scholarly journals Improved Catalytic Properties of Thermomyces lanuginosus Lipase Immobilized onto Newly Fabricated Polydopamine-Functionalized Magnetic Fe3O4 Nanoparticles

Processes ◽  
2020 ◽  
Vol 8 (5) ◽  
pp. 629 ◽  
Author(s):  
Yanhong Bi ◽  
Zhaoyu Wang ◽  
Rui Zhang ◽  
Yihan Diao ◽  
Yaoqi Tian ◽  
...  

In this study, magnetic Fe3O4 nanoparticles coated with polydopamine possessing abundant amino groups (Fe3O4@PDA) were conveniently prepared, detailed, and characterized, and then firstly used as a supporting matrix for immobilizing Thermomyces lanuginosus lipase (Fe3O4@PDA@TLL). The effects of some crucial factors on the immobilization efficiency were investigated and the optimal protein loading and activity recovery were found to be 156.4 mg/g and 90.9%, respectively. Characterization studies revealed that Fe3O4@PDA@TLL displayed a broader pH and temperature adaptability as compared to the free TLL, which allows its use at wider ranges of reaction conditions. With regard to the stabilities, the immobilized TLL clearly displayed improved pH, thermal, and solvent tolerance stabilities compared to the free enzyme, suggesting that the biocompatible Fe3O4@PDA might be an outstanding material for immobilizing TLL and acting as alternative support for different enzymes.

2019 ◽  
Vol 43 (32) ◽  
pp. 12690-12697 ◽  
Author(s):  
Aleme Moslehi ◽  
Maaroof Zarei

The catalytic activity of magnetic Fe3O4 nanoparticles to promote the reduction of β-lactams containing nitroaryl groups to β-lactams containing aminoaryl groups in ethanol was reported. This methodology is convenient and green.


2021 ◽  
Vol 70 (5) ◽  
pp. 987-994
Author(s):  
A. M. Demin ◽  
A. V. Vakhrushev ◽  
M. S. Valova ◽  
A. S. Minin ◽  
D. K. Kuznetsov ◽  
...  

2009 ◽  
Vol 79-82 ◽  
pp. 505-508
Author(s):  
Li Li ◽  
H. Zhao ◽  
Wei Wang ◽  
F.F. Nie

The magnetic Fe3O4 nanoparticles had been synthesized by co-precipitation process and surface treatment by silane coupling agent (KH570). The magnetic Fe3O4/PMMA nanocomposite films were prepared by blend method, and the chemical structure, mechanical properties, surface morphology and the biocompatibility of the nanocomposite films were studied in this work. The magnetic Fe3O4 nanoparticles were well dispersed in the Fe3O4/PMMA nanocomposite films. The strength of the nanocomposite films, as well as the strain, decreased first and then increased with the increasing of the nanoparticles. The hemolytic ratio indicated that the nanocomposite films had a better blood compatibility.


2021 ◽  
pp. 129442
Author(s):  
Mariane Gonçalves Santos ◽  
Diailison Teixeira de Carvalho ◽  
Lucas Belga Caminitti ◽  
Bruna Bueno Alves de Lima ◽  
Marcello Henrique da Silva Cavalcanti ◽  
...  

2019 ◽  
Vol 475 ◽  
pp. 458-464 ◽  
Author(s):  
Soudabeh Arsalani ◽  
Eder J. Guidelli ◽  
Matheus A. Silveira ◽  
Carlos E.G. Salmon ◽  
Jefferson F.D.F. Araujo ◽  
...  

RSC Advances ◽  
2018 ◽  
Vol 8 (41) ◽  
pp. 22917-22923 ◽  
Author(s):  
Hira Fatima ◽  
Dae-Won Lee ◽  
Hyun Joong Yun ◽  
Kyo-Seon Kim

This paper describes a modified method to prepare monodisperse Fe3O4 magnetic nanoparticles with different shapes (cube, octahedron, and sphere).


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