scholarly journals Modeling the Dynamics of Acute Phase Protein Expression in Human Hepatoma Cells Stimulated by IL-6

Processes ◽  
2015 ◽  
Vol 3 (1) ◽  
pp. 50-70 ◽  
Author(s):  
Zhaobin Xu ◽  
Jens Karlsson ◽  
Zuyi Huang
Keyword(s):  
Protein Expression ◽  
Acute Phase ◽  
Acute Phase Protein ◽  
Hepatoma Cells ◽  
Human Hepatoma ◽  
Human Hepatoma Cells ◽  
Phase Protein

Febrile-range temperature but not heat shock augments the acute phase response to interleukin-6 in human hepatoma cells

2006 ◽  
Vol 290 (5) ◽  
pp. G903-G911 ◽  
Author(s):  
Stephen J. Wigmore ◽  
Kenneth C. H. Fearon ◽  
James A. Ross ◽  
Stephen J. McNally ◽  
William J. Welch ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Protein Synthesis ◽  
Heat Shock ◽  
Acute Phase ◽  
Acute Phase Response ◽  
Acute Phase Protein ◽  
Phase Response ◽  
Human Hepatoma ◽  
Human Hepatoma Cells ◽  
Phase Protein

The relationship between the stress protein response and the acute phase response (APPR) was studied in human hepatoma cells to investigate the hierarchy of regulation of these survival responses. Huh-7 cells were subjected to heat treatment (febrile-range temperature 40°C or heat shock 43°C) followed by recovery at 37°C in the presence or absence of IL-6 given either before or after heat treatment. The effects on total, fractional, and acute phase protein synthesis were then analyzed by metabolic labeling, ELISA, real-time PCR, Northern blot analysis, and activation of an α1-antitrypsin reporter plasmid. Cell energetics were studied under the same conditions using an index of mitochondrial activity and measurement of cellular ATP levels. Febrile-range temperature (40°C) augmented acute phase protein production when cells had been pretreated with IL-6. Pretreatment of cells with IL-6 also prevented heat shock-induced suppression of α1-antichymotrypsin (ACT) but not transferrin. mRNA expression of ACT and α1-antitrypsin reporter activation studies was consistent with transcriptional regulation of these proteins. Expression of mRNA transcripts for transferrin was increased despite protein expression being reduced by heat shock. The effects of heat shock on acute phase protein synthesis can be modified by preincubation with IL-6, whereas addition of this ligand after heat treatment has no effect on the suppressive effect of heat on the APPR. The mechanism of this action appears to be transcriptionally regulated in the case of ACT, but in the case of transferrin, it may be mediated by another process such as posttranslational modification.

scholarly journals The human hepatoma Hep3B cell line as an experimental model in the study of the long-term regulation of acute-phase proteins by cytokines

1992 ◽  
Vol 287 (1) ◽  
pp. 255-259 ◽  
Author(s):  
M Hiron ◽  
M Daveau ◽  
P Arnaud ◽  
J Bauer ◽  
J P Lebreton
Keyword(s):  
Cell Line ◽  
Acute Phase ◽  
Culture System ◽  
Acute Phase Protein ◽  
Hepatoma Cell Line ◽  
Mrna Levels ◽  
Human Hepatoma ◽  
Long Term Culture ◽  
Phase Protein

The regulation of the synthesis by the cytokines interleukin-1 (IL-1) and IL-6 of the positive acute-phase protein alpha 1-acid glycoprotein (AGP) and of the negative acute-phase protein alpha 2-HS glycoprotein (AHSG) has been studied in a long-term culture system of the human hepatoma cell line Hep3B. The culture system contained 30 nM-sodium selenite as the only supplement. This allowed maintenance of the synthesis of the proteins under study at a near steady state for over 3 months. An increase in AGP mRNA and a decrease in AHSG mRNA were observed when cells were treated for two successive 48 h-periods with monocyte-conditioned medium. A return to basal levels was obtained after cessation of the cytokine addition. Two further additions of cytokines led to alterations in mRNA levels similar to those observed following the first cytokine treatment. The amounts of AGP and AHSG secreted were altered in accordance with the mRNA modifications. These results suggest that new cytokine receptors were being constantly synthesized during cell culture. When cytokines were present in the culture medium for 10 days, maximum alterations in AGP and AHSG synthesis were obtained following 2 and 4 days of treatment respectively, but further alterations in protein levels could not be observed afterwards. Expression of IL-6 receptor mRNA was not up-regulated by cytokines, but only by 1 microM-dexamethasone. Our results show that, in this long-term culture system, cytokines induce a response in hepatoma cells similar to that observed in vivo during human inflammatory states. This model could be used to evaluate the effects of agonists or antagonists of cytokines responsible for the hepatic acute-phase protein response.

scholarly journals Gut Microbiota Is Not Involved in the Induction of Acute Phase Protein Expression Caused by Vitamin C Deficiency

2020 ◽  
Vol 66 (1) ◽  
pp. 19-23
Author(s):  
Saiko IKEDA ◽  
Saki TAKAHASHI ◽  
Norie SUZUKI ◽  
Fumiaki HANZAWA ◽  
Fumihiko HORIO ◽  
...  
Keyword(s):  
Vitamin C ◽  
Gut Microbiota ◽  
Protein Expression ◽  
Acute Phase ◽  
Acute Phase Protein ◽  
Vitamin C Deficiency ◽  
Phase Protein

Acute phase protein expression in different visceral and subcutaneous fat depots from clinically healthy dairy cows

2012 ◽  
pp. 173-176
Author(s):  
Md. Mizanur Rahman ◽  
Susanne Häussler ◽  
Manfred Mielenz ◽  
Cristina Lecchi ◽  
Fabrizio Ceciliani ◽  
...  
Keyword(s):  
Protein Expression ◽  
Dairy Cows ◽  
Acute Phase ◽  
Acute Phase Protein ◽  
Subcutaneous Fat ◽  
Fat Depots ◽  
Phase Protein

scholarly journals Cytokine and acute phase protein expression in blood samples of harbour seal pups

2008 ◽  
Vol 155 (3) ◽  
pp. 337-345 ◽  
Author(s):  
S. Fonfara ◽  
A. Kakuschke ◽  
T. Rosenberger ◽  
U. Siebert ◽  
A. Prange
Keyword(s):  
Protein Expression ◽  
Acute Phase ◽  
Acute Phase Protein ◽  
Harbour Seal ◽  
Blood Samples ◽  
Phase Protein

Proteins of rat serum IV. Time-course of acute-phase protein expression and its modulation by indomethacine

1999 ◽  
Vol 20 (4-5) ◽  
pp. 846-853 ◽  
Author(s):  
Ivano Eberini ◽  
Ingrid Miller ◽  
Valeria Zancan ◽  
Chiara Bolego ◽  
Lina Puglisi ◽  
...  
Keyword(s):  
Protein Expression ◽  
Acute Phase ◽  
Time Course ◽  
Acute Phase Protein ◽  
Rat Serum ◽  
Phase Protein
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