scholarly journals Low Molecular Weight pDMAEMA-block-pHEMA Block-Copolymers Synthesized via RAFT-Polymerization: Potential Non-Viral Gene Delivery Agents?

Polymers ◽  
2011 ◽  
Vol 3 (2) ◽  
pp. 693-718 ◽  
Author(s):  
Olga Samsonova ◽  
Christian Pfeiffer ◽  
Markus Hellmund ◽  
Olivia M. Merkel ◽  
Thomas Kissel
Keyword(s):  
Molecular Weight ◽  
Block Copolymers ◽  
Gene Delivery ◽  
Raft Polymerization ◽  
Viral Gene ◽  
Low Molecular Weight ◽  
Viral Gene Delivery

scholarly journals Amino Acid-Linked Low Molecular Weight Polyethylenimine for Improved Gene Delivery and Biocompatibility

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 975 ◽  
Author(s):  
Xiao-Ru Wu ◽  
Ji Zhang ◽  
Ju-Hui Zhang ◽  
Ya-Ping Xiao ◽  
Xi He ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Amino Acid ◽  
Gene Delivery ◽  
Transfection Efficiency ◽  
Gel Permeation Chromatography ◽  
Viral Gene ◽  
Low Molecular Weight ◽  
Ph Buffering ◽  
Gene Delivery Vectors ◽  
Viral Gene Delivery

The construction of efficient and low toxic non-viral gene delivery vectors is of great significance for gene therapy. Herein, two novel polycations were constructed via Michael addition from low molecular weight polyethylenimine (PEI) 600 Da and amino acid-containing linkages. Lysine and histidine were introduced for the purpose of improved DNA binding and pH buffering capacity, respectively. The ester bonds afforded the polymer biodegradability, which was confirmed by the gel permeation chromatography (GPC) measurement. The polymers could well condense DNA into nanoparticles and protect DNA from degradation by nuclease. Compared with PEI 25 kDa, these polymers showed higher transfection efficiency, lower toxicity, and better serum tolerance. Study of this mechanism revealed that the polyplexes enter the cells mainly through caveolae-mediated endocytosis pathway; this, together with their biodegradability, facilitates the internalization of polyplexes and the release of DNA. The results reveal that the amino acid-linked low molecular weight PEI polymers could serve as promising candidates for non-viral gene delivery.

Low molecular weight PEI-appended polyesters as non-viral gene delivery vectors

2014 ◽  
Vol 78 ◽  
pp. 118-125 ◽  
Author(s):  
Miao-Miao Xun ◽  
Yan-Hong Liu ◽  
Qian Guo ◽  
Ji Zhang ◽  
Qin-Fang Zhang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Viral Gene ◽  
Low Molecular Weight ◽  
Gene Delivery Vectors ◽  
Viral Gene Delivery

Highly Branched Poly(β-amino esters) for Non-Viral Gene Delivery: High Transfection Efficiency and Low Toxicity Achieved by Increasing Molecular Weight

2016 ◽  
Vol 17 (11) ◽  
pp. 3640-3647 ◽  
Author(s):  
Yongsheng Gao ◽  
Jian-Yuan Huang ◽  
Jonathan O’Keeffe Ahern ◽  
Lara Cutlar ◽  
Dezhong Zhou ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Transfection Efficiency ◽  
Viral Gene ◽  
Amino Esters ◽  
High Transfection Efficiency ◽  
Low Toxicity ◽  
Viral Gene Delivery
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