scholarly journals Effect of pH on the In Vitro Biocompatibility of Surfactant-Assisted Synthesis and Hydrothermal Precipitation of Rod-Shaped Nano-Hydroxyapatite

Polymers ◽  
2021 ◽  
Vol 13 (17) ◽  
pp. 2994
Author(s):  
Dan-Jae Lin ◽  
Hao-Lian Lin ◽  
Ssu-Meng Haung ◽  
Shih-Ming Liu ◽  
Wen-Cheng Chen
Keyword(s):  
Aspect Ratio ◽  
Biomedical Applications ◽  
Stable Process ◽  
In Vitro Biocompatibility ◽  
Wide Range ◽  
Alkaline Environments ◽  
Before And After ◽  
Hydrothermal Precipitation ◽  
Surfactant Assisted

Given their wide range of biomedical applications, hydroxyapatite (HA) nanoparticles are an attractive material widely used in many fields. Therefore, a simple, inexpensive, and stable process for the synthesis of HA nanoparticles is necessary to meet current needs. Herein, we studied HA synthesis assisted by four surfactants, namely cation, anion, non-ionic, and zwitterion templates, to verify the synthesis phase, aspect ratio, morphology, and biocompatibility under different environments (i.e., pH 4 and 9) before and after calcination. Results showed that before calcination, the surfactant-free groups could not produce HA but showed an abundant dicalcium phosphate anhydrous (DCPA) phase at pH 4. Except for the anionic group containing a small amount of DCPA, all surfactant-assistant groups presented single-phase HA in acidic and alkaline environments. The diameter of HA synthesized at pH 4 was significantly larger than that of HA synthesized at pH 9, and the effect of aspect ratio changes after calcination was more significant than that before calcination. The uncalcined rod-shaped HA synthesized with a non-ionic template at pH 4 demonstrated excellent cell viability, whereas anionic, cationic, and non-ionic surfactants exhibited biocompatibility only after calcination. At pH 9, non-ionic and uncalcined zwitterion-assisted rod-shaped HA showed excellent biocompatibility. In conclusion, the uncalcined HA rod-shaped nanoparticles synthesized from the non-ionic template at pH 4 and 9 and the zwitterion template at pH 9, as well as all surfactant-assisted HA after calcination, had no cytotoxicity. These tailor-made non-toxic HA types can meet the different requirements of apatite composite materials in biomedical applications.

scholarly journals Surface Properties and Biocompatibility of Anodized Titanium with a Potential Pretreatment for Biomedical Applications

Metals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1090
Author(s):  
Bai-Hung Huang ◽  
Yi-Jung Lu ◽  
Wen-Chien Lan ◽  
Muhammad Ruslin ◽  
Hung-Yang Lin ◽  
...  
Keyword(s):  
Porous Structure ◽  
Biomedical Applications ◽  
Grazing Incidence ◽  
Acid Pretreatment ◽  
Osteoblast Cells ◽  
In Vitro Biocompatibility ◽  
Porous Ti ◽  
Diphenyltetrazolium Bromide ◽  
Anodized Titanium

The effects of anodized titanium (Ti) with a potential hydrogen fluoride (HF) acid pretreatment through cathodization on the formation of nano-porous Ti dioxide (TiO2) layer were characterized using field-emission scanning electron microscopy, grazing incidence X-ray diffractometer, and contact angle goniometer. The biocompatibility was determined by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) test. Analytical results found that a well-aligned nano-porous structure was formed on the anodized Ti surface with HF pretreatment concentration above 0.5%. Microstructure of the nano-porous Ti dioxide surface generated by anodization with HF pretreatment was composed of anatase and rutile phases, while the anodized Ti sample with HF pretreatment concentration of 0.5% presented excellent hydrophilicity surface. An in-vitro biocompatibility also indicated that osteoblast cells grown on the surface of the anodized Ti sample with HF pretreatment increased with the increase of culture time. The filopodia of osteoblast cells not only adhered flat, but also tightly grabbed the nano-porous structure for promoting cell adhesion and proliferation. Therefore, the anodized Ti with HF pretreatment can form a functionalized surface with great biocompatibility for biomedical applications, particularly for dental implants.

A Review on Application of Novel Solid Nanostructures in Drug Delivery

2018 ◽  
Vol 53 ◽  
pp. 22-36 ◽  
Author(s):  
Habibollah Faraji ◽  
Reza Nedaeinia ◽  
Esmaeil Nourmohammadi ◽  
Bizan Malaekeh-Nikouei ◽  
Hamid Reza Sadeghnia ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Drug Therapy ◽  
Biomedical Applications ◽  
Imaging Biomarkers ◽  
Cellular Functions ◽  
Scientific Innovation ◽  
Wide Range ◽  
Targeted Drug

Nanotechnology as a multidisciplinary and scientific innovation plays an important role in numerous biomedical applications, such as molecular imaging, biomarkers and biosensors and also drug delivery. A wide range of studies have been conducted on using of nanoparticles for early diagnosis and targeted drug therapy of various diseases. In fact, the small size, customized surface, upgraded solubility, or multi-functionality of nanoparticles enabled them to interact with complex cellular functions in new ways which opened many doors and created new biomedical applications. These studies demonstrated that nanotechnology vehicles can formulate biological products effectively, and this nano-formulated products with a potent ability against different diseases, were represented to have better biocompatibility, bioaccessibility and efficacy, under in vitro and in vivo conditions.

scholarly journals Essential Oil Microcapsules Immobilized on Textiles and Certain Induced Effects

Materials ◽  
2019 ◽  
Vol 12 (12) ◽  
pp. 2029 ◽  
Author(s):  
Miruna S. Stan ◽  
Laura Chirila ◽  
Alina Popescu ◽  
Denisa M. Radulescu ◽  
Diana E. Radulescu ◽  
...  
Keyword(s):  
Membrane Integrity ◽  
Dermal Fibroblasts ◽  
Cell Membrane Integrity ◽  
Textile Materials ◽  
Skin Cells ◽  
In Vitro Biocompatibility ◽  
Short Term Exposure ◽  
Before And After

In order to obtain textile materials with potential utility in the development of cosmetic textiles, this study examined the deposition by padding of rose and sage microcapsules on woven textile structures, with different fiber compositions (100% cotton and 50% cotton/50% polyester). Cationization of the textile materials was performed to enhance the degree of uptake the pf the microcapsules on the fabrics’ surface. A commercially acrylate-based binder was used to fix the microcapsules to the textile substrate and to improve the durability against external factors. The finished textile materials were characterized in terms of their physical-mechanical characteristics. The distribution of microcapsules on the fabrics surface before and after five washing cycles and 1000 abrasion cycles was investigated by scanning electron microscopy. The biocompatibility in terms of cell viability, cell membrane integrity and inflammation status of the functionalized fabrics was evaluated on CCD-1070Sk normal human dermal fibroblasts. The cell morphology was evaluated by F-actin staining using fluorescence microscopy and no significant changes were noticed after the incubation in the presence of fabrics compared with control. The in vitro biocompatibility evaluation on human skin cells confirmed the absence of cytotoxicity after the short-term exposure, supporting further in vivo use of these innovative textiles with improved properties.

Design Considerations and Assays for Hemocompatibility of FDA-Approved Nanoparticles

2019 ◽  
Vol 46 (05) ◽  
pp. 637-652
Author(s):  
Amit K. Saha ◽  
Min-Yi S. Zhen ◽  
Folarin Erogbogbo ◽  
Anand K. Ramasubramanian
Keyword(s):  
Biomedical Applications ◽  
Inflammatory Responses ◽  
Mode Of Delivery ◽  
Systemic Circulation ◽  
In Vitro Assays ◽  
Cardiovascular Tissues ◽  
Wide Range ◽  
The Us ◽  
Imaging Sensors

AbstractNanoparticles have numerous biomedical applications including, but not limited to, targeted drug delivery, diagnostic imaging, sensors, and implants for a wide range of diseases including cancer, diabetes, heart disease, and tuberculosis. Although the mode of delivery of the nanoparticles depends on the application and the disease, the nanoparticles are often in immediate contact with the systemic circulation either because of intravenous administration or their ability to enter the bloodstream with relative ease or their longer survival time in circulation. Once in circulation, the nanoparticles may elicit unintended hemostatic and inflammatory responses, and hence the design of nanoparticles for therapeutic applications should take broad hemocompatibility concerns into consideration. In this review, we present the principles underlying the structural and functional design of various classes of nanoparticles that are currently approved by the US Food and Drug Administration, categorize these particles based on their interactions with cardiovascular tissues and ensuing adverse events, and also describe various in vitro assays that may be used evaluate their hemocompatibility.

3D Printed PLA/PCL/TiO2 Composite for Bone Replacement and Grafting

MRS Advances ◽  
2018 ◽  
Vol 3 (40) ◽  
pp. 2373-2378 ◽  
Author(s):  
Sandra E. Nájera ◽  
Monica Michel ◽  
Nam-Soo Kim
Keyword(s):  
Biomedical Applications ◽  
Fused Deposition Modeling ◽  
Fracture Strain ◽  
Fused Deposition ◽  
In Vitro Biocompatibility ◽  
Bone Replacement ◽  
Deposition Modeling ◽  
3D Printed ◽  
The Stability

ABSTRACTPolymer composites of Polylactic acid (PLA) and poly-ε-caprolactone (PCL), containing small amounts of titanium oxide (TiO2) were developed for biomedical applications. These composite materials were prepared, and then printed using Fused Deposition Modeling (FDM). 3D printed structures were characterized to determine their mechanical properties and biocompatibility. DSC analysis yielded useful information regarding the immiscibility of the different polymers, and it was observed that the particles of TiO2 improved the stability of the polymers. The ultimate tensile strength and the fracture strain increased by adding TiO2 as a filler, resulting in values of approximately 45 MPa and 5.5 % elongation. The printed composites show excellent in vitro biocompatibility including cell proliferation and adhesion, and are therefore promising candidates to be used in the biomedical field for bone replacement procedures, due to their properties similar to those of cancellous bone.

Enhanced Biocompatibility of GPC by Ion Implantation and Deposition

2005 ◽  
Vol 908 ◽  
Author(s):  
Robert Lee Zimmerman ◽  
Ismet Gürhan ◽  
Claudiu I. Muntele ◽  
Daryush Ila ◽  
Feyzan Özdal-Kurt ◽  
...  
Keyword(s):  
Ion Implantation ◽  
Biomedical Applications ◽  
Heart Valves ◽  
Cell Attachment ◽  
Blood Stream ◽  
Silver Deposition ◽  
In Vitro Biocompatibility ◽  
Artificial Heart Valves ◽  
Model Cell

AbstractBiocompatible Glassy Polymeric Carbon (GPC) is used for artificial heart valves and in other biomedical applications. Although it is ideally suited for implants in the blood stream, tissue that normally forms around the moving parts of a GPC heart valve sometimes loses adhesion and creates embolisms downstream. Here we compare silver ion implantation and silver deposition, each of which strongly inhibits cell attachment on GPC. Inhibition of cell adhesion is a desirable improvement to current GPC cardiac implants. In vitro biocompatibility tests have been carried out with model cell lines to demonstrate that traces of silver can favorably influence the surface of GPC for biomedical applications.

In Vitro Examination of Poly(glycerol sebacate) Degradation Kinetics: Effects of Porosity and Cure Temperature

2014 ◽  
Vol 1621 ◽  
pp. 87-92 ◽  
Author(s):  
Nadia M. Krook ◽  
Courtney LeBlon ◽  
Sabrina S. Jedlicka
Keyword(s):  
Biomedical Applications ◽  
Degradation Kinetics ◽  
Morphological Changes ◽  
Scanning Calorimetry ◽  
Tissue Engineering Scaffolds ◽  
Degradation Study ◽  
Wide Range ◽  
Thermal Changes ◽  
Cure Temperature

ABSTRACTPoly(glycerol sebacate) (PGS) is a biodegradable and biocompatible elastomer that has been used in a wide range of biomedical applications. While a porous format is common for tissue engineering scaffolds, to allow cell ingrowth, PGS degradation has been primarily studied in a nonporous format. The purpose of this research was to investigate the degradation of porous PGS at three frequently used cure temperatures: 120°C, 140°C, and 165°C. The thermal, chemical, mechanical, and morphological changes were examined using thermogravimetric analysis, differential scanning calorimetry, Fourier transform infrared spectroscopy, compression testing, and scanning electron microscopy. Over the course of the 16-week degradation study, the samples’ pores collapsed. The specimens cured at 120°C demonstrated the most degradation and became gel-like after 16 weeks. Thermal changes were most evident in the 120°C and 140°C cure PGS specimens, as shifts in the melting and recrystallization temperatures occurred. Porous samples cured at all three temperatures displayed a decrease in compressive modulus after 16 weeks. This in vitro study helped to elucidate the effects of porosity and cure temperature on the biodegradation of PGS and will be valuable for the design of future PGS scaffolds.

scholarly journals Thymodepressin—Unforeseen Immunosuppressor

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6550
Author(s):  
Vladislav I. Deigin ◽  
Julia E. Vinogradova ◽  
Dmitry L Vinogradov ◽  
Marina S. Krasilshchikova ◽  
Vadim T. Ivanov
Keyword(s):  
Experimental Evidence ◽  
Synthetic Peptide ◽  
Biomedical Applications ◽  
Biological Properties ◽  
Peptide Drug ◽  
Wide Range ◽  
History Of ◽  
Available Information

The paper summarizes the available information concerning the biological properties and biomedical applications of Thymodepressin. This synthetic peptide drug displays pronounced immunoinhibitory activity across a wide range of conditions in vitro and in vivo. The history of its unforeseen discovery is briefly reviewed, and the current as well as potential expansion areas of medicinal practice are outlined. Additional experimental evidence is obtained, demonstrating several potential advantages of Thymodepressin over another actively used immunosuppressor drug, cyclosporin A.

scholarly journals Oxytetracycline versus Doxycycline Collagen Sponges Designed as Potential Carrier Supports in Biomedical Applications

Pharmaceutics ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 363 ◽  
Author(s):  
Tihan ◽  
Rău ◽  
Zgârian ◽  
Ungureanu ◽  
Barbaresso ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Structural Integrity ◽  
Release Kinetics ◽  
Collagen Sponge ◽  
Application Site ◽  
Cross Linking ◽  
Drug Kinetic ◽  
In Vitro Biocompatibility ◽  
Kinetic Release

Many research studies are directed toward developing safe and efficient collagen-based biomaterials as carriers for drug delivery systems. This article presents a comparative study of the properties of new collagen sponges prepared and characterized by different methods intended for biomedical applications. The structural integrity is one of the main properties for a biomaterial in order for it to be easily removed from the treated area. Thus, the effect of combining a natural polymer such as collagen with an antimicrobial drug such as oxytetracycline or doxycycline and glutaraldehyde as the chemical cross-linking agent influences the cross-linking degree of the material, which is in direct relation to its resistance to collagenase digestion, the drug kinetic release profile, and in vitro biocompatibility. The enzymatic degradation results identified oxytetracycline as the best inhibitor of collagenase when the collagen sponge was cross-linked with 0.5% glutaraldehyde. The drug release kinetics revealed an extended release of the antibiotic for oxytetracycline-loaded collagen sponges compared with doxycycline-loaded collagen sponges. Considering the behavior of differently prepared sponges, the collagen sponge with oxytetracycline and 0.5% glutaraldehyde could represent a viable polymeric support for the prevention/treatment of infections at the application site, favoring tissue regeneration.

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