scholarly journals Techno-Economic Implications of Fed-Batch Enzymatic Hydrolysis

Processes ◽  
2019 ◽  
Vol 7 (11) ◽  
pp. 847 ◽  
Author(s):  
Ellen Argo ◽  
Deepak R. Keshwani
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ethanol Production ◽  
Process Simulation ◽  
Cellulosic Ethanol ◽  
Production Cost ◽  
Simulation Software ◽  
Labor Costs ◽  
Fed Batch ◽  
Capital Costs ◽  
Plant Capacity

Fed-batch enzymatic hydrolysis has the potential to improve the overall process of converting cellulosic biomass into ethanol. This paper utilizes a process simulation approach to identify and quantify techno-economic differences between batch and fed-batch enzymatic hydrolysis in cellulosic ethanol production. The entire process of converting corn stover into ethanol was simulated using SuperPro Designer simulation software. The analysis was conducted for a plant capacity of 2000 metric tons of dry biomass per day. A literature review was used to identify baseline parameters for the process. The sensitivity of the ethanol production cost to changes in sugar conversion efficiency, plant capacity, biomass cost, power cost, labor cost, and enzyme cost was evaluated using the process simulation. For the base scenario, the ethanol unit production cost was approximately $0.10/gallon lower for fed-batch hydrolysis. The greatest differences were seen in facilities costs, labor costs, and capital costs. Using a fed-batch operation decreased facilities costs by 41%, labor costs by 21%, and capital costs by 15%. The sensitivity analysis found that cost of biomass had the greatest effect on ethanol production cost, and in general, the results support the proposition that fed-batch enzymatic hydrolysis does improve the techno-economics of cellulosic ethanol production.

scholarly journals A novel recyclable furoic acid-assisted pretreatment for sugarcane bagasse biorefinery in co-production of xylooligosaccharides and glucose

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Lin Dai ◽  
Tian Huang ◽  
Kankan Jiang ◽  
Xin Zhou ◽  
Yong Xu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Production Cost ◽  
Solid Loading ◽  
Fed Batch ◽  
Acid Pretreatment ◽  
Furoic Acid ◽  
Cooling Crystallization ◽  
Mineral Acids ◽  
Hydrolysis Of

Abstract Background Pretreatment is the key step for utilizing lignocellulosic biomass, which can extract cellulose from lignin and disrupt its recalcitrant crystalline structure to allow much more effective enzymatic hydrolysis; and organic acids pretreatment with dual benefic for generating xylooligosaccharides and boosting enzymatic hydrolysis has been widely used in adding values to lignocellulose materials. In this work, furoic acid, a novel recyclable organic acid as catalyst, was employed to pretreat sugarcane bagasse to recover the xylooligosaccharides fraction from hemicellulose and boost the subsequent cellulose saccharification. Results The FA-assisted hydrolysis of sugarcane bagasse using 3% furoic acid at 170 °C for 15 min resulted in the highest xylooligosaccharides yield of 45.6%; subsequently, 83.1 g/L of glucose was harvested by a fed-batch operation with a solid loading of 15%. Overall, a total of 120 g of xylooligosaccharides and 335 g glucose could be collected from 1000 g sugarcane bagasse starting from the furoic acid pretreatment. Furthermore, furoic acid can be easily recovered by cooling crystallization. Conclusion This work put forward a novel furoic acid pretreatment method to convert sugarcane bagasse into xylooligosaccharides and glucose, which provides a strategy that the sugar and nutraceutical industries can be used to reduce the production cost. The developed process showed that the yields of xylooligosaccharides and byproducts were controllable by shortening the reaction time; meanwhile, the recyclability of furoic acid also can potentially reduce the pretreatment cost and potentially replace the traditional mineral acids pretreatment.

scholarly journals A novel recyclable furoic acid-assisted pretreatment for sugarcane bagasse biorefinery in co-production of xylooligosaccharides and glucose

2020 ◽  
Author(s):  
Lin Dai ◽  
Tian Huang ◽  
Kankan Jiang ◽  
Xin Zhou ◽  
Yong Xu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Production Cost ◽  
Solid Loading ◽  
Fed Batch ◽  
Acid Pretreatment ◽  
Furoic Acid ◽  
Cooling Crystallization ◽  
Mineral Acids ◽  
Hydrolysis Of

Abstract Background: Pretreatment is the key step for utilizing lignocellulosic biomass, which can extract cellulose from lignin and disrupt its recalcitrant crystalline structure to allow much more effective enzymatic hydrolysis; and organic acids pretreatment with dual benefic for generating xylooligosaccharides and boosting enzymatic hydrolysis has been widely used in adding values to lignocellulose materials. In this work, furoic acid, a novel recyclable organic acid as catalyst, was employed to pretreat sugarcane bagasse to recover the xylooligosaccharides fraction from hemicellulose and boost the subsequent cellulose saccharification. Results: The FA-assisted hydrolysis of sugarcane bagasse using 3% furoic acid at 170 oC for 15 min resulted in the highest xylooligosaccharides yield of 45.6%; subsequently, 83.1 g/L of glucose was harvested by a fed-batch operation with a solid loading of 15%. Overall, a total of 120 g of xylooligosaccharides and 335 g glucose could be collected from 1000 g sugarcane bagasse starting from the furoic acid pretreatment. Furthermore, furoic acid can be easily recovered by cooling crystallization.Conclusion: This work put forward a novel furoic acid pretreatment method to convert sugarcane bagasse into xylooligosaccharides and glucose, which provides a strategy that the sugar and nutraceutical industries can be used to reduce the production cost. The developed process showed that the yields of xylooligosaccharides and byproducts were controllable by shortening the reaction time; meanwhile, the recyclability of furoic acid also can potentially reduce the pretreatment cost and potentially replace the traditional mineral acids pretreatment.

scholarly journals Increase in ethanol production from sugarcane bagasse based on combined pretreatments and fed-batch enzymatic hydrolysis

2013 ◽  
Vol 128 ◽  
pp. 448-453 ◽  
Author(s):  
Maria Carolina de Albuquerque Wanderley ◽  
Carlos Martín ◽  
George Jackson de Moraes Rocha ◽  
Ester Ribeiro Gouveia
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ethanol Production ◽  
Sugarcane Bagasse ◽  
Fed Batch

Strategies for the Cellulosic Ethanol Production by Using High-Concentration Poly(ethylene glycol) in the Pretreatment, Enzymatic Hydrolysis, and Fermentation Steps

2021 ◽  
Author(s):  
Cleitiane da Costa Nogueira ◽  
Carlos Eduardo de Araújo Padilha ◽  
Alexande de Araújo Gilherme ◽  
Domingos Fabiano Santana de Souza ◽  
Jackson Araújo de Oliveira ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ethylene Glycol ◽  
Ethanol Production ◽  
Cellulosic Ethanol ◽  
Poly Ethylene Glycol ◽  
High Concentration ◽  
Poly Ethylene

Comparison of Low-Temperature Alkali/Urea Pretreatments for Ethanol Production from Wheat Straw

2021 ◽  
Vol 15 (3) ◽  
pp. 399-407
Author(s):  
Zahoor ◽  
Wen Wang ◽  
Xuesong Tan ◽  
Qiang Yu ◽  
Yongming Sun ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Low Temperature ◽  
Ethanol Production ◽  
Wheat Straw ◽  
Response Surface ◽  
Cellulosic Ethanol ◽  
Enzymatic Saccharification ◽  
Hydrolysis Of ◽  
Bioethanol Yield

NaOH/urea (NU) pretreatment at lower than 0 °C has been frequently applied for improving bio-conversion of lignocellulose, but the wastewater generated from the pretreatment process is hard to dispose. KOH/urea (KU) pretreatment for enhancing bioconversion of lignocellulose has recently attracted researchers’ attention due to the recycling of wastewater for facilitating crops’ growth. This study compared the effects of NU and KU pretreatments at cold conditions on the enzymatic hydrolysis and bioethanol yield from wheat straw (WS). By using response surface methodology an optimal pretreatment with an equal ratio of alkali/urea (4% w/v) at −20 °C for 3 h was established. The enzymatic hydrolysis of KU-treated WS was 81.17%, which was similar to that of NU-treated WS (83.72%) under the same condition. It means that KU pretreatment has equal ability to NU pretreatment to improve enzymatic saccharification of lignocellulose. KU pretreatment has the promising potential to replace NU pretreatment for facilitating bioconversion of lignocellulose in cold conditions due to the clean way to recycle its wastewater as fertilizer for crop growth. Hence, KU pretreatment combined with enzymatic hydrolysis and fermentation could be a promising green way to cellulosic ethanol production with zero waste emission.

Use of ionic liquids for improvement of cellulosic ethanol production

BioResources ◽  
2010 ◽  
Vol 6 (1) ◽  
pp. 1-2
Author(s):  
Qijun Wang ◽  
Yuanxin Wu ◽  
Shengdong Zhu
Keyword(s):  
Ionic Liquids ◽  
Enzymatic Hydrolysis ◽  
Ethanol Production ◽  
Lignocellulosic Biomass ◽  
Cellulosic Ethanol ◽  
Heterogeneous Reaction ◽  
Reaction System ◽  
The Other ◽  
Fermentable Sugars ◽  
Hydrolysis Process

Cellulosic ethanol production has drawn much attention in recent years. However, there remain significant technical challenges before such production can be considered as economically feasible at an industrial scale. Among them, the efficient conversion of carbohydrates in lignocellulosic biomass into fermentable sugars is one of the most challenging technical difficulties in cellulosic ethanol production. Use of ionic liquids has opened new avenues to solve this problem by two different pathways. One is pretreatment of lignocellulosic biomass using ionic liquids to increase its enzymatic hydrolysis efficiency. The other is to transform the hydrolysis process of lignocellulosic biomass from a heterogeneous reaction system to a homogeneous one by dissolving it into ionic liquids, thus improving its hydrolysis efficiency.

Potential Impact on Activated Sludge Treatment from the Implementation of Cellulosic Ethanol Production at a Pulp and Paper Mill

2009 ◽  
Vol 4 (1) ◽  
Author(s):  
Jean-Martin Brault ◽  
Edouard Kouakou ◽  
Virginie Chambost ◽  
Paul Stuart
Keyword(s):  
Ethanol Production ◽  
Production Rate ◽  
Cellulosic Ethanol ◽  
Treatment Plant ◽  
Oxygen Demand ◽  
Paper Mill ◽  
Operating Costs ◽  
Spare Capacity ◽  
Capital Costs ◽  
The Impact

The potential impacts of additional flow and organic loads resulting from the production of cellulosic ethanol on an existing integrated newsprint mill were simulated in this paper. It was found that depending on the ethanol production rate and the existing spare capacity for additional biochemical oxygen demand (BOD), treatment plant modifications may be required. In terms of operating costs, it was found that nutrients use could increase by 50% to 150%, while aeration flow could increase by 5% to 140% depending on the desired level of dissolved oxygen in the aeration basin. Significant increases in polymer use for mixed sludge dewatering could result due to additional biosolids production. Additional capital costs for air blowers could also be necessary unless the mill has existing spare capacity. It was also found that ethanol recovery efficiency and production rate had little impact on BOD removal up to a certain ethanol production level, and that the impact on operating costs decreased with increasing recovery.

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