scholarly journals Ultrasound-Assisted Extraction of GAC Peel: An Optimization of Extraction Conditions for Recovering Carotenoids and Antioxidant Capacity

Processes ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 8 ◽  
Author(s):  
Hoang V. Chuyen ◽  
Paul D. Roach ◽  
John B. Golding ◽  
Sophie E. Parks ◽  
Minh H. Nguyen
Keyword(s):  
Antioxidant Capacity ◽  
Extraction Time ◽  
Carotenoid Content ◽  
Ultrasonic Power ◽  
Optimal Conditions ◽  
Total Carotenoid ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
The Impact

The peel of Gac fruit (Momordica cochinchinensis Spreng.), which is considered as waste of Gac processing, has been found to possess high levels of carotenoids and other antioxidants. This study aimed at determining the optimal conditions of an ultrasound-assisted extraction for recovering carotenoids and antioxidant capacity from Gac peel. A response surface methodology using the Box–Behnken design was employed to investigate the impact of extraction time, temperature and ultrasonic power on the recovery of total carotenoid and antioxidant capacity. The results showed that an extraction time of 76 min, temperature of 50 °C and ultrasonic power of 250 W were the optimal conditions for the extraction. The experimental carotenoid yield and antioxidant capacity obtained under the optimal extraction conditions were validated as 269 mg/100 g DW (dry weight) and 822 µM TE (Trolox equivalent)/100 g DW, respectively. These values were not significantly different from the values predicted by the models. The HPLC analysis for carotenoid composition showed that β-carotene, lycopene and lutein were the principal carotenoids of the extract, which constitute 86% of the total carotenoid content. Based on the obtained results, the ultrasound-assisted extraction using ethyl acetate under the above optimal conditions is suggested for the simultaneous recovery of carotenoids and antioxidant capacity from Gac peel.

Ultrasound-Assisted Extraction of Polysaccharides from Pleurotus eryngii (DC. ex Fr.) Quel

2012 ◽  
Vol 610-613 ◽  
pp. 3410-3415
Author(s):  
Yu Qin Tang ◽  
Yi Tao Zhao
Keyword(s):  
Orthogonal Experiment ◽  
Extraction Yield ◽  
Pleurotus Eryngii ◽  
Extraction Time ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Power Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

The purpose of this study was to select the optimum ultrasound-assisted extraction (UAE) conditions for the extraction of polysaccharides from Pleurotus eryngii (DC. ex Fr.) Que (P. eryngii ). The main factors that affect the extraction yield of polysaccharides such as solid:liquid ratio, ultrasonic power, extraction time and extraction temperature were studied individually. An orthogonal experiment was designed to optimize the extraction parameters. It was found that UAE method was a reliable, simple and effective method for fast extraction of polysaccharides from P. eryngii. The optimum UAE conditions were as followings: Solid:liquid ratio of 1:35, Ultrasonic power of 55 W, Extraction time of 30 min and extraction temperature of 45°C.

Optimization of Ultrasound-Assisted Extraction of Polysaccharides from the Chinese Medicinal Plant Rhodiola Sachalinensis A. BOR

2013 ◽  
Vol 448-453 ◽  
pp. 1085-1088
Author(s):  
Chuan Jun Zhang ◽  
Feng Lin Li
Keyword(s):  
Medicinal Plant ◽  
Extraction Time ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Rhodiola Sachalinensis ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Chinese Medicinal Plant

Ultrasound-assisted extraction (UAE) of polysaccharides from the Chinese medicinal plantRhodiola sachalinensisA. BOR (PRs) was studied. The effects of four factors (extraction time, ultrasonic power, liquid-solid ratio and extraction temperature) on the extraction yield of PRs were investigated by the single-factor test, and the optimum UAE conditions were investigated by the orthogonal test. The results demonstrated that the optimum UAE conditions for PRs were as followings: extraction time of 30 min, ultrasonic power of 85 W, liquid-solid ratio of 45 ml/g, and extraction temperature of 45°C. The present data might provide a theoretic base for full utilization ofRhodiola sachalinensisA. BOR.

scholarly journals Ultrasound Extraction Mediated Recovery of Nutrients and Antioxidant Bioactive Compounds from Phaeodactylum tricornutum Microalgae

2021 ◽  
Vol 11 (4) ◽  
pp. 1701
Author(s):  
Fadila Al Khawli ◽  
Francisco J. Martí-Quijal ◽  
Noelia Pallarés ◽  
Francisco J. Barba ◽  
Emilia Ferrer
Keyword(s):  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Influential Factor ◽  
Extraction Time ◽  
Added Value ◽  
Antioxidant Compounds ◽  
Ultrasound Assisted Extraction ◽  
Processing Technologies ◽  
Assisted Extraction ◽  
Ultrasound Assisted

In recent years, a growing interest has been shown in the use of microalgae due to their interesting nutritional and bioactive profiles. Green innovative processing technologies such as ultrasound-assisted extraction (UAE) avoid the use of toxic solvents and high temperatures, being a sustainable alternative in comparison with traditional extraction methods. The present study aims to evaluate the recovery of high added-value compounds from Phaedoactylum tricornutum assisted by ultrasound. To optimize the UAE of proteins, carbohydrates, pigments and antioxidant compounds, a response surface methodology was used. Carbohydrate extraction was positively affected by the temperature. However, for the extraction of carotenoids, the most influential factor was the extraction time. The total polyphenols were only significantly affected by the extraction time. Finally, the antioxidant capacity, measured by 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), was strongly modulated by the extraction time, while for the oxygen radical antioxidant capacity (ORAC) assay, the most important parameter was the temperature, followed by the extraction time. The optimal conditions for the maximum extraction of nutrients, bioactive compounds and antioxidant capacity were 30 min, 50 ºC and a pH of 8.5. Finally, it has been seen that with these conditions, the extraction of fucoxanthin is allowed, although no differences were found between an ultrasound-assisted extraction and a shaking extraction (control).

scholarly journals Development of a Natural Preservative from Chestnut Flowers: Ultrasound-Assisted Extraction Optimization and Functionality Assessment

Chemosensors ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 141
Author(s):  
Ikbel ben Alaya ◽  
Eliana Pereira ◽  
Maria Inês Dias ◽  
José Pinela ◽  
Ricardo C. Calhelha ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Biological Properties ◽  
Natural Antioxidant ◽  
In Vitro Assays ◽  
Ultrasonic Power ◽  
Optimal Conditions ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Anti Inflammatory

This study was carried out with the aim of optimizing the ultrasound-assisted extraction (UAE) of phenolic compounds from male chestnut flowers (C. sativa Mill) to develop a bioactive extract with potential to be used as a natural antioxidant preservative ingredient in the food industry. Time (t, 1–39 min), solvent concentration (S, 0–100%), and ultrasonic power (P, 5–500 W) were used as the independent variables for a 5-level experimental circumscribed central composite design (CCCD) coupled with response surface methodology (RSM) to optimize the extraction of phenolic compounds by UAE. Regarding the variables, the three showed a significant effect on the extraction of phenolic compounds. The content of phenolic compounds (including flavonoids and tannins) and the extraction yield (extract weight gravimetrically assessed) were the response criteria for the optimization. Based on the statistically validated predictive polynomial models, it was possible to reach a maximum content of phenolic compounds at the global optimal conditions of 24 ± 3 min, 259 ± 16 W, and 51 ± 7% ethanol. Additionally, pentagalloyl-glucoside and trigalloyl-hexahydroxydiphenoyl-glucoside were the major phenolic compounds identified. The optimized extract was then analyzed for their biological properties. The bioactive potential of the chestnut flower extract obtained under these optimized conditions was evaluated using in vitro assays for antioxidant, anti-inflammatory, and antimicrobial activity, as well as cytotoxicity and hepatotoxicity tests. The results revealed that the enriched extract has antioxidant, antitumoral, and anti-inflammatory activities without toxicity issues. Overall, this study allowed to define the optimal conditions for the extraction of phenolic compounds from chestnuts male flowers by UAE, to obtain an enriched extract with biological properties that could be further used as a natural antioxidant ingredient with applications on functional foods.

scholarly journals Ultrasound-assisted extraction of protein from Bombay locusts and its impact on functional and antioxidative properties

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Passakorn Kingwascharapong ◽  
Manat Chaijan ◽  
Supatra Karnjanapratum
Keyword(s):  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Surface Hydrophobicity ◽  
Molecular Characteristics ◽  
Ultrasound Assisted Extraction ◽  
Antioxidative Properties ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Nonessential Amino Acids ◽  
The Impact

AbstractImpact of ultrasound-assisted process (UAP) on yield, functional properties, antioxidant properties and molecular characteristics of protein extracted from Bombay locusts (BL) (Patanga succinta L.) was studied. Different conditions of UAP were implemented for different amplitudes (40–60%) and times (10–30 min) during aqueous extraction. Notably, UAP could enhance yield and protein recovery, compared with those from typical process (TP) (continuously stirred at 100 rpm at room temperature for 1 h). UAP conditions used governed the change of surface hydrophobicity and free α-amino content of BL. UAP could improve solubility of BL, especially at pH levels higher than 2. UAP had no significant (p > 0.05) detrimental effects on foaming capacity and stability of BL. Nevertheless, UAP, particularly at 50–60% amplitudes, affected the emulsion activity and stability of BL. UAP provided BL with high radical scavenging activities and good electron donating ability, especially that from 60% amplitude for 20 min (UAP-60/20). UAP-60/20 showed the impact on change of isoelectric point and molecular characteristic monitored by Fourier transform infrared (FTIR) of BL, compared to those from TP. In addition, BL was also an excellent source of both essential and nonessential amino acids. Therefore, UAP potentially enhanced BL extraction efficiency, resulting the BL with good functional and antioxidative properties.

scholarly journals Skin-Whitening and Anti-Wrinkle Effects of Bioactive Compounds Isolated from Peanut Shell Using Ultrasound-Assisted Extraction

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1231
Author(s):  
Da Hye Gam ◽  
Ji Woo Hong ◽  
Jun Hee Kim ◽  
Jin Woo Kim
Keyword(s):  
Ethanol Concentration ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Peanut Shell ◽  
Ultrasound Assisted Extraction ◽  
Skin Whitening ◽  
Activity Inhibition ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Experimental Values

Response surface methodology was employed to optimize the ultrasound-assisted extraction (UAE) conditions for simultaneous optimization of dependent variables, including DPPH radical scavenging activity (RSA), tyrosinase activity inhibition (TAI), and collagenase activity inhibition (CAI) of peanut shell extracts. The effects of the main variables including extraction time (5.0~55.0 min, X1), extraction temperature (26.0~94.0 °C, X2), and ethanol concentration (0.0%~99.5%, X3) were optimized. Based on experimental values from each condition, quadratic regression models were derived for the prediction of optimum conditions. The coefficient of determination (R2) of the independent variable was in the range of 0.89~0.96, which demonstrates that the regression model is suitable for the prediction. In predicting optimal UAE conditions based on the superimposing method, extraction time of 31.2 min, extraction temperature of 36.6 °C, and ethanol concentration of 93.2% were identified. Under these conditions, RSA of 74.9%, TAI of 50.6%, and CAI of 86.8% were predicted, showing good agreement with the experimental values. A reverse transcription polymerase chain reaction showed that peanut shell extract decreased mRNA levels of tyrosinase-related protein-1 and matrix metalloproteinase-3 genes in B16-F0 cell. Therefore, we identified the skin-whitening and anti-wrinkle effects of peanut shell extracts at protein as well as gene expression levels, and the results show that peanut shell is an effective cosmetic material for skin-whitening and anti-wrinkle effects. Based on this study, peanut shell, which was considered a byproduct, can be used for the development of healthy foods, medicines, and cosmetics.

scholarly journals Ultrasound-Assisted Extraction of Total Flavonoids from Corn Silk and Their Antioxidant Activity

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Ling-Li Zheng ◽  
Guan Wen ◽  
Min-Yong Yuan ◽  
Feng Gao
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Antioxidant Activities ◽  
Total Flavonoids ◽  
Ultrasonic Power ◽  
Ultrasound Assisted Extraction ◽  
Antioxidant Power ◽  
Corn Silk ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Object. Ultrasound-assisted extraction of total flavonoids from corn silk and their antioxidant activities were studied.Methods. Response surface methodology was adopted to optimize the extraction conditions and antioxidant activities of the extracted total flavonoids were detected through ferric reducing antioxidant power (FRAP) assay.Results. Through a three-level, three-variable Box-Behnken design of response surface methodology (RSM) adopting yield as response, the optimal conditions were determined as follows: ultrasonic power 500 W, extraction time 20 min, material solvent ratio 1 : 20, and ethanol concentration 30%. Under the optimum conditions, the extraction yield of total flavonoids was 1.13%. FRAP value of total flavonoids extracted from corn silk was 467.59 μmol/L.Conclusion. The total flavonoids of corn silk could be developed as food natural antioxidant reagents.

Protein Extraction from Spirulina platensis by Using Ultrasound Assisted Extraction: Effect of Solvent Types and Extraction Time

2021 ◽  
Vol 872 ◽  
pp. 33-37
Author(s):  
Kong Sela ◽  
Wiratni Budhijanto ◽  
Arief Budiman
Keyword(s):  
Spirulina Platensis ◽  
Protein Extraction ◽  
Solvent Mixture ◽  
Industrial Applications ◽  
Extraction Time ◽  
Protein Recovery ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Solvent Volume

Protein is a substantial nutrition that essentially required by human. Spirulina platensis (Spp), well known as protein source could be a significant source to be used for many industrial applications. This study was investigated the effectiveness of ultrasound assisted extraction (UAE) method for protein extraction from Spp at various composition of solvent mixture and extraction time. Ethanol and mixture of methanol-ethanol were used as solvent. Extraction was conducted by varying ratios of solvent to biomass at 10:1, 12.5:1, and 15:1 (v/w), and extraction time (20, 35, and 50 min). Optimum protein recovery from dry Spp was 42.55 ± 0.43% obtained by using 20 ml of the mixture of methanol and ethanol at 50 min of extraction time. This study also conducted that mixture of methanol and ethanol was a better solvent on improving the ultrasound assisted extraction, as indicated by high protein recovery with less amount of solvent volume used.

scholarly journals Optimization of Ultrasound-Assisted Extraction of Antioxidant Polyphenols from the Seed Coats of Red Sword Bean (Canavalia gladiate (Jacq.) DC.)

Antioxidants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 200 ◽  
Author(s):  
Yue Zhou ◽  
Xiao-Yu Xu ◽  
Ren-You Gan ◽  
Jie Zheng ◽  
Ya Li ◽  
...  
Keyword(s):  
Extraction Method ◽  
Natural Antioxidants ◽  
Extraction Time ◽  
Antioxidant Compounds ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Seed Coats

The seed coat of red sword bean (Canavalia gladiata (Jacq.) DC.) is rich in antioxidant polyphenols. It is often discarded as a byproduct with the consumption of red sword bean, since it is very thick and not consumed by people. The aim of this study was to develop an ultrasound-assisted extraction method to extract natural antioxidants from the seed coats. The extraction process was optimized by using response surface methodology. After the single-factor experiments, three key factors, including ethanol concentration, liquid/solid ratio, and extraction time, were selected and their interactions were studied using a central composite design. The optimal extraction condition was 60.2% hydroethanol, a liquid/solid ratio of 29.3 mL/g, an extraction time of 18.4 min, an extraction temperature of 50 °C, and ultrasound power of 400 W. Under the optimal conditions, antioxidant activity of the extract was 755.98 ± 10.23 μmol Trolox/g dry weight (DW), much higher than that from maceration (558.77 ± 14.42 μmol Trolox/g DW) or Soxhlet extraction (479.81 ± 12.75 μmol Trolox/g DW). In addition, the main antioxidant compounds in the extract were identified and quantified by high-performance liquid chromatography–diode array detection–tandem mass spectrometry (HPLC–DAD–MS/MS). The concentrations of digalloyl hexoside, methyl gallate, gallic acid, trigalloyl hexoside, and digallic acid were 15.30 ± 0.98, 8.85 ± 0.51, 8.76 ± 0.36, 4.27 ± 0.21, and 2.89 ± 0.13 mg/g DW. This study provides an efficient and green extraction method for the extraction of natural antioxidants from the bean coat of red sword bean. The extract of antioxidants might be added into functional foods or nutraceuticals with potential beneficial functions.

scholarly journals Effects of Microwave-Assisted Extraction Conditions on Antioxidant Capacity of Sweet Tea (Lithocarpus polystachyus Rehd.)

Antioxidants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 678
Author(s):  
Ao Shang ◽  
Min Luo ◽  
Ren-You Gan ◽  
Xiao-Yu Xu ◽  
Yu Xia ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Microwave Power ◽  
Ethanol Concentration ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Microwave Assisted Extraction ◽  
Optimal Conditions ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Sample Ratio

In this study, the effects of microwave-assisted extraction conditions on antioxidant capacity of sweet tea (Lithocarpus polystachyus Rehd.) were studied and the antioxidants in the extract were identified. The influences of ethanol concentration, solvent-to-sample ratio, microwave power, extraction temperature and extraction time on Trolox equivalent antioxidant capacity (TEAC) value, ferric reducing antioxidant power (FRAP) value and total phenolic content (TPC) were investigated by single-factor experiments. The response surface methodology (RSM) was used to study the interaction of three parameters which had significant influences on antioxidant capacity including ethanol concentration, solvent-to-sample ratio and extraction time. The optimal conditions for the extraction of antioxidants from sweet tea were found as follows—ethanol concentration of 58.43% (v/v), solvent-to-sample ratio of 35.39:1 mL/g, extraction time of 25.26 min, extraction temperature of 50 ℃ and microwave power of 600 W. The FRAP, TEAC and TPC values of the extract under the optimal conditions were 381.29 ± 4.42 μM Fe(II)/g dry weight (DW), 613.11 ± 9.32 μM Trolox/g DW and 135.94 ± 0.52 mg gallic acid equivalent (GAE)/g DW, respectively. In addition, the major antioxidant components in the extract were detected by high-performance liquid chromatography with diode array detection (HPLC-DAD), including phlorizin, phloretin and trilobatin. The crude extract could be used as food additives or developed into functional food for the prevention and management of oxidative stress-related diseases.

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