scholarly journals Incorporation of Elastin to Improve Polycaprolactone-Based Scaffolds for Skeletal Muscle via Electrospinning

Polymers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1501
Author(s):  
Victor Perez-Puyana ◽  
Paula Villanueva ◽  
Mercedes Jiménez-Rosado ◽  
Fernando de la Portilla ◽  
Alberto Romero
Keyword(s):  
Mechanical Properties ◽  
Skeletal Muscle ◽  
Cell Proliferation ◽  
Mechanical Stimulation ◽  
Muscle Regeneration ◽  
Skeletal Muscle Regeneration ◽  
Dynamic Mechanical ◽  
Fiber Size ◽  
Cell Incubation ◽  
Biological Tests

Skeletal muscle regeneration is increasingly necessary, which is reflected in the increasing number of studies that are focused on improving the scaffolds used for such regeneration, as well as the incubation protocol. The main objective of this work was to improve the characteristics of polycaprolactone (PCL) scaffolds by incorporating elastin to achieve better cell proliferation and biocompatibility. In addition, two cell incubation protocols (with and without dynamic mechanical stimulation) were evaluated to improve the activity and functionality yields of the regenerated cells. The results indicate that the incorporation of elastin generates aligned and more hydrophilic scaffolds with smaller fiber size. In addition, the mechanical properties of the resulting scaffolds make them adequate for use in both bioreactors and patients. All these characteristics increase the biocompatibility of these systems, generating a better interconnection with the tissue. However, due to the low maturation achieved in biological tests, no differences could be found between the incubation with and without dynamic mechanical stimulation.

scholarly journals PEDF-derived peptide promotes skeletal muscle regeneration through its mitogenic effect on muscle progenitor cells

2015 ◽  
Vol 309 (3) ◽  
pp. C159-C168 ◽  
Author(s):  
Tsung-Chuan Ho ◽  
Yi-Pin Chiang ◽  
Chih-Kuang Chuang ◽  
Show-Li Chen ◽  
Jui-Wen Hsieh ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cell Proliferation ◽  
Cyclin D1 ◽  
Satellite Cell ◽  
Muscle Regeneration ◽  
Muscle Fibers ◽  
Skeletal Muscle Regeneration ◽  
Satellite Cell Proliferation

In response injury, intrinsic repair mechanisms are activated in skeletal muscle to replace the damaged muscle fibers with new muscle fibers. The regeneration process starts with the proliferation of satellite cells to give rise to myoblasts, which subsequently differentiate terminally into myofibers. Here, we investigated the promotion effect of pigment epithelial-derived factor (PEDF) on muscle regeneration. We report that PEDF and a synthetic PEDF-derived short peptide (PSP; residues Ser93-Leu112) induce satellite cell proliferation in vitro and promote muscle regeneration in vivo. Extensively, soleus muscle necrosis was induced in rats by bupivacaine, and an injectable alginate gel was used to release the PSP in the injured muscle. PSP delivery was found to stimulate satellite cell proliferation in damaged muscle and enhance the growth of regenerating myofibers, with complete regeneration of normal muscle mass by 2 wk. In cell culture, PEDF/PSP stimulated C2C12 myoblast proliferation, together with a rise in cyclin D1 expression. PEDF induced the phosphorylation of ERK1/2, Akt, and STAT3 in C2C12 myoblasts. Blocking the activity of ERK, Akt, or STAT3 with pharmacological inhibitors attenuated the effects of PEDF/PSP on the induction of C2C12 cell proliferation and cyclin D1 expression. Moreover, 5-bromo-2′-deoxyuridine pulse-labeling demonstrated that PEDF/PSP stimulated primary rat satellite cell proliferation in myofibers in vitro. In summary, we report for the first time that PSP is capable of promoting the regeneration of skeletal muscle. The signaling mechanism involves the ERK, AKT, and STAT3 pathways. These results show the potential utility of this PEDF peptide for muscle regeneration.

Delayed angiogenesis and VEGF production in CCR2−/− mice during impaired skeletal muscle regeneration

2007 ◽  
Vol 293 (2) ◽  
pp. R651-R661 ◽  
Author(s):  
Oscar Ochoa ◽  
Dongxu Sun ◽  
Sara M. Reyes-Reyna ◽  
Lindsay L. Waite ◽  
Joel E. Michalek ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Regeneration ◽  
Capillary Density ◽  
Skeletal Muscle Regeneration ◽  
Mouse Strains ◽  
Vascular Endothelial ◽  
Wild Type ◽  
Fiber Size ◽  
Cc Chemokine Receptor 2 ◽  
Endothelial Growth Factor

The regulation of vascular endothelial growth factor (VEGF) levels and angiogenic events during skeletal muscle regeneration remains largely unknown. This study examined angiogenesis, VEGF levels, and muscle regeneration after cardiotoxin (CT)-induced injury in mice lacking the CC chemokine receptor 2 (CCR2). Muscle regeneration was significantly decreased in CCR2−/− mice as was the early accumulation of macrophages after injury. In both mouse strains, tissue VEGF was similar at baseline (no injections) and significantly decreased at day 3 post-CT. Tissue VEGF in wild-type (WT) mice was restored within 7 days postinjury but remained significantly reduced in CCR2−/− mice until day 21. Capillary density (capillaries/mm2) within regenerating muscle was maximal in WT mice at day 7 and double that of baseline muscle. In comparison, maximal capillary density in CCR2−/− mice occurred at 21 days postinjury. Maximal capillary density developed concurrent with the restoration of tissue VEGF in both strains. A highly significant, inverse relationship existed between the size of regenerated muscle fibers and capillaries per square millimeter. Although this relationship was comparable in WT and CCR2−/− animals, there was a significant decrease in the magnitude of this response in the absence of CCR2, reflecting the observation that regenerated muscle fiber size in CCR2−/− mice was only 50% of baseline at 42 days postinjury, whereas WT mice had attained baseline fiber size by day 21. Thus CCR2-dependent events in injured skeletal muscle, including impaired macrophage recruitment, contribute to restoration of tissue VEGF levels and the dynamic processes of capillary formation and muscle regeneration.

scholarly journals The circular RNA circCPE regulates myoblast development by sponging miR-138

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Wenxiu Ru ◽  
Ao Qi ◽  
Xuemei Shen ◽  
Binglin Yue ◽  
Xiaoyan Zhang ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cell Proliferation ◽  
Muscle Regeneration ◽  
Noncoding Rna ◽  
Muscle Development ◽  
Circular Rna ◽  
Inhibitory Effect ◽  
Skeletal Muscle Regeneration ◽  
Bovine Muscle

Abstract Background Skeletal muscle development, a long-term and complex process, is controlled by a set of the myogenic genes. Circular RNAs (circRNAs), a class of noncoding RNA, have been shown to regulate various biological processes. Recent studies indicate circRNAs may be involved in myogenesis, but the role and regulatory mechanism of circRNAs in myogenesis is largely unknown. In the present study, circCPE was firstly found to promote the bovine myoblast proliferation and inhibit cell apoptosis and differentiation by influencing the expression of FOXC1 in a miR138-mediated manner. And in vivo experiments revealed that overexpression of circCPE attenuates skeletal muscle regeneration. Results We identified a novel circular RNA circCPE by analyzing circRNAs sequencing data of bovine muscle tissue. Sequencing verification, RNase R treatment and Actinomycin D treatment confirmed the circular nature of circCPE in bovine muscle. Functional assays showed that overexpression of circCPE could inhibit bovine myoblast apoptosis and differentiation, as well as facilitate cell proliferation. Moreover, in vivo experiments revealed that overexpression of circCPE attenuates skeletal muscle regeneration. In consideration of circRNA action as miRNAs sponge, we found that circCPE harbors miR-138 binding sites and absorbed miR-138. Mechanistically, the rescue experiments showed that the overexpression of circCPE can counteract the inhibitory effect of miR-138 on the cell proliferation and the accelerated effects on the differentiation and apoptosis. Subsequently, we found that circCPE sequester the inhibitory effect of miR-138 on FOXC1 so as to involve in myogenesis. Conclusions Collectively, we constructed a novel circCPE/miR-138/FOXC1 regulatory network in bovine myogenesis, which further provide stronger evidence that circRNA involved in muscle development acting as miRNA sponge.

scholarly journals Angiotensin II Inhibits Satellite Cell Proliferation and Prevents Skeletal Muscle Regeneration

2013 ◽  
Vol 288 (33) ◽  
pp. 23823-23832 ◽  
Author(s):  
Tadashi Yoshida ◽  
Sarah Galvez ◽  
Sumit Tiwari ◽  
Bashir M. Rezk ◽  
Laura Semprun-Prieto ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cell Proliferation ◽  
Angiotensin Ii ◽  
Satellite Cell ◽  
Muscle Regeneration ◽  
Skeletal Muscle Regeneration ◽  
Satellite Cell Proliferation

scholarly journals The Effect of Gas6‐Axl Double Knockout on Satellite Cell Proliferation and Skeletal Muscle Regeneration After Injury

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Matthew J. Mervis ◽  
Marc S. Matsumura ◽  
Zachary E. Olsen ◽  
Kelsey M. Hirschi-Budge ◽  
Paul R. Reynolds ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cell Proliferation ◽  
Satellite Cell ◽  
Muscle Regeneration ◽  
Skeletal Muscle Regeneration ◽  
Double Knockout ◽  
Satellite Cell Proliferation

scholarly journals PPARδ regulates satellite cell proliferation and skeletal muscle regeneration

2011 ◽  
Vol 1 (1) ◽  
pp. 33 ◽  
Author(s):  
Alison R Angione ◽  
Chunhui Jiang ◽  
Dongning Pan ◽  
Yong-Xu Wang ◽  
Shihuan Kuang
Keyword(s):  
Skeletal Muscle ◽  
Cell Proliferation ◽  
Satellite Cell ◽  
Muscle Regeneration ◽  
Skeletal Muscle Regeneration ◽  
Satellite Cell Proliferation
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