scholarly journals Evaluation of Physical, Mechanical and Antibacterial Properties of Pinto Bean Starch-Polyvinyl Alcohol Biodegradable Films Reinforced with Cinnamon Essential Oil

Polymers ◽  
2021 ◽  
Vol 13 (16) ◽  
pp. 2778
Author(s):  
Ali Khazaei ◽  
Leila Nateghi ◽  
Nazanin Zand ◽  
Abdulrasoul Oromiehie ◽  
Farhad Garavand
Keyword(s):  
Essential Oil ◽  
Polyvinyl Alcohol ◽  
Antibacterial Properties ◽  
Scanning Electron Micrographs ◽  
X Ray Diffraction ◽  
Gram Positive Bacteria ◽  
Control Film ◽  
Pinto Bean ◽  
Cinnamon Essential Oil ◽  
Xrd Patterns

In the present study, various blended films from polyvinyl alcohol (PVA) and pinto bean starch (PBS) were prepared and the selected film was used to fabricate an antimicrobial packaging film. Different essential oils (EOs) were also exposed to minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests to find the most efficient EO against a range of microorganisms. From the primary studies, the PVA:PBS (80:20) and cinnamon essential oil (CEO) were chosen. Afterward, the blend composite film reinforced by 1, 2, and 3% CEO and several, physical, mechanical, structural, and antimicrobial attributes were scrutinized. The results showed a significant modification of the barrier and mechanical properties of the selected blended films as a result of CEO addition. Scanning electron micrographs confirmed the incorporation and distribution of CEO within the film matrix. The X-ray diffraction (XRD) patterns and Fourier transform infrared (FTIR) spectra indicated the interaction of CEO and the PVA-PBS composite. The antibacterial of the tested bacteria showed a significant increase by increasing the CEO concentration within the control film. CEO-loaded films were more effective in controlling Gram-positive bacteria compared to Gram-negative bacteria. It can be concluded that PVA-PBS-CEO films are promising candidates to produce biodegradable functional films for food and biomedical applications.

scholarly journals Edible Films from Carrageenan/Orange Essential Oil/Trehalose—Structure, Optical Properties, and Antimicrobial Activity

Polymers ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 332
Author(s):  
Jancikova Simona ◽  
Dordevic Dani ◽  
Sedlacek Petr ◽  
Nejezchlebova Marcela ◽  
Treml Jakub ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Antibacterial Properties ◽  
Edible Films ◽  
Attenuated Total Reflectance ◽  
Practical Application ◽  
Film Properties ◽  
Gram Positive Bacteria ◽  
Edible Packaging ◽  
Orange Essential Oil

The research aim was to use orange essential oil and trehalose in a carrageenan matrix to form edible packaging. The edible packaging experimentally produced by casting from an aqueous solution were evaluated by the following analysis: UV-Vis spectrum, transparency value, transmittance, attenuated total reflectance Fourier-Transform spectroscopy (FTIR), scanning electron microscopy (SEM) and antimicrobial activity. The obtained results showed that the combination of orange essential oil with trehalose decreases the transmittance value in the UV and Vis regions (up to 0.14% ± 0.02% at 356 nm), meaning that produced films can act as a UV protector. Most produced films in the research were resistant to Gram-positive bacteria (Staphylococcus aureus subsp. aureus), though most films did not show antibacterial properties against Gram-negative bacteria and yeasts. FTIR and SEM confirmed that both the amount of carrageenan used and the combination with orange essential oil influenced the compatibility of trehalose with the film matrix. The research showed how different combinations of trehalose, orange essential oils and carrageenan can affect edible film properties. These changes represent important information for further research and the possible practical application of these edible matrices.

scholarly journals Antibacterial and antibiofilm activities of cinnamon essential oil nanoemulsion against multi-species oral biofilms

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yeo-Jin Jeong ◽  
Hee-Eun Kim ◽  
Su-Jin Han ◽  
Jun-Seon Choi
Keyword(s):  
Essential Oil ◽  
Repeated Measures ◽  
Antibacterial Properties ◽  
Three Solutions ◽  
Bacterial Colony ◽  
Biofilm Model ◽  
Oral Biofilms ◽  
Cinnamon Essential Oil ◽  
Cocamidopropyl Betaine ◽  
Bovine Enamel

AbstractCinnamon essential oil (CEO) has antibacterial properties, but its ability to suppress the formation of multi-species oral biofilms has not been fully elucidated. This study evaluated the antibacterial and antibiofilm activities of cinnamon essential oil nanoemulsion (CEON) against oral biofilms formed using a microcosm biofilm model. The biofilms were formed on bovine enamel specimens over a 7-day period, during which all specimens were treated with one of three solutions: 5% CEON (n = 35), 0.5% cocamidopropyl betaine (n = 35), or 0.12% chlorhexidine gluconate (CHX; n = 35). Antibacterial and antibiofilm activities were determined by the red/green ratios (R/G values) of 7-day-old mature biofilms photographed with quantitative light-induced fluorescence-digital, the number of aciduric bacterial colony-forming units (CFUs) within each biofilm, and the absorbance of bacterial suspensions. One-way and repeated-measures analysis of variance were performed to compare differences among the three solutions. R/G values were lowest in the 0.12% CHX group, but not significantly differ from the 5% CEON group. The number of CFUs and absorbance were lowest in the 5% CEON group. This study showed that nanoemulsified CEO inhibited the maturation of multi-species oral biofilms and the growth of oral microorganisms in biofilms, including aciduric bacteria that cause dental caries.

scholarly journals The Effects of Copper Addition on The Structure and Antibacterial Properties of Biomedical Glasses

2020 ◽  
Author(s):  
Leyla Mojtabavi ◽  
Amir Razavi
Keyword(s):  
Antibacterial Properties ◽  
Inhibition Zone ◽  
X Ray Diffraction ◽  
Network Modifier ◽  
X Ray ◽  
E Coli ◽  
Copper Addition ◽  
Xrd Patterns ◽  
Copper Incorporation ◽  
Glass Compositions

AbstractIn this work, we studied the effects of copper incorporation in the composition of bioactive glass. Three different glass compositions were synthesized with 0, 3, and 6 mol% of copper addition. X-Ray Diffraction (XRD) patterns confirmed that an amorphous microstructure was obtained for all three glass compositions. Results from Differential Thermal Analysis (DTA) showed that the copper addition in the glass lowers the glass transition temperature, from 646°C to 590°C when added at 6 mol%. X-ray Photoelectron (XPS) survey and high-resolution scans were performed to study the structural effects of copper addition in the glass. Results indicated that the incorporation of copper changes the ratio of bridging to non-birding oxygens in the structure. Glasses were further analyzed for their structure with Nuclear Magnetic Resonance (NMR) spectroscopy, which indicated that copper acts as a network modifier in the glass composition and copper-containing glasses show a less connected microstructure. Antibacterial efficacy of the glasses was analyzed against E. coli and S. epidermis. Copper-containing glasses showed a significantly higher inhibition zone compared to control glass. The glass with 6 mol% copper, exhibited inhibition zones of 9 and 16mm against E. coli and S. epidermis bacteria, respectively.

scholarly journals Process Optimization of Microwave Assisted Simultaneous Distillation and Extraction from Siam cardamom using Response Surface Methodology

Processes ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 449
Author(s):  
Panawan Suttiarporn ◽  
Nalin Wongkattiya ◽  
Kittisak Buaban ◽  
Pisit Poolprasert ◽  
Keerati Tanruean
Keyword(s):  
Response Surface Methodology ◽  
Essential Oil ◽  
Response Surface ◽  
Leaf Sheath ◽  
Scanning Electron Micrographs ◽  
Effective Technique ◽  
Box Behnken Design ◽  
Quadratic Equations ◽  
Gram Positive Bacteria ◽  
Microwave Assisted

The main goal of the research was to optimize microwave-assisted simultaneous distillation and extraction (MA-SDE) using response surface methodology (RSM), based on Box–Behnken design (BBD). A process was designed to extract the essential oil from the leaf sheath of Siam cardamom. The experimental data were fitted to quadratic equations, and the experiment conditions for optimal extraction of 1,8-cineole were extraction time 87.68 min, material-to-water ratio 1:13.18 g/mL and microwave power 217.77 W. Under such conditions, the content of 1,8-cineole was 157.23 ± 4.23 µg/g, which matched with the predicted value. GC–MS results indicated the presence of predominant oxygenated monoterpenes including 1,8-cineole (20.63%), iso-carveol (14.30%), cis-p-mentha-1(7),8-dien-2-ol (12.27%) and trans-p-2,8-menthadien-1-ol (9.66%), and oxygenated contents were slightly higher in the MA-SDE and extraction compared to usual SDE. In addition, the essential oil extracted by MA-SDE exhibited strong antibacterial effects against the tested Gram-positive bacteria. Scanning electron micrographs provided more evidence of destruction of the leaf sheath treated by MA-SDE. Conclusively, microwave-assisted simultaneous distillation and solvent extraction appear to be an effective technique for the separation of essential oils enriched 1,8-cineole from Siam cardamom leaf sheath in a shorter time.

scholarly journals Chemical Indices and Antibacterial Properties of Some Essential Oils of Apiaceae and Lauraceae Spices in Southwest of Algeria

2019 ◽  
Author(s):  
Dalila Razni ◽  
Linda Rouisset ◽  
Elhassan Benyagoub
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Enterococcus Faecalis ◽  
Antibacterial Properties ◽  
Independent Effect ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Cinnamon Essential Oil ◽  
The One

This study is a part of the valorization of extract from three most commonly used Algerian spices, namely; caraway and cumin seeds and cinnamon bark. On the one hand, it aims at characterizing the chemical indices of extracted essential oils and evaluating the antibacterial activity of each essential oil by titration and disc diffusion method respectively. On the other hand, it attempts at evaluating the combined action of essential oils against four reference pathogenic bacterial strains, namely Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Enterococcus faecalis by well and Chabbert-type diffusion method. The essential oils obtained by the hydrodistillation method have a relatively average extraction about 1.43, 2.3 and 2.5%, respectively for caraway, cumin, and cinnamon. The acid index indicates the behavior and amount of free acids present in the essential oil, in which the acid and saponification indices of cinnamon essential oil indicate a value of 4.48 and 168.56 respectively. It can also inform us about the susceptibility of the oil to undergo alterations. The antibacterial activity results showed that cinnamon essential oil (EO) proved to be the most active against the tested bacterial strains; caraway EO was active against Enterococcus faecalis, and the antibacterial action of cumin EO was the lowest. However, the association of the extracted essential oils has a higher synergistic effect than the independent effect of each essential oil, in which the MIC value found was estimated at 10 to 20 (V/V), 40 to 50 (V/V) and 50 to 70 (V/V) respectively for cinnamon, cumin and caraway. The obtained results show that the response to the antibacterial activity varies according to the plant species used and the extract tested alone or in combination.

scholarly journals Alginate Biocomposite Films Incorporated with Cinnamon Essential Oil Nanoemulsions: Physical, Mechanical, and Antibacterial Properties

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Kporwodu Frank ◽  
Coralia V. Garcia ◽  
Gye Hwa Shin ◽  
Jun Tae Kim
Keyword(s):  
Tensile Strength ◽  
Essential Oil ◽  
Antibacterial Properties ◽  
Tween 80 ◽  
Probe Type ◽  
Elongation At Break ◽  
Biocomposite Films ◽  
Inhibition Zones ◽  
Cinnamon Essential Oil ◽  
Fresh Foods

Alginate-based antibacterial biocomposite films were prepared by incorporating with cinnamon essential oil nanoemulsions (CEO-NE). CEO-NE was first prepared by mixing and homogenizing the oil phase and aqueous phase containing polysorbate 80 (Tween 80), using a probe-type ultrasonication equipment. The biocomposite films were then prepared by incorporating the CEO-NE to an aqueous solution of alginate and glycerol, homogenizing, casting, and drying. The mean droplet size, zeta potential, and polydispersity index of the CEO-NE were 92.2 nm, −15.58 mV, and 0.25, respectively. Young’s modulus of the CEO-NE/alginate biocomposite films was significantly increased with an increasing concentration of CEO-NE, while their elongation at break was significantly decreased. However, the tensile strength of the CEO-NE/alginate biocomposite films was not significantly changed. The maximum tensile strength and elongation at break of the biocomposite films were 15.63 MPa and 23.67%, respectively, corresponding to the biocomposite films containing 20% CEO-NE. The biocomposite films containing 20% CEO-NE also showed strong antibacterial effects against Salmonella typhimurium, Bacillus cereus, Escherichia coli, and Staphylococcus aureus, achieving inhibition zones from 29.7 to 53.0 mm. These results show the potential of the CEO-NE/alginate biocomposite films as antibacterial packaging for extending the shelf life of fresh foods.

Antibacterial Effects of Oxide Compound: The Case Study of Agricultural Waste Material

2014 ◽  
Vol 979 ◽  
pp. 327-330
Author(s):  
W. Siriprom ◽  
K. Teanchai
Keyword(s):  
Agricultural Waste ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Waste Material ◽  
X Ray Diffraction ◽  
Oxide Compound ◽  
X Ray ◽  
Diffusion Technique ◽  
Structure Morphology ◽  
Xrd Patterns

The aim of this work investigated the antibacterial properties of oxide compound from agricultural waste material. The oxide compounds were prepared by annealed the agricultural waste material upon consequence of temperature. After that, the crystalline structure, morphology, chemical composition and estimation of oxide compound were conducted by X-Ray Diffraction (XRD), Energy Dispersive X-Ray Fluorescence (EDXRF) and Fourier Transform Infrared Spectroscopy (FTIR). It was observed that the XRD patterns demonstrated the lime phase and cristobalite phase after heat treatment. Another that, the result from EDXRF and FTIR was in good agreement with the result of XRD. For anti-bacterial efficacy were utilized the lime phase and cristobalite phase for testing antibacterial activities which the agar diffusion technique. It was found that the inhibition zones were clearly visible for both agents, found to exhibit antibacterial action against Escherichia coli.

scholarly journals Phytochemical characterization, and antioxidant and antimicrobial activities of essential oil from leaves of the common sage Salvia officinalis L. from Abha, Saudi Arabia

2020 ◽  
Vol 14 (6) ◽  
pp. 261-270 ◽  
Author(s):  
Atef El Jery ◽  
Mudassir Hasan ◽  
Md Mamoon Rashid ◽  
Mohammed Khaloofah Al Mesfer ◽  
Mohd Danish ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Essential Oil ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Salvia Officinalis ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
The Common

AbstractBackgroundThe composition and activities of essential oil of common sage from Saudi Arabia have not yet been reported.ObjectivesTo analyze the composition and antibacterial and antioxidant activities of essential oil from leaves of the common sage Salvia officinalis L. from Abha, Saudi Arabia.MethodsEssential oil was extracted from the leaves of S. officinalis by hydrodistillation, and its composition was analyzed using gas chromatography and mass spectrometry. Phenolics and flavonoids were determined using gallic acid and quercetin standards. Antioxidant activity was determined using a 2,2-diphenyl-1-picrylhydrazyl radical scavenging method. Activity against various gram-positive and gram-negative bacteria was determined by disk diffusion and microdilution.ResultsThe yield of essential oil was 3.24 ± 0.55% (w/dry weight). Major compounds identified were camphor (20.3%), 1,8-cineole (15.0%), α-thujone (14.9%), viridiflorol (9.9%), carvone (6.2%), and β-thujone (5.7%). Phenolic content was 134.3 ± 17.61 μg/mL and flavonoid content was 119.5 ± 18.75 μg/mL. Antioxidant IC50 was 970 ± 5.5 μg/mL. The highest gram-positive antibacterial activity was for Bacillus subtilis and the highest gram-negative activity was for Escherichia coli. Minimum inhibitory concentrations ranged from 62.2 ± 3.9 to 1398.1 ± 50.7 μg/mL for gram-positive bacteria and from 323.4 ± 69.5 to 968.4 ± 120.6 μg/mL for gram-negative bacteria. Minimum bactericidal concentrations ranged from 120.3 ± 7.6 to 1387.4 ± 161.8 μg/mL for gram-positive bacteria and from 386 ± 8.3 to 1225.2 ± 100.9 μg/mL for gram-negative bacteria.ConclusionsEssential oil of S. officinalis L. from Abha, Saudi Arabia, showed compositional, antioxidant, and antibacterial properties generally consistent with essential oil of S. officinalis L. from other locations as reported in the literature.

Effect of Annealing Process on Natural Zeolites from Volcanic Ash for Environmental Applications: Structural and Morphological Characterizations

2016 ◽  
Vol 257 ◽  
pp. 233-236 ◽  
Author(s):  
Jorge A. Calderón ◽  
Sandra M. López ◽  
Heiddy P. Quiroz ◽  
Anderson Dussan
Keyword(s):  
Volcanic Ash ◽  
Scanning Electron Micrographs ◽  
Air Pollution Control ◽  
Natural Zeolites ◽  
X Ray Diffraction ◽  
Zeolite Type ◽  
Calcium Aluminosilicate ◽  
Xrd Patterns ◽  
Compositional Properties ◽  
Influence Of Hydration

This work presents a study of morphological, structural, and compositional properties of volcanic ash obtained from Nevado del Ruiz volcano in Colombia. The volcanic ash samples were subjected to hydration and annealing processes at atmospheric pressure to obtain zeolite-type natural structures for different applications, like water decontamination and air pollution control. The strong influence of hydration and annealing processes on the formation of zeolite phases was observed. Rietveld refinement was performed with X-ray diffraction (XRD) patterns, obtaining the presence of natural zeolites: gismondine, hydrated calcium aluminosilicate, litosite, clinoplitolite-Na, and phillipsite-K. From scanning electron micrographs, a morphological change of the ash sample was observed with increasing annealing temperature (290 K < T < 673 K). The surface was dominated by the presence of agglomerates or granules around 5 µm in size; other regions with tubular form or flake-type structures were observed with less presence on the surface ash, associated with the formation of the labradorite phase.

scholarly journals CHEMICAL COMPOSITION AND BIOLOGICAL ACTIVITY OF THE ESSENTIAL OIL OF WILD-GROWING Micromeria thymifolia (Scop.) Fritsch

2021 ◽  
Vol 20 (4) ◽  
pp. 69-76
Author(s):  
Marija Marin ◽  
Snežana Branković
Keyword(s):  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Gas Chromatography Mass Spectrometry ◽  
Gram Positive Bacteria ◽  
Flame Ionization ◽  
Dose Dependent

Essential oil (ЕО) from the leaves of wild-growing Micromeria thymifolia (Scop.) Fritsch (Lamiaceae) was screened for its chemical composition as well as its possible antioxidant and antibacterial properties. According to the gas chromatography – flame ionization detector (GC-FID) and gas chromatography – mass spectrometry (GC-MS) analyses, twenty-six compounds were identified, representing approximately 99.0% of the chromatographic area. The major component present in the essential oil was pulegone (44.8%), other main constituents were piperitone oxide (14.5%), iso-menthone (9.3%) and limonene (8.0%). The oil was tested for the antioxidant activity in 2,2-diphenyl-1-picryl hydrazyl (DPPH) assay and showed a dose-dependent free radical scavenging activity with EC50 value of 6.71 × 104 μg/ml. The antibacterial effect of the essential oil was tested against Gram-negative and Gram-positive bacteria. The essential oil of M. thymifolia showed antioxidant potential and antibacterial activity.

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