scholarly journals Noninvasive Tracking of mPEG-poly(Ala) Hydrogel-Embedded MIN6 Cells after Subcutaneous Transplantation in Mice

Polymers ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 885
Author(s):  
Jyuhn-Huarng Juang ◽  
Hsiu-Chao Lin ◽  
Chen-Yi Chen ◽  
Chen-Wei Kao ◽  
Chen-Ling Chen ◽  
...  
Keyword(s):  
Bioluminescence Imaging ◽  
Superparamagnetic Iron Oxide ◽  
Cell Encapsulation ◽  
Mr Images ◽  
Min6 Cells ◽  
In Vivo Experiments ◽  
Subcutaneous Transplantation ◽  
Poly Ethylene ◽  
Glycol Methyl Ether

Recently, we demonstrated the feasibility of subcutaneous transplantation of MIN6 cells embedded in a scaffold with poly(ethylene glycol) methyl ether (mPEG)-poly(Ala) hydrogels. In this study, we further tracked these grafts using magnetic resonance (MR) and bioluminescence imaging. After being incubated overnight with chitosan-coated superparamagnetic iron oxide (CSPIO) nanoparticles and then mixed with mPEG-poly(Ala) hydrogels, MIN6 cells appeared as dark spots on MR scans. For in vivo experiments, we transfected MIN6 cells with luciferase and/or incubated them overnight with CSPIO overnight; 5 × 106 MIN6 cells embedded in mPEG-poly(Ala) hydrogels were transplanted into the subcutaneous space of each nude mouse. The graft of CSPIO-labeled MIN6 cells was visualized as a distinct hypointense area on MR images located at the implantation site before day 21. However, this area became hyperintense on MR scans for up to 64 days. In addition, positive bioluminescence images were also observed for up to 64 days after transplantation. The histology of removed grafts showed positive insulin and iron staining. These results indicate mPEG-poly(Ala) is a suitable scaffold for β-cell encapsulation and transplantation. Moreover, MR and bioluminescence imaging are useful noninvasive tools for detecting and monitoring mPEG-poly(Ala) hydrogel-embedded MIN6 cells at a subcutaneous site.

scholarly journals In Vivo Distribution of Poly(ethylene glycol) Functionalized Iron Oxide Nanoclusters: An Ultrastructural Study

Nanomaterials ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2184
Author(s):  
Maria Suciu ◽  
Claudiu Mirescu ◽  
Izabell Crăciunescu ◽  
Sergiu Gabriel Macavei ◽  
Cristian Leoștean ◽  
...  
Keyword(s):  
Iron Oxide ◽  
De Novo ◽  
Superparamagnetic Iron Oxide ◽  
In Vivo Distribution ◽  
Transmission Electron ◽  
Blood And Urine Samples ◽  
Poly Ethylene ◽  
Exposure Evaluation

The in vivo distribution of 50 nm clusters of polyethylene glycol-conjugated superparamagnetic iron oxide nanoparticles (SPIONs-PEG) was conducted in this study. SPIONs-PEG were synthesized de novo, and their structure and paramagnetic behaviors were analyzed by specific methods (TEM, DLS, XRD, VSM). Wistar rats were treated with 10 mg Fe/kg body weight SPIONs-PEG and their organs and blood were examined at two intervals for short-term (15, 30, 60, 180 min) and long-term (6, 12, 24 h) exposure evaluation. Most exposed organs were investigated through light and transmission electron microscopy, and blood and urine samples were examined through fluorescence spectrophotometry. SPIONs-PEG clusters entered the bloodstream after intraperitoneal and intravenous administrations and ended up in the urine, with the highest clearance at 12 h. The skin and spleen were within normal histological parameters, while the liver, kidney, brain, and lungs showed signs of transient local anoxia or other transient pathological affections. This study shows that once internalized, the synthesized SPIONs-PEG disperse well through the bloodstream with minor to nil induced tissue damage, are biocompatible, have good clearance, and are suited for biomedical applications.

scholarly journals Synthesis of Superparamagnetic Iron Oxide Nanoparticles Modified with MPEG-PEI via Photochemistry as New MRI Contrast Agent

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Yancong Zhang ◽  
Lianying Zhang ◽  
Xinfeng Song ◽  
Xiangling Gu ◽  
Hanwen Sun ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Magnetic Resonance ◽  
Ethylene Glycol ◽  
Iron Oxide Nanoparticles ◽  
Methyl Ether ◽  
Superparamagnetic Iron Oxide ◽  
Oxide Nanoparticles ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene ◽  
Glycol Methyl Ether

Novel method for synthesis of superparamagnetic iron oxide nanoparticles (SPIONs) coated with polyethylenimine (PEI) and modified with poly(ethylene glycol) methyl ether (MPEG), MPEG-PEI-SPIONs, was developed. PEI-SPIONs were successfully prepared in aqueous system via photochemistry, and their surface was modified with poly(ethylene glycol) methyl ether (MPEG). The so-obtained MPEG-PEI-SPIONs had a uniform hydrodynamic particle size of 34 nm. The successful coating of MPEG-PEI on the SPIONs was ascertained from FT-IR analysis, and the PEI and MPEG fractions in MPEG-PEI-SPIONs were calculated to account for 31% and 12%, respectively. Magnetic measurement revealed that the saturated magnetization of MPEG-PEI-SPIONs reached 46 emu/g and the nanoparticles showed the characteristic of being superparamagnetic. The stability experiment revealed that the MPEG-PEI modification improved the nanoparticles stability greatly.T2 relaxation measurements showed that MPEG-PEI-SPIONs show similarR2 value to the PEI-SPIONs. TheT2-weighted magnetic resonance imaging (MRI) of MPEG-PEI-SPIONs showed that the magnetic resonance signal was enhanced significantly with increasing nanoparticle concentration in water. These results indicated that the MPEG-PEI-SPIONs had great potential for application in MRI.

Delivery of Ferulic Acid with PEGylated DiphenylalanineBased Nanoparticles for the Treatment of Arthritis in Early Stage

2021 ◽  
Vol 17 (3) ◽  
pp. 357-368
Author(s):  
Liming He ◽  
Yingchun Zeng ◽  
Xianyan Qin ◽  
Lihua Pan ◽  
Tao Chen ◽  
...  
Keyword(s):  
Ferulic Acid ◽  
Early Stage ◽  
Oxygen Species ◽  
Poly Ethylene Glycol ◽  
Anti Inflammatory ◽  
Poly Ethylene ◽  
Favorable Safety ◽  
Arthritic Joints ◽  
Glycol Methyl Ether

Ferulic acid (FA), an active component extracted from Chinese medicine, shows excellent anti-inflammatory properties and favorable safety in various animal models. However, the application of FA as an anti-inflammatory drug is hindered by its instability and short half-life in vivo . In this paper, we synthesize PEGylated diphenylalanine nanoparticles by using glutaraldehyde (GTA) as a cross-linker of diphenylalanine NH2 -Phe–Phe-COOH and poly(ethylene glycol) methyl ether amine (PEG5k -NH2). The PEGylated Phe–Phe nanoparticles are used to deliver FA for the treatment of Rheumatoid arthritis (RA). We find that the FA-loaded PEGylated Phe–Phe nanoparticles are biocompatible and inhibit the production of reactive oxygen species (ROS) from cells effectively. After being intravenously administrated in vivo , the FA-loaded PEGylated Phe–Phe nanoparticles show prolonged circulation time and accumulate in arthritic joints. More importantly, we show that the pre-arthritis treatment with the FA-loaded PEGylated Phe–Phe nanoparticles can significantly block the progression of RA.

An Injectable Double-Network Hydrogel for Cell Encapsulation

2016 ◽  
Vol 69 (4) ◽  
pp. 388 ◽  
Author(s):  
Pei Lin Chee ◽  
Lakshmi Lakshmanan ◽  
Shan Jiang ◽  
Hongye Ye ◽  
Dan Kai ◽  
...  
Keyword(s):  
Alginic Acid ◽  
High Strain ◽  
Cell Encapsulation ◽  
Cytotoxicity Test ◽  
Poly Ethylene Glycol ◽  
Double Network ◽  
Cell Compatibility ◽  
A Cell ◽  
Poly Ethylene ◽  
Glycol Methyl Ether

Further developing on the technique originally intended for the purpose of forming tough hydrogels, we showed in this study that the double-network system can also be used to synthesize an injectable gel. The gel was made up of poly(ethylene glycol) methyl ether methacrylate, sodium alginic acid, and calcium chloride, and two networks, consisting of ionic and covalent networks, were found to co-exist in the gel. Additionally, the rheology studies showed that the mechanical properties of the gel only deteriorated under high strain, demonstrating the robustness of the gel upon injection. The results of a cell cytotoxicity test and a preliminary cell encapsulation study were promising, showing good cell compatibility and thus suggesting that the hydrogels could potentially be used for cell delivery.

In vitro and In vivo Blood Compatibility of Concentrated Polymer Brushes

2021 ◽  
Author(s):  
Chiaki Yoshikawa ◽  
Shinya Hattori ◽  
Chih-Feng Huang ◽  
Hisatoshi Kobayashi ◽  
Masaru Tanaka
Keyword(s):  
Ethylene Glycol ◽  
Methyl Ether ◽  
Polymer Brushes ◽  
Blood Compatibility ◽  
Hydroxyethyl Methacrylate ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene ◽  
Glycol Methyl Ether

Concentrated polymer brushes (CPBs) and semi-dilute polymer brushes (SDPBs) of poly(2-hydroxyethyl methacrylate), poly(2-hydroxyethyl acrylate), poly[poly(ethylene glycol) methyl ether methacrylate)] (PPEGMA) and poly(2-methoxyetyl acrylate) were prepared on silica particles and silicon...

scholarly journals Improved Pharmacokinetics of Icariin (ICA) within Formulation of PEG-PLLA/PDLA-PNIPAM Polymeric Micelles

Pharmaceutics ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 51 ◽  
Author(s):  
Lu-Ying Han ◽  
Yun-Long Wu ◽  
Chun-Yan Zhu ◽  
Cai-Sheng Wu ◽  
Chun-Rong Yang
Keyword(s):  
Lactic Acid ◽  
Polymeric Micelles ◽  
Polymeric Micelle ◽  
Pluronic F127 ◽  
In Vivo Experiments ◽  
Poly Ethylene ◽  
Metabolites Production ◽  
New Formulation

Icariin (ICA) is a major flavonoid that contains the active compound Epimedii Folium. However, ICA’s pharmacokinetic characteristics remain unsatisfactory due to its low bioavailability, and hence limited drugability. In order to improve its pharmacokinetics and achieve prolonged blood circulation time, a novel polymeric micelle, made of the self-assembled micelle between poly (ethylene glycol)-poly (L-lactic acid) (PEG-PLLA) and poly (D-lactic acid)-poly(N-isopropylacrylamide) (PDLA-PNIPAM), was designed to encapsulate ICA. Our experimental results showed that this polymeric micelle formulation of ICA exhibited uniform nano-size distribution and high stability within 48 h. The new formulation also allowed sustained ICA release in an in vitro drug release study. Furthermore, in vivo experiments revealed that ICA bioavailability in the PEG-PLLA/PDLA-PNIPAM polymeric micelle formulation was significantly higher compared to ICA alone, or ICA in the traditional Pluronic F127 micelle formulation. Finally, we show that metabolite analysis confirmed that ICA within the PEG-PLLA/PDLA-PNIPAM polymeric micelle formulation provided better drug protection, reduced drug metabolites production, and decreased undesired first-pass effects. Overall, these data show that ICA within PEG-PLLA/PDLA-PNIPAM polymeric micelle formulation exhibit advantages, in terms of improved physicochemical properties, sustained release of ICA in vitro, and improved bioavailability of ICA in vivo, which represent a feasible approach for improving the drugability of pharmaceutical small molecules with low bioavailability or poor stability.

Measurement of Lipid Content in Gallbladder Bile Using in- and opposed-phase MR Images and in vivo Proton MR Spectroscopy

2002 ◽  
Vol 46 (2) ◽  
pp. 127
Author(s):  
Sun Jin Hur ◽  
Seok Hwan Shin ◽  
Geum Nan Jee ◽  
Eun Joo Yun ◽  
Soon Gu Cho ◽  
...  
Keyword(s):  
Lipid Content ◽  
Mr Spectroscopy ◽  
Gallbladder Bile ◽  
Mr Images ◽  
Proton Mr Spectroscopy

Stem Cell Cure for Kidney Injury: Therapy to Solve Most Complex Issues in Renal System

2020 ◽  
Author(s):  
Prithiv K R Kumar
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Kidney Injury ◽  
Cell Types ◽  
Tissue Remodelling ◽  
Major Health Problem ◽  
In Vivo Experiments ◽  
Renal Regeneration ◽  
Induced Pluripotent

Renal failure is a major health problem. The mortality rate remain high despite of several therapies. The most complex of the renal issues are solved through stem cells. In this review, different mechanism for cure of chronic kidney injury along with cell engraftment incorporated into renal structures will be analysed. Paracrine activities of embryonic or induced Pluripotent stem cells are explored on the basis of stem cell-induced kidney regeneration. Several experiments have been conducted to advance stem cells to ensure the restoration of renal functions. More vigour and organised protocols for delivering stem cells is a possibility for advancement in treatment of renal disease. Also there is a need for pressing therapies to replicate the tissue remodelling and cellular repair processes suitable for renal organs. Stem cells are the undifferentiated cells that have the ability to multiply into several cell types. In vivo experiments on animal’s stem cells have shown significant improvements in the renal regeneration and functions of organs. Nevertheless more studies show several improvements in the kidney repair due to stem cell regeneration.

In vivo Experiments of Natural Products Protection of Antagonistic Effects of Lead on Iron

2018 ◽  
Vol 68 (12) ◽  
pp. 2747-2751
Author(s):  
Marioara Nicula ◽  
Nicolae Pacala ◽  
Lavinia Stef ◽  
Ioan Pet ◽  
Dorel Dronca ◽  
...  
Keyword(s):  
Aquatic Environment ◽  
Iron Homeostasis ◽  
Iron Concentration ◽  
Antagonistic Effect ◽  
Tissue Samples ◽  
Antagonistic Effects ◽  
In Vivo Experiments ◽  
Living Organisms ◽  
Toxic Potential

Living organisms take nutrients from the environment, and together with them, substances with toxic potential � such as heavy metals. Lead is one common metal pollutant especially in aquatic environment, from where the fish can be intoxicated very easily. Bioavailability, distribution, toxic action, synergistic and antagonistic effects are characteristics which can alter the fish health. Our experimental study followed the effects of lead overload in water on iron distribution, in different tissues sample Carassius gibelio Bloch fish. We performed the experiment in four different fish groups: control C; lead � Pb (administration of lead in water 0.075mg/mL of water, as Pb(NO3)2 x � H2O); lead (the same dose) and 2% of freeze-dry garlic incorporated into fishes� food � Pb+garlic; lead (the same dose) and 2% chlorella incorporated into fishes� food � Pb+chlorella, for 21 consecutive days. The iron concentration was analysed with AAS (Atomic Absorption Spectroscopy) from gills, muscle, skin (and scales), intestine, liver, heart, brain, ovary, testicles, and kidney. The obtained data presented a significantly decrease of iron content in all tested tissue samples that demonstrated, alteration of iron homeostasis, explained by a strong antagonistic effect of lead on iron. Our experiment showed that biologic active principles from garlic and chlorella act like natural protectors, and potentiate the iron deficiency even in the case of lead overload in aquatic environment, for fish.

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