scholarly journals Osteogenesis of Adipose-Derived and Bone Marrow Stem Cells with Polycaprolactone/Tricalcium Phosphate and Three-Dimensional Printing Technology in a Dog Model of Maxillary Bone Defects

Polymers ◽  
2017 ◽  
Vol 9 (12) ◽  
pp. 450 ◽  
Author(s):  
Jeong Lee ◽  
Seung Chu ◽  
Hak Kim ◽  
Kang Choi ◽  
Eun Oh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Tricalcium Phosphate ◽  
Three Dimensional ◽  
Bone Defects ◽  
Bone Marrow Stem Cells ◽  
Maxillary Bone ◽  
Three Dimensional Printing ◽  
Dog Model ◽  
Dimensional Printing

Three-dimensional Printing Approaches for the Treatment of Critical-sized Bone Defects

2016 ◽  
pp. 233-278 ◽  
Author(s):  
Sara Salehi ◽  
Bilal A. Naved ◽  
Warren L. Grayson ◽  
Warren L. Grayson ◽  
Warren L. Grayson
Keyword(s):  
Three Dimensional ◽  
Bone Defects ◽  
Three Dimensional Printing ◽  
Dimensional Printing

scholarly journals Three-dimensional printing of a patient-specific engineered nasal cartilage for augmentative rhinoplasty

2019 ◽  
Vol 10 ◽  
pp. 204173141882479 ◽  
Author(s):  
Hee-Gyeong Yi ◽  
Yeong-Jin Choi ◽  
Jin Woo Jung ◽  
Jinah Jang ◽  
Tae-Ha Song ◽  
...  
Keyword(s):  
Stem Cells ◽  
Computer Aided Design ◽  
Three Dimensional ◽  
Patient Specific ◽  
Adipose Derived Stem Cells ◽  
Three Dimensional Printing ◽  
Nasal Cartilage ◽  
Computer Aided ◽  
Aided Design ◽  
Dimensional Printing

Autologous cartilages or synthetic nasal implants have been utilized in augmentative rhinoplasty to reconstruct the nasal shape for therapeutic and cosmetic purposes. Autologous cartilage is considered to be an ideal graft, but has drawbacks, such as limited cartilage source, requirements of additional surgery for obtaining autologous cartilage, and donor site morbidity. In contrast, synthetic nasal implants are abundantly available but have low biocompatibility than the autologous cartilages. Moreover, the currently used nasal cartilage grafts involve additional reshaping processes, by meticulous manual carving during surgery to fit the diverse nose shape of each patient. The final shapes of the manually tailored implants are highly dependent on the surgeons’ proficiency and often result in patient dissatisfaction and even undesired separation of the implant. This study describes a new process of rhinoplasty, which integrates three-dimensional printing and tissue engineering approaches. We established a serial procedure based on computer-aided design to generate a three-dimensional model of customized nasal implant, and the model was fabricated through three-dimensional printing. An engineered nasal cartilage implant was generated by injecting cartilage-derived hydrogel containing human adipose-derived stem cells into the implant containing the octahedral interior architecture. We observed remarkable expression levels of chondrogenic markers from the human adipose-derived stem cells grown in the engineered nasal cartilage with the cartilage-derived hydrogel. In addition, the engineered nasal cartilage, which was implanted into mouse subcutaneous region, exhibited maintenance of the exquisite shape and structure, and striking formation of the cartilaginous tissues for 12 weeks. We expect that the developed process, which combines computer-aided design, three-dimensional printing, and tissue-derived hydrogel, would be beneficial in generating implants of other types of tissue.

Microenvironment of Apatite-Fiber Scaffold Affects Cell Proliferation and Resulting Cell Differentiation

2007 ◽  
Vol 361-363 ◽  
pp. 1075-1078
Author(s):  
Michiyo Honda ◽  
Shigeki Izumi ◽  
Nobuyuki Kanzawa ◽  
Takahide Tsuchiya ◽  
Mamoru Aizawa
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Extracellular Matrix ◽  
Cell Proliferation ◽  
Cell Differentiation ◽  
Bone Marrow Cells ◽  
Three Dimensional ◽  
Bone Marrow Stem Cells ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

Appropriate culture conditions cause bone marrow stem cells to differentiate into multilineage cells such as adipocytes, chondrocytes, and osteoblasts. One key factor that regulates intercellular signaling and cell differentiation is the extracellular matrix microenvironment. The composition of the extracellular matrix influences cellular functions. In the present study, we investigated the effects of a microenvironment comprising a three-dimensional apatite-fiber scaffold (AFS) that has two kinds of pores (micro- and macro pores) on proliferation and subsequent differentiation of bone marrow stem cells. Morphologic observation revealed that osteoblastic cells in the AFS were distributed primarily in the same location on the fibrous scaffold and formed bridges within micro- and macro pores. We used molecular approaches to evaluate cell proliferation and differentiation in detail. Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that culturing bone marrow cells on AFS increases expression of osteocalcin (OC) mRNA compared with culture in a dish. Furthermore, cells cultured in AFS expressed type X collagen (Col X), which is a marker of hypertrophic cartilage. These data suggest that the three-dimensional microenvironment of AFS facilitates cell proliferation and differentiation, and promotes endochondral ossification of bone marrow cells.

Vascular differentiation of bone marrow stem cells is directed by a tunable three-dimensional matrix

2010 ◽  
Vol 6 (9) ◽  
pp. 3395-3403 ◽  
Author(s):  
Ge Zhang ◽  
Charles T. Drinnan ◽  
Laura R. Geuss ◽  
Laura J. Suggs
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Three Dimensional ◽  
Bone Marrow Stem Cells ◽  
Vascular Differentiation ◽  
Dimensional Matrix

scholarly journals Accelerating the Degradation Profile of β-Tricalcium Phosphate Bone Replacement through Three-dimensional Printing

2020 ◽  
Vol 8 (9S) ◽  
pp. 60-61
Author(s):  
Chen Shen ◽  
Maxime M. Wang ◽  
Lukasz Witek ◽  
Bruce N. Cronstein ◽  
Andrea Torroni ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Three Dimensional ◽  
Three Dimensional Printing ◽  
Bone Replacement ◽  
Dimensional Printing
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