  
Yu-Ren Chu ◽  
Shun-Yi Jian ◽  
Kai-Yin Lo ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Laser Scanning ◽  
Electrochemical Impedance ◽  
Laser Scanning Microscopy ◽  
304 Stainless Steel ◽  
Single Type ◽  
Confocal Laser ◽  
Subtropical Climate ◽  
Marine Environments ◽  
Bacterial Strains

Taiwan is an island with a humid subtropical climate. The relatively warm seawater results in biofouling of the surfaces of marine facilities. Biocide application is a common practice for combating and eliminating adhesive fouling. However, a single type of biocide may have limited antimicrobial effects due to the relatively high microbial diversity in marine environments. Therefore, applying a mixture of various biocides may be necessary. In this study, the antimicrobial and anticorrosion properties of a newly designed composite biocide, namely a combination of thymol and benzyldimethyldodecylammonium chloride, were investigated by applying the biocide to 304 stainless steel substrates immersed in inocula containing bacterial strains from Tamsui and Zuoying harbors. The ability of 3TB and 5TB treatments to prevent sessile cells and biofilm formation on the 304 stainless steel coupon surface was determined through scanning electron microscopy investigation. In addition, confocal laser scanning microscopy indicated that the 5TB treatment achieved a greater bactericidal effect in both the Tamsui and Zuoying inocula. Moreover, electrochemical impedance spectroscopy revealed that the diameter of the Nyquist semicircle was almost completely unaffected by Tamsui or Zuoying under the 5TB treatment. Through these assessments of antimicrobial activity and corrosion resistance, 5TB treatment was demonstrated to have superior bactericidal activity against mixed strains in both southern and northern Taiwanese marine environments.

scholarly journals Enhanced Inactivation of Salmonella and Pseudomonas Biofilms on Stainless Steel by Use of T-128, a Fresh-Produce Washing Aid, in Chlorinated Wash Solutions

2012 ◽  
Vol 78 (19) ◽  
pp. 6789-6798 ◽  
Author(s):  
Cangliang Shen ◽  
Yaguang Luo ◽  
Xiangwu Nou ◽  
Gary Bauchan ◽  
Bin Zhou ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Organic Matter ◽  
Laser Scanning ◽  
Hypochlorous Acid ◽  
Free Chlorine ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Content Type ◽  
Cell Responses ◽  
Depletion Rate

ABSTRACTThe effect of the washing aid T-128 (generally recognized as safe [GRAS] formulation, composed mainly of phosphoric acid and propylene glycol) on inactivation ofSalmonellaandPseudomonaspopulations in biofilms on stainless steel was evaluated under conditions of increasing organic matter loads in chlorinated wash solutions dominated by hypochlorous acid. Biofilms were formed statically on stainless steel coupons suspended in 2% lettuce extract after inoculation withSalmonella entericaserovar Thompson or Newport or withPseudomonas fluorescens. Coupons with biofilms were washed in chlorine solutions (0, 0.5, 1, 2, 5, 10, or 20 mg/liter at pH 6.5, 5.0 and 2.9), with or without T-128, and with increasing loads of organic matter (0, 0.25, 0.5, 0.75, or 1.0% lettuce extract). Cell populations on coupons were dispersed using intermittent, pulsed ultrasonication and vortexing and enumerated by colony counts on XLT-4 orPseudomonasagars. Cell responses to fluorescent viability staining of biofilm treatment washing solutions were examined using confocal laser scanning microscopy. Results showed that 0.1% T-128 (without chlorine) reducedP. fluorescensbiofilm populations by 2.5 log10units but did not reduceSalmonellapopulations. For bothSalmonellaandPseudomonas, the sanitizing effect of free chlorine (1.0 to 5.0 mg/liter) was enhanced (P< 0.05) when it was combined with T-128. Application of T-128 decreased the free chlorine depletion rate caused by increasing organic matter in wash waters and significantly (P< 0.05) augmented inactivation of bacteria in biofilms compared to treatments without T-128. Image analysis of surfaces stained with SYTO and propidium iodide corroborate the cultural assay results showing that T-128 can aid in reducing pathogen viability in biofilms and thus can aid in sanitizing stainless steel contact surfaces during processing of fresh-cut produce.

scholarly journals Inactivation Efficacy of 405 nm LED Against Cronobacter sakazakii Biofilm

2020 ◽  
Vol 11 ◽  
Author(s):  
Yixiao Huang ◽  
Quanwei Pei ◽  
Ruisha Deng ◽  
Xiaoying Zheng ◽  
Jialu Guo ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Laser Scanning ◽  
Light Emitting Diode ◽  
Treated Group ◽  
Attenuated Total Reflection ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Pcr Analysis ◽  
Cronobacter Sakazakii ◽  
Sensitivity Change

The objectives of this study were to evaluate the inactivation efficacy of a 405-nm light-emitting diode (LED) against Cronobacter sakazakii biofilm formed on stainless steel and to determine the sensitivity change of illuminated biofilm to food industrial disinfectants. The results showed that LED illumination significantly reduced the population of viable biofilm cells, showing reduction of 2.0 log (25°C), 2.5 log (10°C), and 2.0 log (4°C) between the non-illuminated and LED-illuminated groups at 4 h. Images of confocal laser scanning microscopy and scanning electron microscopy revealed the architectural damage to the biofilm caused by LED illumination, which involved destruction of the stereoscopic conformation of the biofilm. Moreover, the loss of biofilm components (mainly polysaccharide and protein) was revealed by attenuated total reflection Fourier-transformed infrared spectroscopy, and the downregulation of genes involved in C. sakazakii biofilm formation was confirmed by real time quantitative PCR analysis, with greatest difference observed in fliD. In addition, the sensitivity of illuminated-biofilm cells to disinfectant treatment was found to significantly increased, showing the greatest sensitivity change with 1.5 log reduction between non-LED and LED treatment biofilms in the CHX-treated group. These results indicated that 405 nm LED illumination was effective at inactivating C. sakazakii biofilm adhering to stainless steel. Therefore, the present study suggests the potential of 405 nm LED technology in controlling C. sakazakii biofilms in food processing and storage, minimizing the risk of contamination.

Biofilm Formation and Stainless Steel Corrosion Analysis of Leptothrix discophora

2015 ◽  
Vol 1130 ◽  
pp. 79-82 ◽  
Author(s):  
Christian Thyssen ◽  
David Holuscha ◽  
Jens Kuhn ◽  
Friederike Walter ◽  
Wolfram Fürbeth ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Biofilm Formation ◽  
Manganese Oxides ◽  
Laser Scanning ◽  
Steel Corrosion ◽  
Open Circuit ◽  
Laser Scanning Microscopy ◽  
Microbiologically Influenced Corrosion ◽  
Confocal Laser ◽  
Pitting Potential

Bioleaching and biocorrosion are based on similar biochemical processes. Microbe-surface interaction, biofilm formation and concomitant extracellular polymeric substance (EPS) production gained increasing interest in the past decades. Nowadays it is generally accepted that biofilm formation and an accompanying formation of manganese oxides by manganese oxidizing bacteria such as Leptothrix spp. account for one type of pitting corrosion of stainless steel (SS). However, little is known about biofilm formation, EPS composition of manganese oxidizing microorganisms and their influence on microbiologically influenced corrosion. Consequently, we studied biofilm formation of Leptothrixdiscophora, the biooxidation of manganese in biofilms on floating filters as well as biofilm formation on stainless steel and the involved corrosion processes. Cells were visualized by epifluorescence (EFM) or confocal laser scanning –microscopy (CLSM). Additionally, the influence of biofilm formation and biooxidation of manganese by L. discophora on the open circuit potential (OCP) and pitting potential (Epit) of stainless steel was measured using a 3 electrode setup. L. discophora grew well in biofilms on floating filters and on SS coupons and incorporated in both conditions Mn2+ in the form of MnO2 from the bulk phase into the biofilm. OCP measurements of actively manganese-oxidizing biofilms on stainless steel showed a significant ennoblement of ≥200 mV.

scholarly journals Development of a methodology for measuring the evolution of duplex stainless-steel low-temperature plasma nitrided phases expansion using confocal laser scanning microscopy

2021 ◽  
Vol 40 (1) ◽  
Author(s):  
Carlos Eduardo Alves Feitosa ◽  
Rodrigo Perito Cardoso ◽  
Silvio Francisco Brunatto
Keyword(s):  
Stainless Steel ◽  
Low Temperature ◽  
Duplex Stainless Steel ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Low Temperature Plasma ◽  
Temperature Plasma ◽  
Innovative Methodology ◽  
Different Response

Samples of duplex stainless steel SAF 2507 were low-temperature plasma nitrided to characterize separately, on the surface, the behavior of its ferrite and austenite phases in relation to two competing processes, that is, one caused by enrichment by nitrogen, resulting in possible expansion, and the other caused by the removal of superficial atoms via sputtering, which may lead to the retraction of the studied phases. Since these phases have different different compositions and crystalline structures, of which the diffusivity and solubility of nitrogen in them are dependent, a different response for each type of phase can be expected. In this article, an innovative methodology has been developed to quantify and clarify which effects are predominant in the course of nitriding for each of these phases. The results indicate that phase expansion prevails over sputtering.

scholarly journals Electric Current-Induced Detachment of Staphylococcus epidermidis Biofilms from Surgical Stainless Steel

2004 ◽  
Vol 70 (11) ◽  
pp. 6871-6874 ◽  
Author(s):  
Arnout J. van der Borden ◽  
Hester van der Werf ◽  
Henny C. van der Mei ◽  
Henk J. Busscher
Keyword(s):  
Stainless Steel ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Electric Currents ◽  
Experimental Conditions ◽  
Current Application ◽  
Percutaneous Implants

ABSTRACT Biomaterial-centered infections of orthopedic percutaneous implants are serious complications which can ultimately lead to osteomyelitis, with devastating effects on bone and surrounding tissues, especially since the biofilm mode of growth offers protection against antibiotics and since removal frequently is the only ultimate solution. Recently, it was demonstrated that as a possible pathway to prevent infections of percutaneous stainless steel implants, electric currents of 60 to 100 μA were effective at stimulating the detachment of initially adhering staphylococci from surgical stainless steel. However, initially adhering bacteria are known to adhere more reversibly than bacteria growing in the later stages of biofilm formation. Hence, the aim of this study was to examine whether a growing Staphylococcus epidermidis biofilm can be stimulated to detach from surgical stainless steel by the use of electric currents. In separate experiments, four currents, i.e., 60 and 100 μA of direct current (DC) and 60 and 100 μA of block current (50% duty cycle, 1 Hz), were applied for 360 min to stimulate the detachment of an S. epidermidis biofilm that had grown for 200 min. A 100-μA DC yielded 78% detachment, whereas a 100-μA block current under the same experimental conditions yielded only 31% detachment. The same trend was found for 60 μA, with 37% detachment for a DC and 24% for a block current. Bacteria remaining on the surface after the current application were less viable than they were prior to the current application, as demonstrated by confocal laser scanning microscopy. In conclusion, these results suggest that DCs are preferred for curing infections.

3-D imaging of cells using a confocal laser scanning microscope and digital image processing

1988 ◽  
Vol 46 ◽  
pp. 96-97
Author(s):  
Thomas M. Jovin ◽  
Michel Robert-Nicoud ◽  
Donna J. Arndt-Jovin ◽  
Thorsten Schormann
Keyword(s):  
Laser Scanning ◽  
Confocal Laser Scanning Microscope ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Scanning Microscope ◽  
Laser Scanning Microscope ◽  
Biochemical Processes ◽  
Adjacent Structures

Light microscopic techniques for visualizing biomolecules and biochemical processes in situ have become indispensable in studies concerning the structural organization of supramolecular assemblies in cells and of processes during the cell cycle, transformation, differentiation, and development. Confocal laser scanning microscopy offers a number of advantages for the in situ localization and quantitation of fluorescence labeled targets and probes: (i) rejection of interfering signals emanating from out-of-focus and adjacent structures, allowing the “optical sectioning” of the specimen and 3-D reconstruction without time consuming deconvolution; (ii) increased spatial resolution; (iii) electronic control of contrast and magnification; (iv) simultanous imaging of the specimen by optical phenomena based on incident, scattered, emitted, and transmitted light; and (v) simultanous use of different fluorescent probes and types of detectors.We currently use a confocal laser scanning microscope CLSM (Zeiss, Oberkochen) equipped with 3-laser excitation (u.v - visible) and confocal optics in the fluorescence mode, as well as a computer-controlled X-Y-Z scanning stage with 0.1 μ resolution.

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