scholarly journals Expression of the Thermobifida fusca β-1,3-Glucanase in Yarrowia lipolytica and Its Application in Hydrolysis of β-1,3-Glucan from Four Kinds of Polyporaceae

Processes ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 56
Author(s):  
Wei-Lin Chen ◽  
Jo-Chieh Hsu ◽  
Chui-Li Lim ◽  
Cheng-Yu Chen ◽  
Chao-Hsun Yang
Keyword(s):  
Yarrowia Lipolytica ◽  
Enzymatic Reaction ◽  
Reducing Power ◽  
Molecular Size ◽  
Protein Band ◽  
Total Phenolic ◽  
Thermobifida Fusca ◽  
Gene Encoding ◽  
Pycnoporus Sanguineus ◽  
Phenolic Contents

The gene encoding a thermostable β-1,3-glucanase was cloned from Thermobifida fusca and expressed constitutively by Yarrowia lipolytica using plasmid pYLSC1. The expression level of the recombinant β-1,3-glucanase reached up to 270 U/mL in the culture medium. After a treatment with endo-β-N-acetyl-glucosaminidase H, the recombinant protein appeared as a single protein band, with a molecular size of approximately 66 kDa on the SDS-polyacrylamide gel. The molecular weight was consistent with the size predicted from the nucleotide sequence. The optimum temperature and pH of the transformant β-1,3-glucanase were 60 °C and pH 8.0, respectively. This β-1,3-glucanase was tolerant to 10% methanol, ethanol, and DMSO, retaining 70% activity. The enzyme markedly hydrolyzed Wolfiporia cocos and Pycnoporus sanguineus glucans. The DPPH and ABTS scavenging potential, reducing power and total phenolic contents of these two Polyporaceae hydrolysates, were significantly increased after 18 h of the enzymatic reaction. The present results indicate that T. fusca β-1,3-glucanase from Y. lipolytica transformant (pYLSC1-13g) hydrolyzes W. cocos and P. sanguineus glucans and improves the antioxidant potential of the hydrolysates.

scholarly journals Antioxidant Activity of Aqueous Extracts of Different Caffeine Products

2011 ◽  
Vol 39 (2) ◽  
pp. 117 ◽  
Author(s):  
Abd El-Moneim M.R. AFIFY ◽  
Emad A. SHALABY ◽  
Hossam Saad EL-BELTAGI
Keyword(s):  
Antioxidant Activity ◽  
Reducing Power ◽  
Hot Water ◽  
Total Phenolic ◽  
Aqueous Extracts ◽  
Power Method ◽  
Tea Leaves ◽  
Total Polyphenol ◽  
Water Extracts ◽  
Phenolic Contents

The antioxidant activity of water extracts (cold and hot) of six caffeine products were carried out. The extracts were screened for total polyphenol contents and antioxidant activity using DPPH, ABTS methods and reducing power method at 50 and 100 μg/ml after 15 min and 30 min using DPPH, ABTS BHA and Caffeine as standard compounds. The results indicated that, the hot water extracts for different caffeine products showed higher antioxidant activity than those of cold extracts and this activity was time and concentration dependent. In addition, the activity was higher against ABTS radical more than DPPH and reducing power methods. Also, there is a positive correlation between the antioxidant and reducing compounds presented in water extracts of different caffeine products. The results of HPLC showed that fresh tea leaves are rich in flavanol monomers known as catechins. The most abundant catechin derivatives in green tea are EGC, EGCG and GC. On the other hand EGCG and GC are major catechin derivative in different caffeine product except El-Fakher tea and Cacao. Generally, these beverages had high antioxidant capacities and total phenolic contents, and could be important dietary sources of antioxidant phenolic for prevention of diseases caused by oxidative stress.

scholarly journals Antioxidant Activity of Aspergillus fumigatus

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Daljit Singh Arora ◽  
Priyanka Chandra
Keyword(s):  
Antioxidant Activity ◽  
Aspergillus Fumigatus ◽  
Incubation Temperature ◽  
Reducing Power ◽  
Nitrogen Sources ◽  
Total Phenolic ◽  
Frap Assay ◽  
Carbon And Nitrogen ◽  
Phenolic Contents ◽  
Scavenging Effect

The antioxidant activity of Aspergillus fumigatus was assayed by different procedures and correlated with its extracellular total phenolic contents. Different physio-chemical parameters were optimized to enhance the activity. The culture grown under stationary conditions for 10 days at 25°C at pH 7 gave the best antioxidant activity. Statistical approaches demonstrated sucrose and NaNO3 to be the most suitable carbon and nitrogen sources, respectively. Response surface analysis showed 5% sucrose, 0.05% NaNO3, and incubation temperature of 35°C to be the optimal conditions for best expression of antioxidant activity. Under these conditions, the antioxidant potential assayed through different procedures was 89.8%, 70.1%, and 70.2% scavenging effect for DPPH radical, ferrous ion and nitric oxide ion, respectively. The reducing power showed an absorbance of 1.0 and FRAP assay revealed the activity of 60.5%. Extracellular total phenolic content and antioxidant activity as assayed by different procedures positively correlated.

Antioxidant and antimicrobial activities of four Astragalus species growing wild in Turkey

2018 ◽  
Vol 43 (4) ◽  
pp. 425-434 ◽  
Author(s):  
Sevil Albayrak ◽  
Onur Kaya
Keyword(s):  
Radical Scavenging ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Breast Cancer Cell Lines ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Phenolic Contents ◽  
Agar Well Diffusion Assay ◽  
Main Component ◽  
Mcf 7

Abstract Objective The objective of the present study was to investigate the phenolic compositions, antioxidant, antimicrobial and cytotoxic activities of four Astragalus species, two of which are endemic to Turkish flora. Methods The total phenolic and flavonoid contents of methanol extracts obtained from four Astragalus species were detected using Folin-Ciocalteu and aluminum chloride colorimetric assays. Their phenolic compositions were identified by Liquid chromatography–mass spectrometry (LC-MS). The antioxidant activity was assayed with phosphomolybdenum, 2,2-diphenyl-1-picrylhydrazyl radical scavenging (DPPH), hydrogen peroxide scavenging, β-carotene bleaching activity, ferric-ion reducing power (FRAP) and cupric ions (Cu2+) reducing antioxidant capacity (CUPRAC) methods. Antimicrobial activities of the extracts were studied by agar well-diffusion assay. The cytotoxic effects of the extracts on MCF-7 (human breast cancer cell lines) were determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) method. Results The extracts exerted moderate antioxidant and reducing activity with low phenolic contents. The main component in the extracts was determined as ferulic acid. The extracts demonstrated no antibacterial activity except P. aeruginosa. A. talasseus showed the highest cytotoxic activity on MCF-7 during 48 h. Conclusion It is believed that the results of this study will contribute to research recently increasing for the use of natural antioxidant and antimicrobial compounds in many industrial fields such as food, pharmacy and medicine.

scholarly journals Effect of Extraction Solvent/Technique on the Antioxidant Activity of Selected Medicinal Plant Extracts

Molecules ◽  
2009 ◽  
Vol 14 (6) ◽  
pp. 2167-2180 ◽  
Author(s):  
Bushra Sultana ◽  
Farooq Anwar ◽  
Muhammad Ashraf
Keyword(s):  
Antioxidant Activity ◽  
Organic Solvents ◽  
Reducing Power ◽  
Absolute Methanol ◽  
Terminalia Arjuna ◽  
Total Phenolic ◽  
Plant Materials ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Medicinal Plant Extracts

Theeffects of four extracting solvents [absolute ethanol, absolute methanol, aqueous ethanol (ethanol: water, 80:20 v/v) and aqueous methanol (methanol: water, 80:20 v/v)] and two extraction techniques (shaking and reflux) on the antioxidant activity of extracts of barks of Azadirachta indica, Acacia nilotica, Eugenia jambolana, Terminalia arjuna, leaves and roots of Moringa oleifera, fruit of Ficus religiosa,and leaves of Aloe barbadensis were investigated. The tested plant materials contained appreciable amounts of total phenolic contents (0.31-16.5 g GAE /100g DW), total flavonoid (2.63-8.66 g CE/100g DW); reducing power at 10 mg/mL extract concentration (1.36-2.91), DPPH. scavenging capacity (37.2-86.6%), and percent inhibition of linoleic acid (66.0-90.6%). Generally higher extract yields, phenolic contents and plant material antioxidant activity were obtained using aqueous organic solvents, as compared to the respective absolute organic solvents. Although higher extract yields were obtained by the refluxing extraction technique, in general higher amounts of total phenolic contents and better antioxidant activity were found in the extracts prepared using a shaker.

scholarly journals Phenolic Compounds and Antioxidant Activity of Castanopsis phuthoensis and Castanopsis grandicicatricata

2016 ◽  
Vol 55 ◽  
pp. 77-87 ◽  
Author(s):  
Phung Thi Tuyen ◽  
Do Tan Khang ◽  
Luong The Minh ◽  
Truong Ngoc Minh ◽  
Pham Thi Thu Ha ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Phenolic Extracts

In this study, total phenolic, flavonoid contents, antioxidant capacity, and phenolic compositions of Castanopsis phuthoensis and Castanopsis grandicicatricata (Fagaceae family) were investigated. It was found that bark extracts were rich of phenolic contents, whereas leaf extracts were abundant of flavonoids. The total phenolics varied from 11.20 to 35.47 mg gallic acid equivalent g-1 dry weight (DW), and the total flavonoids were from 2.24 to 12.55 mg rutin equivalent g-1 DW. The results of antioxidant activity showed that the DPPH (1,1-diphenyl-2-picryl-hydrazyl) radical scavenging activity of the free phenolic extracts were higher than the bound phenolic extracts. Regarding the reducing power and β-carotene bleaching assays, the free phenolic extracts showed remarkably strong antioxidant capacity that were similar to the levels of the standard BHT (dibutyl hydroxytoluene) did. It could be concluded that free phenolic extracts were more effective in antioxidant activities than bound phenolic extracts. A highly significant correlation between phenolic contents and antioxidant activity in extracts were observed. By HPLC analysis, seven phenolic acids were detected including gallic, p-hydroxybenzoic, vanillic, sinapic, p-coumaric, ellagic, and vanillin. Of which, gallic, ellagic, and sinapic acids were the most abundant compounds in the two species. The results suggest C. phuthoensis and C. grandicicatricata contain rich sources of natural antioxidants and phenolic compounds which are probably considered in pharmaceutical use.

scholarly journals The influence of varying cooking temperatures on the antioxidant properties of the aqueous extract of Piper guineense (Schumach & Thonn) seeds

2020 ◽  
Vol 12 (2) ◽  
pp. 211-217
Author(s):  
Babatunde Oso ◽  
Ige Olaoye
Keyword(s):  
Aqueous Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Property A ◽  
Piper Guineense ◽  
Phenolic Contents ◽  
Significant Difference ◽  
Dpph Scavenging

The study assessed the influence of varying cooking temperatures on the antioxidant properties of the aqueous extract of Piper guineense seeds. Different portions of the aqueous extract of P. guineense seeds were cooked at different temperatures which include 50 oC, 70 oC, and 90 oC for 10 minutes, while the remaining portion was allowed to stand for 10 minutes at room temperature of 29 oC and all were assessed for the evaluations of reducing power, radical scavenging capacities, and total phenolic contents. The results showed that there was a significant increase in the ferric reducing power at 90 oC, however, there was no significant difference between the raw and the cooked P. guineense seeds at 50 oC and 70 oC. In addition, the radical scavenging potential of the extract was highest at 70 oC, however, none of the cooked P. guineense seeds at the selected temperatures exhibited lower DPPH scavenging property. A similar trend was observed for the phenolic content of the extract with a significant reduction at 90 oC. The study suggests that cooking at 70 oC could enhance the antioxidant potentials of P. guineense seeds

scholarly journals Phytochemical analysis, total flavonoid, total phenolic contents and ferric reducing power of extracts from leaves and stem bark of Buddleja salviifolia

Food Research ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 221-227
Author(s):  
R.P. Matamane ◽  
S. Magama ◽  
M.K. Pillai ◽  
S.B. Mekbib
Keyword(s):  
Reducing Sugars ◽  
Reducing Power ◽  
Stem Bark ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Methanolic Extracts ◽  
Phytochemical Profiles

Chloroform, ethyl acetate and methanolic extracts from leaves and stem bark of Buddleja salviifolia were prepared. The phytochemical profiles, total flavonoid contents (TFC), total phenolic contents (TPC) and ferric reducing power of these extracts were studied. The presence of alkaloids, tannins, phlobatannins, saponins, sterols, flavonoids, coumarins, phenolics, terpenoids, quinones and reducing sugars were identified from these extracts. The TFC of these extracts was found to be in the range of 11.36±0.98 to 30.76±1.15 mg QE/g DW, respectively. The TPC of these extracts was found to be in the range of 175.00±0.41 to 465.27±2.65 mg GAE/g DW, respectively. Additionally, these extracts showed a weak to moderate ferric reducing power compared to ascorbic acid. B. salviifolia finds therapeutic applications in traditional medicine. Further studies are required to commercialize products from this plant.

scholarly journals Compositional diversity in essential oil of Ziziphora tenuior L. ecotypes

Genetika ◽  
2021 ◽  
Vol 53 (2) ◽  
pp. 717-727
Author(s):  
Hulya Dogan
Keyword(s):  
Essential Oil ◽  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Essential Oil Composition ◽  
Total Phenolic ◽  
Oil Composition ◽  
Phenolic Contents ◽  
Compositional Diversity

Present study reports a comparative screening of three ecotypes of Ziziphora tenuior L., sampled from three region with different elevation in middle and northeastern Turkey, based on their essential oil composition, total phenolic content and antioxidant capacities. Z. tenuior ecotypes showed twenty compounds, representing 89.18-91.44% of the essential oil (EOs) identified by GC-MS. The major components of EOs in ecotypes were pulegone (37.23-49.12%), and followed by 1,8-Cineole (2.26-7.78%), limonene (4.20-5.44%), ?-Caryophyllene (3.88-5.11%) and thymol (2.68-4.11%), respectively. Ecotypes showed variable pulegone content and it was increased with elevation increase. The total phenolic contents varied among ecotypes ranged from 21.13 to 27.50 mg of gallic acid equivalent/g EOs. Antioxidant capacity was determined in vitro using DPPH radical-scavenging and FRAP (Ferric reducing power) assays and expressed as concentration of each extract required to inhibit radical by 50% (IC50 and EC50) values that ranged from 0.80 to 0.97 mg/ml in DPPH and 0.42 to 0.55 mg/ml in FRAP assays, respectively. Our results indicated that antioxidant capacity changed among ecotypes. Taking into account the essential oil content, it seems that to select ecotype was the most appropriate to obtain more pulegone for this plant from its wild habitat.

scholarly journals Influence of Conventional Fermentation on Antioxidant Activity and Phenolic Contents of Two Common Dairy Products: Yogurt and Kefir

2020 ◽  
Vol 8 (6) ◽  
pp. 1277-1282
Author(s):  
Bilge Taşkın ◽  
Neriman Bağdatlıoğlu
Keyword(s):  
Phenolic Content ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Chelating Ability ◽  
Natural Foods ◽  
Dpph Radical Scavenging

During conventional fermentation of milk into yogurt and kefir, changes of antioxidant capacities (DPPH radical scavenging, ferrous chelating, reducing power, hydrogen peroxide/ H2O2 scavenging activity) and total phenolic content were investigated. Both products showed greater phenolic content, DPPH radical scavenging, and reducing power activities than was the case of their unfermented milk forms. But they were unable to scavenge H2O2. Chelating ability of milk increased by yogurt fermentation but decreased by kefir fermentation. In general, antioxidative capacity of milk improved throughout conventional yogurt and kefir fermentations and ripening periods (except chelating ability for kefir and H2O2 scavenging). This study is valuable since it evaluates the antioxidant power of yogurt and kefir produced by traditional fermentation with 5 different antioxidative approaches. In face of rapidly rising consumer demand for safe, healthy, functional but natural foods, revealed results might strengthen importance of conventional fermentation process and highlight the antioxidative contributions of mentioned products

scholarly journals Comparative Studies of Phytochemicals Analysis and in vitro Antioxidant Properties of Various Solvent Extracts of Sphaeranthus indicus Collected from three Different Climatic Zones of Central India

2021 ◽  
Vol 33 (9) ◽  
pp. 2237-2243
Author(s):  
Pooja N. Akhand ◽  
Veena Sharma ◽  
Anupam K. Pathak
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Central India ◽  
Total Phenolic ◽  
Climatic Zones ◽  
Phenolic Contents ◽  
Total Antioxidant ◽  
Sphaeranthus Indicus

In present study, the total phenolic, flavonoid, tannin contents and the antioxidant activity of various solvent extracts of Sphaeranthus indicus collected from three different regions of central India were assessed. Plants extracts were prepared using Soxhlation method, while the total phenols, flavonoids and tannins were measured by the spectrophotometric method. DPPH, metal chelating, nitric oxide, superoxide oxide scavenging activity and FRAP, reducing power, total antioxidant assays were also evaluated. The highest phenolic contents 268.22 GAE equivalent, flavonoids 441.33 QE equivalents and tannin content was 120.32 tannic acid equivalents (mg/g) obtained from ethanolic extracts of sample SIEE-1 compared to other two plants extract. Similarly, the highest flavonoid contents was observed in SIAE-1 aqueous extract and lowest in SIAE-2 and SIAE-3. Extract of SIEE-1 possessed maximum antioxidant potentiality and SIAE-2 shown the least antioxidant activity in all assays. It could be concluded that different agroclimatic conditions have effects on the total phenolics, flavonoids, tannin contents and antioxidant potentiality of S. indicus plant.

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