scholarly journals Iron Oxide/Salicylic Acid Nanoparticles as Potential Therapy for B16F10 Melanoma Transplanted on the Chick Chorioallantoic Membrane

Processes ◽  
2020 ◽  
Vol 8 (6) ◽  
pp. 706
Author(s):  
Maria Cristina Predoi ◽  
Ion Mîndrilă ◽  
Sandra Alice Buteică ◽  
Ștefana Oana Purcaru ◽  
Dan Eduard Mihaiescu ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Iron Oxide ◽  
Local Development ◽  
Survival Rates ◽  
Chorioallantoic Membrane ◽  
Melanoma Cells ◽  
Cytotoxic Action ◽  
B16f10 Melanoma ◽  
Chick Chorioallantoic Membrane ◽  
Induced Apoptosis

Unfavorable prognoses and low survival rates are specific features of metastatic melanoma that justify the concern for the development of new therapeutic strategies. Lately, nanotechnology has become an attractive field of study due to recent advances in nanomedicine. Using a chick chorioallantoic membrane (CAM) implanted with xenografts harvested from C57BL/6 mice with B16F10 melanoma cells, we studied the effects of iron oxide nanoparticles functionalized with salicylic acid (SaMNPs) as a form of therapy on the local development of xenotransplants and CAM vessels. The SaMNPs induced an anti-angiogenic effect on the CAM vessels, which accumulated preferentially in the melanoma cells and induced apoptosis and extensive xenograft necrosis. As a result, this slowed the increase in the xenograft volume and reduced the melanoma cells’ ability to metastasize locally and distally. Further, we demonstrate the use of the chick CAM model as a tool for testing the action of newly synthesized nanocomposites on melanoma xenotransplants. The SaMNPs had a therapeutic effect on B16F10 melanoma due to the synergistic action of the two components of its structure: the coating of the salicylic acid with antiangiogenic and chemotherapeutic action and the core of iron oxides with cytotoxic action.

Proteomic Study Reveals a Co-occurrence of Gallic Acid-Induced Apoptosis and Glycolysis in B16F10 Melanoma Cells

2014 ◽  
Vol 62 (48) ◽  
pp. 11672-11680 ◽  
Author(s):  
Cheng Liu ◽  
Jen-Jie Lin ◽  
Zih-Yan Yang ◽  
Chi-Chu Tsai ◽  
Jue-Liang Hsu ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Melanoma Cells ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells ◽  
Proteomic Study ◽  
Induced Apoptosis

scholarly journals Phenotypic Switching of B16F10 Melanoma Cells as a Stress Adaptation Response to Fe3O4/Salicylic Acid Nanoparticle Therapy

2021 ◽  
Vol 14 (10) ◽  
pp. 1007
Author(s):  
Ion Mîndrilă ◽  
Andrei Osman ◽  
Bogdan Mîndrilă ◽  
Maria Cristina Predoi ◽  
Dan Eduard Mihaiescu ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Therapeutic Strategy ◽  
Aqueous Dispersion ◽  
Melanoma Cells ◽  
Phenotypic Switching ◽  
Treatment Modalities ◽  
B16f10 Melanoma ◽  
Adaptation Response ◽  
The Impact ◽  
Syngeneic Model

Melanoma is a melanocyte-derived skin cancer that has a high heterogeneity due to its phenotypic plasticity, a trait that may explain its ability to survive in the case of physical or molecular aggression and to develop resistance to therapy. Therefore, the therapy modulation of phenotypic switching in combination with other treatment modalities could become a common approach in any future therapeutic strategy. In this paper, we used the syngeneic model of B16F10 melanoma implanted in C57BL/6 mice to evaluate the phenotypic changes in melanoma induced by therapy with iron oxide nanoparticles functionalized with salicylic acid (SaIONs). The results of this study showed that the oral administration of the SaIONs aqueous dispersion was followed by phenotypic switching to highly pigmented cells in B16F10 melanoma through a cytotoxicity-induced cell selection mechanism. The hyperpigmentation of melanoma cells by the intra- or extracellular accumulation of melanic pigment deposits was another consequence of the SaIONs therapy. Additional studies are needed to assess the reversibility of SaIONs-induced phenotypic switching and the impact of tumor hyperpigmentation on B16F10 melanoma's progression and metastasis abilities.

scholarly journals Acetylcholinesterase Inhibitors Promote Angiogenesis in Chick Chorioallantoic Membrane and Inhibit Apoptosis of Endothelial Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Seyed Mohsen Mortazavian ◽  
Heydar Parsaee ◽  
Seyed Hadi Mousavi ◽  
Zahra Tayarani-Najaran ◽  
Ahmad Ghorbani ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Umbilical Vein ◽  
Chorioallantoic Membrane ◽  
Acetylcholinesterase Inhibitors ◽  
The Elderly ◽  
Vascular Changes ◽  
Beneficial Effects ◽  
Ache Inhibitors ◽  
Chick Chorioallantoic Membrane ◽  
Induced Apoptosis

Alzheimer’s disease (AD) is one of the most common causes of dementia in the elderly. Recently, a great attention has been paid to the possible role of vascular changes in the pathogenesis of AD. Reduced microvascular density and degeneration of the endothelium are of structural cerebrovascular changes in AD. Acetylcholinesterase (AChE) inhibitors are widely used for the improvement of AD symptoms. Until now, however, the effects of AChE inhibitors on vascular changes including angiogenesis and endothelial cell apoptosis are not fully understood. In the present work, the effects of three AChE inhibitors (donepezil, rivastigmine, and galantamine) were tested on H2O2-induced apoptosis in human umbilical vein endothelial cells (HUVECs) and on angiogenesis in chicken chorioallantoic membrane model. Incubation of HUVEC with H2O2led to a significant decrease in cell viability and an increase in the percentage of apoptotic cells. The tested drugs, at concentrations of 1–100 μM, significantly inhibited the H2O2-induced toxicity. Also, all donepezil, rivastigmine and galantamine significantly increased the number of vessels in the chorioallantoic membrane when injected into fertilized eggs. In conclusion, AChE inhibitors possess angiogenesis-accelerating properties and have antiapoptotic effects on endothelial cells. These effects of AChE inhibitors may be involved in their beneficial effects on AD.

Inhibitory Effects of Myelophycus simplex Papenfuss Methanol Extract on Melanogenesis in B16F10 Melanoma Cells

2017 ◽  
Vol 46 (1) ◽  
pp. 34-38
Author(s):  
Hyang Suk Kim ◽  
Ji Min Cheon ◽  
Da Hye Kwon ◽  
Eun Ok Choi ◽  
Min Ju Kim ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Melanoma Cells ◽  
Inhibitory Effects ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells
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