scholarly journals Pollen Bee Aqueous Extract-Based Synthesis of Silver Nanoparticles and Evaluation of Their Anti-Cancer and Anti-Bacterial Activities

Processes ◽  
2020 ◽  
Vol 8 (5) ◽  
pp. 524
Author(s):  
Hanan M. Al-Yousef ◽  
Musarat Amina ◽  
Ali S. Alqahtani ◽  
Mohammed S. Alqahtani ◽  
Abdul Malik ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cell Lines ◽  
Aqueous Extract ◽  
Antibacterial Properties ◽  
Essential Amino Acids ◽  
Spectroscopic Techniques ◽  
Human Beings ◽  
Ag Nps ◽  
Anti Cancer ◽  
Ic50 Values

Bee pollens are rich source of essential amino acids and are often considered as complete food for human beings. Herein, we exploited the potential reducing abilities of Bee pollens extract for the eco-friendly preparation of silver nanoparticles (AgNPs-G). The resulting NPs were characterized using a combination of microscopic and spectroscopic techniques. The analyses confirm the formation of spherical Ag NPs. AgNPs-G obtained from the aqueous extract of bee pollens was used to study their antibacterial properties against Gram-positive and Gram-negative microbes using the Minimum Inhibitory Concentration 50 (MIC50) method. The antibacterial properties of AgNPs-G were compared to the properties of chemically synthesized Ag NPs (AgNPs-C) using sodium borohydride as a reducing agent. The green synthesized nanoparticles (AgNPs-G) exhibited a better antibacterial activity against most of the studied strains when compared to the chemically synthesized Ag NPs (AgNPs-C). In addition, the anti-cancer activity of Ag NPs was also studied against human liver and breast carcinoma cell lines by applying MTT-assay. The Ag NPs demonstrated considerable anticancer activity against the studied cell lines and exhibited high IC50 values in both MCF-7 and HepG2 cell lines.

scholarly journals Silver Nanoparticles: Cytotoxic and Apoptotic Activity on HT-29 and A549 Cell Lines

2018 ◽  
Vol 1 (4) ◽  
pp. 1-6 ◽  
Author(s):  
Abdolhossien Miri ◽  
Mina Sarani
Keyword(s):  
Silver Nanoparticles ◽  
Cell Lines ◽  
A549 Cell ◽  
A549 Cell Line ◽  
Tunel Method ◽  
Ag Nps ◽  
Anti Cancer ◽  
Cancer Agent ◽  
Apoptotic Activity ◽  
Ht 29

Silver nanoparticles (Ag-NPs) are versatile materials with a broad range of applications in various fields such as cancer therapy, drug delivery. In this work, cytotoxic and apoptotic activities of silver nanoparticles was evaluation against lung (A549) and colon (HT-29) cell lines. The cytotoxic activity of nanoparticles was performed by MTT assay, while their apoptotic activity was tested through TUNEL method. The results of MTT of A549 have illustrate that fifty percent of cells destruction in concentrations more than 250 µg/ml of Ag-NPs. Apoptotic results of nanoparticles have shown more than fifty percent of apoptosis on A549 cell line. HT-29 display full apoptosis at concentrations more than 500 µg/ml. It seems that synthesized Ag-NPs by using P. farcta extract can be candidate as anti-cancer agent in treatment many cancers through creating or discovering new drug forms

Silver nanoparticles induce cytotoxicity, but not cell transformation or genotoxicity on Balb3T3 mouse fibroblasts

2013 ◽  
Vol 14 (1-2) ◽  
pp. 49-60 ◽  
Author(s):  
Francesca Broggi ◽  
Jessica Ponti ◽  
Guido Giudetti ◽  
Fabio Franchini ◽  
Vicki Stone ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cell Transformation ◽  
Micronucleus Test ◽  
Chemical Properties ◽  
Neoplastic Transformation ◽  
Research Centre ◽  
Ag Nps ◽  
Joint Research ◽  
Ic50 Values ◽  
Average Size

AbstractSilver nanoparticles (Ag NPs) are one of the most common nanomaterials present in nanotechnology-based products. Here, the physical chemical properties of Ag NPs suspensions of 44 nm, 84 nm and 100 nm sizes synthesized in our laboratory were characterized. The NM-300 material (average size of 17 nm), supplied by the Joint Research Centre Nanomaterials Repository was also included in the present study. The Ag NPs potential cytotoxicity was tested on the Balb3T3 cell line by the Colony Forming Efficiency assay, while their potential morphological neoplastic transformation and genotoxicity were tested by the Cell Transformation Assay and the micronucleus test, respectively. After 24 h of exposure, NM-300 showed cytotoxicity with an IC50 of 8 µM (corresponding to 0.88 µg/mL) while for the other nanomaterials tested, values of IC50 were higher than 10 µM (1.10 µg/mL). After 72 h of exposure, Ag NPs showed size-dependent cytotoxic effect with IC50 values of 1.5 µM (1.16 µg/mL) for NM-300, 1.7 µM (1.19 µg/mL) for Ag 44 nm, 1.9 µM (0.21 µg/mL) for Ag 84 nm and 3.2 µM (0.35 µg/mL) for Ag 100 nm. None of the Ag NPs tested was able to induce either morphological neoplastic transformation or micronuclei formation.

scholarly journals Synthetic (E)-3-Phenyl-5-(phenylamino)-2-styryl-1,3,4-thiadiazol-3-ium Chloride Derivatives as Promising Chemotherapy Agents on Cell Lines Infected with HTLV-1

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2537
Author(s):  
Danilo Sousa-Pereira ◽  
Thais Silva de Oliveira ◽  
Rojane O. Paiva ◽  
Otávio Augusto Chaves ◽  
José C. Netto-Ferreira ◽  
...  
Keyword(s):  
Molecular Docking ◽  
T Cell ◽  
Cell Lines ◽  
Pharmacokinetic Profile ◽  
Biological Evaluation ◽  
Spectroscopic Techniques ◽  
Time Resolved ◽  
Adult T Cell Leukemia ◽  
Human T Cell ◽  
Ic50 Values

Synthesis of four compounds belonging to mesoionic class, (E)-3-phenyl-5-(phenylamino)-2-styryl-1,3,4-thiadiazol-3-ium chloride derivatives (5a–d) and their biological evaluation against MT2 and C92 cell lines infected with human T-cell lymphotropic virus type-1 (HTLV-1), which causes adult T-cell leukemia/lymphoma (ATLL), and non-infected cell lines (Jurkat) are reported. The compounds were obtained by convergent synthesis under microwave irradiation and the cytotoxicity was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. Results showed IC50 values of all compounds in the range of 1.51–7.70 μM in HTLV-1-infected and non-infected cells. Furthermore, it was observed that 5b could induce necrosis after 24 h for Jurkat and MT2 cell lines. The experimental (fluorimetric method) and theoretical (molecular docking) results suggested that the mechanism of action for 5b could be related to its capacity to intercalate into DNA. Moreover, the preliminary pharmacokinetic profile of the studied compounds (5a–d) was obtained through human serum albumin (HSA) binding affinity using multiple spectroscopic techniques (circular dichroism, steady-state and time-resolved fluorescence), zeta potential and molecular docking calculations. The interaction HSA:5a–d is spontaneous and moderate (Ka ~ 104 M−1) via a ground-state association, without significantly perturbing both the secondary and surface structures of the albumin in the subdomain IIA (site I), indicating feasible biodistribution in the human bloodstream.

scholarly journals Microwave-Assisted Green Synthesis of Silver Nanoparticles Using Juglans regia Leaf Extract and Evaluation of Their Physico-Chemical and Antibacterial Properties

Antibiotics ◽  
2018 ◽  
Vol 7 (3) ◽  
pp. 68 ◽  
Author(s):  
Mahsa Eshghi ◽  
Hamideh Vaghari ◽  
Yahya Najian ◽  
Mohammad Najian ◽  
Hoda Jafarizadeh-Malmiri ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Juglans Regia ◽  
Antibacterial Properties ◽  
Infrared Analysis ◽  
Ag Nps ◽  
Stabilizing Agents ◽  
Agno3 Solution ◽  
Transmission Electron ◽  
Vis Spectroscopy

Silver nanoparticles (Ag NPs) were synthesized using Juglans regia (J. regia) leaf extract, as both reducing and stabilizing agents through microwave irradiation method. The effects of a 1% (w/v) amount of leaf extract (0.1–0.9 mL) and an amount of 1 mM AgNO3 solution (15–25 mL) on the broad emission peak (λmax) and concentration of the synthesized Ag NPs solution were investigated using response surface methodology (RSM). Fourier transform infrared analysis indicated the main functional groups existing in the J. regia leaf extract. Dynamic light scattering, UV-Vis spectroscopy and transmission electron microscopy were used to characterize the synthesized Ag NPs. Fabricated Ag NPs with the mean particle size and polydispersity index and maximum concentration and zeta potential of 168 nm, 0.419, 135.16 ppm and −15.6 mV, respectively, were obtained using 0.1 mL of J. regia leaf extract and 15 mL of AgNO3. The antibacterial activity of the fabricated Ag NPs was assessed against both Gram negative (Escherichia coli) and positive (Staphylococcus aureus) bacteria and was found to possess high bactericidal effects.

scholarly journals Silver Nanoparticles Induce Mitochondrial Protein Oxidation in Lung Cells Impacting Cell Cycle and Proliferation

Antioxidants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 552 ◽  
Author(s):  
Reetta J. Holmila ◽  
Stephen A. Vance ◽  
S. Bruce King ◽  
Allen W. Tsang ◽  
Ravi Singh ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Silver Nanoparticles ◽  
Ionizing Radiation ◽  
Cell Lines ◽  
Protein Oxidation ◽  
Mitochondrial Protein ◽  
Antibacterial Properties ◽  
Dependent Manner ◽  
Mitochondrial Redox State ◽  
And Function

Silver nanoparticles (AgNPs) are widely used nanomaterials in both commercial and clinical biomedical applications, due to their antibacterial properties. AgNPs are also being explored for the treatment of cancer in particular in combination with ionizing radiation. In this work, we studied the effects of AgNPs and ionizing radiation on mitochondrial redox state and function in a panel of lung cell lines (A549, BEAS-2B, Calu-1 and NCI-H358). The exposure to AgNPs caused cell cycle arrest and decreased cell proliferation in A549, BEAS-2B and Calu-1, but not in NCI-H358. The mitochondrial reactive oxygen species (ROS) and protein oxidation increased in a time- and dose-dependent manner in the more sensitive cell lines with the AgNP exposure, but not in NCI-H358. While ionizing radiation also induced changes in the mitochondrial redox profiles, in general, these were not synergistic with the effects of AgNPs with the exception of NCI-H358 and only at a higher dose of radiation.

scholarly journals Alendronic Acid as Ionic Liquid: New Perspective on Osteosarcoma

Pharmaceutics ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 293 ◽  
Author(s):  
Sónia Teixeira ◽  
Miguel M. Santos ◽  
Maria H. Fernandes ◽  
João Costa-Rodrigues ◽  
Luís C. Branco
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Alendronic Acid ◽  
Biological Fluids ◽  
Bone Cancer ◽  
Cell Types ◽  
Spectroscopic Techniques ◽  
Osteosarcoma Cell ◽  
Organic Salts ◽  
Ic50 Values

Herein the quantitative synthesis of eight new mono- and dianionic Organic Salts and Ionic Liquids (OSILs) from alendronic acid (ALN) is reported by following two distinct sustainable and straightforward methodologies, according to the type of cation. The prepared ALN-OSILs were characterized by spectroscopic techniques and their solubility in water and biological fluids was determined. An evaluation of the toxicity towards human healthy cells and also human breast, lung and bone (osteosarcoma) cell lines was performed. Globally, it was observed that the monoanionic OSILs showed lower toxicity than the corresponding dianionic structures to all cell types. The highest cytotoxic effect was observed in OSILs containing a [C2OHMIM] cation, in particular [C2OHMIM][ALN]. The latter showed an improvement in IC50 values of ca. three orders of magnitude for the lung and bone cancer cell lines as well as fibroblasts in comparison with ALN. The development of OSILs with high cytotoxicity effect towards the tested cancer cell types, and containing an anti-resorbing molecule such as ALN may represent a promising strategy for the development of new pharmacological tools to be used in those pathological conditions.

scholarly journals Fabrication, Characterization, and Antibacterial Properties of Electrospun Membrane Composed of Gum Karaya, Polyvinyl Alcohol, and Silver Nanoparticles

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Vinod Vellora Thekkae Padil ◽  
Nhung H. A. Nguyen ◽  
Alena Ševců ◽  
Miroslav Černík
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Polyvinyl Alcohol ◽  
Food Packaging ◽  
Chemical Reduction ◽  
Antibacterial Properties ◽  
Hydroxyl Groups ◽  
Ag Nps ◽  
Gum Karaya ◽  
Nanofiber Membranes

Gum karaya (GK), a natural hydrocolloid, was mixed with polyvinyl alcohol (PVA) at different weight ratios and electrospun to produce PVA/GK nanofibers. An 80 : 20 PVA/GK ratio produced the most suitable nanofiber for further testing. Silver nanoparticles (Ag-NPs) were synthesised through chemical reduction of AgNO3(at different concentrations) in the PVA/GK solution, the GK hydroxyl groups being oxidised to carbonyl groups, and Ag+cations reduced to metallic Ag-NPs. These PVA/GK/Ag solutions were then electrospun to produce nanofiber membranes containing Ag-NPs (Ag-MEMs). Membrane morphology and other characteristics were analysed using scanning electron microscopy coupled with energy dispersive X-ray analysis, transmission electron microscopy, and UV-Vis and ATR-FTIR spectroscopy. The antibacterial activity of the Ag-NP solution and Ag-MEM was then investigated against Gram-negativeEscherichia coliandPseudomonas aeruginosaand Gram-positiveStaphylococcus aureus. Our results show that electrospun nanofiber membranes based on natural hydrocolloid, synthetic polymer, and Ag-NPs have many potential uses in medical applications, food packaging, and water treatment.

scholarly journals Biosynthesis and Characterization of Silver Nanoparticles from Bitter Melon (Momordica charantia) Fruit and Seed Extract and their Antimicrobial Activity

2021 ◽  
Vol 2 (1) ◽  
pp. 1-11
Author(s):  
Umme Ruman ◽  
Poonah Kia
Keyword(s):  
Silver Nanoparticles ◽  
Average Particle Size ◽  
Antibacterial Properties ◽  
Seed Extract ◽  
Food Preservation ◽  
Momordica Charantia ◽  
Average Particle ◽  
Ag Nps ◽  
Fruit Extract ◽  
Biotechnological Approach

Momordica charantia is a phenolic rich vegetable. In this study, the fruits and seeds extract of M. charantia were used to synthesize silver nanoparticles (Ag NPs) using biotechnological approach. Structural, morphological, and antimicrobial properties of the synthesized Ag-NPs were characterized using UV/Vis Spectrophotometry, Dynamic Light Scattering (DLS), High Resolution Transmission Electronics Microscopy (HRTEM), Field Emission Scanning Electron Microscopy (FESEM), Fourier Transform Infrared Spectroscopy (FTIR) and X-Ray diffraction (XRD). In DLS, the average particle size of Ag-NPs was found 17.5 ± 2.1 nm and 18.3 ± 1.9 nm using seed and fruit extract, respectively. HRTEM has revealed their spherical structure for both seed and fruit extract of M. charantia. FESEM images found Ag-NPs with the size between ~20 and ~35 nm. The Ag NPs exhibited Surface Plasmon Resonance (SPR) centered at 405 nm for seed extract and 402 nm for fruit extract using a UV–visible spectrophotometer. FT-IR results showed phenolic and carbohydrate compounds involved in the synthesis of the Ag NPs. Furthermore, the synthesized Ag NPs has found highly rich in antibacterial properties against Escherichia coli and Pseudomonas aeruginosa bacterium. Thus, bioconversion of Ag NPs by M. charantia could be employed as a potential antibacterial source to eliminate pathogenic microorganisms from agricultural and food preservation industry.

scholarly journals Green Synthesis of Silver Nanoparticles Using Grewia optiva Leaf Aqueous Extract and Isolated Compounds as Reducing Agent and Their Biological Activities

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Muhammad Iftikhar ◽  
Muhammad Zahoor ◽  
Sumaira Naz ◽  
Nausheen Nazir ◽  
Gaber El-Saber Batiha ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Aqueous Extract ◽  
Hair Growth ◽  
Leaf Extract ◽  
Biological Activities ◽  
Ag Nps ◽  
X Ray ◽  
Leaf Aqueous Extract ◽  
Antioxidant Agents ◽  
Grewia Optiva

In this study, an attempt was made to synthesize silver nanoparticles (Ag-NPs) using Grewia optiva leaf extract and isolated compounds. The bioreductant capacity of Grewia optiva leaf extract for the synthesis of Ag-NPs was assessed using various confirmatory techniques like thermogravimetric analysis (TGA), particle size analysis (PSA), energy-dispersive X-ray (EDX), X-ray diffraction (XRD) analysis, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and UV-Visible spectroscopy. The presence of various bioactive compounds in leaf aqueous extract was confirmed through HPLC analysis, and 8 compounds were identified among the different peaks present in the chromatogram. Biopotencies like antioxidant, antibacterial, and effect on hair growth were determined for extract and NPs. Antioxidant capacities were assessed through standard ABTS and DPPH methods. The antibacterial potential was evaluated in terms of zone of inhibition, minimum bactericidal concentration, and minimum inhibitory concentration of the Ag-NPs and the leaf extract against selected strains of bacteria, whereas the effect on growth of rabbit hair was studied through topical treatment for a specific period of time. Better antibacterial and DPPH and ABTS free radical inhibition was observed for the formulated Ag-NPs as compared to leaf extract. The previously isolated eight compounds from this plant’s chloroform and ethyl acetate extracts were also tested for their bioreductant capacities. Out of them, the highest amount of precipitates was obtained with compound VII ((2,5-dihydroxyphenyl)-3 ′ ,6 ′ ,8 ′ -trihydroxyl-4H-chromen-4 ′ -one). The study implies that the biogenically engineered nanoscale particles could have promising biological activities in comparison to parental extract and they need to be investigated further as potential therapeutic agents to be used as antibacterial and antioxidant agents and for hair growth enhancement.

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