scholarly journals Sensitive, Color-Indicating and Labeling-Free Multi-Detection Cholesteric Liquid Crystal Biosensing Chips for Detecting Albumin

Polymers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1463
Author(s):  
I-Te Wang ◽  
Yen-Hua Lee ◽  
Er-Yuan Chuang ◽  
Yu-Cheng Hsiao
Keyword(s):  
Liquid Crystal ◽  
Detection Limit ◽  
Bovine Serum Albumin ◽  
Light Intensity ◽  
Cholesteric Liquid Crystal ◽  
Microfluidic Chips ◽  
Alignment Layer ◽  
Novel Device ◽  
Antigen Antibody ◽  
Immune Detection

A novel device for cholesteric liquid crystal (CLC)-based microfluidic chips, accommodated in a polydimethylsiloxane material, was invented. In this device, the reorientation of the CLCs was consistently influenced by the surface of the four channel walls and adjacent CLCs. When the inside of the microchannel was coated with the alignment layer, the CLCs oriented homeotropically in a focal conic state under cross-polarizers. Once antigens had bound onto antibodies immobilized onto the orientation sheet-coated channel walls, the light intensity of the CLC molecules converted from a focal conic state to a bright planar state caused by disrupting the CLCs. By means of utilizing pressure-propelling flow, the attachment of antigen/antibody to the CLCs should be detectable within consecutive sequences. The multi-microfluidic CLC-based chips were verified by measuring bovine serum albumin (BSA) and immune complexes of pairs of BSA antigen/antibody. We showed that the multiple microfluidic immunoassaying can be used for measuring BSA and pairs of antigen/antibody BSA with a detection limit of about 1 ng/mL. The linear range is 0.1 μg/mL–1 mg/mL. A limit of immune detection of pairs of BSA antigens/antibodies was 10 ng/mL of BSA plus 1000 ng/mL of the anti-BSA antibodies was observed. According to this innovative creation of immunoassaying, an unsophisticated multi-detection device with CLC-based labeling-free microfluidic chips is presented.

scholarly journals Enhanced Bandwidth Broadening of Infrared Reflector Based on Polymer Stabilized Cholesteric Liquid Crystals with Poly(N-vinylcarbazole) Used as Alignment Layer

Polymers ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 2238
Author(s):  
Limin Zhang ◽  
Qiumei Nie ◽  
Xiao-Fang Jiang ◽  
Wei Zhao ◽  
Xiaowen Hu ◽  
...  
Keyword(s):  
Liquid Crystal ◽  
Liquid Crystals ◽  
Electric Field ◽  
Cholesteric Liquid Crystal ◽  
Critical Role ◽  
Cholesteric Liquid Crystals ◽  
Smart Window ◽  
Alignment Layer ◽  
Thermal And Electrical Properties ◽  
Polymer Stabilized

Alignment layer plays a critical role on liquid crystal (LC) conformation for most LC devices. Normally, polyimide (PI) or polyvinyl alcohol (PVA), characterized by their outstanding thermal and electrical properties, have been widely applied as the alignment layer to align LC molecules. Here, we used a semi-conductive material poly(N-vinylcarbazole) (PVK) as the alignment layer to fabricate the cholesteric liquid crystal (CLC) device and the polymer-stabilized cholesteric liquid crystals (PSCLC)-based infrared (IR) reflectors. In the presence of ultraviolet (UV) irradiation, there are hole–electron pairs generated in the PVK layer, which neutralizes the impurity electrons in the LC–PVK junction, resulting in the reduction in the built-in electric field in the LC device. Therefore, the operational voltage of the CLC device switching from cholesteric texture to focal conic texture decreases from 45 V to 30 V. For the PSCLC-based IR reflectors with the PVK alignment layer, at the same applied electric field, the reflection bandwidth is enhanced from 647 to 821 nm, ranging from 685 to 1506 nm in the IR region, which makes it attractive for saving energy as a smart window.

A Dye-Dispersed Liquid Crystal Smart Reflector

1994 ◽  
Vol 360 ◽  
Author(s):  
Ching-Chao Chang ◽  
Franklin Lonberg ◽  
Robert B. Meyer
Keyword(s):  
Liquid Crystal ◽  
Light Intensity ◽  
Negative Feedback ◽  
Incident Light ◽  
Cholesteric Liquid Crystal ◽  
Dye Molecules ◽  
Incident Light Intensity ◽  
Average Temperature ◽  
Pitch Length

AbstractWe report a liquid crystal smart reflector, consisting of a cholesteric liquid crystal doped with dye. Light entering the liquid crystal is absorbed by the dye, generating heat which raises the average temperature of the liquid crystal. This in turn changes the pitch length of the cholesteric liquid crystal, causing an increase in its reflectivity, and thus reducing the amount of light absorbed by the dye molecules. A negative feedback stabilized condition is reached, in which the reflectivity is proportional to the incident light intensity. This effect is demonstrated both theoretically and experimentally.

scholarly journals Label-Free, Color-Indicating, Polarizer-Free Dye-Doped Liquid Crystal Microfluidic Polydimethylsiloxane Biosensing Chips for Detecting Albumin

Polymers ◽  
2021 ◽  
Vol 13 (16) ◽  
pp. 2587
Author(s):  
Fu-Lun Chen ◽  
Hui-Tzung Luh ◽  
Yu-Cheng Hsiao
Keyword(s):  
Liquid Crystal ◽  
Immune Responses ◽  
The Novel ◽  
Microfluidic Chips ◽  
Label Free ◽  
Sequential Order ◽  
Alignment Layer ◽  
Polarized Microscopy ◽  
Pressure Driven Flow ◽  
Novel Design

We reveal a novel design for dye-doped liquid crystal (DDLC) microfluidic biosensing chips in the polydimethylsiloxane material. With this design chip, the orientation of DDLCs was affected by the interface between the walls of the channels and DDLCs. When the inside of a channel was coated with an N,N-dimethyl-n-octadecyl-3-aminopropyltrimethoxysilyl chloride (DMOAP) alignment layer, the DDLCs oriented homeotropically in a homeotropic (H) state under cross-polarized microscopy. After immobilization of antigens with antibodies on the alignment layer-coated microchannel walls, the optical intensity of the DDLC change from the dark H state to the bright planar (P) state. Using pressure-driven flow, the binding of antigens/antibodies to the DDLCs could be detected in an experimental sequential order. The microfluidic DDLCs were tested by detecting bovine serum albumin (BSA) and its immune-responses of antigens/antibodies. We proved that this immunoassay chip was able to detect BSA antigens/antibodies pairs with the detection limit about 0.5 µg/mL. The novel DDLC chip was shown to be a simple, multi-detection device, and label-free microfluidic chips are presented.

Localization of Intracellular Antigens in thin Sections with Ferritin Labeled Antibody

1970 ◽  
Vol 28 ◽  
pp. 174-175
Author(s):  
M.S. Shahrabadi ◽  
T. Yamamoto
Keyword(s):  
Bovine Serum Albumin ◽  
Bovine Serum ◽  
Antigenic Determinants ◽  
Conjugate Prior ◽  
Thin Sections ◽  
Specific Attachment ◽  
Intracellular Antigen ◽  
Antigen Antibody ◽  
Ideal Method ◽  
The Ideal

The technique of labeling of macromolecules with ferritin conjugated antibody has been successfully used for extracellular antigen by means of staining the specimen with conjugate prior to fixation and embedding. However, the ideal method to determine the location of intracellular antigen would be to do the antigen-antibody reaction in thin sections. This technique contains inherent problems such as the destruction of antigenic determinants during fixation or embedding and the non-specific attachment of conjugate to the embedding media. Certain embedding media such as polyampholytes (2) or cross-linked bovine serum albumin (3) have been introduced to overcome some of these problems.

Dispersion of proton spin-lattice relaxation in a cholesteric liquid crystal

1983 ◽  
Vol 44 (10) ◽  
pp. 1179-1184 ◽  
Author(s):  
M. Vilfan ◽  
R. Blinc ◽  
J. Dolinšek ◽  
M. Ipavec ◽  
G. Lahajnar ◽  
...  
Keyword(s):  
Liquid Crystal ◽  
Cholesteric Liquid Crystal ◽  
Lattice Relaxation ◽  
Proton Spin ◽  
Spin Lattice Relaxation ◽  
Spin Lattice

Enhancement of Contrast Ratio by Using Ferroelectric Nanoparticles in the Alignment Layer of Liquid Crystal Display

2008 ◽  
Vol 47 (6) ◽  
pp. 4751-4754 ◽  
Author(s):  
Sudarshan Kundu ◽  
Mitsuhiro Akimoto ◽  
Itaru Hirayama ◽  
Masaru Inoue ◽  
Shunsuke Kobayashi ◽  
...  
Keyword(s):  
Liquid Crystal ◽  
Liquid Crystal Display ◽  
Contrast Ratio ◽  
Alignment Layer

scholarly journals Flow-assisted self-healing of the helical structure in a cholesteric liquid crystal

2021 ◽  
Vol 155 (5) ◽  
pp. 054903
Author(s):  
Jan-Christoph Eichler ◽  
Robert A. Skutnik ◽  
Marco G. Mazza ◽  
Martin Schoen
Keyword(s):  
Liquid Crystal ◽  
Cholesteric Liquid Crystal ◽  
Helical Structure ◽  
Self Healing

scholarly journals The temperature dependence of the helical pitch in a cholesteric liquid crystal

2021 ◽  
pp. e1881638
Author(s):  
Robert A. Skutnik ◽  
Jan-Christoph Eichler ◽  
Marco G. Mazza ◽  
Martin Schoen
Keyword(s):  
Liquid Crystal ◽  
Temperature Dependence ◽  
Cholesteric Liquid Crystal ◽  
Helical Pitch
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