scholarly journals The Garden Candytuft (Iberis umbellata L.): At the Crossroad of Copper Accumulation and Glucosinolates

Processes ◽  
2020 ◽  
Vol 8 (9) ◽  
pp. 1116
Author(s):  
Mario Nikola Mužek ◽  
Dario Omanović ◽  
Azra Đulović ◽  
Franko Burčul ◽  
Sandra Svilović ◽  
...  
Keyword(s):  
Growth And Development ◽  
Copper Concentration ◽  
Sharp Decrease ◽  
Copper Accumulation ◽  
Glucosinolate Content ◽  
Copper Uptake ◽  
Zeolite Nax ◽  
Accumulator Plant ◽  
Accumulation Ability

The copper accumulation ability and its impact on the glucosinolate content of the garden candytuft were studied. Different copper sources (adsorbents or solution) were used. Generally, the seedlings copper uptake from the adsorbents was in the amount needed for its growth and development with the beneficial or no impact on the glucosinolate content. The lowest copper concentration was detected in the total seedlings biomass which grew in the humus with the addition of Cu-exchanged zeolite NaX (27.88 μg g−1 DW) having glucosinolate content of 9757.81 µg g−1 DW (23.86 µmol g−1 DW). The highest copper concentration among all the garden candytuft samples was detected in the seedlings watered with CuSO4∙5H2O solution (514.63 μg g−1 DW) with a sharp decrease of the glucosinolate content 3103.33 µg g−1 DW (7.59 µmol g−1 DW). Based on the results obtained, the garden candytuft can be considered as a copper accumulator plant.

scholarly journals Copper uptake potential of Philippine giant bamboo (Dendrocalamus asper) under varied initial copper concentration, water hardness and pH

2019 ◽  
Vol 268 ◽  
pp. 06005
Author(s):  
Jerwin Lawrence C.Go ◽  
Cynthia F. Madrazo ◽  
Aileen H. Orbecido ◽  
Ma. Ellenita G. de Castro ◽  
Lawrence P. Belo
Keyword(s):  
Copper Concentration ◽  
Water Hardness ◽  
Low Ph ◽  
Contaminated Water ◽  
Copper Uptake ◽  
Viable Option ◽  
Box Behnken Design ◽  
Construction Engineering ◽  
Dendrocalamus Asper ◽  
Copper Contaminated

Copper is a commonly used metal in construction, engineering, agriculture and water treatment. Consequently, increased copper concentrations resulting in adverse environmental effects is inevitable. Phytoremediation using Dendrocalamus asper or Philippine giant bamboo (PGB) is a viable option for treatment of copper-contaminated media, but their copper uptake potential remains largely unexplored. As such, the copper uptake of PGB was evaluated under varying environmental conditions, namely initial copper concentration, water hardness and pH.Six-month old propagules were planted in artificially contaminated water in order to determine the copper uptake after 16 days of treatment. Using a Box-Behnken design of experiment, it was found that both initial copper concentration and pH have significant and proportional effects on copper uptake. However, due to possible speciation and/or competition, the optimum copper uptake occurred at 20 ppm Cu and pH 5 (as opposed to 7). A mathematical equation, bearing an R2 = 0.7097, was constructed as a possible model for copper uptake of PGB to understand when PGB phytoremediation is most effective. At copper concentrations lower than 3.81 ppm, higher pH is beneficial to copper uptake and vice versa. Overall, phytoremediation using D. asper or PGB is effective especially at low pH and elevated copper concentrations.

Acclimation to Copper by Rainbow Trout,Salmo gairdneri: Biochemistry

1987 ◽  
Vol 44 (1) ◽  
pp. 105-111 ◽  
Author(s):  
Darrel Jon Laurén ◽  
D. G. McDonald
Keyword(s):  
Rainbow Trout ◽  
Specific Activity ◽  
Gill Tissue ◽  
Microsomal Protein ◽  
Whole Body ◽  
Salmo Gairdneri ◽  
Copper Accumulation ◽  
Copper Exposure ◽  
Copper Uptake ◽  
Liver Copper

Whole body, gill, and liver copper uptake, gill Na+-K+-ATPase specific activity, and gill and liver acid-soluble thiols (AST), glutathione, and cysteine of rainbow trout (Salmo gairdneri) were measured during 28 d of exposure to 55 μg copper∙L−1. Na+-K+-ATPase specific activity was inhibited by 33% within 24 h of copper exposure, but this was compensated by a significant increase in microsomal protein so that the total Na+-K+-ATPase activity per milligram of gill tissue returned to normal by day 14. There was no accumulation of copper and no increase in AST, glutathione, or cysteine in the gill. However, after 7 d of exposure, hepatic AST and glutathione had increased by about 2 times, and a sulfhydryl-rich, acid-soluble protein, tentatively identified as metallothionein, increased by 2.8 times. Copper accumulation was highest in the liver, but other tissues also accumulated copper.

The effect of rate of application of nitrogen fertilizer on soil copper uptake by barley under unirrigated conditions

1985 ◽  
Vol 104 (3) ◽  
pp. 583-587
Author(s):  
R. C. Tiwari ◽  
J. Adinarayana
Keyword(s):  
Nitrogen Fertilizer ◽  
Vegetative Growth ◽  
Copper Concentration ◽  
Field Experiments ◽  
Dilution Effect ◽  
Linear Increase ◽  
N Fertilizer ◽  
Copper Uptake ◽  
Barley Cultivars

SummaryField experiments were conducted on four barley cultivars (two hulled and two hullless) grown under unirrigated conditions in Varanasi, India, to observe the effect of rate of application of nitrogen on copper concentration and uptake in plants and soils. The copper concentration in both plants and soil decreased with age of crop because of the dilution effect of more vegetative growth. Varieties did not differ in copper concentration of plants but increasing application of nitrogen decreased copper concentration in the plants. Since the hulled varieties yielded more than the hull-less ones, they removed more copper from the soil. Similarly yield was increased linearly by the application of N fertilizer and this led to a linear increase in depletion of available copper.

scholarly journals Effects of Super Nutritional Hepatic Copper Accumulation on Hepatocyte Health and Oxidative Stress in Dairy Cows

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Jaimie M. Strickland ◽  
Doug Lyman ◽  
Lorraine M. Sordillo ◽  
Thomas H. Herdt ◽  
John P. Buchweitz
Keyword(s):  
Oxidative Stress ◽  
Liver Disease ◽  
Dairy Cows ◽  
Inductively Coupled Plasma ◽  
Copper Concentration ◽  
Tissue Level ◽  
Copper Accumulation ◽  
Serum Samples ◽  
Inductively Coupled ◽  
Hepatic Copper

Concerns regarding excessive hepatic copper concentrations in dairy cows have increased. The objective of this study was to determine the association of hepatic copper concentrations with evidence of liver disease. Blood and liver samples were collected at the time of slaughter in cull dairy cows (n=100). Liver samples were analyzed for copper using inductively coupled plasma mass spectrometry and crude fat using liquid-liquid extraction and gravimetry. Serum samples were analyzed for glutamate dehydrogenase,γ-glutamyltransferase, sorbitol dehydrogenase, aspartate aminotransferase activities, and bile acid concentrations. Liver samples were examined histologically for inflammation, fibrosis, and rhodanine staining. Animals were stratified by hepatic copper concentration and samples in the highest and lowest quintiles (Q5 and Q1) were evaluated for oxidative stress. Systemic indices of oxidative stress included serum reactive oxygen and nitrogen species (RONS) and total antioxidant potential (AOP). Tissue-level oxidative stress was assessed by immunohistochemistry using 4-hydroxynonenal (4HNE) and 3-nitrotyrosine (3NIT) stains to score the relative abundance and distribution of oxidized lipid and protein products, respectively. Mean hepatic copper concentration was 496.83μg/g and median 469.72μg/g and ranged from 70.56 to 1264.27μg/g dry tissue. No association was found between hepatic copper concentrations and clinicopathological or histological evidence of hepatic damage or dysfunction. There was a significant increase in the amount of IHC staining of 4HNE and 3NIT in Q5 compared with Q1. Moreover, the IHC staining mirrored the distribution of the copper-specific stain rhodanine. These results demonstrate that cows with elevated hepatic copper concentrations had no evidence of active liver disease but had increased hepatic oxidative stress.

Effect of chelators on copper metabolism and copper pools in mouse hepatocytes

1989 ◽  
Vol 256 (4) ◽  
pp. G667-G672
Author(s):  
H. J. McArdle ◽  
S. M. Gross ◽  
I. Creaser ◽  
A. M. Sargeson ◽  
D. M. Danks
Keyword(s):  
Metal Ion ◽  
Chelation Therapy ◽  
Copper Metabolism ◽  
Copper Accumulation ◽  
Copper Uptake ◽  
Mouse Hepatocytes ◽  
Copper Chelators ◽  
Copper Storage ◽  
And Storage

Disorders of copper storage are usually treated by chelation therapy. It is generally thought that the chelators act by mobilizing copper from the liver, hence allowing excretion in the urine. This paper has examined the effect of chelators on copper uptake and storage in mouse hepatocytes. Penicillamine, a clinically important chelator, does not block the uptake of copper or remove copper from hepatocytes. Two other copper chelators, sar and diamsar, which form very stable and kinetically inert Cu2+ complexes by encapsulating the metal ion in an organic cage, were shown to block copper accumulation by the cells and to remove up to 80% of cell-associated copper. They also removed most (approximately 80%) of the 64Cu accumulated by the cells in 30 min, but released only a small percentage (less than 20%) of that accumulated over 18 h. The results show that copper in the hepatocyte can be divided into at least two pools, an easily accessible one, and another, not removable even after long-term incubation with any of the chelators. Most of the copper normally found in the cell appeared to be associated with the former pool.

scholarly journals Copper Toxicity and Prediction Models of Copper Content in Leafy Vegetables

2019 ◽  
Vol 11 (22) ◽  
pp. 6215 ◽  
Author(s):  
Chiou ◽  
Hsu
Keyword(s):  
Copper Concentration ◽  
Prediction Models ◽  
Ethylenediaminetetraacetic Acid ◽  
Copper Toxicity ◽  
Toxic Metal ◽  
Leafy Vegetables ◽  
Copper Accumulation ◽  
Copper Level ◽  
Yield Reduction ◽  
Water Spinach

Copper (Cu), a toxic metal pollution found in the soil and water of industrialized areas, causes continuous issues for agriculture product contamination and human health hazards. However, information on copper phytotoxicity and its accumulation in vegetables is largely unknown. To evaluate the related agricultural loss and health risks, it is necessary to assess copper phytotoxicity and develop prediction models for copper concentration in vegetables. Here, we assess the growth performance and copper concentration of four leafy vegetables: Water spinach, amaranth, pakchoi, and garland chrysanthemum in copper-contaminated soil. The plant’s height and fresh weight is dramatically reduced when the soil copper concentration is over ~250 mg·kg−1. This yield reduction and copper accumulation are associated with an increase of soil copper concentration, suggesting high copper phytotoxicity levels in plants and soil. The prediction models of plant copper concentration were developed using multiple regressions based on one-step extractions of the soil copper as independent variables. One prediction model derived for amaranth copper using hydrochloric acid (HCl)-extractable and ethylenediaminetetraacetic acid (EDTA)-extractable copper from soil is able to describe 78.89% of the variance in the measured copper. As a result, the phytotoxic copper level for four leafy vegetables is revealed. Although the prediction models may not be universal, the predicted and phytotoxic copper levels are useful tools for evaluating vegetable yield and daily copper intake.

scholarly journals Regulation of Copper Toxicity by Candida albicansGPA2

2013 ◽  
Vol 12 (7) ◽  
pp. 954-961 ◽  
Author(s):  
Jennifer A. Schwartz ◽  
Karen T. Olarte ◽  
Jamie L. Michalek ◽  
Gurjinder S. Jandu ◽  
Sarah L. J. Michel ◽  
...  
Keyword(s):  
Copper Toxicity ◽  
Copper Homeostasis ◽  
Copper Resistance ◽  
Copper Accumulation ◽  
Copper Uptake ◽  
Α Subunit ◽  
Content Type ◽  
Copper Chelation ◽  
Excess Copper ◽  
Pka Pathway

ABSTRACTCopper is an essential nutrient that is toxic to cells when present in excess. The fungal pathogenCandida albicansemploys several mechanisms to survive in the presence of excess copper, but the molecular pathways that govern these responses are not completely understood. We report that deletion ofGPA2, which specifies a G-protein α subunit, confers increased resistance to excess copper and propose that the increased resistance is due to a combination of decreased copper uptake and an increase in copper chelation by metallothioneins. This is supported by our observations that agpa2Δ/Δ mutant has reduced expression of the copper uptake genes,CTR1andFRE7, and a marked decrease in copper accumulation following exposure to high copper levels. Furthermore, deletion ofGPA2results in an increased expression of the copper metallothionein gene,CRD2. Gpa2p functions upstream in the cyclic AMP (cAMP)-protein kinase A (PKA) pathway to govern hyphal morphogenesis. The copper resistance phenotype of thegpa2Δ/Δ mutant can be reversed by artificially increasing the intracellular concentration of cAMP. These results provide evidence for a novel role of the PKA pathway in regulation of copper homeostasis. Furthermore, the connection between the PKA pathway and copper homeostasis appears to be conserved in the pathogenCryptococcus neoformansbut not in the nonpathogenicSaccharomyces cerevisiae.

Effects of albumin and histidine on kinetics of copper transport by fibroblasts

1990 ◽  
Vol 259 (2) ◽  
pp. G212-G218 ◽  
Author(s):  
G. L. Waldrop ◽  
M. J. Ettinger
Keyword(s):  
Cell Types ◽  
Copper Transport ◽  
Copper Accumulation ◽  
Maximal Velocity ◽  
Copper Uptake ◽  
Inhibitory Effects ◽  
Serum Free ◽  
Intermediary Role ◽  
Free Media ◽  
Kinetics Of

The kinetics of copper transport by fibroblasts were examined and compared with earlier data with hepatocytes to determine the basis of rapid, preferential copper uptake by the liver. The Km and maximal velocity (Vmax) parameters for copper transport by fibroblasts in serum-free media were comparable to the parameters with hepatocytes. As with hepatocytes, albumin markedly inhibited initial rates of copper transport by fibroblasts. Although the only effect of histidine on copper transport by hepatocytes in serum-free media is a small increase in Km, histidine as His2Cu decreases the Vmax of copper transport fivefold with fibroblasts. Moreover, although histidine increases copper accumulation by hepatocytes when transport is inhibited by albumin, histidine further inhibits copper accumulation by fibroblasts when albumin is in the medium. Thus the inhibitory effects of histidine and albumin on copper transport by fibroblasts are additive. The data are consistent with an intermediary role for the His2Cu complex in copper transport. Copper is transported from His2Cu as the free ion, and copper transport is strictly passive with both cell types. The data suggest that rapid uptake by the liver is in part due to the ability of hepatocytes to transport copper from His2Cu more rapidly than other cell types.

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