scholarly journals Concentration of Lipase from Aspergillus oryzae Expressing Fusarium heterosporum by Nanofiltration to Enhance Transesterification

Processes ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 450 ◽  
Author(s):  
Hans Wijaya ◽  
Kengo Sasaki ◽  
Prihardi Kahar ◽  
Emmanuel Quayson ◽  
Nova Rachmadona ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Aspergillus Oryzae ◽  
Membrane Separation ◽  
Acid Methyl Ester ◽  
Total Activity ◽  
Biodiesel Production ◽  
Nanofiltration Membrane ◽  
Recombinant Lipase ◽  
Fusarium Heterosporum ◽  
Commercial Lipase

Nanofiltration membrane separation is an energy-saving technology that was used in this study to concentrate extracellular lipase and increase its total activity for biodiesel production. Lipase was produced by recombinant Aspergillus oryzae expressing Fusarium heterosporum lipase (FHL). A sulfonated polyethersulfone nanofiltration membrane, NTR-7410, with a molecular weight cut-off of 3 kDa was used for the separation, because recombinant lipase has a molecular weight of approximately 20 kDa, which differs from commercial lipase at around 30 kDa for CalleraTM Trans L (CalT). After concentration via nanofiltration, recombinant lipase achieved a 96.8% yield of fatty acid methyl ester (FAME) from unrefined palm oil, compared to 50.2% for CalT in 24 h. Meanwhile, the initial lipase activity (32.6 U/mL) of recombinant lipase was similar to that of CalT. The composition of FAME produced from recombinant concentrated lipase, i.e., C14:1, C16:0, C18:0, C18:1 cis, and C18:2 cis were 0.79%, 34.46%, 5.41%, 45.90%, and 12.46%, respectively, after transesterification. This FAME composition, even after being subjected to nanofiltration, was not significantly different from that produced from CalT. This study reveals the applicability of a simple and scalable nanofiltration membrane technology that can enhance enzymatic biodiesel production.

scholarly journals Primitive techno-economic study of bio-diesel and bio-active compound production from microalgae

2020 ◽  
Vol 44 (1) ◽  
Author(s):  
Sayeda M. Abdo ◽  
Guzine I. El Diwani ◽  
Kamel M. El-Khatib ◽  
Sanaa A. Abo El-Enin ◽  
Mohammed I. El-Galad ◽  
...  
Keyword(s):  
Active Compound ◽  
Municipal Wastewater ◽  
Scenedesmus Obliquus ◽  
Cetane Number ◽  
Treatment Plant ◽  
Acid Methyl Ester ◽  
Biodiesel Production ◽  
High Rate ◽  
Total Phenolic ◽  
Microscopical Examination

Abstract Background Microalgae cells can be identified as a potential source for new and renewable energy. The economic investigation for biodiesel and bio-active compound production from the microalgae community (Bloom), which are collected from the high rate algal pond (HRAP) constructed to treat municipal wastewater at Zenin wastewater treatment plant, Giza, was the main target of study. Results The microscopical examination showed that Scenedesmus obliquus is the dominant species. The total carotenoids were extracted using jojoba oil and determined by high-performance liquid chromatography (HPLC) to reach 81.44 μg/g. The biodiesel production through acid transesterification reaction recorded 70.6% of fatty acid methyl ester content with high cetane number (44) and low acid value. Such results prove that the obtained biodiesel has better ignition quality. The total phenolic and flavonoid compounds have been derived from the remaining biomass to give 5.36 ± 0.03 and 1.50 ± 0.19 mg/g respectively. Finally, total proteins and carbohydrates content in algal cells were recorded 54.3 and 1.5 mg/g successively Conclusion The preliminary economic evaluation showed that the production of biodiesel and carotenoids from the microalgae growing in municipal wastewater can be considered, as a techno-economic feasible process.

Calcium Methoxide Synthesis from Quick Lime Using as Solid Catalyst in Refined Palm Oil Biodiesel Production

2013 ◽  
Vol 834-836 ◽  
pp. 550-554 ◽  
Author(s):  
Warakom Suwanthai ◽  
Vittaya Punsuvon ◽  
Pilanee Vaithanomsat
Keyword(s):  
Palm Oil ◽  
Acid Methyl Ester ◽  
Biodiesel Production ◽  
Molar Ratio ◽  
Catalyst Loading ◽  
Solid Catalyst ◽  
Solid Base ◽  
Quick Lime ◽  
X Ray ◽  
Refined Palm Oil

In this research, calcium methoxide was synthesized as solid base catalyst from quick lime for biodiesel production. The catalyst was further characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), attenuated total reflection fourier transform (ATR-FTIR) and Energy-dispersive X-ray spectroscopies (EDX) to evaluate its performance. The transesterification of refined palm oil using calcium methoxide and the process parameters affecting the fatty acid methyl ester (FAME) content such as catalyst concentration, methanol:oil molar ratio and reaction time were investigated. The results showed that the FAME content at 97% was achieved within 3 h using 3 %wt catalyst loading, 12:1 methanol:oil molar ratio and 65 °C reaction temperature. The result of FAME suggested calcium methoxide was the promising solid catalyst for substitution of the conventional liquid catalyst.

scholarly journals A Robust Two-Step Process for the Efficient Conversion of Acidic Soybean Oil for Biodiesel Production

Catalysts ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 527 ◽  
Author(s):  
Gaojian Ma ◽  
Lingmei Dai ◽  
Dehua Liu ◽  
Wei Du
Keyword(s):  
Fatty Acid ◽  
Soybean Oil ◽  
Lower Cost ◽  
Immobilized Lipase ◽  
Acid Methyl Ester ◽  
Acid Value ◽  
Catalytic Performance ◽  
Raw Material ◽  
Biodiesel Production ◽  
Negative Effect

Acidic oil, which is easily obtained and with lower cost, is a potential raw material for biodiesel production. Apart from containing large quantity of FFAs (free fatty acids), acidic oil usually contains some amount of inorganic acid, glycerides and some other complex components, leading to complicated effect on lipase’s catalytic performance. Exploring the efficient process of converting acidic oil for biodiesel production is of great significance to promote the use of acidic oil. A two-step conversion process for acidic soybean oil was proposed in this paper, where sulfuric acid-mediated hydrolysis was adopted first, then the hydrolyzed free fatty acid, collected from the upper oil layer was further subject to the second-step esterification catalyzed by immobilized lipase Novozym435. Through this novel process, the negative effect caused by harmful impurities and by-product glycerol on lipase was eliminated. A fatty acid methyl ester (FAME) yield of 95% could be obtained with the acid value decreased to 4 mgKOH/g from 188 mgKOH/g. There was no obvious loss in lipase’s activity and a FAME yield of 90% could be maintained with the lipase being repeatedly used for 10 batches. This process was found to have a good applicability to different acidic oils, indicating it has great prospect for converting low quality oil sources for biodiesel preparation.

scholarly journals Preliminary Study on Effect of Hydrothermal Temperature during TiO2 Synthesis on the Biodiesel Production from Waste Cooking Oil

2021 ◽  
Vol 50 (6) ◽  
pp. 1685-1695
Author(s):  
Nurashina Abdul Rahman ◽  
Anita Ramli ◽  
Chong Fai Kait
Keyword(s):  
Photocatalytic Activity ◽  
Acid Methyl Ester ◽  
Textural Properties ◽  
Rutile Phase ◽  
Waste Cooking Oil ◽  
Biodiesel Production ◽  
Hydrothermal Temperature ◽  
Titanium Butoxide ◽  
Cooking Oil ◽  
Type Iv

In the present work, effect of hydrothermal temperature from 120 to 160 °C on TiO2 physicochemical properties as well as its photocatalytic activity towards biodiesel production using waste cooking oil (WCO) was investigated. TiO2was synthesized via hydrothermal method using Titanium butoxide, Ti(OBu)4 as the precursor and nitric acid, HNO3 as the peptizing agent. Next, the synthesized photocatalyst was dried at 60 °C for 24 h and later calcined at 400 °Cfor 2 h. The synthesized TiO2 was characterized using X-ray diffraction (XRD) and Burnauer- Emmet- Teller (BET) to determine their crystallinity and textural properties. Results showed that all synthesized TiO2 have a mixture of anatase and rutile phase and N2 adsorption- desorption isotherm for all catalyst possess Type IV isotherm according to IUPACclassification with hysteresis loop of type H1. Then, all the synthesized catalysts were tested for biodiesel production using esterified waste cooking oil under visible light irradiation for 1 h and 10 min. Percentage of fatty acid methyl ester (FAMEs) present in the synthesized biodiesel was determined using gas chromatography with flame ionization detector (GC-FID). The synthesized catalyst (T24_160) showed a good photocatalytic activity as the percentage of biodiesel yield was higher (3.41%) compared to the other catalyst.

scholarly journals Immobilization of Eversa Lipase on Octyl Agarose Beads and Preliminary Characterization of Stability and Activity Features

Catalysts ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 511 ◽  
Author(s):  
Sara Arana-Peña ◽  
Yuliya Lokha ◽  
Roberto Fernández-Lafuente
Keyword(s):  
Immobilized Enzyme ◽  
Biodiesel Production ◽  
Free Form ◽  
Enzyme Specificity ◽  
Agarose Beads ◽  
The Stability ◽  
Isoform B ◽  
First Time ◽  
Commercial Lipase

Eversa is an enzyme recently launched by Novozymes to be used in a free form as biocatalyst in biodiesel production. This paper shows for first time the immobilization of Eversa (a commercial lipase) on octyl and aminated agarose beads and the comparison of the enzyme properties to those of the most used lipase, the isoform B from Candida antarctica (CALB) immobilized on octyl agarose beads. Immobilization on octyl and aminated supports of Eversa has not had a significant effect on enzyme activity versus p-nitrophenyl butyrate (pNPB) under standard conditions (pH 7), but immobilization on octyl agarose beads greatly enhanced the stability of the enzyme under all studied conditions, much more than immobilization on aminated support. Octyl-Eversa was much more stable than octyl-CALB at pH 9, but it was less stable at pH 5. In the presence of 90% acetonitrile or dioxane, octyl-Eversa maintained the activity (even increased the activity) after 45 days of incubation in a similar way to octyl-CALB, but in 90% of methanol, results are much worse, and octyl-CALB became much more stable than Eversa. Coating with PEI has not a clear effect on octyl-Eversa stability, although it affected enzyme specificity and activity response to the changes in the pH. Eversa immobilized octyl supports was more active than CALB versus triacetin or pNPB, but much less active versus methyl mandelate esters. On the other hand, Eversa specificity and response to changes in the medium were greatly modulated by the immobilization protocol or by the coating of the immobilized enzyme with PEI. Thus, Eversa may be a promising biocatalyst for many processes different to the biodiesel production and its properties may be greatly improved following a suitable immobilization protocol, and in some cases is more stable and active than CALB.

scholarly journals Screening Suitability of Northern Hemisphere Algal Strains for Heterotrophic Cultivation and Fatty Acid Methyl Ester Production

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2107
Author(s):  
Jean Claude Nzayisenga ◽  
Calle Niemi ◽  
Lorenza Ferro ◽  
Andras Gorzsas ◽  
Francesco G. Gentili ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Municipal Wastewater ◽  
Fossil Fuel ◽  
Acid Methyl Ester ◽  
Biodiesel Production ◽  
Fossil Fuel Combustion ◽  
Heterotrophic Cultivation ◽  
Acid Methyl ◽  
Northern Areas ◽  
Ca 50

Rapid rises in atmospheric CO2 levels derived from fossil fuel combustion are imposing urgent needs for renewable substitutes. One environmentally friendly alternative is biodiesel produced from suitable microalgal fatty acids. Algal strains normally grow photoautotrophically, but this is problematic in Northern areas because of the light limitations for much of the year. Mixotrophic and particularly heterotrophic strains could be valuable, especially if they can be cultivated in municipal wastewater with contents of nutrients such as nitrogen and phosphorous that should be reduced before release into receiving water. Thus, the aim of this study was to screen for microalgal strains suitable for heterotrophic cultivation with a cheap carbon source (glycerol) for biodiesel production in Nordic, and other high-latitude, countries. One of the examined strains, a Desmodesmus sp. strain designated 2-6, accumulated biomass at similar rates in heterotrophic conditions with 40 mM glycerol as in autotrophic conditions. Furthermore, in heterotrophic conditions it produced more fatty acids, and ca. 50% more C18:1 fatty acids, as well as showing a significant decrease in C18:3 fatty acids, all of which are highly desirable features for biodiesel production.

scholarly journals Fatty acid methyl ester analysis of Aspergillus fumigatus isolated from fruit pulps for biodiesel production using GC-MS spectrometry

Bioengineered ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 408-415 ◽  
Author(s):  
Ferruh Asci ◽  
Busra Aydin ◽  
Gulderen Uysal Akkus ◽  
Arzu Unal ◽  
Sevim Feyza Erdogmus ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Methyl Ester ◽  
Aspergillus Fumigatus ◽  
Fatty Acid Methyl Ester ◽  
Acid Methyl Ester ◽  
Biodiesel Production ◽  
Acid Methyl
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