scholarly journals Succinylation of Polyallylamine: Influence on Biological Efficacy and the Formation of Electrospun Fibers

Polymers ◽  
2021 ◽  
Vol 13 (17) ◽  
pp. 2840
Author(s):  
Lucija Jurko ◽  
Matej Bračič ◽  
Silvo Hribernik ◽  
Damjan Makuc ◽  
Janez Plavec ◽  
...  
Keyword(s):  
Wound Dressing ◽  
Inhibitory Concentration ◽  
Water Soluble ◽  
Primary Amines ◽  
Fibroblast Cells ◽  
Amino Acid Residues ◽  
Ph Values ◽  
Absorbance Spectroscopy ◽  
Amine Groups ◽  
Dressing Materials

Succinylation of proteins is a commonly encountered reaction in biology and introduces negatively charged carboxylates on previously basic primary amine groups of amino acid residues. In analogy, this work investigates the succinylation of primary amines of the synthetic polyelectrolyte polyallylamine (PAA). It investigates the influence of the degree of succinylation on the cytotoxicity and antibacterial activity of the resulting polymers. Succinylation was performed in water with varying amounts of succinic anhydride and at different pH values. The PAA derivatives were analyzed in detail with respect to molecular structure using nuclear magnetic resonance and infrared absorbance spectroscopy. Polyelectrolyte and potentiometric charge titrations were used to elucidate charge ratios between primary amines and carboxylates in the polymers. The obtained materials were then evaluated with respect to their minimum inhibitory concentration against Staphylococcus aureus and Pseudomonas aeruginosa. The biocompatibility was assessed using mouse L929 fibroblasts. The degree of succinylation decreased cytotoxicity but more significantly reduced antibacterial efficacy, demonstrating the sensitivity of the fibroblast cells against this type of ampholytic polyelectrolytes. The obtained polymers were finally electrospun into microfiber webs in combination with neutral water-soluble polyvinyl alcohol. The resulting non-woven could have the potential to be used as wound dressing materials or coatings.

Development for Wound Dressing Based on Blended Chitosan and Gelatin Hydrogels

2018 ◽  
Vol 765 ◽  
pp. 119-123 ◽  
Author(s):  
I Hao Chen ◽  
Ching Ming Chien ◽  
Chun Ting Wang ◽  
Chih Ling Huang ◽  
Chih Kuang Wang ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Wound Dressing ◽  
Body Fluid ◽  
Raw Materials ◽  
Fibroblast Cells ◽  
Cell Toxicity ◽  
Thermogravimetric Analyzer ◽  
Negative Effect ◽  
Dressing Materials ◽  
Degree Of Degradation

Chitosan and gelatin are potential wound dressing materials. However, chitosan takes too much time to degrade and gelatin degrades too fast. As a result, chitosan and gelatin are blended to adjust their degradation rate. The degree of degradation test by means of weight loss and thermogravimetric analyzer after being incubated with lysozyme, which is a common enzyme in body fluid. Optical microscopy was used to observe the materials morphology after immersed in water. Finally, fibroblast cells were cultured with various material extracts to examine cell adhesion In Vitro. Cell adhesion tests showed there are no negative effect on cells. Therefore, there is no cell toxicity of chitosan on cells. Chitosan and gelatin can be promising wound dressing raw materials in future.

Investigation of Dialdehyde Corn Starch as Crosslinking Agent in Collagen-Based Wound Dressing Materials

2016 ◽  
Author(s):  
Cigdem Kilicarislan Ozkan ◽  
Hasan Ozgunay ◽  
Stefania Marin ◽  
Madalina Georgiana Albu Kaya
Keyword(s):  
Wound Dressing ◽  
Corn Starch ◽  
Crosslinking Agent ◽  
Dressing Materials ◽  
Wound Dressing Materials

scholarly journals Breathable, stretchable and adhesive nanofibrous hydrogels as wound dressing materials

2021 ◽  
Author(s):  
Xueting Liu ◽  
Yumin Liu ◽  
Jingtao Du ◽  
Xiaoran Li ◽  
Jianyong Yu ◽  
...  
Keyword(s):  
Wound Dressing ◽  
Dressing Materials ◽  
Wound Dressing Materials

Antifouling Antioxidant Zwitterionic Dextran Hydrogels as Wound Dressing Materials with Excellent Healing Activities

2021 ◽  
Vol 13 (6) ◽  
pp. 7060-7069
Author(s):  
Xia Qiu ◽  
Jiamin Zhang ◽  
Lilong Cao ◽  
Qin Jiao ◽  
Junhao Zhou ◽  
...  
Keyword(s):  
Wound Dressing ◽  
Dressing Materials ◽  
Wound Dressing Materials

Injectable keratin hydrogels as hemostatic and wound dressing materials

2021 ◽  
Author(s):  
Ao Tang ◽  
Ying Li ◽  
Yiqian Yao ◽  
Xuexia Yang ◽  
Zhangjun Cao ◽  
...  
Keyword(s):  
Wound Dressing ◽  
Biomedical Applications ◽  
Injectable Hydrogels ◽  
Good Biocompatibility ◽  
Dressing Materials ◽  
Administration Procedure ◽  
Wound Dressing Materials

Injectable hydrogels hold promise in biomedical applications due to their noninvasive administration procedure, and capacity enabling the filling of irregularly shaped defects. Protein-based hydrogels provide features including good biocompatibility and...

scholarly journals Correction: On-demand removable hydrogels based on photolabile cross-linkings as wound dressing materials

2021 ◽  
Vol 9 (10) ◽  
pp. 2548-2548
Author(s):  
Haiyang Wu ◽  
Zezhao Qin ◽  
Xiaofeng Yu ◽  
Jinge Li ◽  
Hongying Lv ◽  
...  
Keyword(s):  
Wound Dressing ◽  
On Demand ◽  
Dressing Materials ◽  
Wound Dressing Materials

Correction for ‘On-demand removable hydrogels based on photolabile cross-linkings as wound dressing materials’ by Haiyang Wu et al., J. Mater. Chem. B, 2019, 7, 5669–5676, DOI: 10.1039/C9TB01544B.

Biosynthesis of Carboxymethylated Bacterial Cellulose Composite for Wound Dressing

2011 ◽  
Vol 685 ◽  
pp. 322-326 ◽  
Author(s):  
Jun Wei Yu ◽  
Xiao Li Liu ◽  
Chang Sheng Liu ◽  
Dong Ping Sun
Keyword(s):  
Bacterial Cellulose ◽  
Wound Dressing ◽  
Culture Medium ◽  
Acetobacter Xylinum ◽  
Water Holding Capacity ◽  
Water Soluble ◽  
Transmission Rates ◽  
Measurement Results ◽  
Water Holding ◽  
Cellulose Composite

A novel bacterial cellulose (BC) composite (carboxymethylated-bacterial cellulose, CM-BC) was synthesized by Acetobacter xylinum by adding water-soluble carboxymethylated cellulose (CMC) in the culture medium. FTIR results showed that CM-BC is obtained by the incorporation of CMC in the network of BC. Water-holding capacity and water vapor transmission rates (WVTR) of CM-BC and BC are determined. The WVTR of CM-BC is comparable to that of BC, but the water-holding capacity of CM-BC is improved compared with BC. Tensile strengths measurement results showed that the fracture stress of CM-BC is higher than that of BC, indicating that CM-BC have more potential wound dressing applications than BC.

Nanofiber Wound Dressing Materials—A Comparative Study of Wound Healing on a Porcine Model

2021 ◽  
Author(s):  
Katerina Menclová ◽  
Petr Svoboda ◽  
Jan Hadač ◽  
Štefan Juhás ◽  
Jana Juhásová ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Blood Count ◽  
Serum Amyloid A ◽  
Porcine Model ◽  
Serum Amyloid ◽  
Wound Dressings ◽  
Amyloid A ◽  
Inflammatory Phase ◽  
Dressing Materials

ABSTRACT Background Nanofiber wound dressings remain the domain of in vitro studies. The purpose of our study was to verify the benefits of chitosan (CTS) and polylactide (PLA)-based nanofiber wound dressings on a porcine model of a naturally contaminated standardized wound and compare them with the conventional dressings, i.e., gauze and Inadine. Material and Methods The study group included 32 pigs randomized into four homogeneous groups according to the wound dressing type. Standardized wounds were created on their backs, and wound dressings were regularly changed. We evaluated difficulty of handling individual dressing materials and macroscopic appearance of the wounds. Wound swabs were taken for bacteriological examination. Blood samples were obtained to determine blood count values and serum levels of acute phase proteins (serum amyloid A, C-reactive protein, and haptoglobin). The crucial point of the study was histological analysis. Microscopic evaluation was focused on the defect depth and tissue reactions, including formation of the fibrin exudate with neutrophil granulocytes, the layer of granulation and cellular connective tissue, and the reepithelialization. Statistical analysis was performed by using SPSS software. The analysis was based on the Kruskal–Wallis H test and Mann–Whitney U test followed by Bonferroni correction. Significance was set at P < .05. Results Macroscopic examination did not show any difference in wound healing among the groups. However, evaluation of histological findings demonstrated that PLA-based nanofiber dressing accelerated the proliferative (P = .025) and reepithelialization (P < .001) healing phases, while chitosan-based nanofiber dressing potentiated and accelerated the inflammatory phase (P = .006). No statistically significant changes were observed in the blood count or acute inflammatory phase proteins during the trial. Different dynamics were noted in serum amyloid A values in the group treated with PLA-based nanofiber dressing (P = .006). Conclusion Based on the microscopic examination, we have documented a positive effect of nanofiber wound dressings on acceleration of individual phases of the healing process. Nanofiber wound dressings have a potential to become in future part of the common wound care practice.

A STUDY OF A WATER-SOLUBLE COMPLEX OF PRODIGIOSIN PRODUCED BY A STRAIN OF SERRATIA MARCESCENS

1962 ◽  
Vol 40 (1) ◽  
pp. 1019-1024 ◽  
Author(s):  
Seiichi Yoshida
Keyword(s):  
Molecular Weight ◽  
Light Scattering ◽  
Serratia Marcescens ◽  
Average Molecular Weight ◽  
Electrophoretic Analysis ◽  
Water Soluble ◽  
Soluble Complex ◽  
Ph Values ◽  
Scattering Measurements ◽  
Water Soluble Complex

A water-soluble pigment excreted from Serratia marcescens has been purified by precipitation with ammonium sulphate, dialysis, and ultracentrifugation at different pH values. The purified pigment showed a single band in the ultracentrifuge and by electrophoretic analysis at several pH values. An average molecular weight of 5 × 106 was calculated from light-scattering measurements. This pigment is composed of carbohydrate and protein combined with prodigiosin, and several properties of the complex are described.

scholarly journals A handy skin wound dressing prepared by alginate and cationic nanofibrillated cellulose derived from solid residues of herbs

BioResources ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. 5926-5946
Author(s):  
Ting Jiang ◽  
Xiaoyan Feng ◽  
Rui Xu ◽  
Sheng Dong ◽  
Meiyan Wu ◽  
...  
Keyword(s):  
Wound Dressing ◽  
Cost Effective ◽  
Nanofibrillated Cellulose ◽  
Antibacterial Activities ◽  
Skin Wound ◽  
Natural Medicine ◽  
Nanofibrous Structure ◽  
New Strategy ◽  
Physical Interactions ◽  
Dressing Materials

Large amounts of solid residues are generated after extraction of active ingredients from herbs for the production of natural medicine, but the residues have not been well utilized. In this work, cationic nanofibrillated cellulose (CCNF) was prepared from the solid residues of Astragali Radix by etherification and homogenization. The CCNF was mixed with sodium alginate (SA) to create a hydrogel dressing by physical interactions between CCNF and SA without any addition of cross-linker. The CCNF-SA dressing exhibited moderate viscosity, good moisture-maintaining property, great antibacterial activities, good cytocompatibility, and clear acceleration of wound healing on rats. Furthermore, this CCNF-SA dressing with nanofibrous structure had moderate air permeability. Therefore, the CCNF-SA hydrogel could be used potentially as a skin wound dressing. Development of cost-effective and bioactive wound dressing materials is of crucial importance to reduce the burden on patients and healthcare systems. Also, this work provides a new strategy for valorization of the solid residues of herbs.

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