scholarly journals Thermophilic Anaerobic Digestion of Second Cheese Whey: Microbial Community Response to H2 Addition in a Partially Immobilized Anaerobic Hybrid Reactor

Processes ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 43
Author(s):  
Giuseppe Lembo ◽  
Silvia Rosa ◽  
Valentina Mazzurco Miritana ◽  
Antonella Marone ◽  
Giulia Massini ◽  
...  
Keyword(s):  
Microbial Community ◽  
Anaerobic Digestion ◽  
Cheese Whey ◽  
Community Response ◽  
Hybrid Reactor ◽  
Before And After ◽  
Methane Production Rate ◽  
Anaerobic Hybrid Reactor ◽  
Ch4 Concentration ◽  
H2 Addition

In this study, we investigated thermophilic (55 °C) anaerobic digestion (AD) performance and microbial community structure, before and after hydrogen addition, in a novel hybrid gas-stirred tank reactor (GSTR) implemented with a partial immobilization of the microbial community and fed with second cheese whey (SCW). The results showed that H2 addition led to a 25% increase in the methane production rate and to a decrease of 13% in the CH4 concentration as compared with the control. The recovery of methane content (56%) was reached by decreasing the H2 flow rate. The microbial community investigations were performed on effluent (EF) and on interstitial matrix (IM) inside the immobilized area. Before H2 addition, the Anaerobaculaceae (42%) and Lachnospiraceae (27%) families dominated among bacteria in the effluent, and the Thermodesulfobiaceae (32%) and Lachnospiraceae (30%) families dominated in the interstitial matrix. After H2 addition, microbial abundance showed an increase in the bacteria and archaea communities in the interstitial matrix. The Thermodesulfobiaceae family (29%)remained dominant in the interstitial matrix, suggesting its crucial role in the immobilized community and the SHA-31 family was enriched in both the effluent (36%) and the interstitial matrix (15%). The predominance of archaea Methanothermobacter thermoautrophicus indicated that CH4 was produced almost exclusively by the hydrogenotrophic pathway.

Effect of H2 addition on the microbial community structure of a mesophilic anaerobic digestion system

Energy ◽  
2020 ◽  
Vol 198 ◽  
pp. 117368 ◽  
Author(s):  
Xianpu Zhu ◽  
Liumeng Chen ◽  
Yichao Chen ◽  
Qin Cao ◽  
Xiaofeng Liu ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Anaerobic Digestion ◽  
Microbial Community Structure ◽  
H2 Addition

Microbial community evaluation of anaerobic granular sludge from a hybrid reactor treating pentachlorophenol by using fluorescence in situ hybridization

2003 ◽  
Vol 48 (6) ◽  
pp. 65-73 ◽  
Author(s):  
M.A.P. Montenegro ◽  
J.C. Araujo ◽  
R.F. Vazoller
Keyword(s):  
In Situ Hybridization ◽  
Microbial Community ◽  
Methanogenic Archaea ◽  
Granular Sludge ◽  
Hybrid Reactor ◽  
Anaerobic Granular Sludge ◽  
Bacterial Cells ◽  
Before And After ◽  
Community Evaluation

We used in situ hybridization with fluorescently labeled rRNA-targeted oligonucleotide probes concurrently with microscopic examinations and methane measurements to characterize the microbial community of an anaerobic hybrid reactor treating pentachlorophenol (PCP) with a mixture of fatty acids (propionic, butyric, acetic and lactic) and methanol. Archaeal cells detected with probe ARC915 prevailed in anaerobic granular sludge without and with the addition of PCP in a range of 2.0 to 21.0 mg/L to the reactor. This group accounted for 81 and 90% of the DAPI-stained cells before and after the addition of 21 mg/L of PCP, respectively. In these conditions, cells detected with the Methanosarcinales specific probe (MSMX860) were the only methanogenic Archaea found and accounted for 59 to 87.6% of the DAPI-stained cells. No cells were detected by the Methanomicrobiales (MG1200), Methanobacteriaceae (MB1174) and Methanococcaceae (MC1109) specific probes. Bacterial cells detected with probe EUB338 were found in very low numbers, which ranged from 5.7 to 1.0% of the DAPI-stained cells. This finding agrees with the scanning electron microscope examinations, in which cells morphologically resembling Methanosaeta and Methanosarcina were predominantly observed in the granular sludge. Results contributed to the investigation of the importance of the methanogens during PCP degradation.

scholarly journals Correction to: Anaerobic digestion of citrus industry effluents using an Anaerobic Hybrid Reactor

2018 ◽  
Vol 20 (7) ◽  
pp. 1399-1399 ◽  
Author(s):  
E. S. Rosas‑Mendoza ◽  
J. M. Méndez‑Contreras ◽  
A. Martínez‑Sibaja ◽  
N. A. Vallejo‑Cantú ◽  
A. Alvarado-Lassman
Keyword(s):  
Anaerobic Digestion ◽  
Hybrid Reactor ◽  
Citrus Industry ◽  
Anaerobic Hybrid Reactor

Anaerobic digestion of citrus industry effluents using an Anaerobic Hybrid Reactor

2017 ◽  
Vol 20 (7) ◽  
pp. 1387-1397 ◽  
Author(s):  
E. S. Rosas-Mendoza ◽  
J. M. Méndez-Contreras ◽  
A. Martínez-Sibaja ◽  
N. A. Vallejo-Cantú ◽  
A. Alvarado-Lassman
Keyword(s):  
Anaerobic Digestion ◽  
Hybrid Reactor ◽  
Citrus Industry ◽  
Anaerobic Hybrid Reactor

scholarly journals Assessment of Single- vs. Two-Stage Process for the Anaerobic Digestion of Liquid Cow Manure and Cheese Whey

Energies ◽  
2021 ◽  
Vol 14 (17) ◽  
pp. 5423
Author(s):  
Margarita Andreas Dareioti ◽  
Aikaterini Ioannis Vavouraki ◽  
Konstantina Tsigkou ◽  
Michael Kornaros
Keyword(s):  
Anaerobic Digestion ◽  
Production Rate ◽  
Methane Production ◽  
Cheese Whey ◽  
Biogas Production ◽  
Single Stage ◽  
Cow Manure ◽  
Two Stage ◽  
Methane Production Rate ◽  
Stage Process

The growing interest in processes that involve biomass conversion to renewable energy, such as anaerobic digestion, has stimulated research in this field in order to assess the optimum conditions for biogas production from abundant feedstocks, like agro-industrial wastes. Anaerobic digestion is an attractive process for the decomposition of organic wastes via a complex microbial consortium and subsequent conversion of metabolic intermediates to hydrogen and methane. The present study focused on the exploitation of liquid cow manure (LCM) and cheese whey (CW) as noneasily and easily biodegradable sources, respectively, using continuous stirred-tank reactors for biogas production, and a comparison was presented between single- and two-stage anaerobic digestion systems. No significant differences were found concerning LCM treatment, in a two-stage system compared to a single one, concluding that LCM can be treated by implementing a single-stage process, as a recalcitrant substrate, with the greatest methane production rate of 0.67 L CH4/(LR·d) at an HRT of 16 d. On the other hand, using the easily biodegradable CW as a monosubstrate, the two-stage process was considered a better treatment system compared to a single one. During the single-stage process, operational problems were observed due to the limited buffering capacity of CW. However, the two-stage anaerobic digestion of CW produced a stable methane production rate of 0.68 L CH4/(LR·d) or 13.7 L CH4/Lfeed, while the total COD was removed by 76%.

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