scholarly journals Ultrasound-Assisted Phytochemical Extraction Condition Optimization Using Response Surface Methodology from Perlette Grapes (Vitis vinifera)

Processes ◽  
2019 ◽  
Vol 7 (10) ◽  
pp. 749 ◽  
Author(s):  
Muhammad Kaleem ◽  
Asif Ahmad ◽  
Rai Muhammad Amir ◽  
Ghazala Kaukab Raja
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Acetic Acid ◽  
Vitis Vinifera ◽  
Central Composite Design ◽  
Flavonoid Content ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Central Composite

In the current study, bioactive compounds of Vitis vinifera (Perlette) were extracted using an ultrasound-assisted extraction technique. The central composite design of response surface methodology (RSM) was used to determine the effect of time, temperature, and concentration of acetic acid on response variables that include extract yield, total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity of Vitis vinifera extracts. The results of the central composite design of RSM revealed that the quadratic polynomial model is best fitted to experimental results, with all the responses having a regression coefficient greater than 0.9. Optimized extraction levels include 26.5 min, an extraction temperature of 59 °C, and an acetic acid concentration of 62.9% with good extraction yield results of 34.95 g/100 g dry weight (DW) of grapes, TPC 34.38 mg gallic acid equivalent per gram (GAE/g) DW, flavonoid content 10.21 mg quercetin equivalents per gram (QEQ/g) DW, and antioxidant activity of 9.11 mg/100 mL ascorbic acid equivalent. The present study showed that acetic acid can be effectively used for the ultrasound-assisted extraction of polyphenols, flavonoids, and antioxidants of Vitis vinifera. These optimized conditions can be used for the extraction of bioactive compounds that can be for the development of nutraceutical products.

scholarly journals OPTIMASI KONDISI EKSTRAKSI ULTRASONIKASI PADA VITAMIN C BUAH DELIMA (Punica granatum L.) MENGGUNAKAN RESPON PERMUKAAN

2021 ◽  
Vol 9 (2) ◽  
pp. 143-158
Author(s):  
S. Rosalinda ◽  
Hilda Ayu Aulia ◽  
Asri Widyasanti ◽  
Efri Mardawati
Keyword(s):  
Response Surface Methodology ◽  
Vitamin C ◽  
Central Composite Design ◽  
Response Surface ◽  
Punica Granatum ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Punica Granatum L ◽  
Central Composite

Buah Delima (Punica granatum L.) merupakan tanaman yang memiliki banyak manfaat, diantaranya memiliki kandungan antioksidan. Kandungan antioksidan dalam buah delima yang utama adalah vitamin C. Vitamin C sebagai antioksidan dibutuhkan dalam sistem imun untuk menjaga kekebalan tubuh dan juga dapat menjaga elastisitas kulit. Kandungan vitamin C dari buah delima dapat diperoleh dengan cara ekstraksi. Vitamin C tidak tahan pada suhu yang tinggi, sehingga dibutuhkan teknologi ekstraksi yang tepat. Ultrasound Assisted Extraction (UAE) merupakan ekstraksi ultrasonikasi yang dipilih karena tidak menggunakan suhu yang tinggi dan waktu ekstraksi lebih singkat. Penelitian ini bertujuan menentukan kondisi UAE optimum yang meliputi kombinasi 3 variabel, yaitu amplitudo, waktu, dan jumlah pelarut yang menghasilkan vitamin C optimum dari ekstrak daging buah delima. Tipe alat UAE yang digunakan pada penelitian ini adalah tipe Qsonica – Q500 (500 W, 20 kHz). Metode penelitian adalah eksperimental laboratorium menggunakan RSM (Response Surface Methodology) tipe CCD (Central Composite Design) dengan amplitudo minimum 40% dan maksimum 50%; waktu minimum 20 menit dan maksimum 25 menit; dan jumlah pelarut minimum 150 ml dan maksimum 225 ml. Hasil penelitian menyimpulkan bahwa banyaknya jumlah pelarut memberikan pengaruh yang nyata, sedangkan amplitudo dan lama waktu ekstraksi tidak berpengaruh nyata. Model signifikan pada nilai P < 0,05 dengan kondisi UAE optimum pada kombinasi perlakuan amplitudo 50%, waktu 25 menit, dan jumlah pelarut 150 ml yang menghasilkan nilai kadar vitamin C optimum sebesar 47,79 mg/100 gram.

scholarly journals Simultaneous Optimization of Ultrasound-Assisted Extraction for Flavonoids and Antioxidant Activity of Angelica keiskei Using Response Surface Methodology (RSM)

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3461 ◽  
Author(s):  
Lei Zhang ◽  
Yuhuan Jiang ◽  
Xuening Pang ◽  
Puyue Hua ◽  
Xiang Gao ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Biological Activities ◽  
Ultrasound Assisted Extraction ◽  
Antioxidant Power ◽  
Assisted Extraction ◽  
Ultrasonic Time ◽  
Angelica Keiskei ◽  
Ultrasound Assisted

Angelica keiskei Koidzumi (A. keiskei), as a Japanese edible herbal plant, enjoys a variety of biological activities due to the presence of numerous active compounds, especially flavonoids. This study aims for the optimization of ultrasound-assisted extraction (UAE) for flavonoids in A. keiskei and their antioxidant activity by using the response surface methodology (RSM). Single-factor experiments and a four-factor three-level Box–Behnken design (BBD) were performed to explore the effects of the following parameters on flavonoid extraction and antioxidant activity evaluation: ultrasonic temperature (X1), ultrasonic time (X2), ethanol concentration (X3) and liquid–solid ratio (X4). The optimum conditions of the combination of total flavonoid content (TFC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity (DPPH-RSC) and ferric-reducing antioxidant power (FRAP) were as follows: X1 = 80 °C, X2 = 4 min, X3 = 78%, X4 = 35 mL/g, respectively. The experimental results provide a theoretical basis for the extensive utilization of A. keiskei and flavonoids extraction from A. keiskei as a potential source of antioxidants.

scholarly journals Ultrasound-Assisted Extraction using Response Surface Methodology for Extracting Flavonoids from Padina australis

2021 ◽  
Vol 50 (5) ◽  
pp. 1321-1328
Author(s):  
Bina Lohita Sari ◽  
Tri Saptari Haryani ◽  
Triastinurmiatiningsih Triastinurmiatiningsih ◽  
Dinda Rizki Amalia
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Total Flavonoid ◽  
Process Time ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Ultrasound Assisted Extraction ◽  
Brown Macroalgae ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Seaweed or sea macroalgae are rich in potential compounds which can be used for the treatment of disease. Padina australis is one of the important brown macroalgae classes (Phaeophyes). One of the bioactive compounds of P. australis is a phenolic compound and its derivatives (flavonoid). In this research, P. australis was collected from Bayah Beach, Banten, Indonesia. For the extraction flavonoids from P. australis, ultrasound-assisted extraction (UAE) was employed. In this study, a three-level Box-Behnken design (BBD) and the response surface methodology (RSM) were employed to obtain the optimal combination of extraction conditions. The effects of several independent variables including temperature (30, 50, 70 ℃), extraction time (20, 40, 60 min) and ethanol concentration (30, 50, 70%) were investigated. The result showed that RSM was an accurate and reliable method in predicting the total flavonoid content with R2 value of 0.9935. The optimal UAE conditions for the highest yield of total flavonoid content were 49.70 ℃ in temperature, process time under 44.03 min, and 47.80% ethanol with 0.2162% total flavonoid content. Under the above conditions, the experimental value of total flavonoid content was 0.2144+ 0.0035%. The predicted and experimental values for total flavonoid from brown algae P. australis were not significant differences, it indicating that the developed models are accurate. Therefore, UAE using RSM is effective for the extraction of flavonoid from P. australis.

Sign in / Sign up

Export Citation Format

Share Document