scholarly journals Effect of Mannitol on Hyaluronic Acid Stability in Two in Vitro Models of Oxidative Stress

Polymers ◽  
2014 ◽  
Vol 6 (7) ◽  
pp. 1948-1957 ◽  
Author(s):  
Marguerite Rinaudo ◽  
Bernard Lardy ◽  
Laurent Grange ◽  
Thierry Conrozier
Keyword(s):  
Oxidative Stress ◽  
Hyaluronic Acid ◽  
In Vitro Models ◽  
Acid Stability

scholarly journals Stability of the viscoelastic properties of hyaluronic acid hydrogels with different crosslinking degrees in in vitro models of oxidative stress

2018 ◽  
Vol 26 ◽  
pp. S289-S290
Author(s):  
J.G. Prieto ◽  
B. Lázaro ◽  
A. Rodríguez ◽  
D. García ◽  
A. García ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hyaluronic Acid ◽  
Viscoelastic Properties ◽  
In Vitro Models

scholarly journals Antioxidant-Loaded Mucoadhesive Nanoparticles for Eye Drug Delivery: A New Strategy to Reduce Oxidative Stress

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 379
Author(s):  
Sandra Cordeiro ◽  
Beatriz Silva ◽  
Ana Margarida Martins ◽  
Helena Margarida Ribeiro ◽  
Lídia Gonçalves ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hyaluronic Acid ◽  
Zeta Potential ◽  
Retinal Pigment ◽  
Release Kinetic ◽  
Efficient Treatment ◽  
Non Invasive ◽  
Diffusion Cells ◽  
Franz Diffusion Cells

There are several approaches to treat ocular diseases, which can be invasive or non-invasive. Within the non-invasive, new pharmaceutical strategies based on nanotechnology and mucoadhesive polymers are emerging methodologies, which aim to reach an efficient treatment of eye diseases. The aim of this work was the development of novel chitosan/hyaluronic acid nanoparticle systems with mucoadhesive properties, intended to encapsulate antioxidant molecules (e.g., crocin) aiming to reduce eye oxidative stress and, consequently, ocular disease. An ultraviolet (UV) absorber molecule, actinoquinol, was also added to the nanoparticles, to further decrease oxidative stress. The developed nanoparticles were characterized and the results showed a mean particle size lower than 400 nm, polydispersity index of 0.220 ± 0.034, positive zeta potential, and high yield. The nanoparticles were also characterized in terms of pH, osmolality, and viscosity. Mucoadhesion studies involving the determination of zeta potential, viscosity, and tackiness, showed a strong interaction between the nanoparticles and mucin. In vitro release studies using synthetic membranes in Franz diffusion cells were conducted to unravel the drug release kinetic profile. Ex vitro studies using pig eye scleras in Franz diffusion cells were performed to evaluate the permeation of the nanoparticles. Furthermore, in vitro assays using the ARPE-19 (adult retinal pigment epithelium) cell line showed that the nanoparticles can efficiently decrease oxidative stress and showed low cytotoxicity. Thus, the developed chitosan/hyaluronic acid nanoparticles are a promising system for the delivery of antioxidants to the eye, by increasing their residence time and controlling their delivery.

Neuroprotective effects of Halimeda incrassata and Bryothamnium triquetrum aqueous extracts on in vitro models of oxidative stress

2000 ◽  
Vol 28 (5) ◽  
pp. A344-A344
Author(s):  
A. Fallarero ◽  
J. Loikkanen ◽  
O. Castaneda ◽  
A. Vidal ◽  
P. Mannisto
Keyword(s):  
Oxidative Stress ◽  
In Vitro Models ◽  
Aqueous Extracts ◽  
Neuroprotective Effects

scholarly journals Periodontitis Exacerbates Benign Prostatic Hyperplasia through Regulation of Oxidative Stress and Inflammation

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Cheng Fang ◽  
Lan Wu ◽  
Ming-Juan Zhao ◽  
Tong Deng ◽  
Jia-Min Gu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Benign Prostatic Hyperplasia ◽  
Histological Examination ◽  
Alveolar Bone ◽  
Epidemiological Studies ◽  
In Vitro Models ◽  
Prostatic Hyperplasia ◽  
Control Group ◽  
Periodontal Tissues

Epidemiological studies demonstrate that men with periodontitis are also susceptible to benign prostatic hyperplasia (BPH) and that periodontal treatment can improve the prostatic symptom. However, molecular links of this relationship are largely unknown. The goal of the current study was to elucidate the effects of experimental periodontitis on the hyperplasia of prostate and whether oxidative stress and inflammation participated in this process. For this purpose, ligature-induced periodontitis, testosterone-induced BPH, and the composite models in rats were established. Four weeks later, all the rats were sacrificed and the following items were measured: alveolar bone loss and histological examination of periodontal tissues were taken to assess the establishment of periodontitis model, prostate index and histological examination of prostate tissues were taken to test the establishment of the BPH model, inflammatory cytokines in plasma were assessed, and Bax/Bcl-2 proteins related to cell apoptosis were analyzed via western blot analysis. To further investigate whether oxidative stress participates in the aggravation of BPH, in vitro models were also conducted to measure the production of intracellular reactive oxygen species (ROS) and hydrogen peroxide (H2O2) concentration. We found that simultaneous periodontitis and BPH synergistically aggravated prostate histological changes, significantly increased Ki67 proliferation, and reduced apoptosis in rat prostate tissues. Also, our results showed that periodontal ligation induced increased Bcl-2 protein expression, whereas Bax expression was decreased in BPH rats than in normal rats. Compared with the control group, periodontitis and BPH both significantly enhanced inflammatory cytokine levels of TNF-α, IL-6, IL-1β, and CRP. Furthermore, Porphyromonas gingivalis lipopolysaccharide induced enhanced generation of intracellular expression of ROS and H2O2 in BPH-1 cells. Our experimental evidence demonstrated that periodontitis might promote BPH development through regulation of oxidative stress and inflammatory process, thus providing new strategies for prevention and treatment of BPH.

scholarly journals Modulation of Adenosine Receptors and Antioxidative Effect of Beer Extracts in in Vitro Models

Nutrients ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 1258
Author(s):  
Patricia Alonso-Andrés ◽  
Mairena Martín ◽  
José Luis Albasanz
Keyword(s):  
Oxidative Stress ◽  
Protective Effect ◽  
Adenosine Receptors ◽  
Receptor Gene ◽  
Neuroblastoma Cells ◽  
In Vitro Models ◽  
Protective Effects ◽  
Receptor Level ◽  
A1 Receptor

The fight against neurodegenerative diseases is promoting the searching of nutrients, preferably of wide consumption, with proven effects on health. Beer is widely consumed and has potential benefits on health. In this work, three different extracts from dark beer (DB), non-alcoholic beer (NAB), and lager beer (LB) were assayed at 30 min and 24 h in rat C6 glioma and human SH-SY5Y neuroblastoma cells in order to study their possible protective effects. Cell viability and adenosine A1, A2A, A2B, and A3 receptor gene expression and protein levels were measured in control cells and in cells challenged with hydrogen peroxide as an oxidant stressor. Among the three extracts analyzed, DB showed a greater protective effect against H2O2-induced oxidative stress and cell death. Moreover, a higher A1 receptor level was also induced by this extract. Interestingly, A1 receptor level was also increased by NAB and LB extracts, but to a lower extent, and the protective effect of these extracts against H2O2 was lower. This possible correlation between protection and A1 receptor level was observed at 24 h in both C6 and SH-SY5Y cells. In summary, different beer extracts modulate, to a different degree, adenosine receptors expression and protect both glioma and neuroblastoma cells from oxidative stress.

scholarly journals Characterizing the Role of Biologically Relevant Fluid Dynamics on Silver Nanoparticle Dependent Oxidative Stress in Adherent and Suspension In Vitro Models

Antioxidants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 832
Author(s):  
Katherine E. Burns ◽  
Robert F. Uhrig ◽  
Maggie E. Jewett ◽  
Madison F. Bourbon ◽  
Kristen A. Krupa
Keyword(s):  
Oxidative Stress ◽  
Fluid Dynamics ◽  
In Vitro Models ◽  
Poor Correlation ◽  
Cellular Interactions ◽  
Dynamic Flow ◽  
In Vivo Models ◽  
And Oxidative Stress

Silver nanoparticles (AgNPs) are being employed in numerous consumer goods and applications; however, they are renowned for inducing negative cellular consequences including toxicity, oxidative stress, and an inflammatory response. Nanotoxicological outcomes are dependent on numerous factors, including physicochemical, biological, and environmental influences. Currently, NP safety evaluations are carried out in both cell-based in vitro and animal in vivo models, with poor correlation between these mechanisms. These discrepancies highlight the need for enhanced exposure environments, which retain the advantages of in vitro models but incorporate critical in vivo influences, such as fluid dynamics. This study characterized the effects of dynamic flow on AgNP behavior, cellular interactions, and oxidative stress within both adherent alveolar (A549) and suspension monocyte (U937) models. This study determined that the presence of physiologically relevant flow resulted in substantial modifications to AgNP cellular interactions and subsequent oxidative stress, as assessed via reactive oxygen species (ROS), glutathione levels, p53, NFκB, and secretion of pro-inflammatory cytokines. Within the adherent model, dynamic flow reduced AgNP deposition and oxidative stress markers by roughly 20%. However, due to increased frequency of contact, the suspension U937 cells were associated with higher NP interactions and intracellular stress under fluid flow exposure conditions. For example, the increased AgNP association resulted in a 50% increase in intracellular ROS and p53 levels. This work highlights the potential of modified in vitro systems to improve analysis of AgNP dosimetry and safety evaluations, including oxidative stress assessments.

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