scholarly journals A Fructan Sucrase Secreted Extracellular and Purified in One-Step by Gram-Positive Enhancer Matrix Particles

Processes ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 95
Author(s):  
Jingyue Wang ◽  
Huazhi Xiao ◽  
Fangkun Zhao ◽  
Bo Zhao ◽  
Min Xu ◽  
...  
Keyword(s):  
Large Scale ◽  
Recombinant Enzyme ◽  
Recombinant Enzymes ◽  
Step Method ◽  
Enzymatic Production ◽  
Extracellular Secretion ◽  
Purification System ◽  
Induction Experiment ◽  
One Step ◽  
Selection For

Fructan sucrase is a kind of biological enzyme that catalyzes the synthesis of fructan, and fructan is a polysaccharide product with important industrial application value. In this study, the Fructan sucrase gene of Bacillus subtilis was cloned to plasmid PET-28A-ACMA-Z, and three clones were obtained after the transformation of Escherichia coli BL21, namely BS-FF, BSO, and BS. The clones BS-FF and BSO secreted the recombinant enzymes outside the cells, while the clone BS expressed them inside the cells. The induction experiment results showed that the optimum IPTG concentration in the medium was 0.5 mM and 1.0 mM for clones BS-FF and BSO, respectively, while the incubation conditions were at 28 °C for 8 h. The recombinant fructan sucrase was purified one step using a material called GEM particles. The results indicated that 95.25% of fructan sucrase expressed by the clone BS-FF could be secreted into the extracellular area, and even 98.78% by the clone BSO. With the above purification system, the receiving rate of the recombinant enzyme for clones BS-FF and BSO was 97.70% and 84.99%, respectively. As for the bioactivity of recombinant fructan sucrase, the optimum temperature and pH were 50 °C and 5.6, respectively. The Km and Vmax of it were 33.96 g/L and 0.63 g/(L·min), respectively. The engineered strains with the high extracellular secretion of fructan sucrase were constructed, and a one-step method for the purification of the recombinant enzyme was established. The results might provide a novel selection for the enzymatic production of fructan on a large scale.

scholarly journals Aqueous dispersion and tuning surface charges of polytetrafluoroethylene particles by bioinspired polydopamine–polyethyleneimine coating via one-step method

2021 ◽  
Vol 8 (8) ◽  
pp. 210582
Author(s):  
Manjit Singh Grewal ◽  
Hiroya Abe ◽  
Yasutaka Matsuo ◽  
Hiroshi Yabu
Keyword(s):  
Surface Modification ◽  
Large Scale ◽  
Surface Coverage ◽  
Aqueous Dispersion ◽  
Surface Charges ◽  
Step Method ◽  
Reaction Solution ◽  
Uniform Dispersion ◽  
One Step ◽  
Inert Surfaces

We propose a surface modification of poorly dispersive polytetrafluoroethylene (PTFE) particles via bioinspired polydopamine–polyethyleneimine (PDA–PEI) which conferred PTFE particles a uniform dispersion in aqueous medium. With increasing dopamine concentration in the reaction solution, dispersity of PTFE particles improved and the surface charges of particles changed from negative to positive due to an increase of surface coverage of PDA–PEI layers. Simplicity of the method here outlines an attractive route for surface modification of inert surfaces useful for large-scale applications.

Hollow Co3O4 microspheres with nano-sized shells: one-step large-scale synthesis, growth mechanism and supercapacitor properties

RSC Advances ◽  
2015 ◽  
Vol 5 (52) ◽  
pp. 42055-42062 ◽  
Author(s):  
Chao Feng ◽  
Jinfeng Zhang ◽  
Yida Deng ◽  
Cheng Zhong ◽  
Lei Liu ◽  
...  
Keyword(s):  
Growth Mechanism ◽  
Large Scale ◽  
Organic Reagent ◽  
Step Method ◽  
Spherical Shells ◽  
Hydrothermal Temperature ◽  
One Step ◽  
Subsequent Calcination ◽  
Large Scale Synthesis ◽  
Co3o4 Microspheres

Hollow Co3O4 microspheres with thin spherical shells were produced using a one-step method at a low hydrothermal temperature (120 °C), and could be obtained without the use of a solid template, organic reagent or subsequent calcination.

DUMBBELL-SHAPED ZnO NANORODS: GROWTH AND CHARACTERIZATION

2011 ◽  
Vol 10 (01n02) ◽  
pp. 87-92 ◽  
Author(s):  
N. RAJKUMAR ◽  
M. PRABHU ◽  
K. RAMACHANDRAN
Keyword(s):  
Electron Microscopy ◽  
Large Scale ◽  
Zno Nanorods ◽  
Growth Direction ◽  
Hexagonal Structure ◽  
X Ray Diffraction ◽  
Step Method ◽  
Structural Morphology ◽  
Transmission Electron ◽  
One Step

A novel one-step method to synthesize large-scale uniform dumbbell-shaped ZnO nanorods is presented here. The structural morphology was investigated by using X-ray diffraction, scanning electron microscopy, and high-resolution transmission electron microscopy analyses. The prepared dumbbells with diameter of 300 nm and length of 1.5 μm possess hexagonal structure with preferable [001] growth direction. The UV–vis absorption spectra show blueshift for smaller nanoparticles and redshift for bigger dumbbell-shaped ZnO nanorods.

scholarly journals Large scale active-learning-guided exploration to maximize cell-free production

2019 ◽  
Author(s):  
Olivier Borkowski ◽  
Mathilde Koch ◽  
Agnès Zettor ◽  
Amir Pandi ◽  
Angelo Cardoso Batista ◽  
...  
Keyword(s):  
Active Learning ◽  
Large Scale ◽  
Protein Production ◽  
Critical Parameters ◽  
Step Method ◽  
Guided Exploration ◽  
Study Gene Expression ◽  
One Step ◽  
Combinatorial Space ◽  
Experimental Effort

AbstractLysate-based cell-free systems have become a major platform to study gene expression but batch-to-batch variation makes protein production difficult to predict. Here we describe an active learning approach to explore a combinatorial space of ~4,000,000 cell-free compositions, maximizing protein production and identifying critical parameters involved in cell-free productivity. We also provide a one-step-method to achieve high quality predictions for protein production using minimal experimental effort regardless of the lysate quality.

scholarly journals Water and mildew proof SiO2 & ZnO/silica sol superhydrophobic composite coating on a circuit board

RSC Advances ◽  
2021 ◽  
Vol 11 (35) ◽  
pp. 21862-21869
Author(s):  
Ziang Li ◽  
Changquan Li ◽  
Junfei Ou ◽  
Xinzuo Fang ◽  
Mengqi Cong ◽  
...  
Keyword(s):  
Composite Coating ◽  
Large Scale ◽  
Preparation Method ◽  
Silica Sol ◽  
Circuit Board ◽  
Step Method ◽  
Large Scale Preparation ◽  
One Step ◽  
Scale Preparation

A water and mildew proof SiO2 and ZnO/silica sol superhydrophobic composite coating was prepared by a one-step method and applied to a circuit board. The preparation method is simple and inexpensive, and convenient for large-scale preparation.

A Simple Synthesis of the Anticancer Drug Altretamine

2020 ◽  
Vol 17 (8) ◽  
pp. 628-630
Author(s):  
Vu Binh Duong ◽  
Pham Van Hien ◽  
Tran Thai Ngoc ◽  
Phan Dinh Chau ◽  
Tran Khac Vu
Keyword(s):  
Aqueous Solution ◽  
Anticancer Drug ◽  
Potassium Hydroxide ◽  
Large Scale ◽  
Synthesis Method ◽  
Practical Method ◽  
Cyanuric Chloride ◽  
Simple Synthesis ◽  
High Yield ◽  
One Step

A simple and practical method for the synthesis on a large scale of altretamine (1), a wellknown antitumor drug, has been successfully developed. The synthesis method involves the conversion of cyanuric chloride (2) into altretamine (1) by dimethylamination of 2 with an aqueous solution of 40% dimethylamine and potassium hydroxide in 1, -dioxan 4in one step to give altretamine (1) in high yield.

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