scholarly journals Separation of Protein-Binding Anthraquinones from Semen Cassiae Using Two-Stage Foam Fractionation

Processes ◽  
2019 ◽  
Vol 7 (7) ◽  
pp. 463 ◽  
Author(s):  
Linlin Ding ◽  
Yanyan Wang ◽  
Chuanjun Yue ◽  
Zeying Wu ◽  
Ye Sun ◽  
...  
Keyword(s):  
Protein Binding ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Process Intensification ◽  
Enrichment Ratio ◽  
Foam Fractionation ◽  
Feed Concentration ◽  
Two Stage ◽  
Total Recovery ◽  
Medicinal Value

Anthraquinones are compounds of high medicinal value in many plants. Based on their good protein binding affinity, foam fractionation was attempted to separate them using proteins in the aqueous extract of Semen Cassiae as collectors. Firstly, the interaction between anthraquinones and Semen Cassiae proteins has been analyzed by the Stem–Volmer equation with physcion as a standard. The results show that physcion had good interaction with the proteins via hydrophobic forces. More importantly, the proteins effectively assisted the foam fractionation of several anthraquinones including aurantio-obtusifolin, aloe-emodin, rhein, emodin, chrysophanol, and physcion. On this basis, a two-stage foam fractionation technology was developed for process intensification using a foam fractionation with vertical sieve trays (VSTs). VSTs, initial feed concentration of total anthraquinones, temperature, volumetric air flow rate and pore diameter of gas distributor had significant effects on enrichment ratio and recovery yield of anthraquinones. Under suitable conditions, the enrichment ratio of total anthraquinones reached 47.0 ± 4.5 with a concentration of 939 ± 94 mg/L in the foamate while their total recovery percentage reached more than 47.7%. In addition, foam fractionation also increased the purity and hydroxyl radical scavenging activity of total anthraquinones. The results had significant implications for the separation of anthraquinones from Semen Cassiae.

FREE RADICAL SCAVENGING ACTIVITY OF ACACIA NILOTICA PODS

INDIAN DRUGS ◽  
2014 ◽  
Vol 51 (07) ◽  
pp. 14-22
Author(s):  
T. A Khan ◽  
◽  
A. Gohel
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Folk Medicine ◽  
Radical Scavenging ◽  
Acacia Nilotica ◽  
Free Radical Scavengers ◽  
Radical Scavengers ◽  
Radical Production ◽  
Medicinal Value

Biomolecules can be oxidized by free radicals. This oxidative damage has an important etiological role in aging and the development of diseases like cancer, atherosclerosis, and other inflammatory disorders. Synthetic antioxidants, such as butylated hydroxyl anisole, are good free radical scavengers; however, they can be carcinogenic. Therefore, there is an increasing interest in searching for antioxidants of natural origin. Acacia nilotica has a versatile medicinal value as folk medicine. The present study aims at screening the methanol and aqueous extracts of the pods of Acacia nilotica for antioxidant activity. The effect of these extracts on inhibition of 1, 1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide radicals and of lipid peroxidation was studied. The results indicated that both the extracts exhibited a significant reduction in the DPPH and NO radical production along with a significant reduction in lipid peroxidation. The results indicate the pods of Acacia nilotica to be a cheap and easily available source of antioxidants

scholarly journals HPLC identification of Rutin (3, 3′, 4′, 5, 7-pentahydroxyflavone-3-rhamnoglucoside) from Costus pictus leaves extract and evaluation of its antioxidant activity

2020 ◽  
Vol 6 (2) ◽  
pp. 334-339
Author(s):  
S Vaidehi ◽  
G Sriram Prasath ◽  
J Uma Maheswari ◽  
N Rajasekar
Keyword(s):  
Ethyl Acetate Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Food Supplement ◽  
Pharmaceutical Products ◽  
Hplc Method ◽  
Free Radical Scavenging ◽  
Natural Food ◽  
Scavenging Activity ◽  
Medicinal Value

Plants synthesise many secondary metabolites which are of great medicinal value. Costus pictus also known as Insulin plant is considered as valuable medicinal plant in preparation of many commercial pharmaceutical products. The aim of the present study is to identify one such secondary metabolite flavonoid (Rutin) in the leaves of Costus pictus by HPLC method and also to evaluate the antioxidant potential of Ethyl acetate extract of Costus using DPPH and NO scavenging assay. The Extracted materi al was identified as Rutin by comparing with Standard Rutin. Both the samples showed similar retention time of 2.8 minutes. The extract showed maximum DPPH free radical scavenging activity with increase in concentration and IC50 of Nitric acid scavenging activity was found to be more for the Extract than the standard ascorbic acid and Rutin. From this study it is concluded that Costus pictus leaves extract can be used as natural food supplement. Asian J. Med. Biol. Res. June 2020, 6(2): 334-339

scholarly journals Wet milling of buckwheat cultivars and some quality properties of the fractions

2021 ◽  
pp. 108201322110113
Author(s):  
Ayşenur Arslan ◽  
Claudia Monika Haros ◽  
Erkan Yalçın ◽  
Ahmet Güneş
Keyword(s):  
Phenolic Compound ◽  
Dietary Fibre ◽  
Radical Scavenging Activity ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Radical Scavenging ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Wet Milling ◽  
Total Recovery

The hulled buckwheat cultivars ( Aktaş cv. and Güneş cv.) were wet-milled, and then some chemical, yields, colour, functional properties, phenolic compound, antioxidant activity, and pasting, thermal and retrogradation properties of starches were investigated and compared with the wholegrain buckwheat flour (with hull) and buckwheat groat flour (without hull) of the same cultivars. Sodium dodecyl sulphate polyacrylamide gel electrophoresis patterns of flours and protein fractions were examined under reducing and non-reducing conditions. The hull, germ+dietary fibre, protein and starch fractions were collected. The total recovery for Aktaş cv. and Güneş cv. cultivars were 98.1% and 96.1%; total starch yields were 51.6% and 49.7%; pasting temperatures of the starches were found as 83.7 and 85.7°C; and final viscosities of starches were determined as 3.5 and 3.4 Pa·s, respectively. The resistant starch contents of starch fractions of Aktaş cv. ve Güneş cv. were found as 3.28% and 3.62%, respectively. The highest total phenolic compound contents were detected with dimethyl sulphoxide extraction in the germ+dietary fibre fractions. The highest 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and trolox equivalent antioxidant capacity were found in the hull fraction (as 81.7%) and germ+dietary fibre fraction (as 11.8 mmol/kg) of Aktaş cv. cultivar.

scholarly journals Extraction of Polyphenolics from Apple Juice by Foam Fractionation

2006 ◽  
Vol 2 (2) ◽  
Author(s):  
Zaid Saleh ◽  
Roger Stanley ◽  
Mayank Nigam
Keyword(s):  
Flow Rate ◽  
Bubble Size ◽  
Low Flow ◽  
Enrichment Ratio ◽  
Foam Fractionation ◽  
Total Phenolic ◽  
Separation Performance ◽  
Feed Concentration ◽  
Solution Ph ◽  
Food Ingredients

This research was undertaken to test the feasibility of using the foam technology to separate polyphenols from fruit juices for use as functional food ingredients. The separation performance, expressed as enrichment ratio, selectivity and percentage recovery, was determined as a function of operating variables, namely air or N2 flow rate, initial feed concentration, bubble size, solution pH and the presence of alcohol to modify the surface tension. Measurements were made of the average bubble size and gas hold-up volume to calculate interfacial area. The bulk phase concentrations of the polyphenolics in the feed and foam fractions were analysed for total phenolic content by Folin assay and phenolic composition by reverse phase HPLC. Enrichment factors of up to 6 were found under optimum conditions of low sugar concentration (6-9 oBrix), low flow rate (0.2-0.6 ml min-1) and acidic pH (3-4). However recoveries were low at around 30% of total phenolics and selectivity was poor. It was concluded that foam fractionation represents a potential low cost technology to recover a proportion of the polyphenolic content in an enriched juice concentrate suitable for use as a functional ingredient.

Phenolic constituents of Crithmum maritimum and their radical scavenging activity

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
S Ngom ◽  
L Breant ◽  
C Antheaume ◽  
C Minker ◽  
A Leick ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Phenolic Constituents
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